Inhibitory cis-element-mediated decay of human papillomavirus type 16 L1-transcript in undifferentiated cells
Production of human papillomavirus type 16 major capsid protein L1 in undifferentiated cells is negatively regulated by several yet unidentified cis-acting inhibitory RNA elements, among which a major element is located within the first 514 nucleotides of the L1-mRNA. By Northern blotting we examine...
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| Veröffentlicht in: | Molecular and cellular biochemistry 2006-08, Vol.288 (1-2), p.47-57 |
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| creator | Mori, Seiichiro Ozaki, Saori Yasugi, Toshiharu Yoshikawa, Hiroyuki Taketani, Yuji Kanda, Tadahito |
| description | Production of human papillomavirus type 16 major capsid protein L1 in undifferentiated cells is negatively regulated by several yet unidentified cis-acting inhibitory RNA elements, among which a major element is located within the first 514 nucleotides of the L1-mRNA. By Northern blotting we examined effect of the major element on the steady-state level of mRNA transiently transcribed in 293T cells from the firefly luciferase (Fluc) gene combined with the L1 DNA fragment encoding the major element. As reported previously, the element down-regulated steady-state level of the mRNA. The most efficient down-regulation was achieved by insertion of the element near the 5' end of mRNA, resulting in an undetectable level of the mRNA. The longer the distance from the 5' end of the mRNA to the element, the weaker the down-regulation. The half-life of the mRNA having the element was similar to that of normal Fluc-mRNA. When the element near the 5' end was removed by splicing, the steady-state level of the resultant mRNA was raised to a readily detectable level. The steady-state level of RNA synthesized by RNA polymerase-I was not influenced by the presence of the element. Taken together, it is suggested that DNA region encoding the major inhibitory element does not disturb transcription and that the pre-mRNA is degraded by an RNA element-mediated mechanism after the splicing step in the course of mRNA maturation. |
| doi_str_mv | 10.1007/s11010-006-9117-7 |
| format | Article |
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By Northern blotting we examined effect of the major element on the steady-state level of mRNA transiently transcribed in 293T cells from the firefly luciferase (Fluc) gene combined with the L1 DNA fragment encoding the major element. As reported previously, the element down-regulated steady-state level of the mRNA. The most efficient down-regulation was achieved by insertion of the element near the 5' end of mRNA, resulting in an undetectable level of the mRNA. The longer the distance from the 5' end of the mRNA to the element, the weaker the down-regulation. The half-life of the mRNA having the element was similar to that of normal Fluc-mRNA. When the element near the 5' end was removed by splicing, the steady-state level of the resultant mRNA was raised to a readily detectable level. The steady-state level of RNA synthesized by RNA polymerase-I was not influenced by the presence of the element. Taken together, it is suggested that DNA region encoding the major inhibitory element does not disturb transcription and that the pre-mRNA is degraded by an RNA element-mediated mechanism after the splicing step in the course of mRNA maturation.</description><identifier>ISSN: 0300-8177</identifier><identifier>EISSN: 1573-4919</identifier><identifier>DOI: 10.1007/s11010-006-9117-7</identifier><identifier>PMID: 16583140</identifier><language>eng</language><publisher>Netherlands: Springer Nature B.V</publisher><subject>Capsid protein ; Capsid Proteins - genetics ; Capsid Proteins - metabolism ; Cells, Cultured ; Codon - metabolism ; Deoxyribonucleic acid ; DNA ; DNA-directed RNA polymerase ; Down-Regulation ; Gene Expression Regulation, Viral ; Human papillomavirus ; Human papillomavirus 16 ; Humans ; Mutation ; Northern blotting ; Nucleotides ; Oncogene Proteins, Viral - genetics ; Oncogene Proteins, Viral - metabolism ; Regulatory Sequences, Ribonucleic Acid ; Ribonucleic acid ; RNA ; RNA polymerase ; RNA Polymerase I - metabolism ; RNA Splicing ; RNA Stability ; RNA, Messenger - chemistry ; RNA, Messenger - metabolism ; Splicing ; Steady state ; Transcription ; Transfection</subject><ispartof>Molecular and cellular biochemistry, 2006-08, Vol.288 (1-2), p.47-57</ispartof><rights>Springer Science+Business Media, Inc. 2006.