hCDC4 variation in osteosarcoma
The hCDC4 gene (also known as Fbw7 or Archipelago) encodes an F-box protein that is responsible for targeting cyclin E for Skp1-cullin-F box protein (SCF) ubiquitination and proteosomal degradation. Disruption of this pathway has been associated with chromosomal instability and aneuploidy in several...
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| Veröffentlicht in: | Cancer genetics and cytogenetics 2006-09, Vol.169 (2), p.138-142 |
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| container_title | Cancer genetics and cytogenetics |
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| creator | Yan, Taiqiang Wunder, Jay S. Gokgoz, Nalan Seto, Kelly K.Y. Bell, Robert S. Andrulis, Irene L. |
| description | The
hCDC4 gene (also known as
Fbw7 or Archipelago) encodes an F-box protein that is responsible for targeting cyclin E for Skp1-cullin-F box protein (SCF) ubiquitination and proteosomal degradation. Disruption of this pathway has been associated with chromosomal instability and aneuploidy in several cancer cell lines and primary tumors. This study aimed to examine whether
hCDC4 mutations contribute to aneuploidy in osteosarcoma. We analyzed 147 primary high-grade osteosarcoma specimens and 6 osteosarcoma cell lines. The protein truncation test (PTT) and single-strand conformation polymorphism (SSCP) analysis with subsequent sequencing were performed to detect alterations of the
hCDC4 gene. All specimens exhibited the same PTT pattern of normal bands with less intense common bands. Two shifts were detected by SSCP, and subsequent DNA analysis identified one in-frame three-base GAG (424-426) deletion and one silent nucleotide substitution (C1261T). We conclude that somatic
hCDC4 mutations are infrequent in osteosarcoma, and are unlikely to play an important role in aneuploidy of this tumor. |
| doi_str_mv | 10.1016/j.cancergencyto.2006.04.007 |
| format | Article |
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hCDC4 gene (also known as
Fbw7 or Archipelago) encodes an F-box protein that is responsible for targeting cyclin E for Skp1-cullin-F box protein (SCF) ubiquitination and proteosomal degradation. Disruption of this pathway has been associated with chromosomal instability and aneuploidy in several cancer cell lines and primary tumors. This study aimed to examine whether
hCDC4 mutations contribute to aneuploidy in osteosarcoma. We analyzed 147 primary high-grade osteosarcoma specimens and 6 osteosarcoma cell lines. The protein truncation test (PTT) and single-strand conformation polymorphism (SSCP) analysis with subsequent sequencing were performed to detect alterations of the
hCDC4 gene. All specimens exhibited the same PTT pattern of normal bands with less intense common bands. Two shifts were detected by SSCP, and subsequent DNA analysis identified one in-frame three-base GAG (424-426) deletion and one silent nucleotide substitution (C1261T). We conclude that somatic
hCDC4 mutations are infrequent in osteosarcoma, and are unlikely to play an important role in aneuploidy of this tumor.</description><identifier>ISSN: 0165-4608</identifier><identifier>EISSN: 1873-4456</identifier><identifier>DOI: 10.1016/j.cancergencyto.2006.04.007</identifier><identifier>PMID: 16938571</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Aneuploidy ; Cell Cycle Proteins - genetics ; Cell Line, Tumor ; F-Box Proteins - genetics ; F-Box-WD Repeat-Containing Protein 7 ; Humans ; Mutation ; Osteosarcoma - genetics ; Polymorphism, Single-Stranded Conformational ; Sequence Analysis, DNA - methods ; Ubiquitin-Protein Ligases - genetics</subject><ispartof>Cancer genetics and cytogenetics, 2006-09, Vol.169 (2), p.138-142</ispartof><rights>2006 Elsevier Inc.