Molecular epidemiology of African swine fever in East Africa
African swine fever (ASF) a lethal, viral hemorrhagic disease of domestic pigs, first reported from East Africa in 1921, is still widespread in this region. In order to assess field heterogeneity at the regional level, nucleotide sequences corresponding to the C-terminal end of the p72 gene were det...
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| Veröffentlicht in: | Archives of virology 2005-12, Vol.150 (12), p.2439-2452 |
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| description | African swine fever (ASF) a lethal, viral hemorrhagic disease of domestic pigs, first reported from East Africa in 1921, is still widespread in this region. In order to assess field heterogeneity at the regional level, nucleotide sequences corresponding to the C-terminal end of the p72 gene were determined for 77 ASF viruses of diverse temporal and species origin occurring in eight East African countries. The number of sites completely conserved across all East African sequences characterized in this study was 84.2% and 86.8% on nucleotide and amino acid level, respectively. Phylogenetic analysis of a homologous 404 bp region revealed the presence of thirteen East African genotypes, of which eight appear to be country specific. An East African, pig-associated, homogeneous virus lineage linked to outbreaks in Mozambique, Zambia and Malawi over a 23 year period was demonstrated. In addition, genotype I (ESACWA) viruses were identified in East African sylvatic hosts for the first time which is significant as this genotype was previously thought to be restricted to the West African region where it occurs only in domestic pigs. The presence of discrete epidemiological cycles in East Africa and recovery of multiple genotypes affirms the epidemiological complexity of ASF in this region. |
| doi_str_mv | 10.1007/s00705-005-0602-1 |
| format | Article |
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A ; BASTOS, A. D. S ; DWARKA, R. M ; VOSLOO, W</creator><creatorcontrib>LUBISI, B. A ; BASTOS, A. D. S ; DWARKA, R. M ; VOSLOO, W</creatorcontrib><description>African swine fever (ASF) a lethal, viral hemorrhagic disease of domestic pigs, first reported from East Africa in 1921, is still widespread in this region. In order to assess field heterogeneity at the regional level, nucleotide sequences corresponding to the C-terminal end of the p72 gene were determined for 77 ASF viruses of diverse temporal and species origin occurring in eight East African countries. The number of sites completely conserved across all East African sequences characterized in this study was 84.2% and 86.8% on nucleotide and amino acid level, respectively. Phylogenetic analysis of a homologous 404 bp region revealed the presence of thirteen East African genotypes, of which eight appear to be country specific. An East African, pig-associated, homogeneous virus lineage linked to outbreaks in Mozambique, Zambia and Malawi over a 23 year period was demonstrated. In addition, genotype I (ESACWA) viruses were identified in East African sylvatic hosts for the first time which is significant as this genotype was previously thought to be restricted to the West African region where it occurs only in domestic pigs. The presence of discrete epidemiological cycles in East Africa and recovery of multiple genotypes affirms the epidemiological complexity of ASF in this region.</description><identifier>ISSN: 0304-8608</identifier><identifier>EISSN: 1432-8798</identifier><identifier>DOI: 10.1007/s00705-005-0602-1</identifier><identifier>PMID: 16052280</identifier><language>eng</language><publisher>Wien: Springer</publisher><subject>Africa, Eastern - epidemiology ; African swine fever ; African Swine Fever - epidemiology ; African Swine Fever - virology ; African swine fever virus ; Animals ; Asfarviridae - classification ; Asfarviridae - genetics ; Asfarviridae - isolation & purification ; Base Sequence ; Biological and medical sciences ; Capsid Proteins - genetics ; Conserved Sequence ; Disease ; DNA, Viral - chemistry ; DNA, Viral - genetics ; Epidemics ; Epidemiology ; Fundamental and applied biological sciences. Psychology ; Genotype ; Genotype & phenotype ; Geography ; Hogs ; Microbiology ; Miscellaneous ; Molecular Epidemiology ; Molecular Sequence Data ; Phylogeny ; Sequence Alignment ; Sequence Analysis, DNA ; Sus scrofa - virology ; Time Factors ; Virology ; Viruses</subject><ispartof>Archives of virology, 2005-12, Vol.150 (12), p.2439-2452</ispartof><rights>2006 INIST-CNRS</rights><rights>Springer-Verlag/Wien 2005</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c387t-7b818df6e77426bc2216957e95a737c903aef1d7e7f5efe79347e83ef4a174d93</citedby><cites>FETCH-LOGICAL-c387t-7b818df6e77426bc2216957e95a737c903aef1d7e7f5efe79347e83ef4a174d93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17313645$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16052280$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>LUBISI, B. A</creatorcontrib><creatorcontrib>BASTOS, A. D. S</creatorcontrib><creatorcontrib>DWARKA, R. M</creatorcontrib><creatorcontrib>VOSLOO, W</creatorcontrib><title>Molecular epidemiology of African swine fever in East Africa</title><title>Archives of virology</title><addtitle>Arch Virol</addtitle><description>African swine fever (ASF) a lethal, viral hemorrhagic disease of domestic pigs, first reported from East Africa in 1921, is still widespread in this region. In order to assess field heterogeneity at the regional level, nucleotide sequences corresponding to the C-terminal end of the p72 gene were determined for 77 ASF viruses of diverse temporal and species origin occurring in eight East African countries. The number of sites completely conserved across all East African sequences characterized in this study was 84.2% and 86.8% on nucleotide and amino acid level, respectively. Phylogenetic analysis of a homologous 404 bp region revealed the presence of thirteen East African genotypes, of which eight appear to be country specific. An East African, pig-associated, homogeneous virus lineage linked to outbreaks in Mozambique, Zambia and Malawi over a 23 year period was demonstrated. In addition, genotype I (ESACWA) viruses were identified in East African sylvatic hosts for the first time which is significant as this genotype was previously thought to be restricted to the West African region where it occurs only in domestic pigs. The presence of discrete epidemiological cycles in East Africa and recovery of multiple genotypes affirms the epidemiological complexity of ASF in this region.