Identification of Salmonella spp. isolates from chicken abattoirs by multiplex-PCR
The present study was carried out to report the occurrence Salmonella spp., Salmonella Enteritidis, and Salmonella Typhimurium in chicken abattoirs. Samples of feces; feathers; scald, evisceration, and chiller water; and rinse water of non-eviscerated, eviscerated, and chilled carcass were collected...
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| Veröffentlicht in: | Research in veterinary science 2006-12, Vol.81 (3), p.340-344 |
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| creator | Cortez, A.L.L. Carvalho, A.C.F.B. Ikuno, A.A. Bürger, K.P. Vidal-Martins, A.M.C. |
| description | The present study was carried out to report the occurrence
Salmonella spp.,
Salmonella Enteritidis, and
Salmonella Typhimurium in chicken abattoirs. Samples of feces; feathers; scald, evisceration, and chiller water; and rinse water of non-eviscerated, eviscerated, and chilled carcass were collected from six chicken abattoirs.
Salmonella isolates were identified by a multiplex-PCR using three sets of primers targeting the
invA,
pefA, and
sefA gene sequences from
Salmonella spp.,
S. Typhimurium and
S. Enteritidis, respectively.
Salmonella spp. was detected in 10% (29/288) of the samples, whereas serovars Enteritidis and Typhimurium were identified in 62% (7/288), respectively. The results indicate the need to improve hygiene and sanitary standards in poultry slaughter lines, besides the education of food handlers and information to consumers. |
| doi_str_mv | 10.1016/j.rvsc.2006.03.006 |
| format | Article |
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Salmonella spp.,
Salmonella Enteritidis, and
Salmonella Typhimurium in chicken abattoirs. Samples of feces; feathers; scald, evisceration, and chiller water; and rinse water of non-eviscerated, eviscerated, and chilled carcass were collected from six chicken abattoirs.
Salmonella isolates were identified by a multiplex-PCR using three sets of primers targeting the
invA,
pefA, and
sefA gene sequences from
Salmonella spp.,
S. Typhimurium and
S. Enteritidis, respectively.
Salmonella spp. was detected in 10% (29/288) of the samples, whereas serovars Enteritidis and Typhimurium were identified in 62% (7/288), respectively. The results indicate the need to improve hygiene and sanitary standards in poultry slaughter lines, besides the education of food handlers and information to consumers.</description><identifier>ISSN: 0034-5288</identifier><identifier>EISSN: 1532-2661</identifier><identifier>DOI: 10.1016/j.rvsc.2006.03.006</identifier><identifier>PMID: 16701750</identifier><language>eng</language><publisher>England: Elsevier India Pvt Ltd</publisher><subject>Abattoirs - standards ; Animals ; Bacteria ; bacterial contamination ; Chicken abattoir ; chickens ; Chickens - microbiology ; Eggs ; Feces ; Food contamination & poisoning ; Methods ; Microbiology ; Multiplex-PCR ; multiplex-polymerase chain reaction ; pathogen identification ; polymerase chain reaction ; Polymerase Chain Reaction - methods ; Polymerase Chain Reaction - veterinary ; Poultry ; Salmonella ; Salmonella - isolation & purification ; Salmonella Enteritidis ; Salmonella spp ; Salmonella Typhimurium ; slaughterhouses ; Veterinary medicine</subject><ispartof>Research in veterinary science, 2006-12, Vol.81 (3), p.340-344</ispartof><rights>2006 Elsevier Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c406t-cd4459fc669febe8c8364eaa19dbe14338f944e6a3419f6aa02819340b6ec563</citedby><cites>FETCH-LOGICAL-c406t-cd4459fc669febe8c8364eaa19dbe14338f944e6a3419f6aa02819340b6ec563</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0034528806000786$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16701750$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cortez, A.L.L.</creatorcontrib><creatorcontrib>Carvalho, A.C.F.B.</creatorcontrib><creatorcontrib>Ikuno, A.A.</creatorcontrib><creatorcontrib>Bürger, K.P.</creatorcontrib><creatorcontrib>Vidal-Martins, A.M.C.</creatorcontrib><title>Identification of Salmonella spp. isolates from chicken abattoirs by multiplex-PCR</title><title>Research in veterinary science</title><addtitle>Res Vet Sci</addtitle><description>The present study was carried out to report the occurrence
Salmonella spp.,
Salmonella Enteritidis, and
Salmonella Typhimurium in chicken abattoirs. Samples of feces; feathers; scald, evisceration, and chiller water; and rinse water of non-eviscerated, eviscerated, and chilled carcass were collected from six chicken abattoirs.
