Antibiotic susceptibility of mammalian mitochondrial translation
All medically useful antibiotics should have the potential to distinguish between target microbes (bacteria) and host cells. Although many antibiotics that target bacterial protein synthesis show little effect on the translation machinery of the eukaryotic cytoplasm, it is unclear whether these anti...
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| Veröffentlicht in: | FEBS letters 2005-11, Vol.579 (28), p.6423-6427 |
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| creator | Zhang, Li Ging, Ng Ching Komoda, Taeko Hanada, Takao Suzuki, Tsutomu Watanabe, Kimitsuna |
| description | All medically useful antibiotics should have the potential to distinguish between target microbes (bacteria) and host cells. Although many antibiotics that target bacterial protein synthesis show little effect on the translation machinery of the eukaryotic cytoplasm, it is unclear whether these antibiotics target or not the mitochondrial translation machinery. We employed an in vitro translation system from bovine mitochondria, which consists of mitochondrial ribosomes and mitochondrial elongation factors, to estimate the effect of antibiotics on mitichondrial protein synthesis. Tetracycline and thiostrepton showed similar inhibitory effects on both
Escherichia coli and mitochondrial protein synthesis. The mitochondrial system was more resistant to tiamulin, macrolides, virginiamycin, fusidic acid and kirromycin than the
E. coli system. The present results, taken together with atomic structure of the ribosome, may provide useful information for the rational design of new antibiotics having less adverse effects in humans and animals. |
| doi_str_mv | 10.1016/j.febslet.2005.09.103 |
| format | Article |
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Escherichia coli and mitochondrial protein synthesis. The mitochondrial system was more resistant to tiamulin, macrolides, virginiamycin, fusidic acid and kirromycin than the
E. coli system. The present results, taken together with atomic structure of the ribosome, may provide useful information for the rational design of new antibiotics having less adverse effects in humans and animals.</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1016/j.febslet.2005.09.103</identifier><identifier>PMID: 16271719</identifier><language>eng</language><publisher>England: Elsevier B.V</publisher><subject>Animals ; Anti-Bacterial Agents - pharmacology ; Antibiotics ; Cattle ; Drug Resistance, Bacterial ; E. coli ; Escherichia coli ; Escherichia coli - drug effects ; Escherichia coli - genetics ; Mitochondria ; Mitochondria - drug effects ; Mitochondria - metabolism ; Peptide Elongation Factors - drug effects ; Protein Biosynthesis - drug effects ; Protein synthesis ; Ribosome ; Ribosomes - drug effects</subject><ispartof>FEBS letters, 2005-11, Vol.579 (28), p.6423-6427</ispartof><rights>2005</rights><rights>FEBS Letters 579 (2005) 1873-3468 © 2015 Federation of European Biochemical Societies</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c6240-2970aefcbbbaa64d43d3791ebc78fde48aae45fe558c6e81379d7058b0357fc33</citedby><cites>FETCH-LOGICAL-c6240-2970aefcbbbaa64d43d3791ebc78fde48aae45fe558c6e81379d7058b0357fc33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1016%2Fj.febslet.2005.09.103$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0014579305012810$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,776,780,1411,1427,3537,27901,27902,45550,45551,46384,46808,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16271719$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Li</creatorcontrib><creatorcontrib>Ging, Ng Ching</creatorcontrib><creatorcontrib>Komoda, Taeko</creatorcontrib><creatorcontrib>Hanada, Takao</creatorcontrib><creatorcontrib>Suzuki, Tsutomu</creatorcontrib><creatorcontrib>Watanabe, Kimitsuna</creatorcontrib><title>Antibiotic susceptibility of mammalian mitochondrial translation</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>All medically useful antibiotics should have the potential to distinguish between target microbes (bacteria) and host cells. Although many antibiotics that target bacterial protein synthesis show little effect on the translation machinery of the eukaryotic cytoplasm, it is unclear whether these antibiotics target or not the mitochondrial translation machinery. We employed an in vitro translation system from bovine mitochondria, which consists of mitochondrial ribosomes and mitochondrial elongation factors, to estimate the effect of antibiotics on mitichondrial protein synthesis. Tetracycline and thiostrepton showed similar inhibitory effects on both
Escherichia coli and mitochondrial protein synthesis. The mitochondrial system was more resistant to tiamulin, macrolides, virginiamycin, fusidic acid and kirromycin than the
