Dynamic changes in mitochondrial biogenesis and antioxidant enzymes during the spontaneous differentiation of human embryonic stem cells
Embryonic cells before implantation are exposed to a hypoxic condition and dependent on anaerobic metabolism. Human embryonic stem cells (HESCs) derived from pre-implantation blastocyst also grow well in hypoxic conditions. Expecting that the differentiating HESCs might mimic anaerobic-to-aerobic me...
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| Veröffentlicht in: | Biochemical and biophysical research communications 2006-10, Vol.348 (4), p.1472-1478 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Cho, Young Min Kwon, Sujin Pak, Youngmi Kim Seol, Hye Won Choi, Young Min Park, Do Joon Park, Kyong Soo Lee, Hong Kyu |
| description | Embryonic cells before implantation are exposed to a hypoxic condition and dependent on anaerobic metabolism. Human embryonic stem cells (HESCs) derived from pre-implantation blastocyst also grow well in hypoxic conditions. Expecting that the differentiating HESCs might mimic anaerobic-to-aerobic metabolic transition of the early human life, we examined the mitochondria-related changes in these cells. We observed that mitochondrial mass and mitochondrial DNA content were increased with differentiation, which was accompanied by the increase of the amount of ATP (4-fold) and its by-product reactive oxygen species (2.5-fold). The expression of various antioxidant enzymes including mitochondrial and cytoplasmic superoxide dismutases, catalase, and peroxiredoxins showed a dramatic change during the early differentiation. In conclusion, HESC differentiation was followed by dynamic changes in mitochondrial mass, ATP and ROS production, and antioxidant enzyme expressions. Therefore, the HESCs would serve as a good model to examine the mitochondrial biology during the early human differentiation. |
| doi_str_mv | 10.1016/j.bbrc.2006.08.020 |
| format | Article |
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Human embryonic stem cells (HESCs) derived from pre-implantation blastocyst also grow well in hypoxic conditions. Expecting that the differentiating HESCs might mimic anaerobic-to-aerobic metabolic transition of the early human life, we examined the mitochondria-related changes in these cells. We observed that mitochondrial mass and mitochondrial DNA content were increased with differentiation, which was accompanied by the increase of the amount of ATP (4-fold) and its by-product reactive oxygen species (2.5-fold). The expression of various antioxidant enzymes including mitochondrial and cytoplasmic superoxide dismutases, catalase, and peroxiredoxins showed a dramatic change during the early differentiation. In conclusion, HESC differentiation was followed by dynamic changes in mitochondrial mass, ATP and ROS production, and antioxidant enzyme expressions. Therefore, the HESCs would serve as a good model to examine the mitochondrial biology during the early human differentiation.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/j.bbrc.2006.08.020</identifier><identifier>PMID: 16920071</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Adenosine Triphosphate - metabolism ; Antioxidant ; Antioxidants - metabolism ; Cell Differentiation ; Cell Line ; DNA, Mitochondrial - metabolism ; Embryo, Mammalian - cytology ; Embryonic stem cell ; Enzymes - metabolism ; Humans ; Mitochondria ; Mitochondria - metabolism ; Mitochondria - ultrastructure ; Mitochondrial Proteins - metabolism ; Oxidative Phosphorylation ; Reactive oxygen species ; Reactive Oxygen Species - metabolism ; Stem Cells - cytology ; Stem Cells - enzymology ; Stem Cells - metabolism</subject><ispartof>Biochemical and biophysical research communications, 2006-10, Vol.348 (4), p.1472-1478</ispartof><rights>2006 