Salmonella isolates were identified by a multiplex-PCR using three sets of primers targeting the
invA,
pefA, and
sefA gene sequences from
Salmonella spp.,
S. Typhimurium and
S. Enteritidis, respectively.
Salmonella spp. was detected in 10% (29/288) of the samples, whereas serovars Enteritidis and Typhimurium were identified in 62% (7/288), respectively. The results indicate the need to improve hygiene and sanitary standards in poultry slaughter lines, besides the education of food handlers and information to consumers.</description><subject>Abattoirs - standards</subject><subject>Animals</subject><subject>Bacteria</subject><subject>bacterial contamination</subject><subject>Chicken abattoir</subject><subject>chickens</subject><subject>Chickens - microbiology</subject><subject>Eggs</subject><subject>Feces</subject><subject>Food contamination & poisoning</subject><subject>Methods</subject><subject>Microbiology</subject><subject>Multiplex-PCR</subject><subject>multiplex-polymerase chain reaction</subject><subject>pathogen identification</subject><subject>polymerase chain reaction</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Polymerase Chain Reaction - veterinary</subject><subject>Poultry</subject><subject>Salmonella</subject><subject>Salmonella - isolation & purification</subject><subject>Salmonella Enteritidis</subject><subject>Salmonella spp</subject><subject>Salmonella Typhimurium</subject><subject>slaughterhouses</subject><subject>Veterinary medicine</subject><issn>0034-5288</issn><issn>1532-2661</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE2L1TAYhYMozp3RP-BCA4K71jcfTVtwM1x0HBhQZsZ1SNM3mmvb1CQdnH9vy70guHB1Ns85HB5CXjEoGTD1_lDGh2RLDqBKEOUaT8iOVYIXXCn2lOwAhCwq3jRn5DylAwBIxurn5IypGlhdwY7cXvc4Ze-8NdmHiQZH78wwhgmHwdA0zyX1KQwmY6IuhpHaH97-xImazuQcfEy0e6TjMmQ_D_i7-Lq_fUGeOTMkfHnKC3L_6eP9_nNx8-Xqen95U1gJKhe2l7JqnVWqddhhYxuhJBrD2r5DJoVoXCslKiMka50yBnjDWiGhU2grJS7Iu-PsHMOvBVPWo092uz1hWJJWTcMrBnwF3_4DHsISp_WaZiCq1VErN4ofKRtDShGdnqMfTXxcIb3p1ge96dabbg1Cr7GWXp-ml27E_m_l5HcF3hwBZ4I236NP-tsdByYAalHVfCM-HAlcVT14jDpZj5PF3ke0WffB_-_BH1y2mZg</recordid><startdate>20061201</startdate><enddate>20061201</enddate><creator>Cortez, A.L.L.</creator><creator>Carvalho, A.C.F.B.</creator><creator>Ikuno, A.A.</creator><creator>Bürger, K.P.</creator><creator>Vidal-Martins, A.M.C.</creator><general>Elsevier India Pvt Ltd</general><general>Elsevier Limited</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QP</scope><scope>7QR</scope><scope>7T5</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20061201</creationdate><title>Identification of Salmonella spp. isolates from chicken abattoirs by multiplex-PCR</title><author>Cortez, A.L.L. ; Carvalho, A.C.F.B. ; Ikuno, A.A. ; Bürger, K.P. ; Vidal-Martins, A.M.C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c406t-cd4459fc669febe8c8364eaa19dbe14338f944e6a3419f6aa02819340b6ec563</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Abattoirs - standards</topic><topic>Animals</topic><topic>Bacteria</topic><topic>bacterial contamination</topic><topic>Chicken abattoir</topic><topic>chickens</topic><topic>Chickens - microbiology</topic><topic>Eggs</topic><topic>Feces</topic><topic>Food contamination & poisoning</topic><topic>Methods</topic><topic>Microbiology</topic><topic>Multiplex-PCR</topic><topic>multiplex-polymerase