Expression of growth hormone-releasing hormone (GHRH) and splice variant of GHRH receptors in normal mouse tissues
Growth hormone-releasing hormone (GHRH) stimulates the production and release of growth hormone in the pituitary and induces cell proliferation in a variety of peripheral tissues and tumors. These extrapituitary effects of GHRH are in many cases mediated by a splice variant of GHRH receptor designat...
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| Veröffentlicht in: | Regulatory peptides 2006-09, Vol.136 (1), p.105-108 |
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| creator | Christodoulou, C. Schally, A.V. Chatzistamou, I. Kondi-Pafiti, A. Lamnissou, K. Kouloheri, S. Kalofoutis, A. Kiaris, H. |
| description | Growth hormone-releasing hormone (GHRH) stimulates the production and release of growth hormone in the pituitary and induces cell proliferation in a variety of peripheral tissues and tumors. These extrapituitary effects of GHRH are in many cases mediated by a splice variant of GHRH receptor designated SV1 that differs from the pituitary GHRH receptor in a small portion of its amino-terminal region. While SV1 has been detected in several primary tumors and many cancer cell lines its expression in normal tissues remains unclear. In this study we report the results of an immunohistochemical analysis for SV1 and GHRH expression in normal mouse tissues. For the detection of SV1 immunoreactivity we used a polyclonal antiserum against segments 1–25 of the SV1 receptor protein. Mouse heart, colon, lungs, small intestine, stomach and kidneys exhibited increased SV1 immunoreactivity. These tissues were also positive for GHRH expression, however, tissues such as the endometrium were positive only for GHRH and not for SV1 expression. On the contrary, testis were positive for SV1 and not for GHRH expression. These results indicate that SV1 may play a role in normal physiology. |
| doi_str_mv | 10.1016/j.regpep.2006.05.001 |
| format | Article |
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These extrapituitary effects of GHRH are in many cases mediated by a splice variant of GHRH receptor designated SV1 that differs from the pituitary GHRH receptor in a small portion of its amino-terminal region. While SV1 has been detected in several primary tumors and many cancer cell lines its expression in normal tissues remains unclear. In this study we report the results of an immunohistochemical analysis for SV1 and GHRH expression in normal mouse tissues. For the detection of SV1 immunoreactivity we used a polyclonal antiserum against segments 1–25 of the SV1 receptor protein. Mouse heart, colon, lungs, small intestine, stomach and kidneys exhibited increased SV1 immunoreactivity. These tissues were also positive for GHRH expression, however, tissues such as the endometrium were positive only for GHRH and not for SV1 expression. On the contrary, testis were positive for SV1 and not for GHRH expression. 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Psychology ; Gene Expression Regulation ; GHRH ; Growth Hormone-Releasing Hormone - biosynthesis ; Immunohistochemistry ; Mice ; Mice, Inbred C3H ; Mice, Inbred C57BL ; Paracrine ; Receptor ; Receptors, Neuropeptide - biosynthesis ; Receptors, Neuropeptide - genetics ; Receptors, Pituitary Hormone-Regulating Hormone - biosynthesis ; Receptors, Pituitary Hormone-Regulating Hormone - genetics ; RNA, Messenger - metabolism ; SV1 ; Tissue Distribution ; Vertebrates: endocrinology</subject><ispartof>Regulatory peptides, 2006-09, Vol.136 (1), p.105-108</ispartof><rights>2006 Elsevier B.V.</rights><rights>2006 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c456t-b17c21c9d9382bb4ab2175b72adae6a283e3944e86c5ce7440c8d8a9b003a2753</citedby><cites>FETCH-LOGICAL-c456t-b17c21c9d9382bb4ab2175b72adae6a283e3944e86c5ce7440c8d8a9b003a2753</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.regpep.2006.05.001$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18103158$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16781787$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Christodoulou, C.