Bullous pemphigoid in a patient with psoriasis during the course of PUVA therapy: study by ELISA test

A 65‐year‐old woman had a history of deep vein thrombosis and depression. Psoriasis was diagnosed in 1986 and various topical and systemic therapies, singly or in combination, were prescribed: tar, topical corticosteroids, cyclosporine, etretinate, and methotrexate. Two courses of oral and one course of bath psoralen plus UVA (PUVA) therapy (cumulative dose, 467 J/cm2) and UVB (2.96 J/cm2) had been given. In January 1999, she developed a flare of generalized psoriasis. In May 1999, therapy with PUVA (8‐methoxypsoralen) plus topical acetonide triamcinolone 0.1% was initiated. At the time, she was taking acenocoumarol, lorazepam, and hydroxyzine chlorhydrate. In August 1999, at session 30, when the dose of UVA was 9 J/cm2, and the total dose was 205 J/cm2, a bulla appeared on the dorsum of the toe and was controlled with topical antibiotics. Five further sessions of PUVA were given and a generalized itching bullous eruption appeared all over the body. PUVA was stopped and the patient was hospitalized. On physical examination, extensive psoriatic plaques plus vesicles and bullae on the normal skin and on psoriatic lesions were observed all over the body (Fig. 1). 1 Clinical aspect of the bullae on the psoriatic plaques Histopathologic study of a lesion showed a subepidermal vesicle containing fibrin, neutrophils, and a few eosinophils. No sunburn cells were observed (Fig. 2). 2 Histopathologic aspect of a lesion, showing a subepidermal vesicle containing fibrin, neutrophils, and a few eosinophils (hematoxylin and eosin, ×200) The direct immunofluorescence (DIF) test of perilesional uninvolved skin revealed immunoglobulin G (IgG) (Fig. 3) and C3 at the dermal–epidermal junction. The DIF study using the patient's skin, previously treated with 1 m NaCl, localized the IgG at both the epidermal and dermal sides of the basement membrane zone (Fig. 4). 3 Direct immunofluorescence test of uninvolved skin showing linear deposits of immunoglobulin G (IgG) at the dermal–epidermal junction (×400) 4 Direct immunofluorescence test showing immunoglobulin G (IgG) at the epidermal and dermal sides of the 1 m NaCl‐induced vesicle (×400) Bullous pemphigoid (BP) was diagnosed and therapy with prednisone (60 mg/day) was started. The disease was well controlled in 3 weeks. The dose of prednisone was tapered and stopped 20 months later, without any recurrence. Study of the antibodies by the indirect immunofluorescence (IIF) test, using monkey esophagus and guinea pig as substrate,