Enhancement of de Novo Fatty Acid Biosynthesis in Hepatic Cell Line Huh7 Expressing Hepatitis C Virus Core Protein
Hepatitis C virus (HCV) core protein plays important roles in the pathogeneses of liver steatosis as well as hepatocellular carcinomas due to HCV infection. In this study, we examined de novo fatty acid biosynthesis in hepatic cell line Huh7 cells expressing HCV core protein. The rate of metabolic l...
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| Veröffentlicht in: | Biological & pharmaceutical bulletin 2006, Vol.29(9), pp.1958-1961 |
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| creator | Fukasawa, Masayoshi Tanaka, Yasuhito Sato, Shigeko Ono, Yujin Nitahara-Kasahara, Yuko Suzuki, Tetsuro Miyamura, Tatsuo Hanada, Kentaro Nishijima, Masahiro |
| description | Hepatitis C virus (HCV) core protein plays important roles in the pathogeneses of liver steatosis as well as hepatocellular carcinomas due to HCV infection. In this study, we examined de novo fatty acid biosynthesis in hepatic cell line Huh7 cells expressing HCV core protein. The rate of metabolic labeling of cellular fatty acids with [3H]acetate in core-expressing (Uc39-6) cells was ca. 1.5-fold higher than that in non-expressing (Uc321) cells. The enzyme activities responsible for fatty acid biosynthesis were assayed in vitro. Cytosolic acetyl-CoA carboxylase activity in Uc39-6 cells was ca. 1.6-fold higher than that in Uc321 cells. On the other hand, cytosolic fatty acid synthase activity in Uc39-6 cells was only slightly higher than that in Uc321 cells. Immunoblot analysis of acetyl-CoA carboxylase 1 (ACC1), which is a rate-limiting enzyme for fatty acid biosynthesis, revealed a higher expression level of the protein in Uc39-6 cells than in Uc321 cells. The ACC1 mRNA content in Uc39-6 cells was 1.4-fold higher than that in Uc321 cells. These results strongly suggest that enhancement of fatty acid biosynthesis in core-expressing cells is caused by increased expression of fatty acid biosynthetic enzymes, especially ACC1. Up-regulation of de novo fatty acid biosynthesis by HCV core protein may affect cellular lipid metabolism, resulting in neutral lipid accumulation in HCV-infected cells. |
| doi_str_mv | 10.1248/bpb.29.1958 |
| format | Article |
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In this study, we examined de novo fatty acid biosynthesis in hepatic cell line Huh7 cells expressing HCV core protein. The rate of metabolic labeling of cellular fatty acids with [3H]acetate in core-expressing (Uc39-6) cells was ca. 1.5-fold higher than that in non-expressing (Uc321) cells. The enzyme activities responsible for fatty acid biosynthesis were assayed in vitro. Cytosolic acetyl-CoA carboxylase activity in Uc39-6 cells was ca. 1.6-fold higher than that in Uc321 cells. On the other hand, cytosolic fatty acid synthase activity in Uc39-6 cells was only slightly higher than that in Uc321 cells. Immunoblot analysis of acetyl-CoA carboxylase 1 (ACC1), which is a rate-limiting enzyme for fatty acid biosynthesis, revealed a higher expression level of the protein in Uc39-6 cells than in Uc321 cells. The ACC1 mRNA content in Uc39-6 cells was 1.4-fold higher than that in Uc321 cells. These results strongly suggest that enhancement of fatty acid biosynthesis in core-expressing cells is caused by increased expression of fatty acid biosynthetic enzymes, especially ACC1. Up-regulation of de novo fatty acid biosynthesis by HCV core protein may affect cellular lipid metabolism, resulting in neutral lipid accumulation in HCV-infected cells.