Spatial gene expression in the T-stage mouse metanephros
The E11.5 mouse metanephros is comprised of a T-stage ureteric epithelial tubule sub-divided into tip and trunk cells surrounded by metanephric mesenchyme (MM). Tip cells are induced to undergo branching morphogenesis by the MM. In contrast, signals within the mesenchyme surrounding the trunk preven...
Gespeichert in:
| Veröffentlicht in: | Gene Expression Patterns 2006-10, Vol.6 (8), p.807-825 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 825 |
|---|---|
| container_issue | 8 |
| container_start_page | 807 |
| container_title | Gene Expression Patterns |
| container_volume | 6 |
| creator | Caruana, Georgina Cullen-McEwen, Luise Nelson, Amy L. Kostoulias, Xenia Woods, Kyra Gardiner, Brooke Davis, Melissa J. Taylor, Darrin F. Teasdale, Rohan D. Grimmond, Sean M. Little, Melissa H. Bertram, John F. |
| description | The E11.5 mouse metanephros is comprised of a T-stage ureteric epithelial tubule sub-divided into tip and trunk cells surrounded by metanephric mesenchyme (MM). Tip cells are induced to undergo branching morphogenesis by the MM. In contrast, signals within the mesenchyme surrounding the trunk prevent ectopic branching of this region. In order to identify novel genes involved in the molecular regulation of branching morphogenesis we compared the gene expression profiles of isolated tip, trunk and MM cells using Compugen mouse long oligo microarrays. We identified genes enriched in the tip epithelium,
sim-1,
Arg2,
Tacstd1,
Crlf-1 and
BMP7; genes enriched in the trunk epithelium,
Innp1, Itm2b, Mkrn1, SPARC, Emu2 and
Gsta3 and genes spatially restricted to the mesenchyme surrounding the trunk,
CSPG2 and
CV-2, with overlapping and complimentary expression to
BMP4, respectively. This study has identified genes spatially expressed in regions of the developing kidney involved in branching morphogenesis, nephrogenesis and the development of the collecting duct system, calyces, renal pelvis and ureter. |
| doi_str_mv | 10.1016/j.modgep.2006.02.001 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_68837486</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S1567133X06000299</els_id><sourcerecordid>19298725</sourcerecordid><originalsourceid>FETCH-LOGICAL-c391t-ce18a0ca3f430c842a4c92ff9b0a86fe00f19b50ae17f21fd18a4d14345f2f7f3</originalsourceid><addsrcrecordid>eNqFkEtLAzEQgIMotlb_gcievO2a12aTiyDFFxQ8WMFbSLOTNmVfbrai_96ULXjTy8wcvnl9CF0SnBFMxM02q9tyDV1GMRYZphnG5AhNiSxoWlAlj2OdiyIljL1P0FkI2wgIJdQpmhCR81xQOkXytTODN1WyhgYS-Op6CMG3TeKbZNhAskzDYNaQ1O0uxAiDaaDb9G04RyfOVAEuDnmG3h7ul_OndPHy-Dy_W6SWKTKkFog02BrmOMNWcmq4VdQ5tcJGCgcYO6JWOTZACkeJKyPOS8IZzx11hWMzdD3O7fr2Ywdh0LUPFqoqHhJv0kJKVnAp_gWJilIKmkeQj6CNb4QenO56X5v-WxOs92r1Vo9q9V6txlRHc7Ht6jB_t6qh_G06uIzA7QhA1PHpodfBemgslL4HO-iy9X9v-AFMUIvK</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>19298725</pqid></control><display><type>article</type><title>Spatial gene expression in the T-stage mouse metanephros</title><source>MEDLINE</source><source>ScienceDirect Journals (5 years ago - present)</source><creator>Caruana, Georgina ; Cullen-McEwen, Luise ; Nelson, Amy L. ; Kostoulias, Xenia ; Woods, Kyra ; Gardiner, Brooke ; Davis, Melissa J. ; Taylor, Darrin F. ; Teasdale, Rohan D. ; Grimmond, Sean M. ; Little, Melissa H. ; Bertram, John F.</creator><creatorcontrib>Caruana, Georgina ; Cullen-McEwen, Luise ; Nelson, Amy L. ; Kostoulias, Xenia ; Woods, Kyra ; Gardiner, Brooke ; Davis, Melissa J. ; Taylor, Darrin F. ; Teasdale, Rohan D. ; Grimmond, Sean M. ; Little, Melissa H. ; Bertram, John F.</creatorcontrib><description>The E11.5 mouse metanephros is comprised of a T-stage ureteric epithelial tubule sub-divided into tip and trunk cells surrounded by metanephric mesenchyme (MM). Tip cells are induced to undergo branching morphogenesis by the MM. In contrast, signals within the mesenchyme surrounding the trunk prevent ectopic branching of this region. In order to identify novel genes involved in the molecular regulation of branching morphogenesis we compared the gene expression profiles of isolated tip, trunk and MM cells using Compugen mouse long oligo microarrays. We identified genes enriched in the tip epithelium,
