Construction, database integration, and application of an Oenothera EST library
Coevolution of cellular genetic compartments is a fundamental aspect in eukaryotic genome evolution that becomes apparent in serious developmental disturbances after interspecific organelle exchanges. The genus Oenothera represents a unique, at present the only available, resource to study the role...
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| Veröffentlicht in: | Genomics (San Diego, Calif.) Calif.), 2006-09, Vol.88 (3), p.372-380 |
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| creator | Mráček, Jaroslav Greiner, Stephan Cho, Won Kyong Rauwolf, Uwe Braun, Martha Umate, Pavan Altstätter, Johannes Stoppel, Rhea Mlčochová, Lada Silber, Martina V. Volz, Stefanie M. White, Sarah Selmeier, Renate Rudd, Stephen Herrmann, Reinhold G. Meurer, Jörg |
| description | Coevolution of cellular genetic compartments is a fundamental aspect in eukaryotic genome evolution that becomes apparent in serious developmental disturbances after interspecific organelle exchanges. The genus
Oenothera represents a unique, at present the only available, resource to study the role of the compartmentalized plant genome in diversification of populations and speciation processes. An integrated approach involving cDNA cloning, EST sequencing, and bioinformatic data mining was chosen using
Oenothera elata with the genetic constitution nuclear genome AA with plastome type I. The Gene Ontology system grouped 1621 unique gene products into 17 different functional categories. Application of arrays generated from a selected fraction of ESTs revealed significantly differing expression profiles among closely related
Oenothera species possessing the potential to generate fertile and incompatible plastid/nuclear hybrids (hybrid bleaching). Furthermore, the EST library provides a valuable source of PCR-based polymorphic molecular markers that are instrumental for genotyping and molecular mapping approaches. |
| doi_str_mv | 10.1016/j.ygeno.2006.05.010 |
| format | Article |
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Oenothera represents a unique, at present the only available, resource to study the role of the compartmentalized plant genome in diversification of populations and speciation processes. An integrated approach involving cDNA cloning, EST sequencing, and bioinformatic data mining was chosen using
Oenothera elata with the genetic constitution nuclear genome AA with plastome type I. The Gene Ontology system grouped 1621 unique gene products into 17 different functional categories. Application of arrays generated from a selected fraction of ESTs revealed significantly differing expression profiles among closely related
Oenothera species possessing the potential to generate fertile and incompatible plastid/nuclear hybrids (hybrid bleaching). Furthermore, the EST library provides a valuable source of PCR-based polymorphic molecular markers that are instrumental for genotyping and molecular mapping approaches.</description><identifier>ISSN: 0888-7543</identifier><identifier>EISSN: 1089-8646</identifier><identifier>DOI: 10.1016/j.ygeno.2006.05.010</identifier><identifier>PMID: 16829020</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>bioinformatics ; Biological and medical sciences ; Cell Nucleus - genetics ; Chromosome Mapping - methods ; Codominant molecular marker ; complementary DNA ; DNA libraries ; EST database ; Eukaryotic genome evolution ; Expressed Sequence Tags ; Expression profiling ; Fundamental and applied biological sciences. Psychology ; gene banks ; Gene Library ; genes ; Genes. Genome ; Genetic Markers - genetics ; Genetics of eukaryotes. Biological and molecular evolution ; Genome/plastome incompatibility ; Interspecific genome/plastome hybrids and cybrids ; Molecular and cellular biology ; molecular cloning ; Molecular genetics ; molecular sequence data ; nucleotide sequences ; Oenothera ; Oenothera - genetics ; Oenothera elata ; Plant Infertility - genetics ; Plastids - genetics</subject><ispartof>Genomics (San Diego, Calif.), 2006-09, Vol.88 (3), p.372-380</ispartof><rights>2006 Elsevier Inc.</rights><rights>2006 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c553t-7a7de4190bb6585bbd81656c5118eada181afc87231713f63ae5efb1d5d97ba3</citedby><cites>FETCH-LOGICAL-c553t-7a7de4190bb6585bbd81656c5118eada181afc87231713f63ae5efb1d5d97ba3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0888754306001686$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18099502$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16829020$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mráček, Jaroslav</creatorcontrib><creatorcontrib>Greiner, Stephan</creatorcontrib><creatorcontrib>Cho, Won Kyong</creatorcontrib><creatorcontrib>Rauwolf, Uwe</creatorcontrib><creatorcontrib>Braun, Martha</creatorcontrib><creatorcontrib>Umate, Pavan</creatorcontrib><creatorcontrib>Altstätter, Johannes</creatorcontrib><creatorcontrib>Stoppel, Rhea</creatorcontrib><creatorcontrib>Mlčochová, Lada</creatorcontrib><creatorcontrib>Silber, Martina V.