Investigation of the endosperm-specific sucrose synthase promoter from rice using transient expression of reporter genes in guar seed tissue
We report the investigation of an endosperm-specific promoter from the rsus3 gene from rice (Oryza sativa). The promoter was characterized by deletion analysis and transient expression in guar (Cyamopsis tetragonoloba) seed-tissue. Transient expression was monitored by histochemical GUS assay, and q...
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| Veröffentlicht in: | Plant cell reports 2006-10, Vol.25 (10), p.1035-1042 |
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| creator | Rasmussen, T.B Donaldson, I.A |
| description | We report the investigation of an endosperm-specific promoter from the rsus3 gene from rice (Oryza sativa). The promoter was characterized by deletion analysis and transient expression in guar (Cyamopsis tetragonoloba) seed-tissue. Transient expression was monitored by histochemical GUS assay, and quantitative dual reporter assays comprising firefly luciferase as a test reporter, and Renilla luciferase and GUS as reference reporters. These revealed high expression levels of the reporter genes directed by the rsus3 promoter in guar endosperm. Specificity for this tissue in seeds was apparent from a virtual absence of reporter activity in guar cotyledons. Removal of a putative intron region only slightly raised the expression level, whereas duplication of the minimal promoter region, in a tandem-repeat rsus3 promoter construct, retained endosperm specificity in guar, and displayed three times the reporter activity observed with the single copy construct. |
| doi_str_mv | 10.1007/s00299-006-0158-x |
| format | Article |
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The promoter was characterized by deletion analysis and transient expression in guar (Cyamopsis tetragonoloba) seed-tissue. Transient expression was monitored by histochemical GUS assay, and quantitative dual reporter assays comprising firefly luciferase as a test reporter, and Renilla luciferase and GUS as reference reporters. These revealed high expression levels of the reporter genes directed by the rsus3 promoter in guar endosperm. Specificity for this tissue in seeds was apparent from a virtual absence of reporter activity in guar cotyledons. Removal of a putative intron region only slightly raised the expression level, whereas duplication of the minimal promoter region, in a tandem-repeat rsus3 promoter construct, retained endosperm specificity in guar, and displayed three times the reporter activity observed with the single copy construct.</description><identifier>ISSN: 0721-7714</identifier><identifier>EISSN: 1432-203X</identifier><identifier>DOI: 10.1007/s00299-006-0158-x</identifier><identifier>PMID: 16670901</identifier><identifier>CODEN: PCRPD8</identifier><language>eng</language><publisher>Berlin: Springer</publisher><subject>Base Sequence ; beta-glucuronidase ; Biological and medical sciences ; Biotechnology ; Cyamopsis - genetics ; Cyamopsis tetragonoloba ; endosperm ; Fundamental and applied biological sciences. Psychology ; Gene Expression ; gene expression regulation ; Gene Expression Regulation, Plant ; Genes ; Genes, Plant - genetics ; Genes, Reporter - genetics ; Genetic engineering ; Genetic technics ; Glucosyltransferases - genetics ; Glucuronidase - metabolism ; Introns - genetics ; luciferase ; Methods. Procedures. Technologies ; molecular cloning ; Molecular Sequence Data ; nucleotide sequences ; Oryza - genetics ; Oryza sativa ; Plants, Genetically Modified ; promoter regions ; Promoter Regions, Genetic - genetics ; Proteins ; reporter genes ; rice ; seeds ; Seeds - cytology ; Seeds - enzymology ; sucrose synthase ; Transgenic animals and transgenic plants ; Transgenic plants</subject><ispartof>Plant cell reports, 2006-10, Vol.25 (10), p.1035-1042</ispartof><rights>2007 INIST-CNRS</rights><rights>Springer-Verlag 2006</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c477t-edef08b72ebf6725ed569874bd6e108e1136a238aa1ccca4d2add8b5409bbf293</citedby><cites>FETCH-LOGICAL-c477t-edef08b72ebf6725ed569874bd6e108e1136a238aa1ccca4d2add8b5409bbf293</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18172864$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16670901$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rasmussen, T.B</creatorcontrib><creatorcontrib>Donaldson, I.A</creatorcontrib><title>Investigation