Hypothalamic ghrelin treatment modulates NPY-but not CRH-ergic activity in adrenalectomized rats subjected to food restriction: Evidence of a novel hypothalamic ghrelin effect
It has been proposed that ghrelin induces food intake by a mechanism due to the stimulation of hypothalamic NPY-ergic activity. It is recognized that bilateral adrenalectomy (ADX) enhances hypothalamic CRH-ergic function and reduces appetite. Thus, the aim of the present study was to test whether, i...
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| Veröffentlicht in: | Endocrine 2006-06, Vol.29 (3), p.477-484 |
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| creator | Spinedi, Eduardo Voirol, Marie-Jeanne Verdumo, Chantal Giacominni, Marco Pralong, François Gaillard, Rolf C |
| description | It has been proposed that ghrelin induces food intake by a mechanism due to the stimulation of hypothalamic NPY-ergic activity. It is recognized that bilateral adrenalectomy (ADX) enhances hypothalamic CRH-ergic function and reduces appetite. Thus, the aim of the present study was to test whether, icv-administered, ghrelin modulates NPY- and CRH-ergic functions after food restriction (FR) and glucocorticoid deprivation. For this purpose, 1 microg ghrelin was administered icv to ad libitum (AL) eating and to corticosterone (B)-depleted (ADX) and -replete (sham and ADX+B) male animals habituated, for 15 d, to FR. Food intake, hypothalamic function, and peripheral ghrelin, ACTH, and B concentrations were evaluated 2 h after ghrelin administration. Results indicate that while icv ghrelin treatment stimulated 2-h food intake in AL rats, it failed to do so in sham- and ADX+B-FR animals; moreover, 2-h food intake was inhibited by icv ghrelin treatment in ADX-FR rats. Regarding peripheral hormone levels: (a) basal circulating ghrelin levels, already enhanced (vs AL rats) by FR, significantly increased 2 h after icv ghrelin treatment in AL and sham-FR rats; (b) central ghrelin treatment stimulated ACTH secretion in circulation of AL and glucocorticoid-replete-FR rats; and (c) B circulating levels remained unchanged after ghrelin treatment, although they were in relation to the food intake condition of rats. Finally, hypothalamic NPY mRNA expression was enhanced by FR and, in response to icv ghrelin treatment, it decreased in ADX-FR rats only. ADX-enhanced hypothalamic CRH mRNA levels were reduced by ghrelin icv administration only when animals received B replacement therapy. Our data indicate an inhibitory effect of hypothalamic ghrelin on NPY-ergic activity in FR rats lacking endogenous glucocorticoid. |
| doi_str_mv | 10.1385/ENDO:29:3:477 |
| format | Article |
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It is recognized that bilateral adrenalectomy (ADX) enhances hypothalamic CRH-ergic function and reduces appetite. Thus, the aim of the present study was to test whether, icv-administered, ghrelin modulates NPY- and CRH-ergic functions after food restriction (FR) and glucocorticoid deprivation. For this purpose, 1 microg ghrelin was administered icv to ad libitum (AL) eating and to corticosterone (B)-depleted (ADX) and -replete (sham and ADX+B) male animals habituated, for 15 d, to FR. Food intake, hypothalamic function, and peripheral ghrelin, ACTH, and B concentrations were evaluated 2 h after ghrelin administration. Results indicate that while icv ghrelin treatment stimulated 2-h food intake in AL rats, it failed to do so in sham- and ADX+B-FR animals; moreover, 2-h food intake was inhibited by icv ghrelin treatment in ADX-FR rats. Regarding peripheral hormone levels: (a) basal circulating ghrelin levels, already enhanced (vs AL rats) by FR, significantly increased 2 h after icv ghrelin treatment in AL and sham-FR rats; (b) central ghrelin