Analysis of Lipoproteins by Microchip Electrophoresis with High Speed and High Reproducibility
A method for the fast analysis of lipoproteins by microchip electrophoresis with light-emitting diode confocal fluorescence detection has been developed. Lipoproteins labeled with BODIPY FL C5-ceramide are found to strongly adsorb on the bare surface of a poly(methyl methacrylate) (PMMA) microchip....
Gespeichert in:
| Veröffentlicht in: | Analytical chemistry (Washington) 2005-11, Vol.77 (22), p.7282-7287 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 7287 |
|---|---|
| container_issue | 22 |
| container_start_page | 7282 |
| container_title | Analytical chemistry (Washington) |
| container_volume | 77 |
| creator | Ping, Guichen Zhu, Bingmei Jabasini, Mohammad Xu, Feng Oka, Hiroaki Sugihara, Hirokazu Baba, Yoshinobu |
| description | A method for the fast analysis of lipoproteins by microchip electrophoresis with light-emitting diode confocal fluorescence detection has been developed. Lipoproteins labeled with BODIPY FL C5-ceramide are found to strongly adsorb on the bare surface of a poly(methyl methacrylate) (PMMA) microchip. Sodium dodecyl sulfate and cetyltrimethylammonium bromide were therefore utilized to alter lipoproteins and channel surface to make them bear the same type of charge. After modification, the peak shape of lipoproteins was greatly improved, demonstrating lipoprotein adsorption on a PMMA chip dramatically reduced due to electrostatic repulsion. In addition, polymers were added into the running buffer to suppress electroosmotic flow and to serve as a sieving matrix. As a result, lipoprotein separation was manipulated by both electrophoretic mobilities and particle sizes. Various separation parameters including surfactant concentration, buffer pH, and polymer concentration as well as on-line concentration were investigated systematically. Under optimal conditions, two baseline separations of standard lipoproteins including high-density lipoprotein, low-density lipoprotein, and very low-density lipoprotein were achieved with different selectivity. This method affords high separation speed (within 100 s) and high reproducibility. The intraassay and interassay RSDs of lipoprotein migration times were in the range of 0.90−1.9%, indicating this method is highly reliable. |
| doi_str_mv | 10.1021/ac050896w |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_68797709</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>940618941</sourcerecordid><originalsourceid>FETCH-LOGICAL-a439t-19bbe28707c4181169697efa01aff3a8580b84982292743a0bb7e07897a195453</originalsourceid><addsrcrecordid>eNqF0VFrFDEQB_Agij2rD34BWQQFH1Yn2U0meaylWuHEYuurYTaX9VL3dtdkl3rf3hx39EAffAohP_6TmWHsOYe3HAR_Rw4kaKPuHrAFlwJKpbV4yBYAUJUCAU7Yk5RuATgHrh6zE66ElgrVgn0_66nbppCKoS2WYRzGOEw-9KlotsXn4OLg1mEsLjrvpjiM6yH6Hb4L07q4DD_WxfXo_aqgfrW_fvU5YDW70IQuTNun7FFLXfLPDucp-_bh4ub8slx--fjp_GxZUl2ZqeSmabzQCOhqrjlXRhn0LQGntq1ISw2Nro0WwgisK4KmQQ-oDRI3spbVKXu9z83Vf80-TXYTkvNdR70f5mSVRoMI5r-Qm8oACMzw5V_wdphjHlaygqPJo6x1Rm_2KM8ppehbO8awobi1HOxuNfZ-Ndm-OATOzcavjvKwiwxeHQAlR10bqXchHR0KU2m9K1ruXUiT_33_TvGnVVihtDdX1xZ1bvbqvbTymEsuHZv494N_AMEUrwA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>217901148</pqid></control><display><type>article</type><title>Analysis of Lipoproteins by Microchip Electrophoresis with High Speed and High Reproducibility</title><source>MEDLINE</source><source>ACS Publications</source><creator>Ping, Guichen ; Zhu, Bingmei ; Jabasini, Mohammad ; Xu, Feng ; Oka, Hiroaki ; Sugihara, Hirokazu ; Baba, Yoshinobu</creator><creatorcontrib>Ping, Guichen ; Zhu, Bingmei ; Jabasini, Mohammad ; Xu, Feng ; Oka, Hiroaki ; Sugihara, Hirokazu ; Baba, Yoshinobu</creatorcontrib><description>A method for the fast analysis of lipoproteins by microchip electrophoresis with light-emitting diode confocal fluorescence detection has been developed. Lipoproteins labeled with