sim-1,
Arg2,
Tacstd1,
Crlf-1 and
BMP7; genes enriched in the trunk epithelium,
Innp1, Itm2b, Mkrn1, SPARC, Emu2 and
Gsta3 and genes spatially restricted to the mesenchyme surrounding the trunk,
CSPG2 and
CV-2, with overlapping and complimentary expression to
BMP4, respectively. This study has identified genes spatially expressed in regions of the developing kidney involved in branching morphogenesis, nephrogenesis and the development of the collecting duct system, calyces, renal pelvis and ureter.</description><identifier>ISSN: 1567-133X</identifier><identifier>EISSN: 1872-7298</identifier><identifier>DOI: 10.1016/j.modgep.2006.02.001</identifier><identifier>PMID: 16545622</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; BMP4 ; Branching morphogenesis ; Crlf-1 ; CSPG2/versican ; CV-2/BMPER ; Embryo, Mammalian - metabolism ; Embryonic Development - physiology ; Epithelial Cells - metabolism ; Gene Expression Profiling - methods ; Gene Expression Regulation, Developmental ; Kidney - embryology ; Kidney - metabolism ; Mesoderm - cytology ; Mesoderm - metabolism ; Metanephric mesenchyme ; Metanephros ; Mice ; Mice, Inbred C57BL ; Mice, Inbred CBA ; Microarray ; Morphogenesis ; sim-1 ; Tacstd1 ; Ureter - cytology ; Ureter - embryology ; Ureter - metabolism ; Ureteric epithelium ; Ureteric tip cells ; Ureteric trunk cells</subject><ispartof>Gene Expression Patterns, 2006-10, Vol.6 (8), p.807-825</ispartof><rights>2006 Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c391t-ce18a0ca3f430c842a4c92ff9b0a86fe00f19b50ae17f21fd18a4d14345f2f7f3</citedby><cites>FETCH-LOGICAL-c391t-ce18a0ca3f430c842a4c92ff9b0a86fe00f19b50ae17f21fd18a4d14345f2f7f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.modgep.2006.02.001$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16545622$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Caruana, Georgina</creatorcontrib><creatorcontrib>Cullen-McEwen, Luise</creatorcontrib><creatorcontrib>Nelson, Amy L.</creatorcontrib><creatorcontrib>Kostoulias, Xenia</creatorcontrib><creatorcontrib>Woods, Kyra</creatorcontrib><creatorcontrib>Gardiner, Brooke</creatorcontrib><creatorcontrib>Davis, Melissa J.</creatorcontrib><creatorcontrib>Taylor, Darrin F.</creatorcontrib><creatorcontrib>Teasdale, Rohan D.</creatorcontrib><creatorcontrib>Grimmond, Sean M.</creatorcontrib><creatorcontrib>Little, Melissa H.</creatorcontrib><creatorcontrib>Bertram, John F.</creatorcontrib><title>Spatial gene expression in the T-stage mouse metanephros</title><title>Gene Expression Patterns</title><addtitle>Gene Expr Patterns</addtitle><description>The E11.5 mouse metanephros is comprised of a T-stage ureteric epithelial tubule sub-divided into tip and trunk cells surrounded by metanephric mesenchyme (MM). Tip cells are induced to undergo branching morphogenesis by the MM. In contrast, signals within the mesenchyme surrounding the trunk prevent ectopic branching of this region. In order to identify novel genes involved in the molecular regulation of branching morphogenesis we compared the gene expression profiles of isolated tip, trunk and MM cells using Compugen mouse long oligo microarrays. We identified genes enriched in the tip epithelium,
sim-1,
Arg2,
Tacstd1,
Crlf-1 and
BMP7; genes enriched in the trunk epithelium,
Innp1, Itm2b, Mkrn1, SPARC, Emu2 and
Gsta3 and genes spatially restricted to the mesenchyme surrounding the trunk,
CSPG2 and
CV-2, with overlapping and complimentary expression to