of the endosperm-specific sucrose synthase promoter from rice using transient expression of reporter genes in guar seed tissue</title><title>Plant cell reports</title><addtitle>Plant Cell Rep</addtitle><description>We report the investigation of an endosperm-specific promoter from the rsus3 gene from rice (Oryza sativa). The promoter was characterized by deletion analysis and transient expression in guar (Cyamopsis tetragonoloba) seed-tissue. Transient expression was monitored by histochemical GUS assay, and quantitative dual reporter assays comprising firefly luciferase as a test reporter, and Renilla luciferase and GUS as reference reporters. These revealed high expression levels of the reporter genes directed by the rsus3 promoter in guar endosperm. Specificity for this tissue in seeds was apparent from a virtual absence of reporter activity in guar cotyledons. Removal of a putative intron region only slightly raised the expression level, whereas duplication of the minimal promoter region, in a tandem-repeat rsus3 promoter construct, retained endosperm specificity in guar, and displayed three times the reporter activity observed with the single copy construct.</description><subject>Base Sequence</subject><subject>beta-glucuronidase</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Cyamopsis - genetics</subject><subject>Cyamopsis tetragonoloba</subject><subject>endosperm</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression</subject><subject>gene expression regulation</subject><subject>Gene Expression Regulation, Plant</subject><subject>Genes</subject><subject>Genes, Plant - genetics</subject><subject>Genes, Reporter - genetics</subject><subject>Genetic engineering</subject><subject>Genetic technics</subject><subject>Glucosyltransferases - genetics</subject><subject>Glucuronidase - metabolism</subject><subject>Introns - genetics</subject><subject>luciferase</subject><subject>Methods. Procedures. Technologies</subject><subject>molecular cloning</subject><subject>Molecular Sequence Data</subject><subject>nucleotide sequences</subject><subject>Oryza - genetics</subject><subject>Oryza sativa</subject><subject>Plants, Genetically Modified</subject><subject>promoter regions</subject><subject>Promoter Regions, Genetic - genetics</subject><subject>Proteins</subject><subject>reporter genes</subject><subject>rice</subject><subject>seeds</subject><subject>Seeds - cytology</subject><subject>Seeds - enzymology</subject><subject>sucrose synthase</subject><subject>Transgenic animals and transgenic plants</subject><subject>Transgenic plants</subject><issn>0721-7714</issn><issn>1432-203X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqFkUFv1DAQhSMEotvCD-ACFhLcAjNOYjtHVEGpVIkDVOJmOc4kdbXrLJ4Ebf8DPxqvNlIlLlzskfy9Z715RfEK4QMC6I8MINu2BFAlYGPKw5Nig3UlSwnVz6fFBrTEUmusz4pz5nuA_KjV8-IMldLQAm6KP9fxN_EcRjeHKYppEPMdCYr9xHtKuzKfPgzBC158mpgEP8T5zuVhn6bdNFMSQx5ECp7EwiGOYk4ucqA4CzrsEzGvxon2UzoKRorEIkQxLi4JJurFHJgXelE8G9yW6eV6XxS3Xz7_uPxa3ny7ur78dFP6Wuu5pJ4GMJ2W1A1Ky4b6RrVG112vCMEQYqWcrIxz6L13dS9d35uuqaHtukG21UXx_uSbM_xacny7C-xpu3WRpoWtMgZblW3-B2JbNQ2aKoNv_wHvpyXFHMIa0A2CbJoM4Qk6bpITDXafws6lB4tgj4XaU6E2F2qPhdpD1rxejZduR_2jYm0wA-9WwLF32yEv3wd-5AxqaVSduTcnbnCTdWPKzO13mQ0A89coVfUXwRC1vQ</recordid><startdate>20061001</startdate><enddate>20061001</enddate><creator>Rasmussen, T.B</creator><creator>Donaldson, I.A</creator><general>Springer</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T5</scope><scope>7T7</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7QO</scope><scope>7X8</scope></search><sort><creationdate>20061001</creationdate><title>Investigation of the endosperm-specific sucrose synthase promoter from rice using transient expression of reporter genes in guar seed tissue</title><author>Rasmussen, T.B ; Donaldson, I.A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c477t-edef08b72ebf6725ed569874bd6e108e1136a238aa1ccca4d2add8b5409bbf293</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Base