Expression profiles of inflammatory and immune-related genes in Atlantic salmon ( Salmo salar L.) at early time post vaccination
Vaccination of Atlantic salmon parr with oil-based vaccines will inevitably cause inflammation at the site of injection, albeit the underlying mechanisms are not very well understood or studied in any detail. Here, we report time-course changes in expression levels, assessed by real-time RT-PCR of I...
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| Veröffentlicht in: | Vaccine 2005-12, Vol.23 (48), p.5488-5499 |
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| creator | Haugland, Øyvind Torgersen, Jacob Syed, Mohasina Evensen, Øystein |
| description | Vaccination of Atlantic salmon parr with oil-based vaccines will inevitably cause inflammation at the site of injection, albeit the underlying mechanisms are not very well understood or studied in any detail. Here, we report time-course changes in expression levels, assessed by real-time RT-PCR of IL-1β, Mx, two β-2-microglobulin variants and MHC class IIβ, from 2 to 19 days post vaccination with a multi-component oil-adjuvanted vaccine. Highly variable individual responses to vaccination make selection of high responders essential prior to subtractive analysis. Based on the above mentioned expression profiles, high-responding individuals at 2, 8 and 19 days post vaccination, were selected for subtractive analysis. Clustering of clones according to putative function, suggest an initial up-regulation of genes involved in metabolism and cell signalling, before onset of genes involved in inflammation. The lag-time for genes considered as inflammatory markers was more than 48
h, while they were found to constitute the major part of up-regulated transcripts by 8 days post vaccination. By day 19, immune-related genes like immunoglobulin and T cell-receptor genes, comprised a higher proportion of the up-regulated genes than at earlier time points. |
| doi_str_mv | 10.1016/j.vaccine.2005.07.034 |
| format | Article |
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h, while they were found to constitute the major part of up-regulated transcripts by 8 days post vaccination. By day 19, immune-related genes like immunoglobulin and T cell-receptor genes, comprised a higher proportion of the up-regulated genes than at earlier time points.</description><identifier>ISSN: 0264-410X</identifier><identifier>EISSN: 1873-2518</identifier><identifier>DOI: 10.1016/j.vaccine.2005.07.034</identifier><identifier>PMID: 16098640</identifier><identifier>CODEN: VACCDE</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Adjuvants, Immunologic - pharmacology ; Animals ; Applied microbiology ; Atlantic salmon ; Bacterial Vaccines - immunology ; Bacterial Vaccines - pharmacology ; Biological and medical sciences ; Fish ; Fundamental and applied biological sciences. Psychology ; Gene Expression - drug effects ; Gene expression profiles ; Genes ; GTP-Binding Proteins ; Immune system ; Immunity - genetics ; Inflammation - genetics ; Kinases ; Microbiology ; Myxovirus Resistance Proteins ; Salmo salar ; Salmo salar - immunology ; Salmo salar - metabolism ; Salmon ; T cell receptors ; Temperature ; Vaccination ; Vaccination - veterinary ; Vaccines ; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)</subject><ispartof>Vaccine, 2005-12, Vol.23 (48), p.5488-5499</ispartof><rights>2005 Elsevier Ltd</rights><rights>2006 INIST-CNRS</rights><rights>Copyright Elsevier Limited Dec 1, 2005</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c485t-85ae88cfe4e8c89b8be78a8d4afbe6cf69f75d0d1ed32994fb5d82171b1d8a8e3</citedby><cites>FETCH-LOGICAL-c485t-85ae88cfe4e8c89b8be78a8d4afbe6cf69f75d0d1ed32994fb5d82171b1d8a8e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.proquest.com/docview/1558996830?pq-origsite=primo$$EHTML$$P50$$Gproquest$$H</linktohtml><link.rule.ids>314,778,782,3539,27907,27908,45978,64366,64368,64370,72220</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17293391$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16098640$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Haugland, Øyvind</creatorcontrib><creatorcontrib>Torgersen, Jacob</creatorcontrib><creatorcontrib>Syed, Mohasina</creatorcontrib><creatorcontrib>Evensen, Øystein</creatorcontrib><title>Expression