Molecular characterization of Mycobacterium tuberculosis isolates in a region of Brazil with a high incidence of tuberculosis
One hundred and seventy Mycobacterium tuberculosis clinical isolates were characterized by spoligotyping to evaluate the biodiversity of tubercle bacilli in a region of Brazil with a high incidence of tuberculosis (Pelotas and Rio Grande cities – Rio Grande do Sul State). The spoligotyping results w...
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| Veröffentlicht in: | Microbes and infection 2005-10, Vol.7 (13), p.1338-1344 |
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| creator | Borsuk, Sibele Dellagostin, Marina Mirian Madeira, Suselaine de Góes Lima, Crislaine Boffo, Marta Mattos, Ivo Almeida da Silva, Pedro E. Dellagostin, Odir Antônio |
| description | One hundred and seventy
Mycobacterium tuberculosis clinical isolates were characterized by spoligotyping to evaluate the biodiversity of tubercle bacilli in a region of Brazil with a high incidence of tuberculosis (Pelotas and Rio Grande cities – Rio Grande do Sul State). The spoligotyping results were compared to the World Spoligotyping Database (Institut Pasteur de Guadeloupe), which contains data from >
14,000 worldwide isolates of
M. tuberculosis. The isolates clustered by spoligotyping were further characterized by IS6110-RFLP to confirm the clonal relationship. Sixty-six different spoligotypes were identified, grouping 125 of the isolates (74%). Approximately half of the isolates belonged to seven of the most frequently occurring spoligotypes in the database. Three shared types (with two or more isolates) not previously identified were given the type numbers 826, 827 and 863. An additional 45 spoligotypes were identified that did not match any existing database pattern. RFLP characterization reduced the number of isolates in most of the clusters, thereby showing a higher differentiation capacity than spoligotyping. These results highlight the importance of molecular epidemiology studies of tuberculosis in insufficiently studied regions with a high TB burden, in order to uncover the true extent of genetic diversity of the pathogen. |
| doi_str_mv | 10.1016/j.micinf.2005.05.009 |
| format | Article |
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Mycobacterium tuberculosis clinical isolates were characterized by spoligotyping to evaluate the biodiversity of tubercle bacilli in a region of Brazil with a high incidence of tuberculosis (Pelotas and Rio Grande cities – Rio Grande do Sul State). The spoligotyping results were compared to the World Spoligotyping Database (Institut Pasteur de Guadeloupe), which contains data from >
14,000 worldwide isolates of
M. tuberculosis. The isolates clustered by spoligotyping were further characterized by IS6110-RFLP to confirm the clonal relationship. Sixty-six different spoligotypes were identified, grouping 125 of the isolates (74%). Approximately half of the isolates belonged to seven of the most frequently occurring spoligotypes in the database. Three shared types (with two or more isolates) not previously identified were given the type numbers 826, 827 and 863. An additional 45 spoligotypes were identified that did not match any existing database pattern. RFLP characterization reduced the number of isolates in most of the clusters, thereby showing a higher differentiation capacity than spoligotyping. These results highlight the importance of molecular epidemiology studies of tuberculosis in insufficiently studied regions with a high TB burden, in order to uncover the true extent of genetic diversity of the pathogen.</description><identifier>ISSN: 1286-4579</identifier><identifier>EISSN: 1769-714X</identifier><identifier>DOI: 10.1016/j.micinf.2005.05.009</identifier><identifier>PMID: 16039895</identifier><language>eng</language><publisher>Lausanne: Elsevier SAS</publisher><subject>Bacterial diseases ; Bacterial Typing Techniques ; Biological and medical sciences ; Brazil - epidemiology ; DNA Transposable Elements ; DNA, Bacterial - analysis ; Fundamental and applied biological sciences. Psychology ; Human bacterial diseases ; Humans ; Incidence ; Infectious diseases ; IS 6110-RFLP ; Medical sciences ; Microbiology ; Molecular Epidemiology ; Mycobacterium tuberculosis ; Mycobacterium tuberculosis - classification ; Mycobacterium tuberculosis - genetics ; Mycobacterium tuberculosis - isolation & purification ; Polymorphism, Restriction Fragment Length ; Spoligotyping ; Tuberculosis - epidemiology ; Tuberculosis - microbiology ; Tuberculosis and atypical mycobacterial infections</subject><ispartof>Microbes and infection, 2005-10, Vol.7 (13), p.1338-1344</ispartof><rights>2005 Elsevier SAS</rights><rights>2006 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c467t-a775df192b83766ee3bd052efb9902f73fa102509bf378152dad5bc5cb2acffc3</citedby><cites>FETCH-LOGICAL-c467t-a775df192b83766ee3bd052efb9902f73fa102509bf378152dad5bc5cb2acffc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.micinf.2005.05.009$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17258104$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16039895$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Borsuk, Sibele</creatorcontrib><creatorcontrib>Dellagostin, Marina Mirian</creatorcontrib><creatorcontrib>Madeira, Suselaine de Góes</creatorcontrib><creatorcontrib>Lima, Crislaine</creatorcontrib><creatorcontrib>Boffo, Marta</creatorcontrib><creatorcontrib>Mattos, Ivo</creatorcontrib><creatorcontrib>Almeida da Silva, Pedro E.