Oligosaccharides produced by submerged cultures of Claviceps africana and Claviceps sorghi
Oligosaccharides produced by submerged cultures of C. africana and C. sorghi were isolated by semipreparative HPLC. Structure of 6-O-beta-D-fructofuranosyl-D-glucopyranose (blastose), 1,6-bis-O-(beta-D-fructofuranosyl)-alpha-D-glucopyranoside (neokestose) and two sugar alcohols, 1-O-beta-D-fructofur...
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| Veröffentlicht in: | Folia microbiologica 2005, Vol.50 (3), p.198-204 |
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| container_title | Folia microbiologica |
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| creator | Flieger, M Kantorová, M Halada, P Kuzma, M Pazoutová, S Stodůlková, E Kolínská, R |
| description | Oligosaccharides produced by submerged cultures of C. africana and C. sorghi were isolated by semipreparative HPLC. Structure of 6-O-beta-D-fructofuranosyl-D-glucopyranose (blastose), 1,6-bis-O-(beta-D-fructofuranosyl)-alpha-D-glucopyranoside (neokestose) and two sugar alcohols, 1-O-beta-D-fructofuranosyl-D-mannitol (fructosylmannitol) and 1,6-bis-O-(beta-D-fructofuranosyl)-D-mannitol (bisfructosylmannitol) was determined by NMR spectrometry. MALDI TOF MS analysis revealed molecular ions [M+Na]+ that indicate the presence of other tetra- and pentasaccharides (m/z = 689.4 and 851.5, respectively) and corresponding sugar alcohol (m/z = 691.4). Rapid conversion of sucrose into series of oligosaccharides and corresponding sugar alcohols was observed in all tested strains. |
| doi_str_mv | 10.1007/BF02931566 |
| format | Article |
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Structure of 6-O-beta-D-fructofuranosyl-D-glucopyranose (blastose), 1,6-bis-O-(beta-D-fructofuranosyl)-alpha-D-glucopyranoside (neokestose) and two sugar alcohols, 1-O-beta-D-fructofuranosyl-D-mannitol (fructosylmannitol) and 1,6-bis-O-(beta-D-fructofuranosyl)-D-mannitol (bisfructosylmannitol) was determined by NMR spectrometry. MALDI TOF MS analysis revealed molecular ions [M+Na]+ that indicate the presence of other tetra- and pentasaccharides (m/z = 689.4 and 851.5, respectively) and corresponding sugar alcohol (m/z = 691.4). Rapid conversion of sucrose into series of oligosaccharides and corresponding sugar alcohols was observed in all tested strains.</description><identifier>ISSN: 0015-5632</identifier><identifier>DOI: 10.1007/BF02931566</identifier><identifier>PMID: 16295657</identifier><language>eng</language><publisher>United States</publisher><subject>Chromatography, High Pressure Liquid ; Claviceps ; Claviceps - growth & development ; Claviceps - isolation & purification ; Claviceps - metabolism ; Claviceps - pathogenicity ; Claviceps africana ; Fermentation ; Molecular Structure ; Nuclear Magnetic Resonance, Biomolecular ; Oligosaccharides - biosynthesis ; Oligosaccharides - chemistry ; Oligosaccharides - isolation & purification ; Plant Diseases - microbiology ; Sorghum - microbiology ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ; Sugar Alcohols - chemistry ; Sugar Alcohols - metabolism</subject><ispartof>Folia microbiologica, 2005, Vol.50 (3), p.198-204</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,4009,27902,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16295657$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Flieger, M</creatorcontrib><creatorcontrib>Kantorová, M</creatorcontrib><creatorcontrib>Halada, P</creatorcontrib><creatorcontrib>Kuzma, M</creatorcontrib><creatorcontrib>Pazoutová, S</creatorcontrib><creatorcontrib>Stodůlková, E</creatorcontrib><creatorcontrib>Kolínská, R</creatorcontrib><title>Oligosaccharides produced by submerged cultures of Claviceps africana and Claviceps sorghi</title><title>Folia microbiologica</title><addtitle>Folia Microbiol (Praha)</addtitle><description>Oligosaccharides produced by submerged cultures of C. africana and C. sorghi were isolated by semipreparative HPLC. Structure of 6-O-beta-D-fructofuranosyl-D-glucopyranose (blastose), 1,6-bis-O-(beta-D-fructofuranosyl)-alpha-D-glucopyranoside (neokestose) and two sugar alcohols, 1-O-beta-D-fructofuranosyl-D-mannitol (fructosylmannitol) and 1,6-bis-O-(beta-D-fructofuranosyl)-D-mannitol (bisfructosylmannitol) was determined by NMR spectrometry. MALDI TOF MS analysis revealed molecular ions [M+Na]+ that indicate the presence of other tetra- and pentasaccharides (m/z = 689.4 and 851.5, respectively) and corresponding sugar alcohol (m/z = 691.4). Rapid conversion of sucrose into series of oligosaccharides and corresponding sugar alcohols was observed in all tested strains.