</creatorcontrib><creatorcontrib>Schally, A.V.</creatorcontrib><creatorcontrib>Chatzistamou, I.</creatorcontrib><creatorcontrib>Kondi-Pafiti, A.</creatorcontrib><creatorcontrib>Lamnissou, K.</creatorcontrib><creatorcontrib>Kouloheri, S.</creatorcontrib><creatorcontrib>Kalofoutis, A.</creatorcontrib><creatorcontrib>Kiaris, H.</creatorcontrib><title>Expression of growth hormone-releasing hormone (GHRH) and splice variant of GHRH receptors in normal mouse tissues</title><title>Regulatory peptides</title><addtitle>Regul Pept</addtitle><description>Growth hormone-releasing hormone (GHRH) stimulates the production and release of growth hormone in the pituitary and induces cell proliferation in a variety of peripheral tissues and tumors. These extrapituitary effects of GHRH are in many cases mediated by a splice variant of GHRH receptor designated SV1 that differs from the pituitary GHRH receptor in a small portion of its amino-terminal region. While SV1 has been detected in several primary tumors and many cancer cell lines its expression in normal tissues remains unclear. In this study we report the results of an immunohistochemical analysis for SV1 and GHRH expression in normal mouse tissues. For the detection of SV1 immunoreactivity we used a polyclonal antiserum against segments 1–25 of the SV1 receptor protein. Mouse heart, colon, lungs, small intestine, stomach and kidneys exhibited increased SV1 immunoreactivity. These tissues were also positive for GHRH expression, however, tissues such as the endometrium were positive only for GHRH and not for SV1 expression. On the contrary, testis were positive for SV1 and not for GHRH expression. These results indicate that SV1 may play a role in normal physiology.</description><subject>Alternative Splicing</subject><subject>Animals</subject><subject>Autocrine</subject><subject>Biological and medical sciences</subject><subject>Extrapituitary</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation</subject><subject>GHRH</subject><subject>Growth Hormone-Releasing Hormone - biosynthesis</subject><subject>Immunohistochemistry</subject><subject>Mice</subject><subject>Mice, Inbred C3H</subject><subject>Mice, Inbred C57BL</subject><subject>Paracrine</subject><subject>Receptor</subject><subject>Receptors, Neuropeptide - biosynthesis</subject><subject>Receptors, Neuropeptide - genetics</subject><subject>Receptors, Pituitary Hormone-Regulating Hormone - biosynthesis</subject><subject>Receptors, Pituitary Hormone-Regulating Hormone - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>SV1</subject><subject>Tissue Distribution</subject><subject>Vertebrates: endocrinology</subject><issn>0167-0115</issn><issn>1873-1686</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMFq3DAQhkVoSLZp3iAUXRragx1Jtiz5EgghyQYChZKehSzPbrTYkqvxJu3bV8tuya2ngZnvnxk-Qi44KznjzdWmTLCeYCoFY03JZMkYPyILrlVV8EY3H8giY6pgnMtT8hFxkwGpVHVCTnNfc6XVgqS731MCRB8DjSu6TvFtfqEvMY0xQJFgAIs-rP916NeH5Y_lN2pDT3EavAP6apO3Yd6ldzOawME0x4TUBxpyzA50jFsEOnvELeAncryyA8L5oZ6Rn_d3z7fL4un7w-PtzVPhatnMRceVE9y1fVtp0XW17QRXslPC9hYaK3QFVVvXoBsnHai6Zk732rYdY5UVSlZn5HK_d0rxV747m9Gjg2GwAfI_ptGqFVrUGaz3oEsRMcHKTMmPNv0xnJmda7Mxe9dm59owabLKHPt82L_tRujfQwe5GfhyACw6O6ySDc7jO6c5q7jUmbvec5BtvHpIBp2H4KD3WeZs-uj__8lfKy2foA</recordid><startdate>20060911</startdate><enddate>20060911</enddate><creator>Christodoulou, C.</creator><creator>Schally, A.V.</creator><creator>Chatzistamou, I.</creator><creator>Kondi-Pafiti, A.</creator><creator>Lamnissou, K.</creator><creator>Kouloheri, S.</creator><creator>Kalofoutis, A.</creator><creator>Kiaris, H.