Production and characterization of a complete set of individual chromosome additions from Oryza officinalis to Oryza sativa using RFLP and GISH analyses
Monosomic alien addition lines (MAALs) are valuable materials for comparative analyses of two distinct genomes, for elucidating introgression mechanisms, and for dissecting genes controlling complex traits. In the study reported here, MAALs of rice containing the complete genome of Oryza sativa and...
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| Veröffentlicht in: | Theoretical and applied genetics 2005-11, Vol.111 (8), p.1585-1595 |
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| creator | Tan, G Jin, H Li, G He, R Zhu, L He, G |
| description | Monosomic alien addition lines (MAALs) are valuable materials for comparative analyses of two distinct genomes, for elucidating introgression mechanisms, and for dissecting genes controlling complex traits. In the study reported here, MAALs of rice containing the complete genome of Oryza sativa and individual chromosomes of Oryza officinalis were produced. Interspecific hybridizations were made between O. sativa L. ssp. Japonica (CV, Hejiang 19, 2 n=24, AA) and O. officinalis (Acc. HY018, 2 n=24, CC). Two backcrosses were made to the cultivated rice to obtain BC2F1 plants. Through RFLP and GISH analyses, 25 MAALs (2 n=25, AA+1C) were identified and divided into 12 syntenic groups, designated MAALs 1-12. MAALs 1, 2, 3, 5, 7 and 10 were each represented by one plant, MAALs 8, 11 and 12 by two plants, MAALs 6 and 9 by four plants, and MAAL 4 by five plants. An ideogram of the C-genome of O. officinalis was constructed, based on GISH analysis of the interspecific hybrid and the MAALs. Comparative RFLP maps showed strong syntenic associations between the A-genomes and C-genomes. Chromosomal arrangements such as translocations and duplications were detected in different alien chromosomes of the MAALs. The complete set of O. officinalis MAALs generated here provides a novel manipulation platform for exploiting and utilizing the O. officinalis genome and carrying out genetic studies. |
| doi_str_mv | 10.1007/s00122-005-0090-4 |
| format | Article |
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In the study reported here, MAALs of rice containing the complete genome of Oryza sativa and individual chromosomes of Oryza officinalis were produced. Interspecific hybridizations were made between O. sativa L. ssp. Japonica (CV, Hejiang 19, 2 n=24, AA) and O. officinalis (Acc. HY018, 2 n=24, CC). Two backcrosses were made to the cultivated rice to obtain BC2F1 plants. Through RFLP and GISH analyses, 25 MAALs (2 n=25, AA+1C) were identified and divided into 12 syntenic groups, designated MAALs 1-12. MAALs 1, 2, 3, 5, 7 and 10 were each represented by one plant, MAALs 8, 11 and 12 by two plants, MAALs 6 and 9 by four plants, and MAAL 4 by five plants. An ideogram of the C-genome of O. officinalis was constructed, based on GISH analysis of the interspecific hybrid and the MAALs. Comparative RFLP maps showed strong syntenic associations between the A-genomes and C-genomes. Chromosomal arrangements such as translocations and duplications were detected in different alien chromosomes of the MAALs. The complete set of O. officinalis MAALs generated here provides a novel manipulation platform for exploiting and utilizing the O. officinalis genome and carrying out genetic studies.</description><identifier>ISSN: 0040-5752</identifier><identifier>EISSN: 1432-2242</identifier><identifier>DOI: 10.1007/s00122-005-0090-4</identifier><identifier>PMID: 16177899</identifier><identifier>CODEN: THAGA6</identifier><language>eng</language><publisher>Heidelberg: Springer</publisher><subject>Biological and medical sciences ; Blotting, Southern ; Breeding - methods ; chromosome addition ; chromosome duplication ; Chromosome Mapping ; chromosome morphology ; chromosome translocation ; Chromosomes, Plant - genetics ; Classical genetics, quantitative genetics, hybrids ; Crosses, Genetic ; cytogenetics ; Fundamental and applied biological sciences. Psychology ; Gene Transfer Techniques ; genetic markers ; Genetics of eukaryotes. Biological and molecular evolution ; genome ; genomic in situ hybridization ; Genomics ; Hybridization, Genetic ; In Situ Hybridization ; interspecific hybridization ; lines ; monosomic alien addition lines ; Oryza - genetics ; Oryza officinalis ; Oryza sativa ; Phenotype ; Polymorphism, Restriction Fragment Length ; Pteridophyta, spermatophyta ; restriction fragment length polymorphism ; Rice ; Species Specificity ; synteny ; Synteny - genetics ; Vegetals</subject><ispartof>Theoretical and applied genetics, 2005-11, Vol.111 (8), p.1585-1595</ispartof><rights>2006 INIST-CNRS</rights><rights>Springer-Verlag 