</creatorcontrib><creatorcontrib>Dellagostin, Odir Antônio</creatorcontrib><title>Molecular characterization of Mycobacterium tuberculosis isolates in a region of Brazil with a high incidence of tuberculosis</title><title>Microbes and infection</title><addtitle>Microbes Infect</addtitle><description>One hundred and seventy
Mycobacterium tuberculosis clinical isolates were characterized by spoligotyping to evaluate the biodiversity of tubercle bacilli in a region of Brazil with a high incidence of tuberculosis (Pelotas and Rio Grande cities – Rio Grande do Sul State). The spoligotyping results were compared to the World Spoligotyping Database (Institut Pasteur de Guadeloupe), which contains data from >
14,000 worldwide isolates of
M. tuberculosis. The isolates clustered by spoligotyping were further characterized by IS6110-RFLP to confirm the clonal relationship. Sixty-six different spoligotypes were identified, grouping 125 of the isolates (74%). Approximately half of the isolates belonged to seven of the most frequently occurring spoligotypes in the database. Three shared types (with two or more isolates) not previously identified were given the type numbers 826, 827 and 863. An additional 45 spoligotypes were identified that did not match any existing database pattern. RFLP characterization reduced the number of isolates in most of the clusters, thereby showing a higher differentiation capacity than spoligotyping. These results highlight the importance of molecular epidemiology studies of tuberculosis in insufficiently studied regions with a high TB burden, in order to uncover the true extent of genetic diversity of the pathogen.</description><subject>Bacterial diseases</subject><subject>Bacterial Typing Techniques</subject><subject>Biological and medical sciences</subject><subject>Brazil - epidemiology</subject><subject>DNA Transposable Elements</subject><subject>DNA, Bacterial - analysis</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Human bacterial diseases</subject><subject>Humans</subject><subject>Incidence</subject><subject>Infectious diseases</subject><subject>IS 6110-RFLP</subject><subject>Medical sciences</subject><subject>Microbiology</subject><subject>Molecular Epidemiology</subject><subject>Mycobacterium tuberculosis</subject><subject>Mycobacterium tuberculosis - classification</subject><subject>Mycobacterium tuberculosis - genetics</subject><subject>Mycobacterium tuberculosis - isolation & purification</subject><subject>Polymorphism, Restriction Fragment Length</subject><subject>Spoligotyping</subject><subject>Tuberculosis - epidemiology</subject><subject>Tuberculosis - microbiology</subject><subject>Tuberculosis and atypical mycobacterial infections</subject><issn>1286-4579</issn><issn>1769-714X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkV9r1jAUxoso7o9-A5He6F1fT9ImaW4EHU6Fjd1M8C4k6cnevLTNTFplg313U1qYVw4O5HDyex4O5ymKNwR2BAj_cNgN3vrR7SgA2y0F8llxTASXlSDNz-e5py2vGibkUXGS0gGAMMGbl8UR4VDLVrLj4uEy9GjnXsfS7nXUdsLo7_Xkw1gGV17e2WDW4TyU02wwZjgkn0qfQq8nzM1Y6jLizSb5HPW978s_ftrn-d7f7DNhfYejxeX_X5NXxQun-4Svt_e0-HH-5frsW3Vx9fX72aeLyjZcTJUWgnWOSGraWnCOWJsOGEVnpATqRO00AcpAGleLljDa6Y4Zy6yh2jpn69Pi_ep7G8OvGdOkBp8s9r0eMcxJ8baFRgr-JEhBshpakcFmBW0MKUV06jb6Qcc7RUAt-aiDWvNRSz5qKZBZ9nbzn82A3aNoCyQD7zZAJ6t7F3W-XXrkBGUtgSZzH1cO89l-e4wqWb-cuPMR7aS64P-_yV9ZP7NN</recordid><startdate>20051001</startdate><enddate>20051001</enddate><creator>Borsuk, Sibele</creator><creator>Dellagostin, Marina Mirian</creator><creator>Madeira, Suselaine de Góes</creator><creator>Lima, Crislaine</creator><creator>Boffo, Marta</creator><creator>Mattos, Ivo</creator><creator>Almeida da Silva, Pedro E.