Cytokine-induced prostaglandin E2 production and cyclooxygenase-2 expression in dental pulp cells: downstream calcium signalling via activation of prostaglandin EP receptor
Aim To determine whether (i) proinflammatory cytokines stimulate prostaglandin E2 (PGE2) production and cyclooxygenase (COX) gene expression in dental pulp cells, and (ii) pulp cells that express different prostaglandin E2 receptor (EP) isoforms and their activation by PGE2 leads to downstream Ca2+...
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| Veröffentlicht in: | International endodontic journal 2006-10, Vol.39 (10), p.819-826 |
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| creator | Chang, M.-C. Chen, Y.-J. Tai, T.-F. Tai, M.-R. Li, M.-Y. Tsai, Y.-L. Lan, W.-H. Wang, Y.-L. Jeng, J.-H. |
| description | Aim To determine whether (i) proinflammatory cytokines stimulate prostaglandin E2 (PGE2) production and cyclooxygenase (COX) gene expression in dental pulp cells, and (ii) pulp cells that express different prostaglandin E2 receptor (EP) isoforms and their activation by PGE2 leads to downstream Ca2+ signalling.
Methodology Cultured human dental pulp cells were exposed to interleukin (IL)‐1β and tumour necrotic factor‐alpha (TNF‐α). The expression of COX‐1 and COX‐2 was measured with reverse transcriptase‐polymerase chain reaction (RT‐PCR). The production of PGE2 was measured using an enzyme‐linked immunosorbent assay. Expression of prostaglandin EP receptor isoforms was studied by RT‐PCR, whereas fura‐2 fluorescence was used to measure calcium mobilization. The Kruskal–Wallis test and Wilcoxon sum rank test with Bonferroni correction were used for statistical analysis.
Results Interleukin‐1β and TNF‐α stimulate PGE2 production of human dental pulp cells (P |
| doi_str_mv | 10.1111/j.1365-2591.2006.01156.x |
| format | Article |
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Methodology Cultured human dental pulp cells were exposed to interleukin (IL)‐1β and tumour necrotic factor‐alpha (TNF‐α). The expression of COX‐1 and COX‐2 was measured with reverse transcriptase‐polymerase chain reaction (RT‐PCR). The production of PGE2 was measured using an enzyme‐linked immunosorbent assay. Expression of prostaglandin EP receptor isoforms was studied by RT‐PCR, whereas fura‐2 fluorescence was used to measure calcium mobilization. The Kruskal–Wallis test and Wilcoxon sum rank test with Bonferroni correction were used for statistical analysis.
Results Interleukin‐1β and TNF‐α stimulate PGE2 production of human dental pulp cells (P < 0.05). IL‐1β stimulated the COX‐2 but not COX‐1 mRNA expression. Pulp cells express mainly EP2, EP3 and EP1 receptors as analysed by RT‐PCR. PGE2 (0.25–2 μmol L−1) stimulated the Ca2+ mobilization as indicated by increase in fura‐2 fluorescence.