</description><identifier>ISSN: 0918-6158</identifier><identifier>EISSN: 1347-5215</identifier><identifier>DOI: 10.1248/bpb.29.1958</identifier><identifier>PMID: 16946517</identifier><language>eng</language><publisher>Japan: The Pharmaceutical Society of Japan</publisher><subject>acetyl-CoA carboxylase ; Cell Line ; fatty acid biosynthesis ; fatty acid synthase ; Fatty Acid Synthases - genetics ; Fatty Acid Synthases - metabolism ; Fatty Acids - biosynthesis ; Fatty Liver - etiology ; Fatty Liver - prevention & control ; Hepatitis C ; Hepatitis C - complications ; Hepatitis C virus ; Humans ; Liver - metabolism ; Liver - virology ; Protein Kinases - genetics ; Protein Kinases - metabolism ; RNA, Messenger - analysis ; Viral Core Proteins - physiology</subject><ispartof>Biological and Pharmaceutical Bulletin, 2006, Vol.29(9), pp.1958-1961</ispartof><rights>2006 The Pharmaceutical Society of Japan</rights><rights>Copyright Japan Science and Technology Agency 2006</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c656t-b540ba3bda769da4a332a444ac2e7fe2d991a2be933da7833475e4798c7ed55e3</citedby><cites>FETCH-LOGICAL-c656t-b540ba3bda769da4a332a444ac2e7fe2d991a2be933da7833475e4798c7ed55e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,1876,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16946517$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fukasawa, Masayoshi</creatorcontrib><creatorcontrib>Tanaka, Yasuhito</creatorcontrib><creatorcontrib>Sato, Shigeko</creatorcontrib><creatorcontrib>Ono, Yujin</creatorcontrib><creatorcontrib>Nitahara-Kasahara, Yuko</creatorcontrib><creatorcontrib>Suzuki, Tetsuro</creatorcontrib><creatorcontrib>Miyamura, Tatsuo</creatorcontrib><creatorcontrib>Hanada, Kentaro</creatorcontrib><creatorcontrib>Nishijima, Masahiro</creatorcontrib><title>Enhancement of de Novo Fatty Acid Biosynthesis in Hepatic Cell Line Huh7 Expressing Hepatitis C Virus Core Protein</title><title>Biological & pharmaceutical bulletin</title><addtitle>Biol Pharm Bull</addtitle><description>Hepatitis C virus (HCV) core protein plays important roles in the pathogeneses of liver steatosis as well as hepatocellular carcinomas due to HCV infection. In this study, we examined de novo fatty acid biosynthesis in hepatic cell line Huh7 cells expressing HCV core protein. The rate of metabolic labeling of cellular fatty acids with [3H]acetate in core-expressing (Uc39-6) cells was ca. 1.5-fold higher than that in non-expressing (Uc321) cells. The enzyme activities responsible for fatty acid biosynthesis were assayed in vitro. Cytosolic acetyl-CoA carboxylase activity in Uc39-6 cells was ca. 1.6-fold higher than that in Uc321 cells. On the other hand, cytosolic fatty acid synthase activity in Uc39-6 cells was only slightly higher than that in Uc321 cells. Immunoblot analysis of acetyl-CoA carboxylase 1 (ACC1), which is a rate-limiting enzyme for fatty acid biosynthesis, revealed a higher expression level of the protein in Uc39-6 cells than in Uc321 cells. The ACC1 mRNA content in Uc39-6 cells was 1.4-fold higher than that in Uc321 cells. These results strongly suggest that enhancement of fatty acid biosynthesis in core-expressing cells is caused by increased expression of fatty acid biosynthetic enzymes, especially ACC1. Up-regulation of de novo fatty acid biosynthesis by HCV core protein may affect cellular lipid metabolism, resulting in neutral lipid accumulation in HCV-infected cells.