treatment stimulated ACTH secretion in circulation of AL and glucocorticoid-replete-FR rats; and (c) B circulating levels remained unchanged after ghrelin treatment, although they were in relation to the food intake condition of rats. Finally, hypothalamic NPY mRNA expression was enhanced by FR and, in response to icv ghrelin treatment, it decreased in ADX-FR rats only. ADX-enhanced hypothalamic CRH mRNA levels were reduced by ghrelin icv administration only when animals received B replacement therapy. Our data indicate an inhibitory effect of hypothalamic ghrelin on NPY-ergic activity in FR rats lacking endogenous glucocorticoid.</description><identifier>ISSN: 1355-008X</identifier><identifier>ISSN: 0969-711X</identifier><identifier>EISSN: 0969-711X</identifier><identifier>DOI: 10.1385/ENDO:29:3:477</identifier><identifier>PMID: 16943587</identifier><language>eng</language><publisher>United States</publisher><subject>Adrenalectomy ; Adrenocorticotropic Hormone - blood ; Animals ; Body Weight ; Corticotropin-Releasing Hormone - metabolism ; Drug Administration Routes ; Eating - drug effects ; Food Deprivation - physiology ; Gene Expression - drug effects ; Ghrelin ; Glucocorticoids - physiology ; Hypothalamus - drug effects ; Hypothalamus - metabolism ; Injections - methods ; Leptin - blood ; Male ; Neuropeptide Y - metabolism ; Peptide Hormones - administration & dosage ; Peptide Hormones - blood ; Peptide Hormones - pharmacology ; Rats ; Rats, Wistar ; RNA, Messenger - metabolism</subject><ispartof>Endocrine, 2006-06, Vol.29 (3), p.477-484</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c330t-6a0f2e8d7726590604dfa3854f60747d2c68ba05a64fa0d2ab7975392aeb51b23</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,27929,27930</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16943587$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Spinedi, Eduardo</creatorcontrib><creatorcontrib>Voirol, Marie-Jeanne</creatorcontrib><creatorcontrib>Verdumo, Chantal</creatorcontrib><creatorcontrib>Giacominni, Marco</creatorcontrib><creatorcontrib>Pralong, François</creatorcontrib><creatorcontrib>Gaillard, Rolf C</creatorcontrib><title>Hypothalamic ghrelin treatment modulates NPY-but not CRH-ergic activity in adrenalectomized rats subjected to food restriction: Evidence of a novel hypothalamic ghrelin effect</title><title>Endocrine</title><addtitle>Endocrine</addtitle><description>It has been proposed that ghrelin induces food intake by a mechanism due to the stimulation of hypothalamic NPY-ergic activity. It is recognized that bilateral adrenalectomy (ADX) enhances hypothalamic CRH-ergic function and reduces appetite. Thus, the aim of the present study was to test whether, icv-administered, ghrelin modulates NPY- and CRH-ergic functions after food restriction (FR) and glucocorticoid deprivation. For this purpose, 1 microg ghrelin was administered icv to ad libitum (AL) eating and to corticosterone (B)-depleted (ADX) and -replete (sham and ADX+B) male animals habituated, for 15 d, to FR. Food intake, hypothalamic function, and peripheral ghrelin, ACTH, and B concentrations were evaluated 2 h after ghrelin administration. Results indicate that while icv ghrelin treatment stimulated 2-h food intake in AL rats, it failed to do so in sham- and ADX+B-FR animals; moreover, 2-h food intake was inhibited by icv ghrelin treatment in ADX-FR rats. Regarding peripheral hormone levels: (a) basal circulating ghrelin levels, already enhanced (vs AL rats) by FR, significantly increased 2 h after icv ghrelin treatment in AL and sham-FR rats; (b) central ghrelin treatment stimulated ACTH secretion in circulation of AL and glucocorticoid-replete-FR rats; and (c) B circulating levels remained unchanged after ghrelin treatment, although they were in relation to the food intake condition of rats. Finally, hypothalamic NPY mRNA expression was enhanced by FR and, in response to icv ghrelin treatment, it decreased in ADX-FR rats only. ADX-enhanced hypothalamic CRH mRNA levels were reduced by ghrelin icv administration only when animals received B replacement therapy. Our data indicate an inhibitory effect of hypothalamic ghrelin on NPY-ergic activity in FR rats lacking endogenous glucocorticoid.