Dextran−Gold Nanoparticle Hybrid Material for Biomolecule Immobilization and Detection
The formation of a hybrid metal−biopolymer material is described. The synthesis of this material consists of functionalizing the surface of gold nanoparticles through a series of steps that lead to epoxy-functionalized nanoparticles. These are subsequently reacted with hydroxyl moieties of the α-d-g...
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| Veröffentlicht in: | Analytical chemistry (Washington) 2005-11, Vol.77 (22), p.7204-7211 |
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| creator | Lee, Sunmook Pérez-Luna, Víctor H |
| description | The formation of a hybrid metal−biopolymer material is described. The synthesis of this material consists of functionalizing the surface of gold nanoparticles through a series of steps that lead to epoxy-functionalized nanoparticles. These are subsequently reacted with hydroxyl moieties of the α-d-glucopyranosyl groups of dextran. Subsequently, the dextran chains are carboxylated through treatment with bromoacetic acid. The resultant material combines the unique optical properties of gold nanoparticles with the versatility that carboxylated dextran offers for further functionalization with biomolecules. The interaction of this material with three proteins was then investigated through changes in the plasmon resonance properties of the gold nanoparticles. Concanavalin A, a lectin that binds glucose and mannose by means of specific molecular recognition, interacts readily with this material and such interaction is easily detected using optical absorption spectroscopy. Through reaction of the carboxyl groups with (+)-biotinyl-3,6,9,-trioxaundecanediamine, a material bearing biotin groups was obtained. This could interact with streptavidin or antibiotin by means of specific molecular recognition. Further confirmation of biospecific interactions was obtained with control experiments in which the binding sites were blocked through preincubation of the proteins with the corresponding ligand in solution. Binding of these proteins was concentration-dependent over a wide concentration range. This material provides a simple and convenient colorimetric method for biospecific interaction analysis. |
| doi_str_mv | 10.1021/ac050484n |
| format | Article |
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The synthesis of this material consists of functionalizing the surface of gold nanoparticles through a series of steps that lead to epoxy-functionalized nanoparticles. These are subsequently reacted with hydroxyl moieties of the α-d-glucopyranosyl groups of dextran. Subsequently, the dextran chains are carboxylated through treatment with bromoacetic acid. The resultant material combines the unique optical properties of gold nanoparticles with the versatility that carboxylated dextran offers for further functionalization with biomolecules. The interaction of this material with three proteins was then investigated through changes in the plasmon resonance properties of the gold nanoparticles. Concanavalin A, a lectin that binds glucose and mannose by means of specific molecular recognition, interacts readily with this material and such interaction is easily detected using optical absorption spectroscopy. Through reaction of the carboxyl groups with (+)-biotinyl-3,6,9,-trioxaundecanediamine, a material bearing biotin groups was obtained. This could interact with streptavidin or antibiotin by means of specific molecular recognition. Further confirmation of biospecific interactions was obtained with control experiments in which the binding sites were blocked through preincubation of the proteins with the corresponding ligand in solution. Binding of these proteins was concentration-dependent over a wide concentration range. This material provides a simple and convenient colorimetric method for biospecific interaction analysis.