Sequence</topic><topic>beta-glucuronidase</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Cyamopsis - genetics</topic><topic>Cyamopsis tetragonoloba</topic><topic>endosperm</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression</topic><topic>gene expression regulation</topic><topic>Gene Expression Regulation, Plant</topic><topic>Genes</topic><topic>Genes, Plant - genetics</topic><topic>Genes, Reporter - genetics</topic><topic>Genetic engineering</topic><topic>Genetic technics</topic><topic>Glucosyltransferases - genetics</topic><topic>Glucuronidase - metabolism</topic><topic>Introns - genetics</topic><topic>luciferase</topic><topic>Methods. Procedures. Technologies</topic><topic>molecular cloning</topic><topic>Molecular Sequence Data</topic><topic>nucleotide sequences</topic><topic>Oryza - genetics</topic><topic>Oryza sativa</topic><topic>Plants, Genetically Modified</topic><topic>promoter regions</topic><topic>Promoter Regions, Genetic - genetics</topic><topic>Proteins</topic><topic>reporter genes</topic><topic>rice</topic><topic>seeds</topic><topic>Seeds - cytology</topic><topic>Seeds - enzymology</topic><topic>sucrose synthase</topic><topic>Transgenic animals and transgenic plants</topic><topic>Transgenic plants</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rasmussen, T.B</creatorcontrib><creatorcontrib>Donaldson, I.A</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Plant cell reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rasmussen, T.B</au><au>Donaldson, I.A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Investigation of the endosperm-specific sucrose synthase promoter from rice using transient expression of reporter genes in guar seed tissue</atitle><jtitle>Plant cell reports</jtitle><addtitle>Plant Cell Rep</addtitle><date>2006-10-01</date><risdate>2006</risdate><volume>25</volume><issue>10</issue><spage>1035</spage><epage>1042</epage><pages>1035-1042</pages><issn>0721-7714</issn><eissn>1432-203X</eissn><coden>PCRPD8</coden><abstract>We report the investigation of an endosperm-specific promoter from the rsus3 gene from rice (Oryza sativa). The promoter was characterized by deletion analysis and transient expression in guar (Cyamopsis tetragonoloba) seed-tissue. Transient expression was monitored by histochemical GUS assay, and quantitative dual reporter assays comprising firefly luciferase as a test reporter, and Renilla luciferase and GUS as reference reporters. These revealed high expression levels of the reporter genes directed by the rsus3 promoter in guar endosperm. Specificity for this tissue in seeds was apparent from a virtual absence of reporter activity in guar cotyledons. Removal of a putative intron region only slightly raised the expression level, whereas duplication of the minimal promoter region, in a tandem-repeat rsus3 promoter construct, retained endosperm specificity in guar, and displayed three times the reporter activity observed with the single copy construct.</abstract><cop>Berlin</cop><pub>Springer</pub><pmid>16670901</pmid><doi>10.1007/s00299-006-0158-x</doi><tpages>8</tpages></addata></record> |
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| subjects | Base Sequence beta-glucuronidase Biological and medical sciences Biotechnology Cyamopsis - genetics Cyamopsis tetragonoloba endosperm Fundamental and applied biological sciences. Psychology Gene Expression gene expression regulation Gene Expression Regulation, Plant Genes Genes, Plant - genetics Genes, Reporter - genetics Genetic engineering Genetic technics Glucosyltransferases - genetics Glucuronidase - metabolism Introns - genetics luciferase Methods. Procedures. Technologies molecular cloning Molecular Sequence Data nucleotide sequences Oryza - genetics Oryza sativa Plants, Genetically Modified promoter regions Promoter Regions, Genetic - genetics Proteins reporter genes rice seeds Seeds - cytology Seeds - enzymology sucrose synthase Transgenic animals and transgenic plants Transgenic plants |
| title | Investigation of the endosperm-specific sucrose synthase promoter from rice using transient expression of reporter genes in guar seed tissue |
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