BODIPY FL C5-ceramide are found to strongly adsorb on the bare surface of a poly(methyl methacrylate) (PMMA) microchip. Sodium dodecyl sulfate and cetyltrimethylammonium bromide were therefore utilized to alter lipoproteins and channel surface to make them bear the same type of charge. After modification, the peak shape of lipoproteins was greatly improved, demonstrating lipoprotein adsorption on a PMMA chip dramatically reduced due to electrostatic repulsion. In addition, polymers were added into the running buffer to suppress electroosmotic flow and to serve as a sieving matrix. As a result, lipoprotein separation was manipulated by both electrophoretic mobilities and particle sizes. Various separation parameters including surfactant concentration, buffer pH, and polymer concentration as well as on-line concentration were investigated systematically. Under optimal conditions, two baseline separations of standard lipoproteins including high-density lipoprotein, low-density lipoprotein, and very low-density lipoprotein were achieved with different selectivity. This method affords high separation speed (within 100 s) and high reproducibility. The intraassay and interassay RSDs of lipoprotein migration times were in the range of 0.90−1.9%, indicating this method is highly reliable.</description><identifier>ISSN: 0003-2700</identifier><identifier>EISSN: 1520-6882</identifier><identifier>DOI: 10.1021/ac050896w</identifier><identifier>PMID: 16285676</identifier><identifier>CODEN: ANCHAM</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>Analytical chemistry ; Chemistry ; Chromatographic methods and physical methods associated with chromatography ; Diodes ; Electrophoresis, Microchip - methods ; Exact sciences and technology ; Fluorescence ; Hydrogen-Ion Concentration ; Lipoproteins - analysis ; Online Systems ; Other chromatographic methods ; Polymers - chemistry ; Proteins ; Reproducibility of Results ; Sodium Dodecyl Sulfate ; Time Factors</subject><ispartof>Analytical chemistry (Washington), 2005-11, Vol.77 (22), p.7282-7287</ispartof><rights>Copyright © 2005 American Chemical Society</rights><rights>2006 INIST-CNRS</rights><rights>Copyright American Chemical Society Nov 15, 2005</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a439t-19bbe28707c4181169697efa01aff3a8580b84982292743a0bb7e07897a195453</citedby><cites>FETCH-LOGICAL-a439t-19bbe28707c4181169697efa01aff3a8580b84982292743a0bb7e07897a195453</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/ac050896w$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/ac050896w$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>315,781,785,2766,27081,27929,27930,56743,56793</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17293888$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16285676$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ping, Guichen</creatorcontrib><creatorcontrib>Zhu, Bingmei</creatorcontrib><creatorcontrib>Jabasini, Mohammad</creatorcontrib><creatorcontrib>Xu, Feng</creatorcontrib><creatorcontrib>Oka, Hiroaki</creatorcontrib><creatorcontrib>Sugihara, Hirokazu</creatorcontrib><creatorcontrib>Baba, Yoshinobu</creatorcontrib><title>Analysis of Lipoproteins by Microchip Electrophoresis with High Speed and High Reproducibility</title><title>Analytical chemistry (Washington)</title><addtitle>Anal. Chem</addtitle><description>A method for the fast analysis of lipoproteins by microchip electrophoresis with light-emitting diode confocal fluorescence detection has been developed. Lipoproteins labeled with BODIPY FL C5-ceramide are found to strongly adsorb on the bare surface of a poly(methyl methacrylate) (PMMA) microchip. Sodium dodecyl sulfate and cetyltrimethylammonium bromide were therefore utilized to alter lipoproteins and channel surface to make them bear the same type of charge. After modification, the peak shape of lipoproteins was greatly