Identification of Mytilus spp. and Pecten maximus in Irish waters by standard PCR of the 18S rDNA gene and multiplex PCR of the 16S rDNA gene
Two molecular protocols for the identification of mussel and scallop have been developed using specific primers targeting the mitochondrial 16S ribosomal DNA gene and the nuclear 18S ribosomal DNA gene. Primers for the mitochondrial 16S ribosomal DNA gene in multiplex polymerase chain reaction (PCR)...
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| Veröffentlicht in: | Marine biotechnology (New York, N.Y.) N.Y.), 2005-12, Vol.7 (6), p.687-696 |
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| description | Two molecular protocols for the identification of mussel and scallop have been developed using specific primers targeting the mitochondrial 16S ribosomal DNA gene and the nuclear 18S ribosomal DNA gene. Primers for the mitochondrial 16S ribosomal DNA gene in multiplex polymerase chain reaction (PCR) protocols yielded diagnostic DNA fragments for the mussels Mytilus edulis, Mytilus galloprovincialis, and the hybrid Mytilus edulis/galloprovincialis (335 bp), the king scallop Pecten maximus (382 bp) and the black scallop Mimachlamys varia (398 bp). DNA from the queen scallop Aequipecten opercularis showed no consistent PCR amplification of the 16S rDNA gene. Primers for the nuclear 18S rDNA gene in standard PCR protocols yielded similar-sized, diagnostic DNA fragments (approx. 190 bp) for the mussels Mytilus edulis, Mytilus galloprovincialis, and the hybrid Mytilus edulis/galloprovincialis, the king scallop Pecten maximus, the black scallop Mimachlamys varia, and the queen scallop Aequipecten opercularis. Both protocols have been tested with Mytilus spp., P. maximus, and 6 other bivalve species from a wide range of locations in Irish and European waters. Cross reaction of the specific primers with DNA template from any of the 6 other bivalve species was not observed. Rapid DNA extraction using FTA Card technology and the16S rDNA primers allowed for the detection of at least 10 mussel larvae in a subsample of natural plankton. |
| doi_str_mv | 10.1007/s10126-004-0124-y |
| format | Article |
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Primers for the mitochondrial 16S ribosomal DNA gene in multiplex polymerase chain reaction (PCR) protocols yielded diagnostic DNA fragments for the mussels Mytilus edulis, Mytilus galloprovincialis, and the hybrid Mytilus edulis/galloprovincialis (335 bp), the king scallop Pecten maximus (382 bp) and the black scallop Mimachlamys varia (398 bp). DNA from the queen scallop Aequipecten opercularis showed no consistent PCR amplification of the 16S rDNA gene. Primers for the nuclear 18S rDNA gene in standard PCR protocols yielded similar-sized, diagnostic DNA fragments (approx. 190 bp) for the mussels Mytilus edulis, Mytilus galloprovincialis, and the hybrid Mytilus edulis/galloprovincialis, the king scallop Pecten maximus, the black scallop Mimachlamys varia, and the queen scallop Aequipecten opercularis. Both protocols have been tested with Mytilus spp., P. maximus, and 6 other bivalve species from a wide range of locations in Irish and European waters. Cross reaction of the specific primers with DNA template from any of the 6 other bivalve species was not observed. Rapid DNA extraction using FTA Card technology and the16S rDNA primers allowed for the detection of at least 10 mussel larvae in a subsample of natural plankton.</description><identifier>ISSN: 1436-2228</identifier><identifier>EISSN: 1436-2236</identifier><identifier>DOI: 10.1007/s10126-004-0124-y</identifier><identifier>PMID: 16206017</identifier><language>eng</language><publisher>United States: Springer Nature B.V</publisher><subject>Aequipecten opercularis ; Animals ; Bacteria ; Bivalvia ; DNA Primers ; DNA, Ribosomal - genetics ; Ireland ; Larvae ; Marine ; Microbiology ; Mimachlamys varia ; Mollusks ; Mytilus - genetics ; Mytilus edulis ; Mytilus galloprovincialis ; Pecten - genetics ; Pecten maximus ; Physical growth ; Plankton ; Polymerase Chain Reaction ; Ribosomal DNA ; Species Specificity</subject><ispartof>Marine biotechnology (New York, N.Y.), 2005-12, Vol.7 (6), p.687-696</ispartof><rights>Springer Science+Business Media, Inc. 2005</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c386t-4bf1ced793ff93d96e0762e3e6673e69dc202d418a0bfb7ef6bc1ef0af116a323</citedby><cites>FETCH-LOGICAL-c386t-4bf1ced793ff93d96e0762e3e6673e69dc202d418a0bfb7ef6bc1ef0af116a323</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,27926,27927</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16206017$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bendezu, Ivan F</creatorcontrib><creatorcontrib>Slater, John W</creatorcontrib><creatorcontrib>Carney, Brian F</creatorcontrib><title>Identification of Mytilus spp. and Pecten maximus in Irish waters by standard PCR of the 18S rDNA gene and multiplex PCR of the 16S rDNA gene</title><title>Marine biotechnology (New York, N.Y.)