The effect of methyl jasmonate on triterpene and sterol metabolisms of Centella asiatica, Ruscus aculeatus and Galphimia glauca cultured plants
In this work we have studied the effect of treatment with the elicitor methyl jasmonate on the biosynthesis of bioactive terpenoids and free sterols of C. asiatica, R. aculeatus and G. glauca plantlets. Considering that exogenously applied methyl jasmonate can enhance secondary metabolite production...
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| Veröffentlicht in: | Phytochemistry (Oxford) 2006-09, Vol.67 (18), p.2041-2049 |
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| creator | Mangas, Susana Bonfill, Mercè Osuna, Lidia Moyano, Elisabeth Tortoriello, Jaime Cusido, Rosa M. Teresa Piñol, M. Palazón, Javier |
| description | In this work we have studied the effect of treatment with the elicitor methyl jasmonate on the biosynthesis of bioactive terpenoids and free sterols of
C. asiatica,
R. aculeatus and
G. glauca plantlets.
Considering that exogenously applied methyl jasmonate can enhance secondary metabolite production in a variety of plant species and that 2,3-oxidosqualene is a common precursor of triterpenes and sterols in plants, we have studied
Centella asiatica and
Galphimia glauca (both synthesizing triterpenoid secondary compounds) and
Ruscus aculeatus (which synthesizes steroidal secondary compounds) for their growth rate and content of free sterols and respective secondary compounds, after culturing with or without 100
μM methyl jasmonate. Our results show that elicited plantlets of
G. glauca and to a higher degree
C. asiatica (up to 152-times more) increased their content of triterpenoids directly synthesized from 2,3-oxidosqualene (ursane saponins and nor-seco-friedelane galphimines, respectively) at the same time as growth decreased. In contrast, the free sterol content of
C. asiatica decreased notably, and remained practically unaltered in
G. glauca. However, in the case of
R. aculeatus, which synthesizes steroidal saponins (mainly spirostane type) indirectly from 2,3-oxidosqualene after the latter is converted to the plant phytosterol-precursor cycloartenol, while the growth rate and free sterol content clearly decreased, the spirostane saponine content was virtually unchanged (aerial part) or somewhat lower (roots) in presence of the same elicitor concentration. Our results suggest that while methyl jasmonate may be used as an inducer of enzymes involved in the triterpenoid synthesis downstream from 2,3-oxidosqualene in both
C. asiatica and
G. glauca plantlets, in those of
C. asiatica and
R. aculeatus it inhibited the enzymes involved in sterol synthesis downstream from cycloartenol. |
| doi_str_mv | 10.1016/j.phytochem.2006.06.025 |
| format | Article |
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C. asiatica,
R. aculeatus and
G. glauca plantlets.
Considering that exogenously applied methyl jasmonate can enhance secondary metabolite production in a variety of plant species and that 2,3-oxidosqualene is a common precursor of triterpenes and sterols in plants, we have studied
Centella asiatica and
Galphimia glauca (both synthesizing triterpenoid secondary compounds) and
Ruscus aculeatus (which synthesizes steroidal secondary compounds) for their growth rate and content of free sterols and respective secondary compounds, after culturing with or without 100
μM methyl jasmonate. Our results show that elicited plantlets of
G. glauca and to a higher degree
C. asiatica (up to 152-times more) increased their content of triterpenoids directly synthesized from 2,3-oxidosqualene (ursane saponins and nor-seco-friedelane galphimines, respectively) at the same time as growth decreased. In contrast, the free sterol content of
C. asiatica decreased notably, and remained practically unaltered in
G. glauca. However, in the case of
R. aculeatus, which synthesizes steroidal saponins (mainly spirostane type) indirectly from 2,3-oxidosqualene after the latter is converted to the plant phytosterol-precursor cycloartenol, while the growth rate and free sterol content clearly decreased, the spirostane saponine content was virtually unchanged (aerial part) or somewhat lower (roots) in presence of the same elicitor concentration. Our results suggest that while methyl jasmonate may be used as an inducer of enzymes involved in the triterpenoid synthesis downstream from 2,3-oxidosqualene in both
C. asiatica and
G. glauca plantlets, in those of
C. asiatica and