</description><subject>acetyl-CoA carboxylase</subject><subject>Cell Line</subject><subject>fatty acid biosynthesis</subject><subject>fatty acid synthase</subject><subject>Fatty Acid Synthases - genetics</subject><subject>Fatty Acid Synthases - metabolism</subject><subject>Fatty Acids - biosynthesis</subject><subject>Fatty Liver - etiology</subject><subject>Fatty Liver - prevention & control</subject><subject>Hepatitis C</subject><subject>Hepatitis C - complications</subject><subject>Hepatitis C virus</subject><subject>Humans</subject><subject>Liver - metabolism</subject><subject>Liver - virology</subject><subject>Protein Kinases - genetics</subject><subject>Protein Kinases - metabolism</subject><subject>RNA, Messenger - analysis</subject><subject>Viral Core Proteins - physiology</subject><issn>0918-6158</issn><issn>1347-5215</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0U1rGzEQBmARWhI3zan3IijkUtZdfe1KtyTGqQum7aHNVWi141jGljaSNtT_vjJ2U-ilF81BDy8zvAi9I_WUUC4_dUM3pWpKlJBnaEIYbytBiXiFJrUismqIkBfoTUqbuq7bmrJzdEEaxRtB2gmKc7823sIOfMZhhXvAX8NzwPcm5z2-ta7Hdy6kvc9rSC5h5_ECBpOdxTPYbvHSecCLcd3i-a8hQkrOP55ELnyGH1wcywwR8PcYMjj_Fr1emW2Cq9O8RD_v5z9mi2r57fOX2e2yso1octUJXneGdb1pG9UbbhijhnNuLIV2BbRXihjagWKsEMnK3QJ4q6RtoRcC2CW6PuYOMTyNkLLeuWTL0sZDGJNupCScMPFfSBRTjAtV4Id_4CaM0ZcjNOFcMaE4l0V9PCobQ0oRVnqIbmfiXpNaHxrTpTFNlT40VvT7U-bY7aD_a08VFXBzBJuUzSO8ABNLCVv4E6aOzyHz5cuuTdTg2W_kkKfJ</recordid><startdate>20060901</startdate><enddate>20060901</enddate><creator>Fukasawa, Masayoshi</creator><creator>Tanaka, Yasuhito</creator><creator>Sato, Shigeko</creator><creator>Ono, Yujin</creator><creator>Nitahara-Kasahara, Yuko</creator><creator>Suzuki, Tetsuro</creator><creator>Miyamura, Tatsuo</creator><creator>Hanada, Kentaro</creator><creator>Nishijima, Masahiro</creator><general>The Pharmaceutical Society of Japan</general><general>Japan Science and Technology Agency</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20060901</creationdate><title>Enhancement of de Novo Fatty Acid Biosynthesis in Hepatic Cell Line Huh7 Expressing Hepatitis C Virus Core Protein</title><author>Fukasawa, Masayoshi ; Tanaka, Yasuhito ; Sato, Shigeko ; Ono, Yujin ; Nitahara-Kasahara, Yuko ; Suzuki, Tetsuro ; Miyamura, Tatsuo ; Hanada, Kentaro ; Nishijima, Masahiro</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c656t-b540ba3bda769da4a332a444ac2e7fe2d991a2be933da7833475e4798c7ed55e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>acetyl-CoA carboxylase</topic><topic>Cell Line</topic><topic>fatty acid biosynthesis</topic><topic>fatty acid synthase</topic><topic>Fatty Acid Synthases - genetics</topic><topic>Fatty Acid Synthases - metabolism</topic><topic>Fatty Acids - biosynthesis</topic><topic>Fatty Liver - etiology</topic><topic>Fatty Liver - prevention & control</topic><topic>Hepatitis C</topic><topic>Hepatitis C - complications</topic><topic>Hepatitis C virus</topic><topic>Humans</topic><topic>Liver - metabolism</topic><topic>Liver - virology</topic><topic>Protein Kinases - genetics</topic><topic>Protein Kinases - metabolism</topic><topic>RNA, Messenger - analysis</topic><topic>Viral Core Proteins - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fukasawa, Masayoshi</creatorcontrib><creatorcontrib>Tanaka, Yasuhito</creatorcontrib><creatorcontrib>Sato, Shigeko</creatorcontrib><creatorcontrib>Ono, Yujin</creatorcontrib><creatorcontrib>Nitahara-Kasahara, Yuko</creatorcontrib><creatorcontrib>Suzuki, Tetsuro</creatorcontrib><creatorcontrib>Miyamura, Tatsuo</creatorcontrib><creatorcontrib>Hanada, Kentaro</creatorcontrib><creatorcontrib>Nishijima, Masahiro</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biological & pharmaceutical bulletin</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fukasawa, Masayoshi</au><au>Tanaka, Yasuhito</au><au>Sato, Shigeko</au><au>Ono, Yujin</au><au>Nitahara-Kasahara, Yuko</au><au>Suzuki, Tetsuro</au><au>Miyamura, Tatsuo</au><au>Hanada, Kentaro</au><au>Nishijima, Masahiro</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Enhancement of de Novo Fatty Acid Biosynthesis in Hepatic Cell Line Huh7 Expressing Hepatitis C Virus Core Protein</atitle><jtitle>Biological & pharmaceutical bulletin</jtitle><addtitle>Biol Pharm Bull</addtitle><date>2006-09-01</date><risdate>2006</risdate><volume>29</volume><issue>9</issue><spage>1958</spage><epage>1961</epage><pages>1958-1961</pages><issn>0918-6158</issn><eissn>1347-5215</eissn><abstract>Hepatitis C virus (HCV) core protein plays important roles in the pathogeneses of liver steatosis as well as hepatocellular carcinomas due to HCV infection. In this study, we examined de novo fatty acid biosynthesis in hepatic cell line Huh7 cells expressing HCV core protein. The rate of metabolic labeling of cellular fatty acids with [3H]acetate in core-expressing (Uc39-6) cells was ca. 1.5-fold higher than that in non-expressing (Uc321) cells. The enzyme activities responsible for fatty acid biosynthesis were assayed in vitro. Cytosolic acetyl-CoA carboxylase activity in Uc39-6 cells was ca. 1.6-fold higher than that in Uc321 cells. On the other hand, cytosolic fatty acid synthase activity in Uc39-6 cells was only slightly higher than that in Uc321 cells. Immunoblot analysis of acetyl-CoA carboxylase 1 (ACC1), which is a rate-limiting enzyme for fatty acid biosynthesis, revealed a higher expression level of the protein in Uc39-6 cells than in Uc321 cells. The ACC1 mRNA content in Uc39-6 cells was 1.4-fold higher than that in Uc321 cells. These results strongly suggest that enhancement of fatty acid biosynthesis in core-expressing cells is caused by increased expression of fatty acid biosynthetic enzymes, especially ACC1. Up-regulation of de novo fatty acid biosynthesis by HCV core protein may affect cellular lipid metabolism, resulting in neutral lipid accumulation in HCV-infected cells.</abstract><cop>Japan</cop><pub>The Pharmaceutical Society of Japan</pub><pmid>16946517</pmid><doi>10.1248/bpb.29.1958</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Biological and Pharmaceutical Bulletin, 2006, Vol.29(9), pp.1958-1961 |
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| subjects | acetyl-CoA carboxylase Cell Line fatty acid biosynthesis fatty acid synthase Fatty Acid Synthases - genetics Fatty Acid Synthases - metabolism Fatty Acids - biosynthesis Fatty Liver - etiology Fatty Liver - prevention & control Hepatitis C Hepatitis C - complications Hepatitis C virus Humans Liver - metabolism Liver - virology Protein Kinases - genetics Protein Kinases - metabolism RNA, Messenger - analysis Viral Core Proteins - physiology |
| title | Enhancement of de Novo Fatty Acid Biosynthesis in Hepatic Cell Line Huh7 Expressing Hepatitis C Virus Core Protein |
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