improved, demonstrating lipoprotein adsorption on a PMMA chip dramatically reduced due to electrostatic repulsion. In addition, polymers were added into the running buffer to suppress electroosmotic flow and to serve as a sieving matrix. As a result, lipoprotein separation was manipulated by both electrophoretic mobilities and particle sizes. Various separation parameters including surfactant concentration, buffer pH, and polymer concentration as well as on-line concentration were investigated systematically. Under optimal conditions, two baseline separations of standard lipoproteins including high-density lipoprotein, low-density lipoprotein, and very low-density lipoprotein were achieved with different selectivity. This method affords high separation speed (within 100 s) and high reproducibility. The intraassay and interassay RSDs of lipoprotein migration times were in the range of 0.90−1.9%, indicating this method is highly reliable.</description><subject>Analytical chemistry</subject><subject>Chemistry</subject><subject>Chromatographic methods and physical methods associated with chromatography</subject><subject>Diodes</subject><subject>Electrophoresis, Microchip - methods</subject><subject>Exact sciences and technology</subject><subject>Fluorescence</subject><subject>Hydrogen-Ion Concentration</subject><subject>Lipoproteins - analysis</subject><subject>Online Systems</subject><subject>Other chromatographic methods</subject><subject>Polymers - chemistry</subject><subject>Proteins</subject><subject>Reproducibility of Results</subject><subject>Sodium Dodecyl Sulfate</subject><subject>Time Factors</subject><issn>0003-2700</issn><issn>1520-6882</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0VFrFDEQB_Agij2rD34BWQQFH1Yn2U0meaylWuHEYuurYTaX9VL3dtdkl3rf3hx39EAffAohP_6TmWHsOYe3HAR_Rw4kaKPuHrAFlwJKpbV4yBYAUJUCAU7Yk5RuATgHrh6zE66ElgrVgn0_66nbppCKoS2WYRzGOEw-9KlotsXn4OLg1mEsLjrvpjiM6yH6Hb4L07q4DD_WxfXo_aqgfrW_fvU5YDW70IQuTNun7FFLXfLPDucp-_bh4ub8slx--fjp_GxZUl2ZqeSmabzQCOhqrjlXRhn0LQGntq1ISw2Nro0WwgisK4KmQQ-oDRI3spbVKXu9z83Vf80-TXYTkvNdR70f5mSVRoMI5r-Qm8oACMzw5V_wdphjHlaygqPJo6x1Rm_2KM8ppehbO8awobi1HOxuNfZ-Ndm-OATOzcavjvKwiwxeHQAlR10bqXchHR0KU2m9K1ruXUiT_33_TvGnVVihtDdX1xZ1bvbqvbTymEsuHZv494N_AMEUrwA</recordid><startdate>20051115</startdate><enddate>20051115</enddate><creator>Ping, Guichen</creator><creator>Zhu, Bingmei</creator><creator>Jabasini, Mohammad</creator><creator>Xu, Feng</creator><creator>Oka, Hiroaki</creator><creator>Sugihara, Hirokazu</creator><creator>Baba, Yoshinobu</creator><general>American Chemical Society</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7TM</scope><scope>7U5</scope><scope>7U7</scope><scope>7U9</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20051115</creationdate><title>Analysis of Lipoproteins by Microchip Electrophoresis with High Speed and High Reproducibility</title><author>Ping, Guichen ; Zhu, Bingmei ; Jabasini, Mohammad ; Xu, Feng ; Oka, Hiroaki ; Sugihara, Hirokazu ; Baba, Yoshinobu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a439t-19bbe28707c4181169697efa01aff3a8580b84982292743a0bb7e07897a195453</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Analytical chemistry</topic><topic>Chemistry</topic><topic>Chromatographic methods and physical methods associated with chromatography</topic><topic>Diodes</topic><topic>Electrophoresis, Microchip - methods</topic><topic>Exact sciences and technology</topic><topic>Fluorescence</topic><topic>Hydrogen-Ion Concentration</topic><topic>Lipoproteins - analysis</topic><topic>Online Systems</topic><topic>Other chromatographic methods</topic><topic>Polymers - chemistry</topic><topic>Proteins</topic><topic>Reproducibility of Results</topic><topic>Sodium Dodecyl