BMP4, respectively. This study has identified genes spatially expressed in regions of the developing kidney involved in branching morphogenesis, nephrogenesis and the development of the collecting duct system, calyces, renal pelvis and ureter.</description><subject>Animals</subject><subject>BMP4</subject><subject>Branching morphogenesis</subject><subject>Crlf-1</subject><subject>CSPG2/versican</subject><subject>CV-2/BMPER</subject><subject>Embryo, Mammalian - metabolism</subject><subject>Embryonic Development - physiology</subject><subject>Epithelial Cells - metabolism</subject><subject>Gene Expression Profiling - methods</subject><subject>Gene Expression Regulation, Developmental</subject><subject>Kidney - embryology</subject><subject>Kidney - metabolism</subject><subject>Mesoderm - cytology</subject><subject>Mesoderm - metabolism</subject><subject>Metanephric mesenchyme</subject><subject>Metanephros</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Mice, Inbred CBA</subject><subject>Microarray</subject><subject>Morphogenesis</subject><subject>sim-1</subject><subject>Tacstd1</subject><subject>Ureter - cytology</subject><subject>Ureter - embryology</subject><subject>Ureter - metabolism</subject><subject>Ureteric epithelium</subject><subject>Ureteric tip cells</subject><subject>Ureteric trunk cells</subject><issn>1567-133X</issn><issn>1872-7298</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtLAzEQgIMotlb_gcievO2a12aTiyDFFxQ8WMFbSLOTNmVfbrai_96ULXjTy8wcvnl9CF0SnBFMxM02q9tyDV1GMRYZphnG5AhNiSxoWlAlj2OdiyIljL1P0FkI2wgIJdQpmhCR81xQOkXytTODN1WyhgYS-Op6CMG3TeKbZNhAskzDYNaQ1O0uxAiDaaDb9G04RyfOVAEuDnmG3h7ul_OndPHy-Dy_W6SWKTKkFog02BrmOMNWcmq4VdQ5tcJGCgcYO6JWOTZACkeJKyPOS8IZzx11hWMzdD3O7fr2Ywdh0LUPFqoqHhJv0kJKVnAp_gWJilIKmkeQj6CNb4QenO56X5v-WxOs92r1Vo9q9V6txlRHc7Ht6jB_t6qh_G06uIzA7QhA1PHpodfBemgslL4HO-iy9X9v-AFMUIvK</recordid><startdate>20061001</startdate><enddate>20061001</enddate><creator>Caruana, Georgina</creator><creator>Cullen-McEwen, Luise</creator><creator>Nelson, Amy L.</creator><creator>Kostoulias, Xenia</creator><creator>Woods, Kyra</creator><creator>Gardiner, Brooke</creator><creator>Davis, Melissa J.</creator><creator>Taylor, Darrin F.</creator><creator>Teasdale, Rohan D.</creator><creator>Grimmond, Sean M.</creator><creator>Little, Melissa H.</creator><creator>Bertram, John F.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20061001</creationdate><title>Spatial gene expression in the T-stage mouse metanephros</title><author>Caruana, Georgina ; Cullen-McEwen, Luise ; Nelson, Amy L. ; Kostoulias, Xenia ; Woods, Kyra ; Gardiner, Brooke ; Davis, Melissa J. ; Taylor, Darrin F. ; Teasdale, Rohan D. ; Grimmond, Sean M. ; Little, Melissa H. ; Bertram, John F.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c391t-ce18a0ca3f430c842a4c92ff9b0a86fe00f19b50ae17f21fd18a4d14345f2f7f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Animals</topic><topic>BMP4</topic><topic>Branching morphogenesis</topic><topic>Crlf-1</topic><topic>CSPG2/versican</topic><topic>CV-2/BMPER</topic><topic>Embryo, Mammalian - metabolism</topic><topic>Embryonic Development - physiology</topic><topic>Epithelial Cells - metabolism</topic><topic>Gene Expression Profiling - methods</topic><topic>Gene Expression Regulation, Developmental</topic><topic>Kidney - embryology</topic><topic>Kidney - metabolism</topic><topic>Mesoderm - cytology</topic><topic>Mesoderm - metabolism</topic><topic>Metanephric mesenchyme</topic><topic>Metanephros</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Mice, Inbred CBA</topic><topic>Microarray</topic><topic>Morphogenesis</topic><topic>sim-1</topic><topic>Tacstd1</topic><topic>Ureter - cytology</topic><topic>Ureter - embryology</topic><topic>Ureter - metabolism</topic><topic>Ureteric epithelium</topic><topic>Ureteric tip cells</topic><topic>Ureteric trunk cells</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Caruana, Georgina</creatorcontrib><creatorcontrib>Cullen-McEwen, Luise</creatorcontrib><creatorcontrib>Nelson, Amy L.