Leishmanin skin test in guinea pig with a single purified protein of Leishmania major

A series of hybridomas was produced by fusion of SP2/0 myeloma cells with spleen cells of mice immunized with Leishmania major ( L. major). The reactivity of secreted monoclonal antibodies (mAbs) was evaluated against available leishmanin antigen by enzyme linked immunosorbent assay. Only one hybrid...

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Veröffentlicht in:	Experimental parasitology 2005-12, Vol.111 (4), p.239-243
Hauptverfasser:	Khabiri, A.R., Bagheri, F., Alimohammadian, M.H., Assmar, M., Nadaf, S.R.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antibodies, Monoclonal - immunology Antigens, Protozoan - immunology Antigens, Protozoan - isolation & purification Biological and medical sciences Blotting, Western Chromatography, Affinity Chromatography, High Pressure Liquid Delayed-type hypersensitivity Electrophoresis, Polyacrylamide Gel Enzyme-Linked Immunosorbent Assay Female Fundamental and applied biological sciences. Psychology Guinea Pigs Hybridomas Hypersensitivity, Delayed Intradermal Tests - standards Leishmania major Leishmania major - immunology Leishmaniasis - diagnosis Leishmaniasis - immunology Leishmanin Life cycle. Host-agent relationship. Pathogenesis Mammalia Mice Mice, Inbred BALB C Monoclonal antibody Protozoa Protozoan Proteins - immunology Protozoan Proteins - isolation & purification Single antigen Vertebrates: general zoology, morphology, phylogeny, systematics, cytogenetics, geographical distribution
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