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20060911</creationdate><title>Expression of growth hormone-releasing hormone (GHRH) and splice variant of GHRH receptors in normal mouse tissues</title><author>Christodoulou, C. ; Schally, A.V. ; Chatzistamou, I. ; Kondi-Pafiti, A. ; Lamnissou, K. ; Kouloheri, S. ; Kalofoutis, A. ; Kiaris, H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c456t-b17c21c9d9382bb4ab2175b72adae6a283e3944e86c5ce7440c8d8a9b003a2753</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Alternative Splicing</topic><topic>Animals</topic><topic>Autocrine</topic><topic>Biological and medical sciences</topic><topic>Extrapituitary</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation</topic><topic>GHRH</topic><topic>Growth Hormone-Releasing Hormone - biosynthesis</topic><topic>Immunohistochemistry</topic><topic>Mice</topic><topic>Mice, Inbred C3H</topic><topic>Mice, Inbred C57BL</topic><topic>Paracrine</topic><topic>Receptor</topic><topic>Receptors, Neuropeptide - biosynthesis</topic><topic>Receptors, Neuropeptide - genetics</topic><topic>Receptors, Pituitary Hormone-Regulating Hormone - biosynthesis</topic><topic>Receptors, Pituitary Hormone-Regulating Hormone - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>SV1</topic><topic>Tissue Distribution</topic><topic>Vertebrates: endocrinology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Christodoulou, C.</creatorcontrib><creatorcontrib>Schally, A.V.</creatorcontrib><creatorcontrib>Chatzistamou, I.</creatorcontrib><creatorcontrib>Kondi-Pafiti, A.</creatorcontrib><creatorcontrib>Lamnissou, K.</creatorcontrib><creatorcontrib>Kouloheri, S.</creatorcontrib><creatorcontrib>Kalofoutis, A.</creatorcontrib><creatorcontrib>Kiaris, H.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Regulatory peptides</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Christodoulou, C.</au><au>Schally, A.V.</au><au>Chatzistamou, I.</au><au>Kondi-Pafiti, A.</au><au>Lamnissou, K.</au><au>Kouloheri, S.</au><au>Kalofoutis, A.</au><au>Kiaris, H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of growth hormone-releasing hormone (GHRH) and splice variant of GHRH receptors in normal mouse tissues</atitle><jtitle>Regulatory peptides</jtitle><addtitle>Regul Pept</addtitle><date>2006-09-11</date><risdate>2006</risdate><volume>136</volume><issue>1</issue><spage>105</spage><epage>108</epage><pages>105-108</pages><issn>0167-0115</issn><eissn>1873-1686</eissn><coden>REPPDY</coden><abstract>Growth hormone-releasing hormone (GHRH) stimulates the production and release of growth hormone in the pituitary and induces cell proliferation in a variety of peripheral tissues and tumors. These extrapituitary effects of GHRH are in many cases mediated by a splice variant of GHRH receptor designated SV1 that differs from the pituitary GHRH receptor in a small portion of its amino-terminal region. While SV1 has been detected in several primary tumors and many cancer cell lines its expression in normal tissues remains unclear. In this study we report the results of an immunohistochemical analysis for SV1 and GHRH expression in normal mouse tissues. For the detection of SV1 immunoreactivity we used a polyclonal antiserum against segments 1–25 of the SV1 receptor protein. Mouse heart, colon, lungs, small intestine, stomach and kidneys exhibited increased SV1 immunoreactivity. These tissues were also positive for GHRH expression, however, tissues such as the endometrium were positive only for GHRH and not for SV1 expression. On the contrary, testis were positive for SV1 and not for GHRH expression. 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| subjects | Alternative Splicing Animals Autocrine Biological and medical sciences Extrapituitary Female Fundamental and applied biological sciences. Psychology Gene Expression Regulation GHRH Growth Hormone-Releasing Hormone - biosynthesis Immunohistochemistry Mice Mice, Inbred C3H Mice, Inbred C57BL Paracrine Receptor Receptors, Neuropeptide - biosynthesis Receptors, Neuropeptide - genetics Receptors, Pituitary Hormone-Regulating Hormone - biosynthesis Receptors, Pituitary Hormone-Regulating Hormone - genetics RNA, Messenger - metabolism SV1 Tissue Distribution Vertebrates: endocrinology |
| title | Expression of growth hormone-releasing hormone (GHRH) and splice variant of GHRH receptors in normal mouse tissues |
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