Simple pulse-acquire NMR methods for the quantitative analysis of calcium, magnesium and sodium in human serum
Simple pulse-acquire NMR methods are presented for accurate quantitation of calcium (Ca 2+), magnesium (Mg 2+) and sodium (Na +) in human serum. Ca 2+ and Mg 2+ can be determined simultaneously by 1H NMR via their EDTA (ethylenediaminetetraacetic acid) complexes. Spectra are acquired before and afte...
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| Veröffentlicht in: | Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy Molecular and biomolecular spectroscopy, 2006-10, Vol.65 (2), p.254-260 |
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| container_title | Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy |
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| creator | Somashekar, B.S. Ijare, Omkar B. Nagana Gowda, G.A. Ramesh, V. Gupta, Sushil Khetrapal, C.L. |
| description | Simple pulse-acquire NMR methods are presented for accurate quantitation of calcium (Ca
2+), magnesium (Mg
2+) and sodium (Na
+) in human serum. Ca
2+ and Mg
2+ can be determined simultaneously by
1H NMR via their EDTA (ethylenediaminetetraacetic acid) complexes. Spectra are acquired before and after addition of EDTA, and the difference spectrum is used for integration of the signals from the complexes relative to an internal reference. Serum sodium can also be determined through pulse-acquire
23Na NMR by integration of the free sodium signal relative to a reference signal (Na
+
+
EDTA in a coaxial capillary tube). The method shows excellent accuracy and precision for all three metal ions. Slow chemical exchange between complexed and free EDTA at the natural pH of serum does not limit the accuracy of the determination of Ca
2+ and Mg
2+, and the large errors associated with spin-echo spectral editing methods are avoided. |
| doi_str_mv | 10.1016/j.saa.2005.10.039 |
| format | Article |
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2+), magnesium (Mg
2+) and sodium (Na
+) in human serum. Ca
2+ and Mg
2+ can be determined simultaneously by
1H NMR via their EDTA (ethylenediaminetetraacetic acid) complexes. Spectra are acquired before and after addition of EDTA, and the difference spectrum is used for integration of the signals from the complexes relative to an internal reference. Serum sodium can also be determined through pulse-acquire
23Na NMR by integration of the free sodium signal relative to a reference signal (Na
+
+
EDTA in a coaxial capillary tube). The method shows excellent accuracy and precision for all three metal ions. Slow chemical exchange between complexed and free EDTA at the natural pH of serum does not limit the accuracy of the determination of Ca
2+ and Mg
2+, and the large errors associated with spin-echo spectral editing methods are avoided.</description><identifier>ISSN: 1386-1425</identifier><identifier>DOI: 10.1016/j.saa.2005.10.039</identifier><identifier>PMID: 16490390</identifier><language>eng</language><publisher>England: Elsevier B.V</publisher><subject>Calcium ; Calcium - blood ; Edetic Acid ; Humans ; Hydrogen-Ion Concentration ; Magnesium ; Magnesium - blood ; NMR ; Nuclear Magnetic Resonance, Biomolecular - methods ; Quantitation ; Reference Values ; Reproducibility of Results ; Serum ; Sodium ; Sodium - blood</subject><ispartof>Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, 2006-10, Vol.65 (2), p.254-260</ispartof><rights>2005 Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c351t-12f8f8f24b7cc7336dd2cba88ee7e5e77f6d62efb542516109061ba0f260fc063</citedby><cites>FETCH-LOGICAL-c351t-12f8f8f24b7cc7336dd2cba88ee7e5e77f6d62efb542516109061ba0f260fc063</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.saa.2005.10.039$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16490390$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Somashekar, B.S.</creatorcontrib><creatorcontrib>Ijare, Omkar B.</creatorcontrib><creatorcontrib>Nagana Gowda, G.A.</creatorcontrib><creatorcontrib>Ramesh, V.</creatorcontrib><creatorcontrib>Gupta, Sushil</creatorcontrib><creatorcontrib>Khetrapal, C.L.</creatorcontrib><title>Simple pulse-acquire NMR methods for the quantitative analysis of calcium, magnesium and sodium in human serum</title><title>Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy</title><addtitle>Spectrochim Acta A Mol Biomol Spectrosc</addtitle><description>Simple pulse-acquire NMR methods are presented for accurate quantitation of calcium (Ca
2+), magnesium (Mg
2+) and sodium (Na
+) in human serum. Ca
2+ and Mg
2+ can be determined simultaneously by
1H NMR via their EDTA (ethylenediaminetetraacetic acid) complexes. Spectra are acquired before and after addition of EDTA, and the difference spectrum is used for integration of the signals from the complexes relative to an internal reference. Serum sodium can also be determined through pulse-acquire
23Na NMR by integration of the free sodium signal relative to a reference signal (Na
+
+
EDTA in a coaxial capillary tube). The method shows excellent accuracy and precision for all three metal ions. Slow chemical exchange between complexed and free EDTA at the natural pH of serum does not limit the accuracy of the determination of Ca