Conclusions Interleukin‐1β and TNF‐α may stimulate PGE2 production in dental pulp cells. Activation of prostaglandin EP receptors in dental pulp cells by PGE2 may induce Ca2+ signalling to regulate cellular biological activity during inflammation.</description><identifier>ISSN: 0143-2885</identifier><identifier>EISSN: 1365-2591</identifier><identifier>DOI: 10.1111/j.1365-2591.2006.01156.x</identifier><identifier>PMID: 16948668</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>calcium mobilization ; Calcium Signaling - physiology ; Cells, Cultured ; Cyclooxygenase 2 - biosynthesis ; Cyclooxygenase 2 - genetics ; Cytokines - physiology ; Dental Pulp - cytology ; Dental Pulp - metabolism ; Dentistry ; Dinoprostone - biosynthesis ; Dinoprostone - genetics ; Dinoprostone - physiology ; Enzyme Activation ; Enzyme-Linked Immunosorbent Assay ; Fluorescent Dyes ; Fura-2 - analogs & derivatives ; Gene Expression Regulation ; Humans ; inflammation ; Interleukin-1 - physiology ; prostaglandin ; prostaglandin receptor ; Protein Isoforms ; pulp cells ; Pulpitis - metabolism ; Receptors, Prostaglandin E - physiology ; Reverse Transcriptase Polymerase Chain Reaction ; Statistics, Nonparametric ; Tumor Necrosis Factor-alpha - physiology</subject><ispartof>International endodontic journal, 2006-10, Vol.39 (10), p.819-826</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1365-2591.2006.01156.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1365-2591.2006.01156.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27903,27904,45553,45554</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16948668$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chang, M.-C.</creatorcontrib><creatorcontrib>Chen, Y.-J.</creatorcontrib><creatorcontrib>Tai, T.-F.</creatorcontrib><creatorcontrib>Tai, M.-R.</creatorcontrib><creatorcontrib>Li, M.-Y.</creatorcontrib><creatorcontrib>Tsai, Y.-L.</creatorcontrib><creatorcontrib>Lan, W.-H.</creatorcontrib><creatorcontrib>Wang, Y.-L.</creatorcontrib><creatorcontrib>Jeng, J.-H.</creatorcontrib><title>Cytokine-induced prostaglandin E2 production and cyclooxygenase-2 expression in dental pulp cells: downstream calcium signalling via activation of prostaglandin EP receptor</title><title>International endodontic journal</title><addtitle>Int Endod J</addtitle><description>Aim To determine whether (i) proinflammatory cytokines stimulate prostaglandin E2 (PGE2) production and cyclooxygenase (COX) gene expression in dental pulp cells, and (ii) pulp cells that express different prostaglandin E2 receptor (EP) isoforms and their activation by PGE2 leads to downstream Ca2+ signalling.
Methodology Cultured human dental pulp cells were exposed to interleukin (IL)‐1β and tumour necrotic factor‐alpha (TNF‐α). The expression of COX‐1 and COX‐2 was measured with reverse transcriptase‐polymerase chain reaction (RT‐PCR). The production of PGE2 was measured using an enzyme‐linked immunosorbent assay. Expression of prostaglandin EP receptor isoforms was studied by RT‐PCR, whereas fura‐2 fluorescence was used to measure calcium mobilization. The Kruskal–Wallis test and Wilcoxon sum rank test with Bonferroni correction were used for statistical analysis.
Results Interleukin‐1β and TNF‐α stimulate PGE2 production of human dental pulp cells (P < 0.05). IL‐1β stimulated the COX‐2 but not COX‐1 mRNA expression. Pulp cells express mainly EP2, EP3 and EP1 receptors as analysed by RT‐PCR. PGE2 (0.25–2 μmol L−1) stimulated the Ca2+ mobilization as indicated by increase in fura‐2 fluorescence.