</description><identifier>ISSN: 0003-2700</identifier><identifier>EISSN: 1520-6882</identifier><identifier>DOI: 10.1021/ac050484n</identifier><identifier>PMID: 16285667</identifier><identifier>CODEN: ANCHAM</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>Analytical chemistry ; Biotin - chemistry ; Biotin - metabolism ; Chemical bonds ; Chemistry ; Colloids - chemistry ; Dextrans - chemistry ; Exact sciences and technology ; Gold - chemistry ; Microscopy, Electron, Transmission ; Molecular Structure ; Nanoparticles ; Nanoparticles - chemistry ; Proteins ; Spectrometric and optical methods ; Spectrum Analysis ; Streptavidin - metabolism</subject><ispartof>Analytical chemistry (Washington), 2005-11, Vol.77 (22), p.7204-7211</ispartof><rights>Copyright © 2005 American Chemical Society</rights><rights>2006 INIST-CNRS</rights><rights>Copyright American Chemical Society Nov 15, 2005</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a505t-a410e762c7d744144841fcf13e4d4e5146da89d019a88ce9bd9080fb7a3914f03</citedby><cites>FETCH-LOGICAL-a505t-a410e762c7d744144841fcf13e4d4e5146da89d019a88ce9bd9080fb7a3914f03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/ac050484n$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/ac050484n$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,776,780,2752,27053,27901,27902,56713,56763</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17293879$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16285667$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lee, Sunmook</creatorcontrib><creatorcontrib>Pérez-Luna, Víctor H</creatorcontrib><title>Dextran−Gold Nanoparticle Hybrid Material for Biomolecule Immobilization and Detection</title><title>Analytical chemistry (Washington)</title><addtitle>Anal. Chem</addtitle><description>The formation of a hybrid metal−biopolymer material is described. The synthesis of this material consists of functionalizing the surface of gold nanoparticles through a series of steps that lead to epoxy-functionalized nanoparticles. These are subsequently reacted with hydroxyl moieties of the α-d-glucopyranosyl groups of dextran. Subsequently, the dextran chains are carboxylated through treatment with bromoacetic acid. The resultant material combines the unique optical properties of gold nanoparticles with the versatility that carboxylated dextran offers for further functionalization with biomolecules. The interaction of this material with three proteins was then investigated through changes in the plasmon resonance properties of the gold nanoparticles. Concanavalin A, a lectin that binds glucose and mannose by means of specific molecular recognition, interacts readily with this material and such interaction is easily detected using optical absorption spectroscopy. Through reaction of the carboxyl groups with (+)-biotinyl-3,6,9,-trioxaundecanediamine, a material bearing biotin groups was obtained. This could interact with streptavidin or antibiotin by means of specific molecular recognition. Further confirmation of biospecific interactions was obtained with control experiments in which the binding sites were blocked through preincubation of the proteins with the corresponding ligand in solution. Binding of these proteins was concentration-dependent over a wide concentration range. This material provides a simple and convenient colorimetric method for biospecific interaction analysis.</description><subject>Analytical chemistry</subject><subject>Biotin - chemistry</subject><subject>Biotin - metabolism</subject><subject>Chemical bonds</subject><subject>Chemistry</subject><subject>Colloids - chemistry</subject><subject>Dextrans - chemistry</subject><subject>Exact sciences and technology</subject><subject>Gold - chemistry</subject><subject>Microscopy, Electron, Transmission</subject><subject>Molecular Structure</subject><subject>Nanoparticles</subject><subject>Nanoparticles - chemistry</subject><subject>Proteins</subject><subject>Spectrometric and optical methods</subject><subject>Spectrum Analysis</subject><subject>Streptavidin - metabolism</subject><issn>0003-2700</issn><issn>1520-6882</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0c9u1DAQBnALgei2cOAFUIQEEofATOLYzrG0pa1UYCWWPzdr4jiSSxIvdiK1PAFnHpEnwatddSU4cLIs__Rpxh9jTxBeIRT4mgxUwBUf77EFVgXkQqniPlsAQJkXEuCAHcZ4DYAIKB6yAxSFqoSQC_b11N5MgcbfP3-d-77N3tPo1xQmZ3qbXdw2wbXZO5pscNRnnQ_ZG-cH31szp_fLYfCN690PmpwfMxrb7NRO1mxuj9iDjvpoH-_OI_bp7dnq5CK_-nB-eXJ8lVMF1ZQTR7BSFEa2knPkaQvsTIel5S23FXLRkqpbwJqUMrZu2hoUdI2kskbeQXnEXmxz18F_n22c9OCisX1Po_Vz1ELJuoaS_xdiXSqZ_ifBZ3_Baz-HMS2hC5RKCRQyoZdbZIKPMdhOr4MbKNxqBL0pRd-VkuzTXeDcDLbdy10LCTzfAYqG-i71YVzcO1lsZquTy7fOxcne3L1T-KZTiqz0avlRr5Rcfvm8WurlPpdM3C_x74B_APDoryA</recordid><startdate>20051115</startdate><enddate>20051115</enddate><creator>Lee, Sunmook</creator><creator>Pérez-Luna, Víctor