2+ and Mg
2+, and the large errors associated with spin-echo spectral editing methods are avoided.</description><subject>Calcium</subject><subject>Calcium - blood</subject><subject>Edetic Acid</subject><subject>Humans</subject><subject>Hydrogen-Ion Concentration</subject><subject>Magnesium</subject><subject>Magnesium - blood</subject><subject>NMR</subject><subject>Nuclear Magnetic Resonance, Biomolecular - methods</subject><subject>Quantitation</subject><subject>Reference Values</subject><subject>Reproducibility of Results</subject><subject>Serum</subject><subject>Sodium</subject><subject>Sodium - blood</subject><issn>1386-1425</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMtOAyEUhllobK0-gBvDypVTgekw07gyjbfES-JlTRg4WJphpoWhSd9eJm3izrDgAN_5yfkQuqBkSgnlN6tpkHLKCCnSeUry-REa07ziGZ2xYoROQ1gRQmjFyAkaUT6bJ4SMUftp3boBvI5NgEyqTbQe8NvrB3bQLzsdsOk87peAN1G2ve1lb7eAZSubXbABdwYr2Sgb3TV28qeFkMr0rHHo9FDaFi-jky0O4KM7Q8dGpp_OD_sEfT_cfy2espf3x-fF3Uum8oL2GWWmSovN6lKpMs-51kzVsqoASiigLA3XnIGpizQc5ZTMCae1JIZxYhTh-QRd7XPXvttECL1wNihoGtlCF4PgVcV4Uc0SSPeg8l0IHoxYe-uk3wlKxCBWrEQSKwaxw1WylnouD-GxdqD_Og5WE3C7ByCNuLXgRVAWWgU6yVW90J39J_4X8XuMWg</recordid><startdate>20061001</startdate><enddate>20061001</enddate><creator>Somashekar, B.S.</creator><creator>Ijare, Omkar B.</creator><creator>Nagana Gowda, G.A.</creator><creator>Ramesh, V.</creator><creator>Gupta, Sushil</creator><creator>Khetrapal, C.L.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20061001</creationdate><title>Simple pulse-acquire NMR methods for the quantitative analysis of calcium, magnesium and sodium in human serum</title><author>Somashekar, B.S. ; Ijare, Omkar B. ; Nagana Gowda, G.A. ; Ramesh, V. ; Gupta, Sushil ; Khetrapal, C.L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c351t-12f8f8f24b7cc7336dd2cba88ee7e5e77f6d62efb542516109061ba0f260fc063</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Calcium</topic><topic>Calcium - blood</topic><topic>Edetic Acid</topic><topic>Humans</topic><topic>Hydrogen-Ion Concentration</topic><topic>Magnesium</topic><topic>Magnesium - blood</topic><topic>NMR</topic><topic>Nuclear Magnetic Resonance, Biomolecular - methods</topic><topic>Quantitation</topic><topic>Reference Values</topic><topic>Reproducibility of Results</topic><topic>Serum</topic><topic>Sodium</topic><topic>Sodium - blood</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Somashekar, B.S.</creatorcontrib><creatorcontrib>Ijare, Omkar B.</creatorcontrib><creatorcontrib>Nagana Gowda, G.A.</creatorcontrib><creatorcontrib>Ramesh, V.</creatorcontrib><creatorcontrib>Gupta, Sushil</creatorcontrib><creatorcontrib>Khetrapal, C.L.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Somashekar, B.S.</au><au>Ijare, Omkar B.</au><au>Nagana Gowda, G.A.</au><au>Ramesh, V.</au><au>Gupta, Sushil</au><au>Khetrapal, C.L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Simple pulse-acquire NMR methods for the quantitative analysis of calcium, magnesium and sodium in human serum</atitle><jtitle>Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy</jtitle><addtitle>Spectrochim Acta A Mol Biomol Spectrosc</addtitle><date>2006-10-01</date><risdate>2006</risdate><volume>65</volume><issue>2</issue><spage>254</spage><epage>260</epage><pages>254-260</pages><issn>1386-1425</issn><abstract>Simple pulse-acquire NMR methods are presented for accurate quantitation of calcium (Ca
2+), magnesium (Mg
2+) and sodium (Na
+) in human serum. Ca
2+ and Mg
2+ can be determined simultaneously by
1H NMR via their EDTA (ethylenediaminetetraacetic acid) complexes. Spectra are acquired before and after addition of EDTA, and the difference spectrum is used for integration of the signals from the complexes relative to an internal reference. Serum sodium can also be determined through pulse-acquire
23Na NMR by integration of the free sodium signal relative to a reference signal (Na
+
+
EDTA in a coaxial capillary tube). The method shows excellent accuracy and precision for all three metal ions. Slow chemical exchange between complexed and free EDTA at the natural pH of serum does not limit the accuracy of the determination of Ca
2+ and Mg
2+, and the large errors associated with spin-echo spectral editing methods are avoided.</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>16490390</pmid><doi>10.1016/j.saa.2005.10.039</doi><tpages>7</tpages></addata></record> |
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| identifier | ISSN: 1386-1425 |
| ispartof | Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, 2006-10, Vol.65 (2), p.254-260 |
| issn | 1386-1425 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_68826584 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Calcium Calcium - blood Edetic Acid Humans Hydrogen-Ion Concentration Magnesium Magnesium - blood NMR Nuclear Magnetic Resonance, Biomolecular - methods Quantitation Reference Values Reproducibility of Results Serum Sodium Sodium - blood |
| title | Simple pulse-acquire NMR methods for the quantitative analysis of calcium, magnesium and sodium in human serum |
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