In vitro regeneration and Agrobacterium-mediated genetic transformation of Euonymus alatus

An in vitro plant regeneration method and an Agrobacterium tumefaciens-mediated genetic transformation protocol were developed for Euonymus alatus. More than 60% of cotyledon and 70% of hypocotyl sections from 10-day-old seedlings of E. alatus produced 2-4 shoots on woody plant medium (WPM) suppleme...

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Veröffentlicht in:	Plant cell reports 2006-10, Vol.25 (10), p.1043-1051
Hauptverfasser:	Chen, Y, Lu, L, Deng, W, Yang, X, McAvoy, R, Zhao, D, Pei, Y, Luo, K, Duan, H, Smith, W
Format:	Artikel
Sprache:	eng
Schlagworte:	Acetic acid Agrobacterium tumefaciens Agrobacterium tumefaciens - metabolism Bacteria benzyladenine beta-glucuronidase Biological and medical sciences Biotechnology Castor Cotyledon - physiology cotyledons culture media DNA, Bacterial - metabolism Eukaryotic cell cultures Euonymus - genetics Euonymus - physiology Euonymus alatus Fundamental and applied biological sciences. Psychology Genes genetic transformation genetic vectors Genome, Plant - genetics Hypocotyl - physiology hypocotyls Methods. Procedures. Technologies micropropagation Miscellaneous Naphthalene naphthaleneacetic acid ornamental plants Plant cells and fungal cells Plant Leaves - cytology plant regeneration Plant Roots - cytology plantlets Plants, Genetically Modified Regeneration Ricinus communis Seedlings Shoots Transformation, Genetic transgenes transgenic plants Woody plants
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