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c451t-b0bbfa0549cc263b515bd95b2092ce5dbbe10079b22edc30654979f675a2dd6f3</citedby><cites>FETCH-LOGICAL-c451t-b0bbfa0549cc263b515bd95b2092ce5dbbe10079b22edc30654979f675a2dd6f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.bbrc.2006.08.020$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,45974</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16920071$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cho, Young Min</creatorcontrib><creatorcontrib>Kwon, Sujin</creatorcontrib><creatorcontrib>Pak, Youngmi Kim</creatorcontrib><creatorcontrib>Seol, Hye Won</creatorcontrib><creatorcontrib>Choi, Young Min</creatorcontrib><creatorcontrib>Park, Do Joon</creatorcontrib><creatorcontrib>Park, Kyong Soo</creatorcontrib><creatorcontrib>Lee, Hong Kyu</creatorcontrib><title>Dynamic changes in mitochondrial biogenesis and antioxidant enzymes during the spontaneous differentiation of human embryonic stem cells</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>Embryonic cells before implantation are exposed to a hypoxic condition and dependent on anaerobic metabolism. Human embryonic stem cells (HESCs) derived from pre-implantation blastocyst also grow well in hypoxic conditions. Expecting that the differentiating HESCs might mimic anaerobic-to-aerobic metabolic transition of the early human life, we examined the mitochondria-related changes in these cells. We observed that mitochondrial mass and mitochondrial DNA content were increased with differentiation, which was accompanied by the increase of the amount of ATP (4-fold) and its by-product reactive oxygen species (2.5-fold). The expression of various antioxidant enzymes including mitochondrial and cytoplasmic superoxide dismutases, catalase, and peroxiredoxins showed a dramatic change during the early differentiation. In conclusion, HESC differentiation was followed by dynamic changes in mitochondrial mass, ATP and ROS production, and antioxidant enzyme expressions. Therefore, the HESCs would serve as a good model to examine the mitochondrial biology during the early human differentiation.</description><subject>Adenosine Triphosphate - metabolism</subject><subject>Antioxidant</subject><subject>Antioxidants - metabolism</subject><subject>Cell Differentiation</subject><subject>Cell Line</subject><subject>DNA, Mitochondrial - metabolism</subject><subject>Embryo, Mammalian - cytology</subject><subject>Embryonic stem cell</subject><subject>Enzymes - metabolism</subject><subject>Humans</subject><subject>Mitochondria</subject><subject>Mitochondria - metabolism</subject><subject>Mitochondria - ultrastructure</subject><subject>Mitochondrial Proteins - metabolism</subject><subject>Oxidative Phosphorylation</subject><subject>Reactive oxygen species</subject><subject>Reactive Oxygen Species - metabolism</subject><subject>Stem Cells - cytology</subject><subject>Stem Cells - enzymology</subject><subject>Stem Cells - metabolism</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkctqGzEUhkVJadykL9BF0Cq7mR5pPBoLsinpFQLdtJCd0OWMLWNJjjRT6j5BH7syNmTXLsQB6fuOjvQT8pZBy4CJd9vWmGxbDiBaWLXA4QVZMJDQcAbLC7KAetJwyR4vyetStgCMLYV8RS6ZkNUa2IL8-XCIOnhL7UbHNRbqIw1-SnaToste76jxaY0Riy9UR1fX5NMv72qlGH8fQnXcnH1c02mDtOxTnHTENNdtP46YsQq6OpGmkW7moCPFYPIhxXprmTBQi7tduSYvR70r-OZcr8iPTx-_339pHr59_nr__qGxy55NjQFjRg39UlrLRWd61hsne8NBcou9MwZZfZk0nKOzHYhKDnIUQ6-5c2Lsrsjtqe8-p6cZy6SCL8cJTkMrsRpkBz3_L8jkMLCOywryE2hzKiXjqPbZB50PioE6BqW26hiUOgalYKVqUFW6OXefTUD3rJyTqcDdCcD6GT89ZlWsx2jR-Yx2Ui75f_X_C0odqCY</recordid><startdate>20061006</startdate><enddate>20061006</enddate><creator>Cho, Young Min</creator><creator>Kwon, Sujin</creator><creator>Pak, Youngmi Kim</creator><creator>Seol, Hye Won</creator><creator>Choi, Young Min</creator><creator>Park, Do Joon</creator><creator>Park, Kyong Soo</creator><creator>Lee, Hong Kyu</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20061006</creationdate><title>Dynamic changes in mitochondrial biogenesis and antioxidant