Fetal spleen stroma drives macrophage commitment
The role of the fetal spleen in hematopoeisis remains largely unknown. In this particular environment, we show that hematopoietic stem cells do not proliferate, but that they lose multipotency and differentiate exclusively into mature macrophages. B lymphocytes in the spleen derive from committed B...
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| Veröffentlicht in: | Development (Cambridge) 2006-09, Vol.133 (18), p.3619-3628 |
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| container_title | Development (Cambridge) |
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| creator | Bertrand, Julien Y. Desanti, Guillaume E. Lo-Man, Richard Leclerc, Claude Cumano, Ana Golub, Rachel |
| description | The role of the fetal spleen in hematopoeisis remains largely unknown. In this particular environment, we show that hematopoietic stem cells do not proliferate, but that they lose multipotency and differentiate exclusively into mature macrophages. B lymphocytes in the spleen derive from committed B cell precursors that are likely to have immigrated from the fetal liver. We developed fetal spleen stromal cell lines that are unique in their capacity to expand myeloid precursors, resulting in large numbers of mature macrophages. These lines secrete high levels of anti-inflammatory molecules. By phenotype, fetal splenic macrophages are reminiscent of their adult counterparts found in the red pulp. We postulate that F4/80 + splenic macrophages participate in fetal erythropoiesis, as well as in the formation of the splenic architecture. |
| doi_str_mv | 10.1242/dev.02510 |
| format | Article |
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In this particular environment, we show that hematopoietic stem cells do not proliferate, but that they lose multipotency and differentiate exclusively into mature macrophages. B lymphocytes in the spleen derive from committed B cell precursors that are likely to have immigrated from the fetal liver. We developed fetal spleen stromal cell lines that are unique in their capacity to expand myeloid precursors, resulting in large numbers of mature macrophages. These lines secrete high levels of anti-inflammatory molecules. By phenotype, fetal splenic macrophages are reminiscent of their adult counterparts found in the red pulp. 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In this particular environment, we show that hematopoietic stem cells do not proliferate, but that they lose multipotency and differentiate exclusively into mature macrophages. B lymphocytes in the spleen derive from committed B cell precursors that are likely to have immigrated from the fetal liver. We developed fetal spleen stromal cell lines that are unique in their capacity to expand myeloid precursors, resulting in large numbers of mature macrophages. These lines secrete high levels of anti-inflammatory molecules. By phenotype, fetal splenic macrophages are reminiscent of their adult counterparts found in the red pulp. 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In this particular environment, we show that hematopoietic stem cells do not proliferate, but that they lose multipotency and differentiate exclusively into mature macrophages. B lymphocytes in the spleen derive from committed B cell precursors that are likely to have immigrated from the fetal liver. We developed fetal spleen stromal cell lines that are unique in their capacity to expand myeloid precursors, resulting in large numbers of mature macrophages. These lines secrete high levels of anti-inflammatory molecules. By phenotype, fetal splenic macrophages are reminiscent of their adult counterparts found in the red pulp. We postulate that F4/80 + splenic macrophages participate in fetal erythropoiesis, as well as in the formation of the splenic architecture.</abstract><cop>England</cop><pub>The Company of Biologists Limited</pub><pmid>16914502</pmid><doi>10.1242/dev.02510</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
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| ispartof | Development (Cambridge), 2006-09, Vol.133 (18), p.3619-3628 |
| issn | 0950-1991 1477-9129 |
| language | eng |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection; Company of Biologists |
| subjects | Animals Anti-Inflammatory Agents - pharmacology B-Lymphocytes - cytology B-Lymphocytes - metabolism Bone Marrow Cells - cytology Bone Marrow Cells - drug effects Bone Marrow Cells - metabolism Cell Differentiation - drug effects Cell Differentiation - genetics Cell Differentiation - physiology Cell Lineage - genetics Cell Lineage - physiology Cell Proliferation - drug effects Cells, Cultured Fetus Flow Cytometry - methods Hematopoietic Stem Cells - cytology Hematopoietic Stem Cells - metabolism Hematopoietic Stem Cells - physiology Immunohistochemistry Leukocyte Common Antigens - genetics Leukocyte Common Antigens - metabolism Liver - cytology Liver - metabolism Macrophages - cytology Macrophages - metabolism Mice Mice, Inbred C57BL Reverse Transcriptase Polymerase Chain Reaction Spleen - cytology Spleen - embryology Spleen - metabolism Stromal Cells - cytology Stromal Cells - metabolism T-Lymphocytes - cytology T-Lymphocytes - drug effects T-Lymphocytes - metabolism |
| title | Fetal spleen stroma drives macrophage commitment |
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