Conclusions Interleukin‐1β and TNF‐α may stimulate PGE2 production in dental pulp cells. Activation of prostaglandin EP receptors in dental pulp cells by PGE2 may induce Ca2+ signalling to regulate cellular biological activity during inflammation.</description><subject>calcium mobilization</subject><subject>Calcium Signaling - physiology</subject><subject>Cells, Cultured</subject><subject>Cyclooxygenase 2 - biosynthesis</subject><subject>Cyclooxygenase 2 - genetics</subject><subject>Cytokines - physiology</subject><subject>Dental Pulp - cytology</subject><subject>Dental Pulp - metabolism</subject><subject>Dentistry</subject><subject>Dinoprostone - biosynthesis</subject><subject>Dinoprostone - genetics</subject><subject>Dinoprostone - physiology</subject><subject>Enzyme Activation</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Fluorescent Dyes</subject><subject>Fura-2 - analogs & derivatives</subject><subject>Gene Expression Regulation</subject><subject>Humans</subject><subject>inflammation</subject><subject>Interleukin-1 - physiology</subject><subject>prostaglandin</subject><subject>prostaglandin receptor</subject><subject>Protein Isoforms</subject><subject>pulp cells</subject><subject>Pulpitis - metabolism</subject><subject>Receptors, Prostaglandin E - physiology</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>Statistics, Nonparametric</subject><subject>Tumor Necrosis Factor-alpha - physiology</subject><issn>0143-2885</issn><issn>1365-2591</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkc1u1DAUhS0EokPhFZBX7BL8EzsJEgsYTX-GqnQBYml57JuRp44T4qRN3omHJOm0RcIbW_ecc33tDyFMSUrn9fGQUi5FwkRJU0aITAmlQqbjC7R6Fl6iFaEZT1hRiBP0JsYDIUQQTl-jEyrLrJCyWKE_66lvbl2AxAU7GLC47ZrY673XwbqAN2wpzErvmoDnGjaT8U0zTnsIOkLCMIxtBzEu-hywEHrtcTv4FhvwPn7CtrkPse9A19hob9xQ4-j2QXvvwh7fOY313P5OP1zRVP9PcIM7MND2TfcWvaq0j_DucT9FP882P9YXydX388v1l6vEMSploiGDHatyrrWpKqN5zkVWGqEzJmyZW82MqTJe0HJnSbHLLFCSZ8byzBgpKeOn6MOx7zzJ7wFir2oXl8foAM0QlSwKRvNCzMb3j8ZhV4NVbedq3U3q6X9nw-ej4d55mP7pRC0c1UEtuNSCSy0c1QNHNarLzXY5zfnkmHexh_E5r7tbJXOeC_Xr-lyJb9fbrzfbtTrjfwHR7aV7</recordid><startdate>200610</startdate><enddate>200610</enddate><creator>Chang, M.-C.</creator><creator>Chen, Y.-J.</creator><creator>Tai, T.-F.</creator><creator>Tai, M.-R.</creator><creator>Li, M.-Y.</creator><creator>Tsai, Y.-L.</creator><creator>Lan, W.-H.</creator><creator>Wang, Y.-L.</creator><creator>Jeng, J.-H.</creator><general>Blackwell Publishing Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>200610</creationdate><title>Cytokine-induced prostaglandin E2 production and cyclooxygenase-2 expression in dental pulp cells: downstream calcium signalling via activation of prostaglandin EP receptor</title><author>Chang, M.-C. ; Chen, Y.-J. ; Tai, T.-F. ; Tai, M.-R. ; Li, M.-Y. ; Tsai, Y.-L. ; Lan, W.-H. ; Wang, Y.-L. ; Jeng, J.-H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-i2166-ae4eb2f73aacffca373549c5a425d97da2ccf43819bd08b4de1074cd34cc66123</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>calcium mobilization</topic><topic>Calcium Signaling - physiology</topic><topic>Cells, Cultured</topic><topic>Cyclooxygenase 2 - biosynthesis</topic><topic>Cyclooxygenase 2 - genetics</topic><topic>Cytokines - physiology</topic><topic>Dental Pulp - cytology</topic><topic>Dental Pulp - metabolism</topic><topic>Dentistry</topic><topic>Dinoprostone - biosynthesis</topic><topic>Dinoprostone - genetics</topic><topic>Dinoprostone - physiology</topic><topic>Enzyme Activation</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Fluorescent Dyes</topic><topic>Fura-2 - analogs & derivatives</topic><topic>Gene Expression Regulation</topic><topic>Humans</topic><topic>inflammation</topic><topic>Interleukin-1 - physiology</topic><topic>prostaglandin</topic><topic>prostaglandin receptor</topic><topic>Protein Isoforms</topic><topic>pulp cells</topic><topic>Pulpitis - metabolism</topic><topic>Receptors, Prostaglandin E - physiology</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>Statistics, Nonparametric</topic><topic>Tumor Necrosis Factor-alpha - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chang, M.