</rights><rights>Springer Science+Business Media, LLC 2006</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c484t-19f2507d631176761bd3e3c59354b6a978771f618837f57ae4b49cc369b15a7b3</citedby><cites>FETCH-LOGICAL-c484t-19f2507d631176761bd3e3c59354b6a978771f618837f57ae4b49cc369b15a7b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27922,27923</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16583140$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mori, Seiichiro</creatorcontrib><creatorcontrib>Ozaki, Saori</creatorcontrib><creatorcontrib>Yasugi, Toshiharu</creatorcontrib><creatorcontrib>Yoshikawa, Hiroyuki</creatorcontrib><creatorcontrib>Taketani, Yuji</creatorcontrib><creatorcontrib>Kanda, Tadahito</creatorcontrib><title>Inhibitory cis-element-mediated decay of human papillomavirus type 16 L1-transcript in undifferentiated cells</title><title>Molecular and cellular biochemistry</title><addtitle>Mol Cell Biochem</addtitle><description>Production of human papillomavirus type 16 major capsid protein L1 in undifferentiated cells is negatively regulated by several yet unidentified cis-acting inhibitory RNA elements, among which a major element is located within the first 514 nucleotides of the L1-mRNA. By Northern blotting we examined effect of the major element on the steady-state level of mRNA transiently transcribed in 293T cells from the firefly luciferase (Fluc) gene combined with the L1 DNA fragment encoding the major element. As reported previously, the element down-regulated steady-state level of the mRNA. The most efficient down-regulation was achieved by insertion of the element near the 5' end of mRNA, resulting in an undetectable level of the mRNA. The longer the distance from the 5' end of the mRNA to the element, the weaker the down-regulation. The half-life of the mRNA having the element was similar to that of normal Fluc-mRNA. When the element near the 5' end was removed by splicing, the steady-state level of the resultant mRNA was raised to a readily detectable level. The steady-state level of RNA synthesized by RNA polymerase-I was not influenced by the presence of the element. Taken together, it is suggested that DNA region encoding the major inhibitory element does not disturb transcription and that the pre-mRNA is degraded by an RNA element-mediated mechanism after the splicing step in the course of mRNA maturation.</description><subject>Capsid protein</subject><subject>Capsid Proteins - genetics</subject><subject>Capsid Proteins - metabolism</subject><subject>Cells, Cultured</subject><subject>Codon - metabolism</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA-directed RNA polymerase</subject><subject>Down-Regulation</subject><subject>Gene Expression Regulation, Viral</subject><subject>Human papillomavirus</subject><subject>Human papillomavirus 16</subject><subject>Humans</subject><subject>Mutation</subject><subject>Northern blotting</subject><subject>Nucleotides</subject><subject>Oncogene Proteins, Viral - genetics</subject><subject>Oncogene Proteins, Viral - metabolism</subject><subject>Regulatory Sequences, Ribonucleic Acid</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>RNA polymerase</subject><subject>RNA Polymerase I - metabolism</subject><subject>RNA Splicing</subject><subject>RNA Stability</subject><subject>RNA, Messenger - chemistry</subject><subject>RNA, Messenger - metabolism</subject><subject>Splicing</subject><subject>Steady