Increased nicotianamine biosynthesis confers enhanced tolerance of high levels of metals, in particular nickel, to plants
Nicotianamine, a plant-derived chelator of metals, is produced by the trimerization of S-adenosylmethionine catalyzed by nicotianamine synthase. We established transgenic Arabidopsis and tobacco plants that constitutively overexpress the barley nicotianamine synthase gene. Nicotianamine synthase ove...
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| Veröffentlicht in: | Plant and cell physiology 2005-11, Vol.46 (11), p.1809-1818 |
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| container_issue | 11 |
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| container_title | Plant and cell physiology |
| container_volume | 46 |
| creator | Kim, S.(Tokyo Univ. (Japan)) Takahashi, M Higuchi, K Tsunoda, K Nakanishi, H Yoshimura, E Mori, S Nishizawa, N.K |
| description | Nicotianamine, a plant-derived chelator of metals, is produced by the trimerization of S-adenosylmethionine catalyzed by nicotianamine synthase. We established transgenic Arabidopsis and tobacco plants that constitutively overexpress the barley nicotianamine synthase gene. Nicotianamine synthase overexpression resulted in increased biosynthesis of nicotianamine in transgenic plants, which conferred enhanced tolerance of high levels of metals, particularly nickel, to plants. Promoter activities of four nicotianamine synthase genes in Arabidopsis were all increased in response to excess nickel, suggesting that nicotianamine plays an important role in the detoxification of nickel in plants. Furthermore, transgenic tobacco plants with a high level of nicotianamine grew well in a nickel-enriched serpentine soil without developing any symptoms of nickel toxicity. Our results indicate that nicotianamine plays a critical role in metal detoxification, and this can be a powerful tool for use in phytoremediation. |
| doi_str_mv | 10.1093/pcp/pci196 |
| format | Article |
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(Japan)) ; Takahashi, M ; Higuchi, K ; Tsunoda, K ; Nakanishi, H ; Yoshimura, E ; Mori, S ; Nishizawa, N.K</creator><creatorcontrib>Kim, S.(Tokyo Univ. (Japan)) ; Takahashi, M ; Higuchi, K ; Tsunoda, K ; Nakanishi, H ; Yoshimura, E ; Mori, S ; Nishizawa, N.K</creatorcontrib><description>Nicotianamine, a plant-derived chelator of metals, is produced by the trimerization of S-adenosylmethionine catalyzed by nicotianamine synthase. We established transgenic Arabidopsis and tobacco plants that constitutively overexpress the barley nicotianamine synthase gene. Nicotianamine synthase overexpression resulted in increased biosynthesis of nicotianamine in transgenic plants, which conferred enhanced tolerance of high levels of metals, particularly nickel, to plants. Promoter activities of four nicotianamine synthase genes in Arabidopsis were all increased in response to excess nickel, suggesting that nicotianamine plays an important role in the detoxification of nickel in plants. Furthermore, transgenic tobacco plants with a high level of nicotianamine grew well in a nickel-enriched serpentine soil without developing any symptoms of nickel toxicity. Our results indicate that nicotianamine plays a critical role in metal detoxification, and this can be a powerful tool for use in phytoremediation.