Additional morphological and physiological heterogeneity within the midgut of larval Aedes aegypti (Diptera: Culicidae) revealed by histology, electrophysiology, and effects of Bacillus thuringiensis endotoxin
Analysis of larval Aedes aegypti midgut using scanning electron microscopy, nuclear and mitochondrial dyes, response to Bacillus thuringiensis israelensis CryIVB toxin, and electrophysiology is described. The anterior ventriculus (“stomach”) region is found to have much lower mitochondrial densities...
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| Veröffentlicht in: | Tissue & cell 2005-12, Vol.37 (6), p.457-468 |
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| creator | Clark, T.M. Hutchinson, M.J. Huegel, K.L. Moffett, S.B. Moffett, D.F. |
| description | Analysis of larval
Aedes aegypti midgut using scanning electron microscopy, nuclear and mitochondrial dyes, response to
Bacillus thuringiensis israelensis CryIVB toxin, and electrophysiology is described. The anterior ventriculus (“stomach”) region is found to have much lower mitochondrial densities than other midgut regions. The transitional region is distinguished by apical surface architecture, and by region-specific effects of CryIVB endotoxin. In this region CryIVB causes holes ranging from 1.0 to 7.0
μm in diameter (mean 3.3
±
0.53
μm,
N
=
12), blisters 16.9
±
1.54
μm in diameter (
N
=
10), and separation of adjacent cells. The holes are not consistent with damage due to the colloid osmotic lysis model of δ-endotoxin activity. The posterior ventriculus possesses a distinctive cellular architecture consisting of hemispherical, domed apical membranes surrounded by deep clefts. Functional and morphological heterogeneity is revealed within the posterior ventriculus, with the anterior end dominating the electrical profile of isolated, perfused preparations and showing the greatest response to serotonin. Hyperpolarization of the transepithelial potential by serotonin occurred in conjunction with a decrease in the space constant lambda, ruling out closure of ion channels as the mechanism of action of serotonin. |
| doi_str_mv | 10.1016/j.tice.2005.08.001 |
| format | Article |
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Aedes aegypti midgut using scanning electron microscopy, nuclear and mitochondrial dyes, response to
Bacillus thuringiensis israelensis CryIVB toxin, and electrophysiology is described. The anterior ventriculus (“stomach”) region is found to have much lower mitochondrial densities than other midgut regions. The transitional region is distinguished by apical surface architecture, and by region-specific effects of CryIVB endotoxin. In this region CryIVB causes holes ranging from 1.0 to 7.0
μm in diameter (mean 3.3
±
0.53
μm,
N
=
12), blisters 16.9
±
1.54
μm in diameter (
N
=
10), and separation of adjacent cells. The holes are not consistent with damage due to the colloid osmotic lysis model of δ-endotoxin activity. The posterior ventriculus possesses a distinctive cellular architecture consisting of hemispherical, domed apical membranes surrounded by deep clefts. Functional and morphological heterogeneity is revealed within the posterior ventriculus, with the anterior end dominating the electrical profile of isolated, perfused preparations and showing the greatest response to serotonin. Hyperpolarization of the transepithelial potential by serotonin occurred in conjunction with a decrease in the space constant lambda, ruling out closure of ion channels as the mechanism of action of serotonin.</description><identifier>ISSN: 0040-8166</identifier><identifier>EISSN: 1532-3072</identifier><identifier>DOI: 10.1016/j.tice.2005.08.001</identifier><identifier>PMID: 16221479</identifier><language>eng</language><publisher>Scotland: Elsevier Ltd</publisher><subject>Action Potentials - drug effects ; Aedes - anatomy & histology ; Aedes - cytology ; Aedes - drug effects ; Aedes - physiology ; Aedes aegypti ; Animals ; Bacillus thuringiensis ; Bacillus thuringiensis israelensis ; Bacillus thuringiensis toxin ; Bacterial Proteins - pharmacology ; Bacterial Toxins - pharmacology ; Cell Nucleus - metabolism ; Culicidae ; Digestive System - anatomy & histology ; Diptera ; Electric Conductivity ; Electrophysiology ; Endotoxins - pharmacology ; Hemolysin Proteins ; Histology ; Larva ; Malpighian Tubules - cytology ; Microvilli - ultrastructure ; Midgut morphology ; Mitochondria - metabolism ; Serotonin - pharmacology</subject><ispartof>Tissue & cell, 2005-12, Vol.37 (6), p.457-468</ispartof><rights>2005 Elsevier Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c385t-6cf48b8263bb305d3f50cb02faefe9025837507e3e94f2ce23af7d2ab333442e3</citedby><cites>FETCH-LOGICAL-c385t-6cf48b8263bb305d3f50cb02faefe9025837507e3e94f2ce23af7d2ab333442e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.tice.2005.08.001$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16221479$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Clark, T.M.