</creatorcontrib><creatorcontrib>Kostoulias, Xenia</creatorcontrib><creatorcontrib>Woods, Kyra</creatorcontrib><creatorcontrib>Gardiner, Brooke</creatorcontrib><creatorcontrib>Davis, Melissa J.</creatorcontrib><creatorcontrib>Taylor, Darrin F.</creatorcontrib><creatorcontrib>Teasdale, Rohan D.</creatorcontrib><creatorcontrib>Grimmond, Sean M.</creatorcontrib><creatorcontrib>Little, Melissa H.</creatorcontrib><creatorcontrib>Bertram, John F.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Gene Expression Patterns</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Caruana, Georgina</au><au>Cullen-McEwen, Luise</au><au>Nelson, Amy L.</au><au>Kostoulias, Xenia</au><au>Woods, Kyra</au><au>Gardiner, Brooke</au><au>Davis, Melissa J.</au><au>Taylor, Darrin F.</au><au>Teasdale, Rohan D.</au><au>Grimmond, Sean M.</au><au>Little, Melissa H.</au><au>Bertram, John F.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Spatial gene expression in the T-stage mouse metanephros</atitle><jtitle>Gene Expression Patterns</jtitle><addtitle>Gene Expr Patterns</addtitle><date>2006-10-01</date><risdate>2006</risdate><volume>6</volume><issue>8</issue><spage>807</spage><epage>825</epage><pages>807-825</pages><issn>1567-133X</issn><eissn>1872-7298</eissn><abstract>The E11.5 mouse metanephros is comprised of a T-stage ureteric epithelial tubule sub-divided into tip and trunk cells surrounded by metanephric mesenchyme (MM). Tip cells are induced to undergo branching morphogenesis by the MM. In contrast, signals within the mesenchyme surrounding the trunk prevent ectopic branching of this region. In order to identify novel genes involved in the molecular regulation of branching morphogenesis we compared the gene expression profiles of isolated tip, trunk and MM cells using Compugen mouse long oligo microarrays. We identified genes enriched in the tip epithelium,
sim-1,
Arg2,
Tacstd1,
Crlf-1 and
BMP7; genes enriched in the trunk epithelium,
Innp1, Itm2b, Mkrn1, SPARC, Emu2 and
Gsta3 and genes spatially restricted to the mesenchyme surrounding the trunk,
CSPG2 and
CV-2, with overlapping and complimentary expression to
BMP4, respectively. This study has identified genes spatially expressed in regions of the developing kidney involved in branching morphogenesis, nephrogenesis and the development of the collecting duct system, calyces, renal pelvis and ureter.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>16545622</pmid><doi>10.1016/j.modgep.2006.02.001</doi><tpages>19</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1567-133X |
| ispartof | Gene Expression Patterns, 2006-10, Vol.6 (8), p.807-825 |
| issn | 1567-133X 1872-7298 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_68837486 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Animals BMP4 Branching morphogenesis Crlf-1 CSPG2/versican CV-2/BMPER Embryo, Mammalian - metabolism Embryonic Development - physiology Epithelial Cells - metabolism Gene Expression Profiling - methods Gene Expression Regulation, Developmental Kidney - embryology Kidney - metabolism Mesoderm - cytology Mesoderm - metabolism Metanephric mesenchyme Metanephros Mice Mice, Inbred C57BL Mice, Inbred CBA Microarray Morphogenesis sim-1 Tacstd1 Ureter - cytology Ureter - embryology Ureter - metabolism Ureteric epithelium Ureteric tip cells Ureteric trunk cells |
| title | Spatial gene expression in the T-stage mouse metanephros |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-07T03%3A35%3A52IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Spatial%20gene%20expression%20in%20the%20T-stage%20mouse%20metanephros&rft.jtitle=Gene%20Expression%20Patterns&rft.au=Caruana,%20Georgina&rft.date=2006-10-01&rft.volume=6&rft.issue=8&rft.spage=807&rft.epage=825&rft.pages=807-825&rft.issn=1567-133X&rft.eissn=1872-7298&rft_id=info:doi/10.1016/j.modgep.2006.02.001&rft_dat=%3Cproquest_cross%3E19298725%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=19298725&rft_id=info:pmid/16545622&rft_els_id=S1567133X06000299&rfr_iscdi=true |