state</subject><subject>Transcription</subject><subject>Transfection</subject><issn>0300-8177</issn><issn>1573-4919</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFkk2LFDEQhoO4uOPqD_AiQUG8RFOdj0qOsuzqwoAXPYd0Os1m6S-TbmH-_WacAUFQT3V56qkq6iXkFfAPwDl-LAAcOONcMwuADJ-QHSgUTFqwT8mOC86ZAcRL8ryUB15hDvCMXIJWRoDkOzLeTfepTeucDzSkwuIQxzitbIxd8mvsaBeDP9C5p_fb6Ce6-CUNwzz6nylvha6HJVLQdA9szX4qIadlpWmi29Slvo-5uk6eEIehvCAXvR9KfHmuV-T77c236y9s__Xz3fWnPQvSyJWB7RvFsdOiXqVRQ9uJKIKyQslWe4sGEXoNxgjsFfooW2lDENq2oDy24oq8O3mXPP_YYlndmMpxAz_FeStOGwOq4ea_IFippbWigu__DTYKjTL2l_PNH-jDvOWp3utQ6YY3Em2F3v4NarSthAJ1nAonKuS5lBx7t-Q0-nxwwN0xAu4UAVcj4I4RcFh7Xp_NW1u_-Lvj_HPxCHRCqfU</recordid><startdate>20060801</startdate><enddate>20060801</enddate><creator>Mori, Seiichiro</creator><creator>Ozaki, Saori</creator><creator>Yasugi, Toshiharu</creator><creator>Yoshikawa, Hiroyuki</creator><creator>Taketani, Yuji</creator><creator>Kanda, Tadahito</creator><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QP</scope><scope>7T5</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20060801</creationdate><title>Inhibitory cis-element-mediated decay of human papillomavirus type 16 L1-transcript in undifferentiated cells</title><author>Mori, Seiichiro ; Ozaki, Saori ; Yasugi, Toshiharu ; Yoshikawa, Hiroyuki ; Taketani, Yuji ; Kanda, Tadahito</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c484t-19f2507d631176761bd3e3c59354b6a978771f618837f57ae4b49cc369b15a7b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Capsid protein</topic><topic>Capsid Proteins - 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Academic</collection><jtitle>Molecular and cellular biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mori, Seiichiro</au><au>Ozaki, Saori</au><au>Yasugi, Toshiharu</au><au>Yoshikawa, Hiroyuki</au><au>Taketani, Yuji</au><au>Kanda, Tadahito</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Inhibitory cis-element-mediated decay of human papillomavirus type 16 L1-transcript in undifferentiated cells</atitle><jtitle>Molecular and cellular biochemistry</jtitle><addtitle>Mol Cell Biochem</addtitle><date>2006-08-01</date><risdate>2006</risdate><volume>288</volume><issue>1-2</issue><spage>47</spage><epage>57</epage><pages>47-57</pages><issn>0300-8177</issn><eissn>1573-4919</eissn><abstract>Production of human papillomavirus type 16 major capsid protein L1 in undifferentiated cells is negatively regulated by several yet unidentified cis-acting inhibitory RNA elements, among which a major element is located within the first 514 nucleotides of the L1-mRNA. By Northern blotting we examined effect of the major element on the steady-state level of mRNA transiently transcribed in 293T cells from the firefly luciferase (Fluc) gene combined with the L1 DNA fragment encoding the major element. As reported previously, the element down-regulated steady-state level of the mRNA. The most efficient down-regulation was achieved by insertion of the element near the 5' end of mRNA, resulting in an undetectable level of the mRNA. The longer the distance from the 5' end of the mRNA to the element, the weaker the down-regulation. The half-life of the mRNA having the element was similar to that of normal Fluc-mRNA. When the element near the 5' end was removed by splicing, the steady-state level of the resultant mRNA was raised to a readily detectable level. The steady-state level of RNA synthesized by RNA polymerase-I was not influenced by the presence of the element. Taken together, it is suggested that DNA region encoding the major inhibitory element does not disturb transcription and that the pre-mRNA is degraded by an RNA element-mediated mechanism after the splicing step in the course of mRNA maturation.</abstract><cop>Netherlands</cop><pub>Springer Nature B.V</pub><pmid>16583140</pmid><doi>10.1007/s11010-006-9117-7</doi><tpages>11</tpages></addata></record> |
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| subjects | Capsid protein Capsid Proteins - genetics Capsid Proteins - metabolism Cells, Cultured Codon - metabolism Deoxyribonucleic acid DNA DNA-directed RNA polymerase Down-Regulation Gene Expression Regulation, Viral Human papillomavirus Human papillomavirus 16 Humans Mutation Northern blotting Nucleotides Oncogene Proteins, Viral - genetics Oncogene Proteins, Viral - metabolism Regulatory Sequences, Ribonucleic Acid Ribonucleic acid RNA RNA polymerase RNA Polymerase I - metabolism RNA Splicing RNA Stability RNA, Messenger - chemistry RNA, Messenger - metabolism Splicing Steady state Transcription Transfection |
| title | Inhibitory cis-element-mediated decay of human papillomavirus type 16 L1-transcript in undifferentiated cells |
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