H</creator><general>American Chemical Society</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7TM</scope><scope>7U5</scope><scope>7U7</scope><scope>7U9</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20051115</creationdate><title>Dextran−Gold Nanoparticle Hybrid Material for Biomolecule Immobilization and Detection</title><author>Lee, Sunmook ; Pérez-Luna, Víctor H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a505t-a410e762c7d744144841fcf13e4d4e5146da89d019a88ce9bd9080fb7a3914f03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Analytical chemistry</topic><topic>Biotin - chemistry</topic><topic>Biotin - metabolism</topic><topic>Chemical bonds</topic><topic>Chemistry</topic><topic>Colloids - chemistry</topic><topic>Dextrans - chemistry</topic><topic>Exact sciences and technology</topic><topic>Gold - chemistry</topic><topic>Microscopy, Electron, Transmission</topic><topic>Molecular Structure</topic><topic>Nanoparticles</topic><topic>Nanoparticles - chemistry</topic><topic>Proteins</topic><topic>Spectrometric and optical methods</topic><topic>Spectrum Analysis</topic><topic>Streptavidin - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lee, Sunmook</creatorcontrib><creatorcontrib>Pérez-Luna, Víctor H</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical chemistry (Washington)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lee, Sunmook</au><au>Pérez-Luna, Víctor H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Dextran−Gold Nanoparticle Hybrid Material for Biomolecule Immobilization and Detection</atitle><jtitle>Analytical chemistry (Washington)</jtitle><addtitle>Anal. Chem</addtitle><date>2005-11-15</date><risdate>2005</risdate><volume>77</volume><issue>22</issue><spage>7204</spage><epage>7211</epage><pages>7204-7211</pages><issn>0003-2700</issn><eissn>1520-6882</eissn><coden>ANCHAM</coden><abstract>The formation of a hybrid metal−biopolymer material is described. The synthesis of this material consists of functionalizing the surface of gold nanoparticles through a series of steps that lead to epoxy-functionalized nanoparticles. These are subsequently reacted with hydroxyl moieties of the α-d-glucopyranosyl groups of dextran. Subsequently, the dextran chains are carboxylated through treatment with bromoacetic acid. The resultant material combines the unique optical properties of gold nanoparticles with the versatility that carboxylated dextran offers for further functionalization with biomolecules. The interaction of this material with three proteins was then investigated through changes in the plasmon resonance properties of the gold nanoparticles. Concanavalin A, a lectin that binds glucose and mannose by means of specific molecular recognition, interacts readily with this material and such interaction is easily detected using optical absorption spectroscopy. Through reaction of the carboxyl groups with (+)-biotinyl-3,6,9,-trioxaundecanediamine, a material bearing biotin groups was obtained. This could interact with streptavidin or antibiotin by means of specific molecular recognition. Further confirmation of biospecific interactions was obtained with control experiments in which the binding sites were blocked through preincubation of the proteins with the corresponding ligand in solution. Binding of these proteins was concentration-dependent over a wide concentration range. This material provides a simple and convenient colorimetric method for biospecific interaction analysis.</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>16285667</pmid><doi>10.1021/ac050484n</doi><tpages>8</tpages></addata></record> |
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| ispartof | Analytical chemistry (Washington), 2005-11, Vol.77 (22), p.7204-7211 |
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| source | ACS Publications; MEDLINE |
| subjects | Analytical chemistry Biotin - chemistry Biotin - metabolism Chemical bonds Chemistry Colloids - chemistry Dextrans - chemistry Exact sciences and technology Gold - chemistry Microscopy, Electron, Transmission Molecular Structure Nanoparticles Nanoparticles - chemistry Proteins Spectrometric and optical methods Spectrum Analysis Streptavidin - metabolism |
| title | Dextran−Gold Nanoparticle Hybrid Material for Biomolecule Immobilization and Detection |
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