R. aculeatus it inhibited the enzymes involved in sterol synthesis downstream from cycloartenol.</description><identifier>ISSN: 0031-9422</identifier><identifier>EISSN: 1873-3700</identifier><identifier>DOI: 10.1016/j.phytochem.2006.06.025</identifier><identifier>PMID: 16876832</identifier><language>eng</language><publisher>Amsterdam: Elsevier Ltd</publisher><subject>Acetates - pharmacology ; Biological and medical sciences ; Centella - chemistry ; Centella - drug effects ; Centella - metabolism ; Centella asiatica ; centelloside ; Chemical constitution ; Cyclopentanes - pharmacology ; Elicitation ; Fundamental and applied biological sciences. Psychology ; Galphimia ; Galphimia - drug effects ; Galphimia - metabolism ; Galphimia glauca ; Galphimine-B ; herbaceous plants ; medicinal plants ; Metabolism ; Metabolism. Physicochemical requirements ; methyl jasmonate ; Molecular Structure ; Oxylipins ; Phytosterols ; plant biochemistry ; plant growth ; Plant Growth Regulators - pharmacology ; Plant physiology and development ; Ruscus - drug effects ; Ruscus - metabolism ; Ruscus aculeatus ; secondary metabolites ; shrubs ; Spirostane saponins ; Sterols - chemistry ; Sterols - metabolism ; terpenoids ; Triterpenes ; Triterpenes - chemistry ; Triterpenes - metabolism ; triterpenoids ; Ursane saponins</subject><ispartof>Phytochemistry (Oxford), 2006-09, Vol.67 (18), p.2041-2049</ispartof><rights>2006 Elsevier Ltd</rights><rights>2006 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c489t-69663b288109178b198e3cc2751d0788c88c4efcaa6b559854b3c212b70808053</citedby><cites>FETCH-LOGICAL-c489t-69663b288109178b198e3cc2751d0788c88c4efcaa6b559854b3c212b70808053</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.phytochem.2006.06.025$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,781,785,3551,27929,27930,46000</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18110265$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16876832$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mangas, Susana</creatorcontrib><creatorcontrib>Bonfill, Mercè</creatorcontrib><creatorcontrib>Osuna, Lidia</creatorcontrib><creatorcontrib>Moyano, Elisabeth</creatorcontrib><creatorcontrib>Tortoriello, Jaime</creatorcontrib><creatorcontrib>Cusido, Rosa M.</creatorcontrib><creatorcontrib>Teresa Piñol, M.</creatorcontrib><creatorcontrib>Palazón, Javier</creatorcontrib><title>The effect of methyl jasmonate on triterpene and sterol metabolisms of Centella asiatica, Ruscus aculeatus and Galphimia glauca cultured plants</title><title>Phytochemistry (Oxford)</title><addtitle>Phytochemistry</addtitle><description>In this work we have studied the effect of treatment with the elicitor methyl jasmonate on the biosynthesis of bioactive terpenoids and free sterols of
C. asiatica,
R. aculeatus and
G. glauca plantlets.
Considering that exogenously applied methyl jasmonate can enhance secondary metabolite production in a variety of plant species and that 2,3-oxidosqualene is a common precursor of triterpenes and sterols in plants, we have studied
Centella asiatica and
Galphimia glauca (both synthesizing triterpenoid secondary compounds) and
Ruscus aculeatus (which synthesizes steroidal secondary compounds) for their growth rate and content of free sterols and respective secondary compounds, after culturing with or without 100
μM methyl jasmonate. Our results show that elicited plantlets of
G. glauca and to a higher degree
C. asiatica (up to 152-times more) increased their content of triterpenoids directly synthesized from 2,3-oxidosqualene (ursane saponins and nor-seco-friedelane galphimines, respectively) at the same time as growth decreased. In contrast, the free sterol content of
C. asiatica decreased notably, and remained practically unaltered in
G. glauca. However, in the case of
R. aculeatus, which synthesizes steroidal saponins (mainly spirostane type) indirectly from 2,3-oxidosqualene after the latter is converted to the plant phytosterol-precursor cycloartenol, while the growth rate and free sterol content clearly decreased, the spirostane saponine content was virtually unchanged (aerial part) or somewhat lower (roots) in presence of the same elicitor concentration. Our results suggest that while methyl jasmonate may be used as an inducer of enzymes involved in the triterpenoid synthesis downstream from 2,3-oxidosqualene in both
C. asiatica and
G. glauca plantlets, in those of
C. asiatica and