profiles of inflammatory and immune-related genes in Atlantic salmon ( Salmo salar L.) at early time post vaccination</title><title>Vaccine</title><addtitle>Vaccine</addtitle><description>Vaccination of Atlantic salmon parr with oil-based vaccines will inevitably cause inflammation at the site of injection, albeit the underlying mechanisms are not very well understood or studied in any detail. Here, we report time-course changes in expression levels, assessed by real-time RT-PCR of IL-1β, Mx, two β-2-microglobulin variants and MHC class IIβ, from 2 to 19 days post vaccination with a multi-component oil-adjuvanted vaccine. Highly variable individual responses to vaccination make selection of high responders essential prior to subtractive analysis. Based on the above mentioned expression profiles, high-responding individuals at 2, 8 and 19 days post vaccination, were selected for subtractive analysis. Clustering of clones according to putative function, suggest an initial up-regulation of genes involved in metabolism and cell signalling, before onset of genes involved in inflammation. The lag-time for genes considered as inflammatory markers was more than 48
h, while they were found to constitute the major part of up-regulated transcripts by 8 days post vaccination. By day 19, immune-related genes like immunoglobulin and T cell-receptor genes, comprised a higher proportion of the up-regulated genes than at earlier time points.</description><subject>Adjuvants, Immunologic - pharmacology</subject><subject>Animals</subject><subject>Applied microbiology</subject><subject>Atlantic salmon</subject><subject>Bacterial Vaccines - immunology</subject><subject>Bacterial Vaccines - pharmacology</subject><subject>Biological and medical sciences</subject><subject>Fish</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression - drug effects</subject><subject>Gene expression profiles</subject><subject>Genes</subject><subject>GTP-Binding Proteins</subject><subject>Immune system</subject><subject>Immunity - genetics</subject><subject>Inflammation - genetics</subject><subject>Kinases</subject><subject>Microbiology</subject><subject>Myxovirus Resistance Proteins</subject><subject>Salmo salar</subject><subject>Salmo salar - immunology</subject><subject>Salmo salar - metabolism</subject><subject>Salmon</subject><subject>T cell receptors</subject><subject>Temperature</subject><subject>Vaccination</subject><subject>Vaccination - veterinary</subject><subject>Vaccines</subject><subject>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)</subject><issn>0264-410X</issn><issn>1873-2518</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqF0U-L1DAYBvAiiju7-hGUgCjroTVpm3-nZVl2VRjwoIK3kCZvJEObjkm6ODc_uikzsODBPTWFX573DU9VvSK4IZiwD7vmXhvjAzQtxrTBvMFd_6TaEMG7uqVEPK02uGV93RP846w6T2mHC-yIfF6dEYalYD3eVH9uf-8jpOTngPZxdn6EhGaHfHCjniad53hAOljkp2kJUEcYdQaLfkIo0Ad0nUcdsjco6XEqIZfo63pYf3VE2-Y90hmBjuMBZT8B2s8po-PqOpepL6pnTo8JXp6-F9X3u9tvN5_q7ZePn2-ut7XpBc21oBqEMA56EEbIQQzAhRa2124AZhyTjlOLLQHbtVL2bqBWtISTgdjioLuo3h1zyyt_LZCymnwyMJbtYV6SYoJLyTh_FBLe9mUELvDy_5BizCmjbM188w_dzUsM5b1FUVEGi24NpEdl4pxSBKf20U86HhTBai1d7dSpdLWWrjBXpfRy7_UpfRkmsA-3Ti0X8PYEdDJ6dFEH49OD463sOkmKuzo6KEXce4gqGQ_BgPURTFZ29o-s8hduFs4V</recordid><startdate>20051201</startdate><enddate>20051201</enddate><creator>Haugland, Øyvind</creator><creator>Torgersen, Jacob</creator><creator>Syed, Mohasina</creator><creator>Evensen, Øystein</creator><general>Elsevier Ltd</general><general>Elsevier</general><general>Elsevier