Evidence of off-target effects associated with long dsRNAs in Drosophila melanogaster cell-based assays

To evaluate the specificity of long dsRNAs used in high-throughput RNA interference (RNAi) screens performed at the Drosophila RNAi Screening Center (DRSC), we performed a global analysis of their activity in 30 genome-wide screens completed at our facility. Notably, our analysis predicts that dsRNA...

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Veröffentlicht in:	Nature methods 2006-10, Vol.3 (10), p.833-838
Hauptverfasser:	Kulkarni, Meghana M, Booker, Matthew, Silver, Serena J, Friedman, Adam, Hong, Pengyu, Perrimon, Norbert, Mathey-Prevot, Bernard
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Bioinformatics Biological assay Biological Microscopy Biological Techniques Biomedical and Life Sciences Biomedical Engineering/Biotechnology Drosophila Drosophila melanogaster - cytology Drosophila melanogaster - genetics False Positive Reactions Gene Library Genetic aspects Genetic Testing - methods Genetic Testing - standards Genomics Hybridization Insects Life Sciences Methods Physiological aspects Proteomics Research methodology Ribonucleic acid RNA RNA Interference RNA, Double-Stranded - genetics Sensitivity and Specificity
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