</description><identifier>ISSN: 0032-0781</identifier><identifier>EISSN: 1471-9053</identifier><identifier>DOI: 10.1093/pcp/pci196</identifier><identifier>PMID: 16143596</identifier><language>eng</language><publisher>Japan: Oxford University Press</publisher><subject>Adaptation, Physiological ; Alkyl and Aryl Transferases - genetics ; Alkyl and Aryl Transferases - metabolism ; ARABIDOPSIS ; Arabidopsis - drug effects ; Arabidopsis - genetics ; Arabidopsis - metabolism ; Arabidopsis - physiology ; Azetidinecarboxylic Acid - analogs & derivatives ; Azetidinecarboxylic Acid - metabolism ; Base Sequence ; BIODECONTAMINACION ; BIOREMEDIATION ; Blotting, Northern ; CaMV ; cauliflower mosaic virus ; DNA Primers ; Genes, Plant ; GUS ; Hordeum vulgare ; Metal toxicity ; Metals - pharmacology ; NAAT ; NAS ; NICKEL ; Nicotiana - drug effects ; Nicotiana - metabolism ; Nicotiana - physiology ; nicotianamine ; nicotianamine aminotransferase ; Nicotianamine synthase ; NIQUEL ; non-transgenic ; open reading frame ; ORF ; Phytoremediation ; Promoter Regions, Genetic ; Tobacco ; TOXICIDAD ; TOXICITE ; TOXICITY ; β-glucuronidase</subject><ispartof>Plant and cell physiology, 2005-11, Vol.46 (11), p.1809-1818</ispartof><rights>Copyright Oxford University Press(England) Nov 2005</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c530t-ee938065ee4c7eaf0149ddaf8c188761ae580eead6dc3a86f4b2b5950b20fa993</citedby><cites>FETCH-LOGICAL-c530t-ee938065ee4c7eaf0149ddaf8c188761ae580eead6dc3a86f4b2b5950b20fa993</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16143596$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kim, S.(Tokyo Univ. (Japan))</creatorcontrib><creatorcontrib>Takahashi, M</creatorcontrib><creatorcontrib>Higuchi, K</creatorcontrib><creatorcontrib>Tsunoda, K</creatorcontrib><creatorcontrib>Nakanishi, H</creatorcontrib><creatorcontrib>Yoshimura, E</creatorcontrib><creatorcontrib>Mori, S</creatorcontrib><creatorcontrib>Nishizawa, N.K</creatorcontrib><title>Increased nicotianamine biosynthesis confers enhanced tolerance of high levels of metals, in particular nickel, to plants</title><title>Plant and cell physiology</title><addtitle>Plant Cell Physiol</addtitle><description>Nicotianamine, a plant-derived chelator of metals, is produced by the trimerization of S-adenosylmethionine catalyzed by nicotianamine synthase. We established transgenic Arabidopsis and tobacco plants that constitutively overexpress the barley nicotianamine synthase gene. Nicotianamine synthase overexpression resulted in increased biosynthesis of nicotianamine in transgenic plants, which conferred enhanced tolerance of high levels of metals, particularly nickel, to plants. Promoter activities of four nicotianamine synthase genes in Arabidopsis were all increased in response to excess nickel, suggesting that nicotianamine plays an important role in the detoxification of nickel in plants. Furthermore, transgenic tobacco plants with a high level of nicotianamine grew well in a nickel-enriched serpentine soil without developing any symptoms of nickel toxicity. Our results indicate that nicotianamine plays a critical role in metal detoxification, and this can be a powerful tool for use in phytoremediation.</description><subject>Adaptation, Physiological</subject><subject>Alkyl and Aryl Transferases - genetics</subject><subject>Alkyl and Aryl Transferases - metabolism</subject><subject>ARABIDOPSIS</subject><subject>Arabidopsis - drug effects</subject><subject>Arabidopsis - genetics</subject><subject>Arabidopsis - metabolism</subject><subject>Arabidopsis - physiology</subject><subject>Azetidinecarboxylic Acid - analogs & derivatives</subject><subject>Azetidinecarboxylic Acid - metabolism</subject><subject>Base Sequence</subject><subject>BIODECONTAMINACION</subject><subject>BIOREMEDIATION</subject><subject>Blotting, Northern</subject><subject>CaMV</subject><subject>cauliflower mosaic virus</subject><subject>DNA Primers</subject><subject>Genes, Plant</subject><subject>GUS</subject><subject>Hordeum vulgare</subject><subject>Metal toxicity</subject><subject>Metals - pharmacology</subject><subject>NAAT</subject><subject>NAS</subject><subject>NICKEL</subject><subject>Nicotiana - drug effects</subject><subject>Nicotiana - metabolism</subject><subject>Nicotiana - physiology</subject><subject>nicotianamine</subject><subject>nicotianamine aminotransferase</subject><subject>Nicotianamine