enzymes during the spontaneous differentiation of human embryonic stem cells</title><author>Cho, Young Min ; Kwon, Sujin ; Pak, Youngmi Kim ; Seol, Hye Won ; Choi, Young Min ; Park, Do Joon ; Park, Kyong Soo ; Lee, Hong Kyu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c451t-b0bbfa0549cc263b515bd95b2092ce5dbbe10079b22edc30654979f675a2dd6f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Adenosine Triphosphate - metabolism</topic><topic>Antioxidant</topic><topic>Antioxidants - metabolism</topic><topic>Cell Differentiation</topic><topic>Cell Line</topic><topic>DNA, Mitochondrial - metabolism</topic><topic>Embryo, Mammalian - cytology</topic><topic>Embryonic stem cell</topic><topic>Enzymes - metabolism</topic><topic>Humans</topic><topic>Mitochondria</topic><topic>Mitochondria - metabolism</topic><topic>Mitochondria - ultrastructure</topic><topic>Mitochondrial Proteins - metabolism</topic><topic>Oxidative Phosphorylation</topic><topic>Reactive oxygen species</topic><topic>Reactive Oxygen Species - metabolism</topic><topic>Stem Cells - cytology</topic><topic>Stem Cells - enzymology</topic><topic>Stem Cells - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cho, Young Min</creatorcontrib><creatorcontrib>Kwon, Sujin</creatorcontrib><creatorcontrib>Pak, Youngmi Kim</creatorcontrib><creatorcontrib>Seol, Hye Won</creatorcontrib><creatorcontrib>Choi, Young Min</creatorcontrib><creatorcontrib>Park, Do Joon</creatorcontrib><creatorcontrib>Park, Kyong Soo</creatorcontrib><creatorcontrib>Lee, Hong Kyu</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cho, Young Min</au><au>Kwon, Sujin</au><au>Pak, Youngmi Kim</au><au>Seol, Hye Won</au><au>Choi, Young Min</au><au>Park, Do Joon</au><au>Park, Kyong Soo</au><au>Lee, Hong Kyu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Dynamic changes in mitochondrial biogenesis and antioxidant enzymes during the spontaneous differentiation of human embryonic stem cells</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2006-10-06</date><risdate>2006</risdate><volume>348</volume><issue>4</issue><spage>1472</spage><epage>1478</epage><pages>1472-1478</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>Embryonic cells before implantation are exposed to a hypoxic condition and dependent on anaerobic metabolism. Human embryonic stem cells (HESCs) derived from pre-implantation blastocyst also grow well in hypoxic conditions. Expecting that the differentiating HESCs might mimic anaerobic-to-aerobic metabolic transition of the early human life, we examined the mitochondria-related changes in these cells. We observed that mitochondrial mass and mitochondrial DNA content were increased with differentiation, which was accompanied by the increase of the amount of ATP (4-fold) and its by-product reactive oxygen species (2.5-fold). The expression of various antioxidant enzymes including mitochondrial and cytoplasmic superoxide dismutases, catalase, and peroxiredoxins showed a dramatic change during the early differentiation. In conclusion, HESC differentiation was followed by dynamic changes in mitochondrial mass, ATP and ROS production, and antioxidant enzyme expressions. 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| subjects | Adenosine Triphosphate - metabolism Antioxidant Antioxidants - metabolism Cell Differentiation Cell Line DNA, Mitochondrial - metabolism Embryo, Mammalian - cytology Embryonic stem cell Enzymes - metabolism Humans Mitochondria Mitochondria - metabolism Mitochondria - ultrastructure Mitochondrial Proteins - metabolism Oxidative Phosphorylation Reactive oxygen species Reactive Oxygen Species - metabolism Stem Cells - cytology Stem Cells - enzymology Stem Cells - metabolism |
| title | Dynamic changes in mitochondrial biogenesis and antioxidant enzymes during the spontaneous differentiation of human embryonic stem cells |
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