β-Catenin Regulates Myogenesis by Relieving I-mfa-Mediated Suppression of Myogenic Regulatory Factors in P19 Cells
Wnt/β-catenin signaling plays a critical role in embryonic myogenesis. Here we show that, in P19 embryonic carcinoma stem cells, Wnt/β-catenin signaling initiates the myogenic process depends on β-catenin-mediated relief of I-mfa (inhibitor of MyoD Family a) suppression of myogenic regulatory factor...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 2005-11, Vol.102 (48), p.17378-17383 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | Weijun Pan Yingying Jia Jiyong Wang Donglei Tao Xiaoqing Gan Tsiokas, Leonidas Jing, Naihe Wu, Dianqing Li, Lin Roeland Nusse |
| description | Wnt/β-catenin signaling plays a critical role in embryonic myogenesis. Here we show that, in P19 embryonic carcinoma stem cells, Wnt/β-catenin signaling initiates the myogenic process depends on β-catenin-mediated relief of I-mfa (inhibitor of MyoD Family a) suppression of myogenic regulatory factors (MRFs). We found that β-catenin interacted with I-mfa and that the interaction was enhanced by Wnt3a. In addition, we found that the interaction between β-catenin and I-mfa was able to attenuate the interaction of I-mfa with MRFs, relieve I-mfa-mediated suppression of the transcriptional activity and cytosolic sequestration of MRFs, and initiate myogenesis in a P19 myogenic model system that expresses exogenous myogenin. This work reveals a mechanism for the regulation of MRFs during myogenesis by elucidating a β-catenin-mediated, but lymphoid enhancing factor-1/T cell factor independent, mechanism in regulation of myogenic fate specification and differentiation of P19 mouse stem cells. |
| doi_str_mv | 10.1073/pnas.0505922102 |
| format | Article |
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Here we show that, in P19 embryonic carcinoma stem cells, Wnt/β-catenin signaling initiates the myogenic process depends on β-catenin-mediated relief of I-mfa (inhibitor of MyoD Family a) suppression of myogenic regulatory factors (MRFs). We found that β-catenin interacted with I-mfa and that the interaction was enhanced by Wnt3a. In addition, we found that the interaction between β-catenin and I-mfa was able to attenuate the interaction of I-mfa with MRFs, relieve I-mfa-mediated suppression of the transcriptional activity and cytosolic sequestration of MRFs, and initiate myogenesis in a P19 myogenic model system that expresses exogenous myogenin. This work reveals a mechanism for the regulation of MRFs during myogenesis by elucidating a β-catenin-mediated, but lymphoid enhancing factor-1/T cell factor independent, mechanism in regulation of myogenic fate specification and differentiation of P19 mouse stem cells.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.0505922102</identifier><identifier>PMID: 16301527</identifier><language>eng</language><publisher>United States: National Academy of Sciences</publisher><subject>Animals ; Antibodies ; beta Catenin - metabolism ; Biological Sciences ; Biology ; Cancer ; Cell Differentiation - physiology ; Cell Fractionation ; Cell Line, Tumor ; Cell lines ; Cellular differentiation ; Embryos ; Immunoprecipitation ; Mice ; Microscopy, Fluorescence ; Muscle development ; Muscle Development - physiology ; Myogenic Regulatory Factors - metabolism ; Plasmids ; RNA, Small Interfering - genetics ; Rodents ; Signal Transduction - physiology ; Skeletal muscle ; Small interfering RNA ; Stem cells ; Stem Cells - metabolism ; Stem Cells - physiology ; T lymphocytes ; Transcriptional regulatory elements ; Two-Hybrid System Techniques</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 2005-11, Vol.102 (48), p.17378-17383</ispartof><rights>Copyright 2005 National Academy of Sciences of the United States of America</rights><rights>Copyright National Academy of Sciences Nov 29, 2005</rights><rights>Copyright © 2005, The National Academy of Sciences 2005</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c529t-faf2ebc63e7515be854b53454b0fb155ce03c7133687d1554d6c8b45b0c075413</citedby><cites>FETCH-LOGICAL-c529t-faf2ebc63e7515be854b53454b0fb155ce03c7133687d1554d6c8b45b0c075413</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/102/48.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/4152486$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/4152486$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,315,728,781,785,804,886,27926,27927,53793,53795,58019,58252</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16301527$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Weijun Pan</creatorcontrib><creatorcontrib>Yingying Jia</creatorcontrib><creatorcontrib>Jiyong Wang</creatorcontrib><creatorcontrib>Donglei Tao</creatorcontrib><creatorcontrib>Xiaoqing Gan</creatorcontrib><creatorcontrib>Tsiokas, Leonidas</creatorcontrib><creatorcontrib>Jing, Naihe</creatorcontrib><creatorcontrib>Wu, Dianqing</creatorcontrib><creatorcontrib>Li, Lin</creatorcontrib><creatorcontrib>Roeland Nusse</creatorcontrib><title>β-Catenin Regulates Myogenesis by Relieving I-mfa-Mediated Suppression of Myogenic Regulatory Factors in P19 Cells</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>Wnt/β-catenin signaling plays a critical role in embryonic myogenesis. Here we