</description><subject>Adrenalectomy</subject><subject>Adrenocorticotropic Hormone - blood</subject><subject>Animals</subject><subject>Body Weight</subject><subject>Corticotropin-Releasing Hormone - metabolism</subject><subject>Drug Administration Routes</subject><subject>Eating - drug effects</subject><subject>Food Deprivation - physiology</subject><subject>Gene Expression - drug effects</subject><subject>Ghrelin</subject><subject>Glucocorticoids - physiology</subject><subject>Hypothalamus - drug effects</subject><subject>Hypothalamus - metabolism</subject><subject>Injections - methods</subject><subject>Leptin - blood</subject><subject>Male</subject><subject>Neuropeptide Y - metabolism</subject><subject>Peptide Hormones - administration & dosage</subject><subject>Peptide Hormones - blood</subject><subject>Peptide Hormones - pharmacology</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>RNA, Messenger - metabolism</subject><issn>1355-008X</issn><issn>0969-711X</issn><issn>0969-711X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkU-LFDEQxYMo7rh69Co5eWvNn07SPTcZR0dYdkUU1lOo7q7sZOnujEl6YPxSfkWz7IAXT0UV7z0e9SPkNWfvuGzU--31x5u1aNdyXRvzhKxYq9vKcH77lKy4VKpirLm9IC9SumdMCKHNc3LBdVtL1ZgV-bM7HULewwiT7-ndPuLoZ5ojQp5wznQKwzJCxkSvv_6suiXTOWS6-barMN4VB_TZH30-0eKCIeIMI_Y5TP43DjRCTjQt3X05lTUH6kIoZ0w5-mIM85puj37AuUcaHIUSfsSR7v_XCZ0rMS_JMwdjwlfneUl-fNp-3-yqq5vPXzYfrqpeSpYrDcwJbAZjhFYt06weHJR31U4zU5tB9LrpgCnQtQM2COhMa5RsBWCneCfkJXn7mHuI4ddSCtvJpx7HEWYMS7K6aRhT3BRh9SjsY0gporOH6CeIJ8uZfSBkHwhZ0VppC6Gif3MOXroJh3_qMxL5F_eEkII</recordid><startdate>20060601</startdate><enddate>20060601</enddate><creator>Spinedi, Eduardo</creator><creator>Voirol, Marie-Jeanne</creator><creator>Verdumo, Chantal</creator><creator>Giacominni, Marco</creator><creator>Pralong, François</creator><creator>Gaillard, Rolf C</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20060601</creationdate><title>Hypothalamic ghrelin treatment modulates NPY-but not CRH-ergic activity in adrenalectomized rats subjected to food restriction: Evidence of a novel hypothalamic ghrelin effect</title><author>Spinedi, Eduardo ; Voirol, Marie-Jeanne ; Verdumo, Chantal ; Giacominni, Marco ; Pralong, François ; Gaillard, Rolf C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c330t-6a0f2e8d7726590604dfa3854f60747d2c68ba05a64fa0d2ab7975392aeb51b23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Adrenalectomy</topic><topic>Adrenocorticotropic Hormone - blood</topic><topic>Animals</topic><topic>Body Weight</topic><topic>Corticotropin-Releasing Hormone - metabolism</topic><topic>Drug Administration Routes</topic><topic>Eating - drug effects</topic><topic>Food Deprivation - physiology</topic><topic>Gene Expression - drug effects</topic><topic>Ghrelin</topic><topic>Glucocorticoids - physiology</topic><topic>Hypothalamus - drug effects</topic><topic>Hypothalamus - metabolism</topic><topic>Injections - methods</topic><topic>Leptin - blood</topic><topic>Male</topic><topic>Neuropeptide Y - metabolism</topic><topic>Peptide Hormones - administration & dosage</topic><topic>Peptide Hormones - blood</topic><topic>Peptide Hormones - pharmacology</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>RNA, Messenger - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Spinedi, Eduardo</creatorcontrib><creatorcontrib>Voirol, Marie-Jeanne</creatorcontrib><creatorcontrib>Verdumo, Chantal</creatorcontrib><creatorcontrib>Giacominni, Marco</creatorcontrib><creatorcontrib>Pralong, François</creatorcontrib><creatorcontrib>Gaillard, Rolf C</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Endocrine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Spinedi, Eduardo</au><au>Voirol, Marie-Jeanne</au><au>Verdumo, Chantal</au><au>Giacominni, Marco</au><au>Pralong, François</au><au>Gaillard, Rolf C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Hypothalamic ghrelin treatment modulates NPY-but not CRH-ergic activity in adrenalectomized rats subjected to food restriction: Evidence of a novel hypothalamic ghrelin effect</atitle><jtitle>Endocrine</jtitle><addtitle>Endocrine</addtitle><date>2006-06-01</date><risdate>2006</risdate><volume>29</volume><issue>3</issue><spage>477</spage><epage>484</epage><pages>477-484</pages><issn>1355-008X</issn><issn>0969-711X</issn><eissn>0969-711X</eissn><abstract>It has been proposed that ghrelin induces food intake by a mechanism due to the stimulation of hypothalamic NPY-ergic activity. It is recognized that bilateral adrenalectomy (ADX) enhances hypothalamic CRH-ergic function and reduces appetite. Thus, the aim of the present study was to test whether, icv-administered, ghrelin modulates NPY- and CRH-ergic functions after food restriction (FR) and glucocorticoid deprivation. For this purpose, 1 microg ghrelin was administered icv to ad libitum (AL) eating and to corticosterone (B)-depleted (ADX) and -replete (sham and ADX+B) male animals habituated, for 15 d, to FR. Food intake, hypothalamic function, and peripheral ghrelin, ACTH, and B concentrations were evaluated 2 h after ghrelin administration. Results indicate that while icv ghrelin treatment stimulated 2-h food intake in AL rats, it failed to do so in sham- and ADX+B-FR animals; moreover, 2-h food intake was inhibited by icv ghrelin treatment in ADX-FR rats. Regarding peripheral hormone levels: (a) basal circulating ghrelin levels, already enhanced (vs AL rats) by FR, significantly increased 2 h after icv ghrelin treatment in AL and sham-FR rats; (b) central ghrelin treatment stimulated ACTH secretion in circulation of AL and glucocorticoid-replete-FR rats; and (c) B circulating levels remained unchanged after ghrelin treatment, although they were in relation to the food intake condition of rats. Finally, hypothalamic NPY mRNA expression was enhanced by FR and, in response to icv ghrelin treatment, it decreased in ADX-FR rats only. ADX-enhanced hypothalamic CRH mRNA levels were reduced by ghrelin icv administration only when animals received B replacement therapy. Our data indicate an inhibitory effect of hypothalamic ghrelin on NPY-ergic activity in FR rats lacking endogenous glucocorticoid.</abstract><cop>United States</cop><pmid>16943587</pmid><doi>10.1385/ENDO:29:3:477</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Endocrine, 2006-06, Vol.29 (3), p.477-484 |
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| subjects | Adrenalectomy Adrenocorticotropic Hormone - blood Animals Body Weight Corticotropin-Releasing Hormone - metabolism Drug Administration Routes Eating - drug effects Food Deprivation - physiology Gene Expression - drug effects Ghrelin Glucocorticoids - physiology Hypothalamus - drug effects Hypothalamus - metabolism Injections - methods Leptin - blood Male Neuropeptide Y - metabolism Peptide Hormones - administration & dosage Peptide Hormones - blood Peptide Hormones - pharmacology Rats Rats, Wistar RNA, Messenger - metabolism |
| title | Hypothalamic ghrelin treatment modulates NPY-but not CRH-ergic activity in adrenalectomized rats subjected to food restriction: Evidence of a novel hypothalamic ghrelin effect |
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