chain reaction</topic><topic>pathogen identification</topic><topic>polymerase chain reaction</topic><topic>Polymerase Chain Reaction - methods</topic><topic>Polymerase Chain Reaction - veterinary</topic><topic>Poultry</topic><topic>Salmonella</topic><topic>Salmonella - isolation & purification</topic><topic>Salmonella Enteritidis</topic><topic>Salmonella spp</topic><topic>Salmonella Typhimurium</topic><topic>slaughterhouses</topic><topic>Veterinary medicine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cortez, A.L.L.</creatorcontrib><creatorcontrib>Carvalho, A.C.F.B.</creatorcontrib><creatorcontrib>Ikuno, A.A.</creatorcontrib><creatorcontrib>Bürger, K.P.</creatorcontrib><creatorcontrib>Vidal-Martins, A.M.C.</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Research in veterinary science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cortez, A.L.L.</au><au>Carvalho, A.C.F.B.</au><au>Ikuno, A.A.</au><au>Bürger, K.P.</au><au>Vidal-Martins, A.M.C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of Salmonella spp. isolates from chicken abattoirs by multiplex-PCR</atitle><jtitle>Research in veterinary science</jtitle><addtitle>Res Vet Sci</addtitle><date>2006-12-01</date><risdate>2006</risdate><volume>81</volume><issue>3</issue><spage>340</spage><epage>344</epage><pages>340-344</pages><issn>0034-5288</issn><eissn>1532-2661</eissn><abstract>The present study was carried out to report the occurrence
Salmonella spp.,
Salmonella Enteritidis, and
Salmonella Typhimurium in chicken abattoirs. Samples of feces; feathers; scald, evisceration, and chiller water; and rinse water of non-eviscerated, eviscerated, and chilled carcass were collected from six chicken abattoirs.
Salmonella isolates were identified by a multiplex-PCR using three sets of primers targeting the
invA,
pefA, and
sefA gene sequences from
Salmonella spp.,
S. Typhimurium and
S. Enteritidis, respectively.
Salmonella spp. was detected in 10% (29/288) of the samples, whereas serovars Enteritidis and Typhimurium were identified in 62% (7/288), respectively. The results indicate the need to improve hygiene and sanitary standards in poultry slaughter lines, besides the education of food handlers and information to consumers.</abstract><cop>England</cop><pub>Elsevier India Pvt Ltd</pub><pmid>16701750</pmid><doi>10.1016/j.rvsc.2006.03.006</doi><tpages>5</tpages></addata></record> |
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| ispartof | Research in veterinary science, 2006-12, Vol.81 (3), p.340-344 |
| issn | 0034-5288 1532-2661 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Abattoirs - standards Animals Bacteria bacterial contamination Chicken abattoir chickens Chickens - microbiology Eggs Feces Food contamination & poisoning Methods Microbiology Multiplex-PCR multiplex-polymerase chain reaction pathogen identification polymerase chain reaction Polymerase Chain Reaction - methods Polymerase Chain Reaction - veterinary Poultry Salmonella Salmonella - isolation & purification Salmonella Enteritidis Salmonella spp Salmonella Typhimurium slaughterhouses Veterinary medicine |
| title | Identification of Salmonella spp. isolates from chicken abattoirs by multiplex-PCR |
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