-C.</creatorcontrib><creatorcontrib>Chen, Y.-J.</creatorcontrib><creatorcontrib>Tai, T.-F.</creatorcontrib><creatorcontrib>Tai, M.-R.</creatorcontrib><creatorcontrib>Li, M.-Y.</creatorcontrib><creatorcontrib>Tsai, Y.-L.</creatorcontrib><creatorcontrib>Lan, W.-H.</creatorcontrib><creatorcontrib>Wang, Y.-L.</creatorcontrib><creatorcontrib>Jeng, J.-H.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>International endodontic journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chang, M.-C.</au><au>Chen, Y.-J.</au><au>Tai, T.-F.</au><au>Tai, M.-R.</au><au>Li, M.-Y.</au><au>Tsai, Y.-L.</au><au>Lan, W.-H.</au><au>Wang, Y.-L.</au><au>Jeng, J.-H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cytokine-induced prostaglandin E2 production and cyclooxygenase-2 expression in dental pulp cells: downstream calcium signalling via activation of prostaglandin EP receptor</atitle><jtitle>International endodontic journal</jtitle><addtitle>Int Endod J</addtitle><date>2006-10</date><risdate>2006</risdate><volume>39</volume><issue>10</issue><spage>819</spage><epage>826</epage><pages>819-826</pages><issn>0143-2885</issn><eissn>1365-2591</eissn><abstract>Aim To determine whether (i) proinflammatory cytokines stimulate prostaglandin E2 (PGE2) production and cyclooxygenase (COX) gene expression in dental pulp cells, and (ii) pulp cells that express different prostaglandin E2 receptor (EP) isoforms and their activation by PGE2 leads to downstream Ca2+ signalling.
Methodology Cultured human dental pulp cells were exposed to interleukin (IL)‐1β and tumour necrotic factor‐alpha (TNF‐α). The expression of COX‐1 and COX‐2 was measured with reverse transcriptase‐polymerase chain reaction (RT‐PCR). The production of PGE2 was measured using an enzyme‐linked immunosorbent assay. Expression of prostaglandin EP receptor isoforms was studied by RT‐PCR, whereas fura‐2 fluorescence was used to measure calcium mobilization. The Kruskal–Wallis test and Wilcoxon sum rank test with Bonferroni correction were used for statistical analysis.
Results Interleukin‐1β and TNF‐α stimulate PGE2 production of human dental pulp cells (P < 0.05). IL‐1β stimulated the COX‐2 but not COX‐1 mRNA expression. Pulp cells express mainly EP2, EP3 and EP1 receptors as analysed by RT‐PCR. PGE2 (0.25–2 μmol L−1) stimulated the Ca2+ mobilization as indicated by increase in fura‐2 fluorescence.
Conclusions Interleukin‐1β and TNF‐α may stimulate PGE2 production in dental pulp cells. Activation of prostaglandin EP receptors in dental pulp cells by PGE2 may induce Ca2+ signalling to regulate cellular biological activity during inflammation.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>16948668</pmid><doi>10.1111/j.1365-2591.2006.01156.x</doi><tpages>8</tpages></addata></record> |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | calcium mobilization Calcium Signaling - physiology Cells, Cultured Cyclooxygenase 2 - biosynthesis Cyclooxygenase 2 - genetics Cytokines - physiology Dental Pulp - cytology Dental Pulp - metabolism Dentistry Dinoprostone - biosynthesis Dinoprostone - genetics Dinoprostone - physiology Enzyme Activation Enzyme-Linked Immunosorbent Assay Fluorescent Dyes Fura-2 - analogs & derivatives Gene Expression Regulation Humans inflammation Interleukin-1 - physiology prostaglandin prostaglandin receptor Protein Isoforms pulp cells Pulpitis - metabolism Receptors, Prostaglandin E - physiology Reverse Transcriptase Polymerase Chain Reaction Statistics, Nonparametric Tumor Necrosis Factor-alpha - physiology |
| title | Cytokine-induced prostaglandin E2 production and cyclooxygenase-2 expression in dental pulp cells: downstream calcium signalling via activation of prostaglandin EP receptor |
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