</title><addtitle>Mar Biotechnol (NY)</addtitle><description>Two molecular protocols for the identification of mussel and scallop have been developed using specific primers targeting the mitochondrial 16S ribosomal DNA gene and the nuclear 18S ribosomal DNA gene. Primers for the mitochondrial 16S ribosomal DNA gene in multiplex polymerase chain reaction (PCR) protocols yielded diagnostic DNA fragments for the mussels Mytilus edulis, Mytilus galloprovincialis, and the hybrid Mytilus edulis/galloprovincialis (335 bp), the king scallop Pecten maximus (382 bp) and the black scallop Mimachlamys varia (398 bp). DNA from the queen scallop Aequipecten opercularis showed no consistent PCR amplification of the 16S rDNA gene. Primers for the nuclear 18S rDNA gene in standard PCR protocols yielded similar-sized, diagnostic DNA fragments (approx. 190 bp) for the mussels Mytilus edulis, Mytilus galloprovincialis, and the hybrid Mytilus edulis/galloprovincialis, the king scallop Pecten maximus, the black scallop Mimachlamys varia, and the queen scallop Aequipecten opercularis. Both protocols have been tested with Mytilus spp., P. maximus, and 6 other bivalve species from a wide range of locations in Irish and European waters. Cross reaction of the specific primers with DNA template from any of the 6 other bivalve species was not observed. Rapid DNA extraction using FTA Card technology and the16S rDNA primers allowed for the detection of at least 10 mussel larvae in a subsample of natural plankton.</description><subject>Aequipecten opercularis</subject><subject>Animals</subject><subject>Bacteria</subject><subject>Bivalvia</subject><subject>DNA Primers</subject><subject>DNA, Ribosomal - genetics</subject><subject>Ireland</subject><subject>Larvae</subject><subject>Marine</subject><subject>Microbiology</subject><subject>Mimachlamys varia</subject><subject>Mollusks</subject><subject>Mytilus - genetics</subject><subject>Mytilus edulis</subject><subject>Mytilus galloprovincialis</subject><subject>Pecten - genetics</subject><subject>Pecten maximus</subject><subject>Physical growth</subject><subject>Plankton</subject><subject>Polymerase Chain Reaction</subject><subject>Ribosomal DNA</subject><subject>Species 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Primers for the mitochondrial 16S ribosomal DNA gene in multiplex polymerase chain reaction (PCR) protocols yielded diagnostic DNA fragments for the mussels Mytilus edulis, Mytilus galloprovincialis, and the hybrid Mytilus edulis/galloprovincialis (335 bp), the king scallop Pecten maximus (382 bp) and the black scallop Mimachlamys varia (398 bp). DNA from the queen scallop Aequipecten opercularis showed no consistent PCR amplification of the 16S rDNA gene. Primers for the nuclear 18S rDNA gene in standard PCR protocols yielded similar-sized, diagnostic DNA fragments (approx. 190 bp) for the mussels Mytilus edulis, Mytilus galloprovincialis, and the hybrid Mytilus edulis/galloprovincialis, the king scallop Pecten maximus, the black scallop Mimachlamys varia, and the queen scallop Aequipecten opercularis. Both protocols have been tested with Mytilus spp., P. maximus, and 6 other bivalve species from a wide range of locations in Irish and European waters. Cross reaction of the specific primers with DNA template from any of the 6 other bivalve species was not observed. Rapid DNA extraction using FTA Card technology and the16S rDNA primers allowed for the detection of at least 10 mussel larvae in a subsample of natural plankton.</abstract><cop>United States</cop><pub>Springer Nature B.V</pub><pmid>16206017</pmid><doi>10.1007/s10126-004-0124-y</doi><tpages>10</tpages></addata></record> |
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| ispartof | Marine biotechnology (New York, N.Y.), 2005-12, Vol.7 (6), p.687-696 |
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| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Aequipecten opercularis Animals Bacteria Bivalvia DNA Primers DNA, Ribosomal - genetics Ireland Larvae Marine Microbiology Mimachlamys varia Mollusks Mytilus - genetics Mytilus edulis Mytilus galloprovincialis Pecten - genetics Pecten maximus Physical growth Plankton Polymerase Chain Reaction Ribosomal DNA Species Specificity |
| title | Identification of Mytilus spp. and Pecten maximus in Irish waters by standard PCR of the 18S rDNA gene and multiplex PCR of the 16S rDNA gene |
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