Limited</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7RV</scope><scope>7T2</scope><scope>7T5</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88C</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9-</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0R</scope><scope>M0S</scope><scope>M0T</scope><scope>M1P</scope><scope>M2O</scope><scope>M7N</scope><scope>M7P</scope><scope>MBDVC</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>F1W</scope><scope>H99</scope><scope>L.F</scope><scope>L.G</scope><scope>7X8</scope></search><sort><creationdate>20051201</creationdate><title>Expression profiles of inflammatory and immune-related genes in Atlantic salmon ( Salmo salar L.) at early time post vaccination</title><author>Haugland, Øyvind ; Torgersen, Jacob ; Syed, Mohasina ; Evensen, Øystein</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c485t-85ae88cfe4e8c89b8be78a8d4afbe6cf69f75d0d1ed32994fb5d82171b1d8a8e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Adjuvants, Immunologic - pharmacology</topic><topic>Animals</topic><topic>Applied microbiology</topic><topic>Atlantic salmon</topic><topic>Bacterial Vaccines - immunology</topic><topic>Bacterial Vaccines - pharmacology</topic><topic>Biological and medical sciences</topic><topic>Fish</topic><topic>Fundamental and applied biological sciences. 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Academic</collection><jtitle>Vaccine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Haugland, Øyvind</au><au>Torgersen, Jacob</au><au>Syed, Mohasina</au><au>Evensen, Øystein</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression profiles of inflammatory and immune-related genes in Atlantic salmon ( Salmo salar L.) at early time post vaccination</atitle><jtitle>Vaccine</jtitle><addtitle>Vaccine</addtitle><date>2005-12-01</date><risdate>2005</risdate><volume>23</volume><issue>48</issue><spage>5488</spage><epage>5499</epage><pages>5488-5499</pages><issn>0264-410X</issn><eissn>1873-2518</eissn><coden>VACCDE</coden><abstract>Vaccination of Atlantic salmon parr with oil-based vaccines will inevitably cause inflammation at the site of injection, albeit the underlying mechanisms are not very well understood or studied in any detail. Here, we report time-course changes in expression levels, assessed by real-time RT-PCR of IL-1β, Mx, two β-2-microglobulin variants and MHC class IIβ, from 2 to 19 days post vaccination with a multi-component oil-adjuvanted vaccine. Highly variable individual responses to vaccination make selection of high responders essential prior to subtractive analysis. Based on the above mentioned expression profiles, high-responding individuals at 2, 8 and 19 days post vaccination, were selected for subtractive analysis. Clustering of clones according to putative function, suggest an initial up-regulation of genes involved in metabolism and cell signalling, before onset of genes involved in inflammation. The lag-time for genes considered as inflammatory markers was more than 48
h, while they were found to constitute the major part of up-regulated transcripts by 8 days post vaccination. By day 19, immune-related genes like immunoglobulin and T cell-receptor genes, comprised a higher proportion of the up-regulated genes than at earlier time points.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>16098640</pmid><doi>10.1016/j.vaccine.2005.07.034</doi><tpages>12</tpages></addata></record> |
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| subjects | Adjuvants, Immunologic - pharmacology Animals Applied microbiology Atlantic salmon Bacterial Vaccines - immunology Bacterial Vaccines - pharmacology Biological and medical sciences Fish Fundamental and applied biological sciences. Psychology Gene Expression - drug effects Gene expression profiles Genes GTP-Binding Proteins Immune system Immunity - genetics Inflammation - genetics Kinases Microbiology Myxovirus Resistance Proteins Salmo salar Salmo salar - immunology Salmo salar - metabolism Salmon T cell receptors Temperature Vaccination Vaccination - veterinary Vaccines Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects) |
| title | Expression profiles of inflammatory and immune-related genes in Atlantic salmon ( Salmo salar L.) at early time post vaccination |
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