</creatorcontrib><creatorcontrib>Hutchinson, M.J.</creatorcontrib><creatorcontrib>Huegel, K.L.</creatorcontrib><creatorcontrib>Moffett, S.B.</creatorcontrib><creatorcontrib>Moffett, D.F.</creatorcontrib><title>Additional morphological and physiological heterogeneity within the midgut of larval Aedes aegypti (Diptera: Culicidae) revealed by histology, electrophysiology, and effects of Bacillus thuringiensis endotoxin</title><title>Tissue & cell</title><addtitle>Tissue Cell</addtitle><description>Analysis of larval
Aedes aegypti midgut using scanning electron microscopy, nuclear and mitochondrial dyes, response to
Bacillus thuringiensis israelensis CryIVB toxin, and electrophysiology is described. The anterior ventriculus (“stomach”) region is found to have much lower mitochondrial densities than other midgut regions. The transitional region is distinguished by apical surface architecture, and by region-specific effects of CryIVB endotoxin. In this region CryIVB causes holes ranging from 1.0 to 7.0
μm in diameter (mean 3.3
±
0.53
μm,
N
=
12), blisters 16.9
±
1.54
μm in diameter (
N
=
10), and separation of adjacent cells. The holes are not consistent with damage due to the colloid osmotic lysis model of δ-endotoxin activity. The posterior ventriculus possesses a distinctive cellular architecture consisting of hemispherical, domed apical membranes surrounded by deep clefts. Functional and morphological heterogeneity is revealed within the posterior ventriculus, with the anterior end dominating the electrical profile of isolated, perfused preparations and showing the greatest response to serotonin. Hyperpolarization of the transepithelial potential by serotonin occurred in conjunction with a decrease in the space constant lambda, ruling out closure of ion channels as the mechanism of action of serotonin.</description><subject>Action Potentials - drug effects</subject><subject>Aedes - anatomy & histology</subject><subject>Aedes - cytology</subject><subject>Aedes - drug effects</subject><subject>Aedes - physiology</subject><subject>Aedes aegypti</subject><subject>Animals</subject><subject>Bacillus thuringiensis</subject><subject>Bacillus thuringiensis israelensis</subject><subject>Bacillus thuringiensis toxin</subject><subject>Bacterial Proteins - pharmacology</subject><subject>Bacterial Toxins - pharmacology</subject><subject>Cell Nucleus - metabolism</subject><subject>Culicidae</subject><subject>Digestive System - anatomy & histology</subject><subject>Diptera</subject><subject>Electric Conductivity</subject><subject>Electrophysiology</subject><subject>Endotoxins - pharmacology</subject><subject>Hemolysin Proteins</subject><subject>Histology</subject><subject>Larva</subject><subject>Malpighian Tubules - cytology</subject><subject>Microvilli - ultrastructure</subject><subject>Midgut morphology</subject><subject>Mitochondria - metabolism</subject><subject>Serotonin - pharmacology</subject><issn>0040-8166</issn><issn>1532-3072</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUcuO0zAUjRCIGQZ-gAXyCoFEix95FbEp5SmNxAbWlmNfN7dy42A7hXwmf4SjVsMOVpaPzuPqnKJ4yuiaUVa_PqwTalhzSqs1bdeUsnvFNasEXwna8PvFNaUlXbWsrq-KRzEeKKVNyZqHxRWrOWdls7kufm-NwYR-UI4cfRh77_wedf6pwZCxnyPeIT0kCH4PA2CayU9MPQ4k9UCOaPZTIt4Sp8IpM7dgIBIF-3lMSF68xzEr1RuymxxqNApekgAnUA4M6WbSY0xLyvyKgAOdgr8LztByCFib8bhEvFManZtiTp4CDnuEIWIkMBif_C8cHhcPrHIRnlzem-L7xw_fdp9Xt18_fdltb1datFVa1dqWbdfyWnSdoJURtqK6o9wqsLChvGpFU9EGBGxKyzVwoWxjuOqEEGXJQdwUz8--Y_A_JohJHjFqcE4N4Kco67YVbJPn-B-RbdqyKusmE_mZqIOPMYCVY8CjCrNkVC6Ly4NcFpfL4pK2Mi-eRc8u7lN3BPNXcpk4E96eCZDLOCEEGXXuTIPBkDuVxuO__P8AVCfDFQ</recordid><startdate>20051201</startdate><enddate>20051201</enddate><creator>Clark, T.M.</creator><creator>Hutchinson, M.J.</creator><creator>Huegel, K.L.</creator><creator>Moffett, S.B.</creator><creator>Moffett, D.F.</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7SS</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>F1W</scope><scope>FR3</scope><scope>H95</scope><scope>L.G</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20051201</creationdate><title>Additional morphological and physiological heterogeneity within the midgut of larval Aedes aegypti (Diptera: Culicidae) revealed by histology, electrophysiology, and effects of Bacillus thuringiensis endotoxin</title><author>Clark, T.M. ; Hutchinson, M.J. ; Huegel, K.L. ; Moffett, S.B. ; Moffett, D.F.