2005</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c411t-7a77fcb71f9e6afc8eee68e185df565a8278285c0feb99dc427ba234676ae1b73</citedby><cites>FETCH-LOGICAL-c411t-7a77fcb71f9e6afc8eee68e185df565a8278285c0feb99dc427ba234676ae1b73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17280189$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16177899$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tan, G</creatorcontrib><creatorcontrib>Jin, H</creatorcontrib><creatorcontrib>Li, G</creatorcontrib><creatorcontrib>He, R</creatorcontrib><creatorcontrib>Zhu, L</creatorcontrib><creatorcontrib>He, G</creatorcontrib><title>Production and characterization of a complete set of individual chromosome additions from Oryza officinalis to Oryza sativa using RFLP and GISH analyses</title><title>Theoretical and applied genetics</title><addtitle>Theor Appl Genet</addtitle><description>Monosomic alien addition lines (MAALs) are valuable materials for comparative analyses of two distinct genomes, for elucidating introgression mechanisms, and for dissecting genes controlling complex traits. In the study reported here, MAALs of rice containing the complete genome of Oryza sativa and individual chromosomes of Oryza officinalis were produced. Interspecific hybridizations were made between O. sativa L. ssp. Japonica (CV, Hejiang 19, 2 n=24, AA) and O. officinalis (Acc. HY018, 2 n=24, CC). Two backcrosses were made to the cultivated rice to obtain BC2F1 plants. Through RFLP and GISH analyses, 25 MAALs (2 n=25, AA+1C) were identified and divided into 12 syntenic groups, designated MAALs 1-12. MAALs 1, 2, 3, 5, 7 and 10 were each represented by one plant, MAALs 8, 11 and 12 by two plants, MAALs 6 and 9 by four plants, and MAAL 4 by five plants. An ideogram of the C-genome of O. officinalis was constructed, based on GISH analysis of the interspecific hybrid and the MAALs. Comparative RFLP maps showed strong syntenic associations between the A-genomes and C-genomes. Chromosomal arrangements such as translocations and duplications were detected in different alien chromosomes of the MAALs. The complete set of O. officinalis MAALs generated here provides a novel manipulation platform for exploiting and utilizing the O. officinalis genome and carrying out genetic studies.</description><subject>Biological and medical sciences</subject><subject>Blotting, Southern</subject><subject>Breeding - methods</subject><subject>chromosome addition</subject><subject>chromosome duplication</subject><subject>Chromosome Mapping</subject><subject>chromosome morphology</subject><subject>chromosome translocation</subject><subject>Chromosomes, Plant - genetics</subject><subject>Classical genetics, quantitative genetics, hybrids</subject><subject>Crosses, Genetic</subject><subject>cytogenetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Transfer Techniques</subject><subject>genetic markers</subject><subject>Genetics of eukaryotes. Biological and molecular evolution</subject><subject>genome</subject><subject>genomic in situ hybridization</subject><subject>Genomics</subject><subject>Hybridization, Genetic</subject><subject>In Situ Hybridization</subject><subject>interspecific hybridization</subject><subject>lines</subject><subject>monosomic alien addition lines</subject><subject>Oryza - genetics</subject><subject>Oryza officinalis</subject><subject>Oryza sativa</subject><subject>Phenotype</subject><subject>Polymorphism, Restriction Fragment Length</subject><subject>Pteridophyta, spermatophyta</subject><subject>restriction fragment length polymorphism</subject><subject>Rice</subject><subject>Species Specificity</subject><subject>synteny</subject><subject>Synteny - genetics</subject><subject>Vegetals</subject><issn>0040-5752</issn><issn>1432-2242</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFkc1q3TAQhUVpaW7TPkA3rSi0O7cjWbbkZQjND1xIaJq1GMtSqmBbt5IduHmSPm7lXEOgmy4GDYfvnEEcQt4z-MoA5LcEwDgvAKo8DRTiBdkwUfKCc8Ffkg2AgKKSFT8ib1K6BwBeQfmaHLGaSamaZkP-XMfQzWbyYaQ4dtT8wohmstE_4pMYHEVqwrDr7WRpstOi-LHzD76bsc-GGIaQwmApdp1fPIm6rNGruH_ETDtv_Ii9T3QKq5hy9gPSOfnxjv44214_3T6_vLnIC_b7ZNNb8sphn-y79T0mt2fff55eFNur88vTk21hBGNTIVFKZ1rJXGNrdEZZa2tlmao6V9UVKi4VV5UBZ9um6YzgskVeilrWaFkry2Py5ZC7i-H3bNOkB5-M7XscbZiTrpVsBK_Zf0EmOZSSiwx--ge8D3PM30pacWhKUJxniB0gE0NK0Tq9i37AuNcM9FKuPpSrc7l6KVcvwR_W4LkdbPfsWNvMwOcVwGSwdxFH49MzJ7kCphbu44FzGDTexczc3nBgJTCoGiZY-Rdal7eE</recordid><startdate>20051101</startdate><enddate>20051101</enddate><creator>Tan, G</creator><creator>Jin, H</creator><creator>Li, G</creator><creator>He, R</creator><creator>Zhu, L</creator><creator>He, G</creator><general>Springer</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7SS</scope><scope>7TK</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20051101</creationdate><title>Production and characterization of a complete set of individual chromosome additions from Oryza officinalis to Oryza sativa using RFLP and GISH analyses</title><author>Tan, G ; Jin, H ; Li, G ; He, R ; Zhu, L ; He, G</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c411t-7a77fcb71f9e6afc8eee68e185df565a8278285c0feb99dc427ba234676ae1b73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Biological and medical