</creator><creator>Dellagostin, Odir Antônio</creator><general>Elsevier SAS</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>20051001</creationdate><title>Molecular characterization of Mycobacterium tuberculosis isolates in a region of Brazil with a high incidence of tuberculosis</title><author>Borsuk, Sibele ; Dellagostin, Marina Mirian ; Madeira, Suselaine de Góes ; Lima, Crislaine ; Boffo, Marta ; Mattos, Ivo ; Almeida da Silva, Pedro E. ; Dellagostin, Odir Antônio</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c467t-a775df192b83766ee3bd052efb9902f73fa102509bf378152dad5bc5cb2acffc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Bacterial diseases</topic><topic>Bacterial Typing Techniques</topic><topic>Biological and medical sciences</topic><topic>Brazil - epidemiology</topic><topic>DNA Transposable Elements</topic><topic>DNA, Bacterial - analysis</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Human bacterial diseases</topic><topic>Humans</topic><topic>Incidence</topic><topic>Infectious diseases</topic><topic>IS 6110-RFLP</topic><topic>Medical sciences</topic><topic>Microbiology</topic><topic>Molecular Epidemiology</topic><topic>Mycobacterium tuberculosis</topic><topic>Mycobacterium tuberculosis - classification</topic><topic>Mycobacterium tuberculosis - genetics</topic><topic>Mycobacterium tuberculosis - isolation & purification</topic><topic>Polymorphism, Restriction Fragment Length</topic><topic>Spoligotyping</topic><topic>Tuberculosis - epidemiology</topic><topic>Tuberculosis - microbiology</topic><topic>Tuberculosis and atypical mycobacterial infections</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Borsuk, Sibele</creatorcontrib><creatorcontrib>Dellagostin, Marina Mirian</creatorcontrib><creatorcontrib>Madeira, Suselaine de Góes</creatorcontrib><creatorcontrib>Lima, Crislaine</creatorcontrib><creatorcontrib>Boffo, Marta</creatorcontrib><creatorcontrib>Mattos, Ivo</creatorcontrib><creatorcontrib>Almeida da Silva, Pedro E.</creatorcontrib><creatorcontrib>Dellagostin, Odir Antônio</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Microbes and infection</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Borsuk, Sibele</au><au>Dellagostin, Marina Mirian</au><au>Madeira, Suselaine de Góes</au><au>Lima, Crislaine</au><au>Boffo, Marta</au><au>Mattos, Ivo</au><au>Almeida da Silva, Pedro E.</au><au>Dellagostin, Odir Antônio</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular characterization of Mycobacterium tuberculosis isolates in a region of Brazil with a high incidence of tuberculosis</atitle><jtitle>Microbes and infection</jtitle><addtitle>Microbes Infect</addtitle><date>2005-10-01</date><risdate>2005</risdate><volume>7</volume><issue>13</issue><spage>1338</spage><epage>1344</epage><pages>1338-1344</pages><issn>1286-4579</issn><eissn>1769-714X</eissn><abstract>One hundred and seventy
Mycobacterium tuberculosis clinical isolates were characterized by spoligotyping to evaluate the biodiversity of tubercle bacilli in a region of Brazil with a high incidence of tuberculosis (Pelotas and Rio Grande cities – Rio Grande do Sul State). The spoligotyping results were compared to the World Spoligotyping Database (Institut Pasteur de Guadeloupe), which contains data from >
14,000 worldwide isolates of
M. tuberculosis. The isolates clustered by spoligotyping were further characterized by IS6110-RFLP to confirm the clonal relationship. Sixty-six different spoligotypes were identified, grouping 125 of the isolates (74%). Approximately half of the isolates belonged to seven of the most frequently occurring spoligotypes in the database. Three shared types (with two or more isolates) not previously identified were given the type numbers 826, 827 and 863. An additional 45 spoligotypes were identified that did not match any existing database pattern. RFLP characterization reduced the number of isolates in most of the clusters, thereby showing a higher differentiation capacity than spoligotyping. These results highlight the importance of molecular epidemiology studies of tuberculosis in insufficiently studied regions with a high TB burden, in order to uncover the true extent of genetic diversity of the pathogen.</abstract><cop>Lausanne</cop><cop>Amsterdam</cop><cop>Paris</cop><pub>Elsevier SAS</pub><pmid>16039895</pmid><doi>10.1016/j.micinf.2005.05.009</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Microbes and infection, 2005-10, Vol.7 (13), p.1338-1344 |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Bacterial diseases Bacterial Typing Techniques Biological and medical sciences Brazil - epidemiology DNA Transposable Elements DNA, Bacterial - analysis Fundamental and applied biological sciences. Psychology Human bacterial diseases Humans Incidence Infectious diseases IS 6110-RFLP Medical sciences Microbiology Molecular Epidemiology Mycobacterium tuberculosis Mycobacterium tuberculosis - classification Mycobacterium tuberculosis - genetics Mycobacterium tuberculosis - isolation & purification Polymorphism, Restriction Fragment Length Spoligotyping Tuberculosis - epidemiology Tuberculosis - microbiology Tuberculosis and atypical mycobacterial infections |
| title | Molecular characterization of Mycobacterium tuberculosis isolates in a region of Brazil with a high incidence of tuberculosis |
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