</description><subject>Chromatography, High Pressure Liquid</subject><subject>Claviceps</subject><subject>Claviceps - growth & development</subject><subject>Claviceps - isolation & purification</subject><subject>Claviceps - metabolism</subject><subject>Claviceps - pathogenicity</subject><subject>Claviceps africana</subject><subject>Fermentation</subject><subject>Molecular Structure</subject><subject>Nuclear Magnetic Resonance, Biomolecular</subject><subject>Oligosaccharides - biosynthesis</subject><subject>Oligosaccharides - chemistry</subject><subject>Oligosaccharides - isolation & purification</subject><subject>Plant Diseases - microbiology</subject><subject>Sorghum - microbiology</subject><subject>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</subject><subject>Sugar Alcohols - chemistry</subject><subject>Sugar Alcohols - metabolism</subject><issn>0015-5632</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1Lw0AQhveg2Fq9-AMkJ2_R_f44arEqFHrRi5cw2d2kK2kTdxOh_94EK3gTBl5m5mF450XoiuBbgrG6e1hhahgRUp6gOcZE5EIyOkPnKX1gLDFn9AzNiKRGSKHm6H3ThLpNYO0WYnA-ZV1s3WC9y8pDloZy52M9NnZo-iGO67bKlg18Beu7lEEVg4U9ZLB3f8apjfU2XKDTCprkL4-6QG-rx9flc77ePL0s79d5RznucyNLUwGxGBh1jnqOrZPKWm8BrKrAkVJpbSjXHAtqlNJiKke8MxXXlC3Qzc_d0fnn4FNf7EKyvmlg79shFVLrKRP1L0gMJ1RSNoLXR3D63xVdDDuIh-I3NvYNxbttBQ</recordid><startdate>2005</startdate><enddate>2005</enddate><creator>Flieger, M</creator><creator>Kantorová, M</creator><creator>Halada, P</creator><creator>Kuzma, M</creator><creator>Pazoutová, S</creator><creator>Stodůlková, E</creator><creator>Kolínská, R</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>F1W</scope><scope>FR3</scope><scope>H95</scope><scope>L.G</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>2005</creationdate><title>Oligosaccharides produced by submerged cultures of Claviceps africana and Claviceps sorghi</title><author>Flieger, M ; 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Structure of 6-O-beta-D-fructofuranosyl-D-glucopyranose (blastose), 1,6-bis-O-(beta-D-fructofuranosyl)-alpha-D-glucopyranoside (neokestose) and two sugar alcohols, 1-O-beta-D-fructofuranosyl-D-mannitol (fructosylmannitol) and 1,6-bis-O-(beta-D-fructofuranosyl)-D-mannitol (bisfructosylmannitol) was determined by NMR spectrometry. MALDI TOF MS analysis revealed molecular ions [M+Na]+ that indicate the presence of other tetra- and pentasaccharides (m/z = 689.4 and 851.5, respectively) and corresponding sugar alcohol (m/z = 691.4). Rapid conversion of sucrose into series of oligosaccharides and corresponding sugar alcohols was observed in all tested strains.</abstract><cop>United States</cop><pmid>16295657</pmid><doi>10.1007/BF02931566</doi><tpages>7</tpages></addata></record> |
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| subjects | Chromatography, High Pressure Liquid Claviceps Claviceps - growth & development Claviceps - isolation & purification Claviceps - metabolism Claviceps - pathogenicity Claviceps africana Fermentation Molecular Structure Nuclear Magnetic Resonance, Biomolecular Oligosaccharides - biosynthesis Oligosaccharides - chemistry Oligosaccharides - isolation & purification Plant Diseases - microbiology Sorghum - microbiology Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Sugar Alcohols - chemistry Sugar Alcohols - metabolism |
| title | Oligosaccharides produced by submerged cultures of Claviceps africana and Claviceps sorghi |
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