show that, in P19 embryonic carcinoma stem cells, Wnt/β-catenin signaling initiates the myogenic process depends on β-catenin-mediated relief of I-mfa (inhibitor of MyoD Family a) suppression of myogenic regulatory factors (MRFs). We found that β-catenin interacted with I-mfa and that the interaction was enhanced by Wnt3a. In addition, we found that the interaction between β-catenin and I-mfa was able to attenuate the interaction of I-mfa with MRFs, relieve I-mfa-mediated suppression of the transcriptional activity and cytosolic sequestration of MRFs, and initiate myogenesis in a P19 myogenic model system that expresses exogenous myogenin. This work reveals a mechanism for the regulation of MRFs during myogenesis by elucidating a β-catenin-mediated, but lymphoid enhancing factor-1/T cell factor independent, mechanism in regulation of myogenic fate specification and differentiation of P19 mouse stem cells.</description><subject>Animals</subject><subject>Antibodies</subject><subject>beta Catenin - metabolism</subject><subject>Biological Sciences</subject><subject>Biology</subject><subject>Cancer</subject><subject>Cell Differentiation - physiology</subject><subject>Cell Fractionation</subject><subject>Cell Line, Tumor</subject><subject>Cell lines</subject><subject>Cellular differentiation</subject><subject>Embryos</subject><subject>Immunoprecipitation</subject><subject>Mice</subject><subject>Microscopy, Fluorescence</subject><subject>Muscle development</subject><subject>Muscle Development - physiology</subject><subject>Myogenic Regulatory Factors - metabolism</subject><subject>Plasmids</subject><subject>RNA, Small Interfering - genetics</subject><subject>Rodents</subject><subject>Signal Transduction - physiology</subject><subject>Skeletal muscle</subject><subject>Small interfering RNA</subject><subject>Stem cells</subject><subject>Stem Cells - metabolism</subject><subject>Stem Cells - physiology</subject><subject>T lymphocytes</subject><subject>Transcriptional regulatory elements</subject><subject>Two-Hybrid System Techniques</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkctu1DAUhi0EokNhzQYhiwUSi7THd2eDhEYUKrUCcVlbjuMMHmXsIU4q5rV4EJ4JjyZ0gE03Pr58_28f_wg9JXBGQLHzbbT5DASImlIC9B5aEKhJJXkN99ECgKpKc8pP0KOc1wBQCw0P0QmRDIigaoHyr5_V0o4-hog_-dXUl3nG17u08tHnkHGzK_t98DchrvBltelsde3bULAWf56228HnHFLEqZtVwf0xSsMOX1hXasbF_iOp8dL3fX6MHnS2z_7JXE_R14u3X5bvq6sP7y6Xb64qJ2g9Vp3tqG-cZF4JIhqvBW8E42WEriFCOA_MKcKY1Kota95KpxsuGnCgBCfsFL0--G6nZuNb5-M42N5sh7Cxw84kG8y_JzF8M6t0YwitlZS8GLycDYb0ffJ5NJuQXWnBRp-mbKTWXDAt7gRJrUBxpgv44j9wnaYhll8wFAirGYAs0PkBckPKefDd7ZMJmH3sZh-7OcZeFM__7vTIzzkX4NUM7JVHO2q4NkQxpU039f3of4yFxXewBXl2QNa5pHvL8HIX15L9BgaLy-E</recordid><startdate>20051129</startdate><enddate>20051129</enddate><creator>Weijun Pan</creator><creator>Yingying Jia</creator><creator>Jiyong Wang</creator><creator>Donglei Tao</creator><creator>Xiaoqing Gan</creator><creator>Tsiokas, Leonidas</creator><creator>Jing, Naihe</creator><creator>Wu, Dianqing</creator><creator>Li, Lin</creator><creator>Roeland Nusse</creator><general>National Academy of Sciences</general><general>National Acad Sciences</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20051129</creationdate><title>β-Catenin Regulates Myogenesis by Relieving I-mfa-Mediated Suppression of Myogenic Regulatory Factors in P19 Cells</title><author>Weijun Pan ; 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Here we show that, in P19 embryonic carcinoma stem cells, Wnt/β-catenin signaling initiates the myogenic process depends on β-catenin-mediated relief of I-mfa (inhibitor of MyoD Family a) suppression of myogenic regulatory factors (MRFs). We found that β-catenin interacted with I-mfa and that the interaction was enhanced by Wnt3a. In addition, we found that the interaction between β-catenin and I-mfa was able to attenuate the interaction of I-mfa with MRFs, relieve I-mfa-mediated suppression of the transcriptional activity and cytosolic sequestration of MRFs, and initiate myogenesis in a P19 myogenic model system that expresses exogenous myogenin. This work reveals a mechanism for the regulation of MRFs during myogenesis by elucidating a β-catenin-mediated, but lymphoid enhancing factor-1/T cell factor independent, mechanism in regulation of myogenic fate specification and differentiation of P19 mouse stem cells.</abstract><cop>United States</cop><pub>National Academy of Sciences</pub><pmid>16301527</pmid><doi>10.1073/pnas.0505922102</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Antibodies beta Catenin - metabolism Biological Sciences Biology Cancer Cell Differentiation - physiology Cell Fractionation Cell Line, Tumor Cell lines Cellular differentiation Embryos Immunoprecipitation Mice Microscopy, Fluorescence Muscle development Muscle Development - physiology Myogenic Regulatory Factors - metabolism Plasmids RNA, Small Interfering - genetics Rodents Signal Transduction - physiology Skeletal muscle Small interfering RNA Stem cells Stem Cells - metabolism Stem Cells - physiology T lymphocytes Transcriptional regulatory elements Two-Hybrid System Techniques |
| title | β-Catenin Regulates Myogenesis by Relieving I-mfa-Mediated Suppression of Myogenic Regulatory Factors in P19 Cells |
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