synthase</subject><subject>NIQUEL</subject><subject>non-transgenic</subject><subject>open reading frame</subject><subject>ORF</subject><subject>Phytoremediation</subject><subject>Promoter Regions, Genetic</subject><subject>Tobacco</subject><subject>TOXICIDAD</subject><subject>TOXICITE</subject><subject>TOXICITY</subject><subject>β-glucuronidase</subject><issn>0032-0781</issn><issn>1471-9053</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0UGL1DAUB_AgijuuXrwrwYMH2epL06TJUXbVWV1QQUG8hDR93clum9akFefbm6GDghcPIS_kl8cLf0IeM3jJQPNXk5vy8kzLO2TDqpoVGgS_SzYAvCygVuyEPEjpBiDXHO6TEyZZxYWWG7K_DC6iTdjS4N04exvs4APSxo9pH-YdJp-oG0OHMVEMOxtctvPYYzyUdOzozl_vaI8_sU-H44Cz7dMZ9YFONs7eLb2Nh-632J_ll3TqbZjTQ3Kvyw4fHfdT8vXtmy_n2-Lq47vL89dXhRMc5gJRcwVSIFauRtsBq3Tb2k45plQtmUWhANG2snXcKtlVTdkILaApobNa81PyfO07xfHHgmk2g08O-zwEjksyUqmyrKX8LyxBCaVkmeGzf-DNuMSQP5FNHk9qXmX0YkUujilF7MwU_WDj3jAwh9hMjs2ssWX89NhxaQZs_9JjThkUK_Bpxl9_7m28NbLmtTDbb9_Nxeet-iDEhamzf7L6zo7GXkefzPtPJYAEqBTn_DevF62i</recordid><startdate>200511</startdate><enddate>200511</enddate><creator>Kim, S.(Tokyo Univ. 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(Japan))</au><au>Takahashi, M</au><au>Higuchi, K</au><au>Tsunoda, K</au><au>Nakanishi, H</au><au>Yoshimura, E</au><au>Mori, S</au><au>Nishizawa, N.K</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Increased nicotianamine biosynthesis confers enhanced tolerance of high levels of metals, in particular nickel, to plants</atitle><jtitle>Plant and cell physiology</jtitle><addtitle>Plant Cell Physiol</addtitle><date>2005-11</date><risdate>2005</risdate><volume>46</volume><issue>11</issue><spage>1809</spage><epage>1818</epage><pages>1809-1818</pages><issn>0032-0781</issn><eissn>1471-9053</eissn><abstract>Nicotianamine, a plant-derived chelator of metals, is produced by the trimerization of S-adenosylmethionine catalyzed by nicotianamine synthase. We established transgenic Arabidopsis and tobacco plants that constitutively overexpress the barley nicotianamine synthase gene. Nicotianamine synthase overexpression resulted in increased biosynthesis of nicotianamine in transgenic plants, which conferred enhanced tolerance of high levels of metals, particularly nickel, to plants. Promoter activities of four nicotianamine synthase genes in Arabidopsis were all increased in response to excess nickel, suggesting that nicotianamine plays an important role in the detoxification of nickel in plants. Furthermore, transgenic tobacco plants with a high level of nicotianamine grew well in a nickel-enriched serpentine soil without developing any symptoms of nickel toxicity. Our results indicate that nicotianamine plays a critical role in metal detoxification, and this can be a powerful tool for use in phytoremediation.</abstract><cop>Japan</cop><pub>Oxford University Press</pub><pmid>16143596</pmid><doi>10.1093/pcp/pci196</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Adaptation, Physiological Alkyl and Aryl Transferases - genetics Alkyl and Aryl Transferases - metabolism ARABIDOPSIS Arabidopsis - drug effects Arabidopsis - genetics Arabidopsis - metabolism Arabidopsis - physiology Azetidinecarboxylic Acid - analogs & derivatives Azetidinecarboxylic Acid - metabolism Base Sequence BIODECONTAMINACION BIOREMEDIATION Blotting, Northern CaMV cauliflower mosaic virus DNA Primers Genes, Plant GUS Hordeum vulgare Metal toxicity Metals - pharmacology NAAT NAS NICKEL Nicotiana - drug effects Nicotiana - metabolism Nicotiana - physiology nicotianamine nicotianamine aminotransferase Nicotianamine synthase NIQUEL non-transgenic open reading frame ORF Phytoremediation Promoter Regions, Genetic Tobacco TOXICIDAD TOXICITE TOXICITY β-glucuronidase |
| title | Increased nicotianamine biosynthesis confers enhanced tolerance of high levels of metals, in particular nickel, to plants |
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