</description><subject>Africa, Eastern - epidemiology</subject><subject>African swine fever</subject><subject>African Swine Fever - epidemiology</subject><subject>African Swine Fever - virology</subject><subject>African swine fever virus</subject><subject>Animals</subject><subject>Asfarviridae - classification</subject><subject>Asfarviridae - genetics</subject><subject>Asfarviridae - isolation & purification</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Capsid Proteins - genetics</subject><subject>Conserved Sequence</subject><subject>Disease</subject><subject>DNA, Viral - chemistry</subject><subject>DNA, Viral - genetics</subject><subject>Epidemics</subject><subject>Epidemiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genotype</subject><subject>Genotype & phenotype</subject><subject>Geography</subject><subject>Hogs</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>Molecular Epidemiology</subject><subject>Molecular Sequence Data</subject><subject>Phylogeny</subject><subject>Sequence Alignment</subject><subject>Sequence Analysis, DNA</subject><subject>Sus scrofa - virology</subject><subject>Time Factors</subject><subject>Virology</subject><subject>Viruses</subject><issn>0304-8608</issn><issn>1432-8798</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFkU1LBDEMhosouq7-AC8yCHobTduZpgNeRNYPWPGi59LtpDIyH2u7o-y_d9YdELx4SALJkxeSl7ETDpccAK_ikCBPYRMKRMp32IRnUqQaC73LJiAhS7UCfcAOY3wHGBoy32cHXEEuhIYJu37qanJ9bUNCy6qkpurq7m2ddD658aFytk3iV9VS4umTQlK1yczG1Tg7Ynve1pGOxzplr3ezl9uHdP58_3h7M0-d1LhKcaG5Lr0ixEyohROCqyJHKnKLEl0B0pLnJRL6nDxhITMkLclnlmNWFnLKLra6y9B99BRXpqmio7q2LXV9NEprGBT1vyBHISX-gGd_wPeuD-1whBFcSKVzBQPEt5ALXYyBvFmGqrFhbTiYjQFma4CBTQwGGD7snI7C_aKh8ndj_PgAnI-Ajc7WPtjWVfGXQ8mlynL5DT61izY</recordid><startdate>20051201</startdate><enddate>20051201</enddate><creator>LUBISI, B. 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A ; BASTOS, A. D. S ; DWARKA, R. 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Psychology</topic><topic>Genotype</topic><topic>Genotype & phenotype</topic><topic>Geography</topic><topic>Hogs</topic><topic>Microbiology</topic><topic>Miscellaneous</topic><topic>Molecular Epidemiology</topic><topic>Molecular Sequence Data</topic><topic>Phylogeny</topic><topic>Sequence Alignment</topic><topic>Sequence Analysis, DNA</topic><topic>Sus scrofa - virology</topic><topic>Time Factors</topic><topic>Virology</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>LUBISI, B. A</creatorcontrib><creatorcontrib>BASTOS, A. D. S</creatorcontrib><creatorcontrib>DWARKA, R. 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A</au><au>BASTOS, A. D. S</au><au>DWARKA, R. M</au><au>VOSLOO, W</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular epidemiology of African swine fever in East Africa</atitle><jtitle>Archives of virology</jtitle><addtitle>Arch Virol</addtitle><date>2005-12-01</date><risdate>2005</risdate><volume>150</volume><issue>12</issue><spage>2439</spage><epage>2452</epage><pages>2439-2452</pages><issn>0304-8608</issn><eissn>1432-8798</eissn><abstract>African swine fever (ASF) a lethal, viral hemorrhagic disease of domestic pigs, first reported from East Africa in 1921, is still widespread in this region. In order to assess field heterogeneity at the regional level, nucleotide sequences corresponding to the C-terminal end of the p72 gene were determined for 77 ASF viruses of diverse temporal and species origin occurring in eight East African countries. The number of sites completely conserved across all East African sequences characterized in this study was 84.2% and 86.8% on nucleotide and amino acid level, respectively. Phylogenetic analysis of a homologous 404 bp region revealed the presence of thirteen East African genotypes, of which eight appear to be country specific. An East African, pig-associated, homogeneous virus lineage linked to outbreaks in Mozambique, Zambia and Malawi over a 23 year period was demonstrated. In addition, genotype I (ESACWA) viruses were identified in East African sylvatic hosts for the first time which is significant as this genotype was previously thought to be restricted to the West African region where it occurs only in domestic pigs. The presence of discrete epidemiological cycles in East Africa and recovery of multiple genotypes affirms the epidemiological complexity of ASF in this region.</abstract><cop>Wien</cop><cop>New York, NY</cop><pub>Springer</pub><pmid>16052280</pmid><doi>10.1007/s00705-005-0602-1</doi><tpages>14</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Africa, Eastern - epidemiology African swine fever African Swine Fever - epidemiology African Swine Fever - virology African swine fever virus Animals Asfarviridae - classification Asfarviridae - genetics Asfarviridae - isolation & purification Base Sequence Biological and medical sciences Capsid Proteins - genetics Conserved Sequence Disease DNA, Viral - chemistry DNA, Viral - genetics Epidemics Epidemiology Fundamental and applied biological sciences. Psychology Genotype Genotype & phenotype Geography Hogs Microbiology Miscellaneous Molecular Epidemiology Molecular Sequence Data Phylogeny Sequence Alignment Sequence Analysis, DNA Sus scrofa - virology Time Factors Virology Viruses |
| title | Molecular epidemiology of African swine fever in East Africa |
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