</creatorcontrib><creatorcontrib>Volz, Stefanie M.</creatorcontrib><creatorcontrib>White, Sarah</creatorcontrib><creatorcontrib>Selmeier, Renate</creatorcontrib><creatorcontrib>Rudd, Stephen</creatorcontrib><creatorcontrib>Herrmann, Reinhold G.</creatorcontrib><creatorcontrib>Meurer, Jörg</creatorcontrib><title>Construction, database integration, and application of an Oenothera EST library</title><title>Genomics (San Diego, Calif.)</title><addtitle>Genomics</addtitle><description>Coevolution of cellular genetic compartments is a fundamental aspect in eukaryotic genome evolution that becomes apparent in serious developmental disturbances after interspecific organelle exchanges. The genus
Oenothera represents a unique, at present the only available, resource to study the role of the compartmentalized plant genome in diversification of populations and speciation processes. An integrated approach involving cDNA cloning, EST sequencing, and bioinformatic data mining was chosen using
Oenothera elata with the genetic constitution nuclear genome AA with plastome type I. The Gene Ontology system grouped 1621 unique gene products into 17 different functional categories. Application of arrays generated from a selected fraction of ESTs revealed significantly differing expression profiles among closely related
Oenothera species possessing the potential to generate fertile and incompatible plastid/nuclear hybrids (hybrid bleaching). Furthermore, the EST library provides a valuable source of PCR-based polymorphic molecular markers that are instrumental for genotyping and molecular mapping approaches.</description><subject>bioinformatics</subject><subject>Biological and medical sciences</subject><subject>Cell Nucleus - genetics</subject><subject>Chromosome Mapping - methods</subject><subject>Codominant molecular marker</subject><subject>complementary DNA</subject><subject>DNA libraries</subject><subject>EST database</subject><subject>Eukaryotic genome evolution</subject><subject>Expressed Sequence Tags</subject><subject>Expression profiling</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>gene banks</subject><subject>Gene Library</subject><subject>genes</subject><subject>Genes. Genome</subject><subject>Genetic Markers - genetics</subject><subject>Genetics of eukaryotes. 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The genus
Oenothera represents a unique, at present the only available, resource to study the role of the compartmentalized plant genome in diversification of populations and speciation processes. An integrated approach involving cDNA cloning, EST sequencing, and bioinformatic data mining was chosen using
Oenothera elata with the genetic constitution nuclear genome AA with plastome type I. The Gene Ontology system grouped 1621 unique gene products into 17 different functional categories. Application of arrays generated from a selected fraction of ESTs revealed significantly differing expression profiles among closely related
Oenothera species possessing the potential to generate fertile and incompatible plastid/nuclear hybrids (hybrid bleaching). Furthermore, the EST library provides a valuable source of PCR-based polymorphic molecular markers that are instrumental for genotyping and molecular mapping approaches.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>16829020</pmid><doi>10.1016/j.ygeno.2006.05.010</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Elsevier ScienceDirect Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | bioinformatics Biological and medical sciences Cell Nucleus - genetics Chromosome Mapping - methods Codominant molecular marker complementary DNA DNA libraries EST database Eukaryotic genome evolution Expressed Sequence Tags Expression profiling Fundamental and applied biological sciences. Psychology gene banks Gene Library genes Genes. Genome Genetic Markers - genetics Genetics of eukaryotes. Biological and molecular evolution Genome/plastome incompatibility Interspecific genome/plastome hybrids and cybrids Molecular and cellular biology molecular cloning Molecular genetics molecular sequence data nucleotide sequences Oenothera Oenothera - genetics Oenothera elata Plant Infertility - genetics Plastids - genetics |
| title | Construction, database integration, and application of an Oenothera EST library |
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