R. aculeatus it inhibited the enzymes involved in sterol synthesis downstream from cycloartenol.</description><subject>Acetates - pharmacology</subject><subject>Biological and medical sciences</subject><subject>Centella - chemistry</subject><subject>Centella - drug effects</subject><subject>Centella - metabolism</subject><subject>Centella asiatica</subject><subject>centelloside</subject><subject>Chemical constitution</subject><subject>Cyclopentanes - pharmacology</subject><subject>Elicitation</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Galphimia</subject><subject>Galphimia - drug effects</subject><subject>Galphimia - metabolism</subject><subject>Galphimia glauca</subject><subject>Galphimine-B</subject><subject>herbaceous plants</subject><subject>medicinal plants</subject><subject>Metabolism</subject><subject>Metabolism. Physicochemical requirements</subject><subject>methyl jasmonate</subject><subject>Molecular Structure</subject><subject>Oxylipins</subject><subject>Phytosterols</subject><subject>plant biochemistry</subject><subject>plant growth</subject><subject>Plant Growth Regulators - pharmacology</subject><subject>Plant physiology and development</subject><subject>Ruscus - drug effects</subject><subject>Ruscus - metabolism</subject><subject>Ruscus aculeatus</subject><subject>secondary metabolites</subject><subject>shrubs</subject><subject>Spirostane saponins</subject><subject>Sterols - chemistry</subject><subject>Sterols - metabolism</subject><subject>terpenoids</subject><subject>Triterpenes</subject><subject>Triterpenes - chemistry</subject><subject>Triterpenes - metabolism</subject><subject>triterpenoids</subject><subject>Ursane saponins</subject><issn>0031-9422</issn><issn>1873-3700</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcFu1DAQhiMEoqXwCtQXOLHL2Ekc51itoCBVQoL2bE2cSdcrJw62U2mfglfG0a7oEXkkj-Xvn7HnL4prDlsOXH4-bOf9MXmzp3ErAOR2DVG_KC65aspN2QC8LC4BSr5pKyEuijcxHgCgrqV8XVxwqRqpSnFZ_LnfE6NhIJOYH9hIaX907IBx9BMmYn5iKdhEYaaJGE49i_ng3Upi552NY1yFO5oSOYcMo8VkDX5iP5dolsjQLI4wrVlW36Kb93a0yB4dLgZZvk1LoJ7NDqcU3xavBnSR3p33q-Lh65f73bfN3Y_b77ubu42pVJs2spWy7IRSHFreqI63ikpjRFPzHhqlTI6KBoMou7puVV11pRFcdA2ovOryqvh4qjsH_3uhmPRoo1l_MJFfopZKiQqqNoPNCTTBxxho0HOwI4aj5qBXL_RB__NCr17oNcTa4v25xdKN1D_rzsPPwIczgNGgGwJOxsZnTnEOQq6Frk_cgF7jY8jMwy8BvIQMAIcyEzcngvLIniwFHY2lyVBvQ3ZW997-97l_AfWXtwY</recordid><startdate>20060901</startdate><enddate>20060901</enddate><creator>Mangas, Susana</creator><creator>Bonfill, Mercè</creator><creator>Osuna, Lidia</creator><creator>Moyano, Elisabeth</creator><creator>Tortoriello, Jaime</creator><creator>Cusido, Rosa M.</creator><creator>Teresa Piñol, M.</creator><creator>Palazón, Javier</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20060901</creationdate><title>The effect of methyl jasmonate on triterpene and sterol metabolisms of Centella asiatica, Ruscus aculeatus and Galphimia glauca cultured plants</title><author>Mangas, Susana ; Bonfill, Mercè ; Osuna, Lidia ; Moyano, Elisabeth ; Tortoriello, Jaime ; Cusido, Rosa M. ; Teresa Piñol, M. ; Palazón, Javier</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c489t-69663b288109178b198e3cc2751d0788c88c4efcaa6b559854b3c212b70808053</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Acetates - pharmacology</topic><topic>Biological and medical sciences</topic><topic>Centella - chemistry</topic><topic>Centella - drug effects</topic><topic>Centella - metabolism</topic><topic>Centella asiatica</topic><topic>centelloside</topic><topic>Chemical constitution</topic><topic>Cyclopentanes - pharmacology</topic><topic>Elicitation</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Galphimia</topic><topic>Galphimia - drug effects</topic><topic>Galphimia - metabolism</topic><topic>Galphimia glauca</topic><topic>Galphimine-B</topic><topic>herbaceous plants</topic><topic>medicinal plants</topic><topic>Metabolism</topic><topic>Metabolism. Physicochemical requirements</topic><topic>methyl jasmonate</topic><topic>Molecular Structure</topic><topic>Oxylipins</topic><topic>Phytosterols</topic><topic>plant biochemistry</topic><topic>plant growth</topic><topic>Plant Growth Regulators - pharmacology</topic><topic>Plant physiology and development</topic><topic>Ruscus - drug effects</topic><topic>Ruscus - metabolism</topic><topic>Ruscus aculeatus</topic><topic>secondary metabolites</topic><topic>shrubs</topic><topic>Spirostane saponins</topic><topic>Sterols - chemistry</topic><topic>Sterols - metabolism</topic><topic>terpenoids</topic><topic>Triterpenes</topic><topic>Triterpenes - chemistry</topic><topic>Triterpenes - metabolism</topic><topic>triterpenoids</topic><topic>Ursane saponins</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mangas, Susana</creatorcontrib><creatorcontrib>Bonfill, Mercè</creatorcontrib><creatorcontrib>Osuna, Lidia</creatorcontrib><creatorcontrib>Moyano, Elisabeth</creatorcontrib><creatorcontrib>Tortoriello, Jaime</creatorcontrib><creatorcontrib>Cusido, Rosa M.