Sulfate</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ping, Guichen</creatorcontrib><creatorcontrib>Zhu, Bingmei</creatorcontrib><creatorcontrib>Jabasini, Mohammad</creatorcontrib><creatorcontrib>Xu, Feng</creatorcontrib><creatorcontrib>Oka, Hiroaki</creatorcontrib><creatorcontrib>Sugihara, Hirokazu</creatorcontrib><creatorcontrib>Baba, Yoshinobu</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical chemistry (Washington)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ping, Guichen</au><au>Zhu, Bingmei</au><au>Jabasini, Mohammad</au><au>Xu, Feng</au><au>Oka, Hiroaki</au><au>Sugihara, Hirokazu</au><au>Baba, Yoshinobu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Analysis of Lipoproteins by Microchip Electrophoresis with High Speed and High Reproducibility</atitle><jtitle>Analytical chemistry (Washington)</jtitle><addtitle>Anal. Chem</addtitle><date>2005-11-15</date><risdate>2005</risdate><volume>77</volume><issue>22</issue><spage>7282</spage><epage>7287</epage><pages>7282-7287</pages><issn>0003-2700</issn><eissn>1520-6882</eissn><coden>ANCHAM</coden><abstract>A method for the fast analysis of lipoproteins by microchip electrophoresis with light-emitting diode confocal fluorescence detection has been developed. Lipoproteins labeled with BODIPY FL C5-ceramide are found to strongly adsorb on the bare surface of a poly(methyl methacrylate) (PMMA) microchip. Sodium dodecyl sulfate and cetyltrimethylammonium bromide were therefore utilized to alter lipoproteins and channel surface to make them bear the same type of charge. After modification, the peak shape of lipoproteins was greatly improved, demonstrating lipoprotein adsorption on a PMMA chip dramatically reduced due to electrostatic repulsion. In addition, polymers were added into the running buffer to suppress electroosmotic flow and to serve as a sieving matrix. As a result, lipoprotein separation was manipulated by both electrophoretic mobilities and particle sizes. Various separation parameters including surfactant concentration, buffer pH, and polymer concentration as well as on-line concentration were investigated systematically. Under optimal conditions, two baseline separations of standard lipoproteins including high-density lipoprotein, low-density lipoprotein, and very low-density lipoprotein were achieved with different selectivity. This method affords high separation speed (within 100 s) and high reproducibility. The intraassay and interassay RSDs of lipoprotein migration times were in the range of 0.90−1.9%, indicating this method is highly reliable.</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>16285676</pmid><doi>10.1021/ac050896w</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0003-2700 |
| ispartof | Analytical chemistry (Washington), 2005-11, Vol.77 (22), p.7282-7287 |
| issn | 0003-2700 1520-6882 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_68797709 |
| source | MEDLINE; ACS Publications |
| subjects | Analytical chemistry Chemistry Chromatographic methods and physical methods associated with chromatography Diodes Electrophoresis, Microchip - methods Exact sciences and technology Fluorescence Hydrogen-Ion Concentration Lipoproteins - analysis Online Systems Other chromatographic methods Polymers - chemistry Proteins Reproducibility of Results Sodium Dodecyl Sulfate Time Factors |
| title | Analysis of Lipoproteins by Microchip Electrophoresis with High Speed and High Reproducibility |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-14T05%3A46%3A46IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Analysis%20of%20Lipoproteins%20by%20Microchip%20Electrophoresis%20with%20High%20Speed%20and%20High%20Reproducibility&rft.jtitle=Analytical%20chemistry%20(Washington)&rft.au=Ping,%20Guichen&rft.date=2005-11-15&rft.volume=77&rft.issue=22&rft.spage=7282&rft.epage=7287&rft.pages=7282-7287&rft.issn=0003-2700&rft.eissn=1520-6882&rft.coden=ANCHAM&rft_id=info:doi/10.1021/ac050896w&rft_dat=%3Cproquest_cross%3E940618941%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=217901148&rft_id=info:pmid/16285676&rfr_iscdi=true |