sciences</topic><topic>Blotting, Southern</topic><topic>Breeding - methods</topic><topic>chromosome addition</topic><topic>chromosome duplication</topic><topic>Chromosome Mapping</topic><topic>chromosome morphology</topic><topic>chromosome translocation</topic><topic>Chromosomes, Plant - genetics</topic><topic>Classical genetics, quantitative genetics, hybrids</topic><topic>Crosses, Genetic</topic><topic>cytogenetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Transfer Techniques</topic><topic>genetic markers</topic><topic>Genetics of eukaryotes. Biological and molecular evolution</topic><topic>genome</topic><topic>genomic in situ hybridization</topic><topic>Genomics</topic><topic>Hybridization, Genetic</topic><topic>In Situ Hybridization</topic><topic>interspecific hybridization</topic><topic>lines</topic><topic>monosomic alien addition lines</topic><topic>Oryza - genetics</topic><topic>Oryza officinalis</topic><topic>Oryza sativa</topic><topic>Phenotype</topic><topic>Polymorphism, Restriction Fragment Length</topic><topic>Pteridophyta, spermatophyta</topic><topic>restriction fragment length polymorphism</topic><topic>Rice</topic><topic>Species Specificity</topic><topic>synteny</topic><topic>Synteny - genetics</topic><topic>Vegetals</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tan, G</creatorcontrib><creatorcontrib>Jin, H</creatorcontrib><creatorcontrib>Li, G</creatorcontrib><creatorcontrib>He, R</creatorcontrib><creatorcontrib>Zhu, L</creatorcontrib><creatorcontrib>He, G</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Neurosciences Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Theoretical and applied genetics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tan, G</au><au>Jin, H</au><au>Li, G</au><au>He, R</au><au>Zhu, L</au><au>He, G</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Production and characterization of a complete set of individual chromosome additions from Oryza officinalis to Oryza sativa using RFLP and GISH analyses</atitle><jtitle>Theoretical and applied genetics</jtitle><addtitle>Theor Appl Genet</addtitle><date>2005-11-01</date><risdate>2005</risdate><volume>111</volume><issue>8</issue><spage>1585</spage><epage>1595</epage><pages>1585-1595</pages><issn>0040-5752</issn><eissn>1432-2242</eissn><coden>THAGA6</coden><abstract>Monosomic alien addition lines (MAALs) are valuable materials for comparative analyses of two distinct genomes, for elucidating introgression mechanisms, and for dissecting genes controlling complex traits. In the study reported here, MAALs of rice containing the complete genome of Oryza sativa and individual chromosomes of Oryza officinalis were produced. Interspecific hybridizations were made between O. sativa L. ssp. Japonica (CV, Hejiang 19, 2 n=24, AA) and O. officinalis (Acc. HY018, 2 n=24, CC). Two backcrosses were made to the cultivated rice to obtain BC2F1 plants. Through RFLP and GISH analyses, 25 MAALs (2 n=25, AA+1C) were identified and divided into 12 syntenic groups, designated MAALs 1-12. MAALs 1, 2, 3, 5, 7 and 10 were each represented by one plant, MAALs 8, 11 and 12 by two plants, MAALs 6 and 9 by four plants, and MAAL 4 by five plants. An ideogram of the C-genome of O. officinalis was constructed, based on GISH analysis of the interspecific hybrid and the MAALs. Comparative RFLP maps showed strong syntenic associations between the A-genomes and C-genomes. Chromosomal arrangements such as translocations and duplications were detected in different alien chromosomes of the MAALs. The complete set of O. officinalis MAALs generated here provides a novel manipulation platform for exploiting and utilizing the O. officinalis genome and carrying out genetic studies.</abstract><cop>Heidelberg</cop><cop>Berlin</cop><pub>Springer</pub><pmid>16177899</pmid><doi>10.1007/s00122-005-0090-4</doi><tpages>11</tpages></addata></record> |
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| subjects | Biological and medical sciences Blotting, Southern Breeding - methods chromosome addition chromosome duplication Chromosome Mapping chromosome morphology chromosome translocation Chromosomes, Plant - genetics Classical genetics, quantitative genetics, hybrids Crosses, Genetic cytogenetics Fundamental and applied biological sciences. Psychology Gene Transfer Techniques genetic markers Genetics of eukaryotes. Biological and molecular evolution genome genomic in situ hybridization Genomics Hybridization, Genetic In Situ Hybridization interspecific hybridization lines monosomic alien addition lines Oryza - genetics Oryza officinalis Oryza sativa Phenotype Polymorphism, Restriction Fragment Length Pteridophyta, spermatophyta restriction fragment length polymorphism Rice Species Specificity synteny Synteny - genetics Vegetals |
| title | Production and characterization of a complete set of individual chromosome additions from Oryza officinalis to Oryza sativa using RFLP and GISH analyses |
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