E. coli system. The present results, taken together with atomic structure of the ribosome, may provide useful information for the rational design of new antibiotics having less adverse effects in humans and animals.</description><subject>Animals</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Antibiotics</subject><subject>Cattle</subject><subject>Drug Resistance, Bacterial</subject><subject>E. coli</subject><subject>Escherichia coli</subject><subject>Escherichia coli - drug effects</subject><subject>Escherichia coli - genetics</subject><subject>Mitochondria</subject><subject>Mitochondria - drug effects</subject><subject>Mitochondria - metabolism</subject><subject>Peptide Elongation Factors - drug effects</subject><subject>Protein Biosynthesis - drug effects</subject><subject>Protein synthesis</subject><subject>Ribosome</subject><subject>Ribosomes - drug effects</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkUFv3CAQhVHVqNmm_QmtfOrNm8EYg0_tZrXJRoqUQ9IzwnisssJmC2yi_ffF2pV6TE5oho83jzeEfKOwpECb691ywC46TMsKgC-hzW32gSyoFKxkdSM_kgUArUsuWnZJPse4g1xL2n4il7SpBBW0XZBfqynZzvpkTREP0eB-Lp1Nx8IPxajHUTurp2K0yZs_fuqD1a5IQU_R6WT99IVcDNpF_Ho-r8jv283zels-PN7dr1cPpWmqGsqqFaBxMF3Xad3Ufc16JlqKnRFy6LGWWmPNB-RcmgYlzZe9AC47YFwMhrEr8uOkuw_-7wFjUqPNdp3TE_pDVI2UFBoGb4JVpiSr6gzyE2iCjzHgoPbBjjocFQU1Z6x26pyxmjNW0Ob27OT7ecChG7H__-ocaga2J-DVOjy-T1Xdbm6qp3lh876AA63yf7LUz5MU5mhfLAYVjcXJYG8DmqR6b99w-w8IgqZx</recordid><startdate>20051121</startdate><enddate>20051121</enddate><creator>Zhang, Li</creator><creator>Ging, Ng Ching</creator><creator>Komoda, Taeko</creator><creator>Hanada, Takao</creator><creator>Suzuki, Tsutomu</creator><creator>Watanabe, Kimitsuna</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>20051121</creationdate><title>Antibiotic susceptibility of mammalian mitochondrial translation</title><author>Zhang, Li ; Ging, Ng Ching ; Komoda, Taeko ; Hanada, Takao ; Suzuki, Tsutomu ; Watanabe, Kimitsuna</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c6240-2970aefcbbbaa64d43d3791ebc78fde48aae45fe558c6e81379d7058b0357fc33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Animals</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Antibiotics</topic><topic>Cattle</topic><topic>Drug Resistance, Bacterial</topic><topic>E. coli</topic><topic>Escherichia coli</topic><topic>Escherichia coli - drug effects</topic><topic>Escherichia coli - genetics</topic><topic>Mitochondria</topic><topic>Mitochondria - drug effects</topic><topic>Mitochondria - metabolism</topic><topic>Peptide Elongation Factors - drug effects</topic><topic>Protein Biosynthesis - drug effects</topic><topic>Protein synthesis</topic><topic>Ribosome</topic><topic>Ribosomes - drug effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Li</creatorcontrib><creatorcontrib>Ging, Ng Ching</creatorcontrib><creatorcontrib>Komoda, Taeko</creatorcontrib><creatorcontrib>Hanada, Takao</creatorcontrib><creatorcontrib>Suzuki, Tsutomu</creatorcontrib><creatorcontrib>Watanabe, Kimitsuna</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Li</au><au>Ging, Ng Ching</au><au>Komoda, Taeko</au><au>Hanada, Takao</au><au>Suzuki, Tsutomu</au><au>Watanabe, Kimitsuna</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Antibiotic susceptibility of mammalian mitochondrial translation</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>2005-11-21</date><risdate>2005</risdate><volume>579</volume><issue>28</issue><spage>6423</spage><epage>6427</epage><pages>6423-6427</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><abstract>All medically useful antibiotics should have the potential to distinguish between target microbes (bacteria) and host cells. 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Escherichia coli and mitochondrial protein synthesis. The mitochondrial system was more resistant to tiamulin, macrolides, virginiamycin, fusidic acid and kirromycin than the
E. coli system. The present results, taken together with atomic structure of the ribosome, may provide useful information for the rational design of new antibiotics having less adverse effects in humans and animals.</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>16271719</pmid><doi>10.1016/j.febslet.2005.09.103</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Elsevier ScienceDirect Journals; Wiley Online Library Free Content; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Animals Anti-Bacterial Agents - pharmacology Antibiotics Cattle Drug Resistance, Bacterial E. coli Escherichia coli Escherichia coli - drug effects Escherichia coli - genetics Mitochondria Mitochondria - drug effects Mitochondria - metabolism Peptide Elongation Factors - drug effects Protein Biosynthesis - drug effects Protein synthesis Ribosome Ribosomes - drug effects |
| title | Antibiotic susceptibility of mammalian mitochondrial translation |
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