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c385t-6cf48b8263bb305d3f50cb02faefe9025837507e3e94f2ce23af7d2ab333442e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Action Potentials - drug effects</topic><topic>Aedes - anatomy & histology</topic><topic>Aedes - cytology</topic><topic>Aedes - drug effects</topic><topic>Aedes - physiology</topic><topic>Aedes aegypti</topic><topic>Animals</topic><topic>Bacillus thuringiensis</topic><topic>Bacillus thuringiensis israelensis</topic><topic>Bacillus thuringiensis toxin</topic><topic>Bacterial Proteins - pharmacology</topic><topic>Bacterial Toxins - pharmacology</topic><topic>Cell Nucleus - metabolism</topic><topic>Culicidae</topic><topic>Digestive System - anatomy & histology</topic><topic>Diptera</topic><topic>Electric Conductivity</topic><topic>Electrophysiology</topic><topic>Endotoxins - pharmacology</topic><topic>Hemolysin Proteins</topic><topic>Histology</topic><topic>Larva</topic><topic>Malpighian Tubules - cytology</topic><topic>Microvilli - ultrastructure</topic><topic>Midgut morphology</topic><topic>Mitochondria - metabolism</topic><topic>Serotonin - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Clark, T.M.</creatorcontrib><creatorcontrib>Hutchinson, M.J.</creatorcontrib><creatorcontrib>Huegel, K.L.</creatorcontrib><creatorcontrib>Moffett, S.B.</creatorcontrib><creatorcontrib>Moffett, D.F.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Tissue & cell</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Clark, T.M.</au><au>Hutchinson, M.J.</au><au>Huegel, K.L.</au><au>Moffett, S.B.</au><au>Moffett, D.F.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Additional morphological and physiological heterogeneity within the midgut of larval Aedes aegypti (Diptera: Culicidae) revealed by histology, electrophysiology, and effects of Bacillus thuringiensis endotoxin</atitle><jtitle>Tissue & cell</jtitle><addtitle>Tissue Cell</addtitle><date>2005-12-01</date><risdate>2005</risdate><volume>37</volume><issue>6</issue><spage>457</spage><epage>468</epage><pages>457-468</pages><issn>0040-8166</issn><eissn>1532-3072</eissn><abstract>Analysis of larval
Aedes aegypti midgut using scanning electron microscopy, nuclear and mitochondrial dyes, response to
Bacillus thuringiensis israelensis CryIVB toxin, and electrophysiology is described. The anterior ventriculus (“stomach”) region is found to have much lower mitochondrial densities than other midgut regions. The transitional region is distinguished by apical surface architecture, and by region-specific effects of CryIVB endotoxin. In this region CryIVB causes holes ranging from 1.0 to 7.0
μm in diameter (mean 3.3
±
0.53
μm,
N
=
12), blisters 16.9
±
1.54
μm in diameter (
N
=
10), and separation of adjacent cells. The holes are not consistent with damage due to the colloid osmotic lysis model of δ-endotoxin activity. The posterior ventriculus possesses a distinctive cellular architecture consisting of hemispherical, domed apical membranes surrounded by deep clefts. Functional and morphological heterogeneity is revealed within the posterior ventriculus, with the anterior end dominating the electrical profile of isolated, perfused preparations and showing the greatest response to serotonin. Hyperpolarization of the transepithelial potential by serotonin occurred in conjunction with a decrease in the space constant lambda, ruling out closure of ion channels as the mechanism of action of serotonin.</abstract><cop>Scotland</cop><pub>Elsevier Ltd</pub><pmid>16221479</pmid><doi>10.1016/j.tice.2005.08.001</doi><tpages>12</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0040-8166 |
| ispartof | Tissue & cell, 2005-12, Vol.37 (6), p.457-468 |
| issn | 0040-8166 1532-3072 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_68831915 |
| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Action Potentials - drug effects Aedes - anatomy & histology Aedes - cytology Aedes - drug effects Aedes - physiology Aedes aegypti Animals Bacillus thuringiensis Bacillus thuringiensis israelensis Bacillus thuringiensis toxin Bacterial Proteins - pharmacology Bacterial Toxins - pharmacology Cell Nucleus - metabolism Culicidae Digestive System - anatomy & histology Diptera Electric Conductivity Electrophysiology Endotoxins - pharmacology Hemolysin Proteins Histology Larva Malpighian Tubules - cytology Microvilli - ultrastructure Midgut morphology Mitochondria - metabolism Serotonin - pharmacology |
| title | Additional morphological and physiological heterogeneity within the midgut of larval Aedes aegypti (Diptera: Culicidae) revealed by histology, electrophysiology, and effects of Bacillus thuringiensis endotoxin |
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