</creatorcontrib><creatorcontrib>Teresa Piñol, M.</creatorcontrib><creatorcontrib>Palazón, Javier</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Phytochemistry (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mangas, Susana</au><au>Bonfill, Mercè</au><au>Osuna, Lidia</au><au>Moyano, Elisabeth</au><au>Tortoriello, Jaime</au><au>Cusido, Rosa M.</au><au>Teresa Piñol, M.</au><au>Palazón, Javier</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The effect of methyl jasmonate on triterpene and sterol metabolisms of Centella asiatica, Ruscus aculeatus and Galphimia glauca cultured plants</atitle><jtitle>Phytochemistry (Oxford)</jtitle><addtitle>Phytochemistry</addtitle><date>2006-09-01</date><risdate>2006</risdate><volume>67</volume><issue>18</issue><spage>2041</spage><epage>2049</epage><pages>2041-2049</pages><issn>0031-9422</issn><eissn>1873-3700</eissn><abstract>In this work we have studied the effect of treatment with the elicitor methyl jasmonate on the biosynthesis of bioactive terpenoids and free sterols of
C. asiatica,
R. aculeatus and
G. glauca plantlets.
Considering that exogenously applied methyl jasmonate can enhance secondary metabolite production in a variety of plant species and that 2,3-oxidosqualene is a common precursor of triterpenes and sterols in plants, we have studied
Centella asiatica and
Galphimia glauca (both synthesizing triterpenoid secondary compounds) and
Ruscus aculeatus (which synthesizes steroidal secondary compounds) for their growth rate and content of free sterols and respective secondary compounds, after culturing with or without 100
μM methyl jasmonate. Our results show that elicited plantlets of
G. glauca and to a higher degree
C. asiatica (up to 152-times more) increased their content of triterpenoids directly synthesized from 2,3-oxidosqualene (ursane saponins and nor-seco-friedelane galphimines, respectively) at the same time as growth decreased. In contrast, the free sterol content of
C. asiatica decreased notably, and remained practically unaltered in
G. glauca. However, in the case of
R. aculeatus, which synthesizes steroidal saponins (mainly spirostane type) indirectly from 2,3-oxidosqualene after the latter is converted to the plant phytosterol-precursor cycloartenol, while the growth rate and free sterol content clearly decreased, the spirostane saponine content was virtually unchanged (aerial part) or somewhat lower (roots) in presence of the same elicitor concentration. Our results suggest that while methyl jasmonate may be used as an inducer of enzymes involved in the triterpenoid synthesis downstream from 2,3-oxidosqualene in both
C. asiatica and
G. glauca plantlets, in those of
C. asiatica and
R. aculeatus it inhibited the enzymes involved in sterol synthesis downstream from cycloartenol.</abstract><cop>Amsterdam</cop><pub>Elsevier Ltd</pub><pmid>16876832</pmid><doi>10.1016/j.phytochem.2006.06.025</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0031-9422 |
| ispartof | Phytochemistry (Oxford), 2006-09, Vol.67 (18), p.2041-2049 |
| issn | 0031-9422 1873-3700 |
| language | eng |
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| subjects | Acetates - pharmacology Biological and medical sciences Centella - chemistry Centella - drug effects Centella - metabolism Centella asiatica centelloside Chemical constitution Cyclopentanes - pharmacology Elicitation Fundamental and applied biological sciences. Psychology Galphimia Galphimia - drug effects Galphimia - metabolism Galphimia glauca Galphimine-B herbaceous plants medicinal plants Metabolism Metabolism. Physicochemical requirements methyl jasmonate Molecular Structure Oxylipins Phytosterols plant biochemistry plant growth Plant Growth Regulators - pharmacology Plant physiology and development Ruscus - drug effects Ruscus - metabolism Ruscus aculeatus secondary metabolites shrubs Spirostane saponins Sterols - chemistry Sterols - metabolism terpenoids Triterpenes Triterpenes - chemistry Triterpenes - metabolism triterpenoids Ursane saponins |
| title | The effect of methyl jasmonate on triterpene and sterol metabolisms of Centella asiatica, Ruscus aculeatus and Galphimia glauca cultured plants |
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