</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c412t-8ef51d118a976d97e6b522342f57c4a26974e707be74845bd6ed3439b1681a933</citedby><cites>FETCH-LOGICAL-c412t-8ef51d118a976d97e6b522342f57c4a26974e707be74845bd6ed3439b1681a933</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0165460806002573$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16938571$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yan, Taiqiang</creatorcontrib><creatorcontrib>Wunder, Jay S.</creatorcontrib><creatorcontrib>Gokgoz, Nalan</creatorcontrib><creatorcontrib>Seto, Kelly K.Y.</creatorcontrib><creatorcontrib>Bell, Robert S.</creatorcontrib><creatorcontrib>Andrulis, Irene L.</creatorcontrib><title>hCDC4 variation in osteosarcoma</title><title>Cancer genetics and cytogenetics</title><addtitle>Cancer Genet Cytogenet</addtitle><description>The
hCDC4 gene (also known as
Fbw7 or Archipelago) encodes an F-box protein that is responsible for targeting cyclin E for Skp1-cullin-F box protein (SCF) ubiquitination and proteosomal degradation. Disruption of this pathway has been associated with chromosomal instability and aneuploidy in several cancer cell lines and primary tumors. This study aimed to examine whether
hCDC4 mutations contribute to aneuploidy in osteosarcoma. We analyzed 147 primary high-grade osteosarcoma specimens and 6 osteosarcoma cell lines. The protein truncation test (PTT) and single-strand conformation polymorphism (SSCP) analysis with subsequent sequencing were performed to detect alterations of the
hCDC4 gene. All specimens exhibited the same PTT pattern of normal bands with less intense common bands. Two shifts were detected by SSCP, and subsequent DNA analysis identified one in-frame three-base GAG (424-426) deletion and one silent nucleotide substitution (C1261T). We conclude that somatic
hCDC4 mutations are infrequent in osteosarcoma, and are unlikely to play an important role in aneuploidy of this tumor.</description><subject>Aneuploidy</subject><subject>Cell Cycle Proteins - genetics</subject><subject>Cell Line, Tumor</subject><subject>F-Box Proteins - genetics</subject><subject>F-Box-WD Repeat-Containing Protein 7</subject><subject>Humans</subject><subject>Mutation</subject><subject>Osteosarcoma - genetics</subject><subject>Polymorphism, Single-Stranded Conformational</subject><subject>Sequence Analysis, DNA - methods</subject><subject>Ubiquitin-Protein Ligases - genetics</subject><issn>0165-4608</issn><issn>1873-4456</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkEtLAzEUhYMotlb_ghYEdzMmM3niSsb6gIIbXYdM5o6mdCY1mRb6701pQVzp6m6-cw73Q-ia4Jxgwm8XuTW9hfABvd0OPi8w5jmmOcbiCI2JFGVGKePHaJxollGO5QidxbjAiSgUP0UjwlUpmSBjdPVZPVR0ujHBmcH5fur6qY8D-GiC9Z05RyetWUa4ONwJen-cvVXP2fz16aW6n2eWkmLIJLSMNIRIowRvlABes6IoadEyYakpuBIUBBY1CCopqxsOTUlLVRMuiVFlOUE3-95V8F9riIPuXLSwXJoe_DpqLoUiQrA_wQKn30suEni3B23wMQZo9Sq4zoStJljvROqF_iVS70RqTHXSlNKXh5l13UHzkz2YS8BsD0CysnEQdLQuFUHjAthBN979a-gbky2Jfg</recordid><startdate>20060901</startdate><enddate>20060901</enddate><creator>Yan, Taiqiang</creator><creator>Wunder, Jay S.</creator><creator>Gokgoz, Nalan</creator><creator>Seto, Kelly K.Y.</creator><creator>Bell, Robert S.</creator><creator>Andrulis, Irene L.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20060901</creationdate><title>hCDC4 variation in osteosarcoma</title><author>Yan, Taiqiang ; Wunder, Jay S. ; Gokgoz, Nalan ; Seto, Kelly K.Y. ; Bell, Robert S. ; Andrulis, Irene L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c412t-8ef51d118a976d97e6b522342f57c4a26974e707be74845bd6ed3439b1681a933</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Aneuploidy</topic><topic>Cell Cycle Proteins - genetics</topic><topic>Cell Line, Tumor</topic><topic>F-Box Proteins - genetics</topic><topic>F-Box-WD Repeat-Containing Protein 7</topic><topic>Humans</topic><topic>Mutation</topic><topic>Osteosarcoma - genetics</topic><topic>Polymorphism, Single-Stranded Conformational</topic><topic>Sequence Analysis, DNA - methods</topic><topic>Ubiquitin-Protein Ligases - genetics</topic><toplevel>online_resources</toplevel><creatorcontrib>Yan, Taiqiang</creatorcontrib><creatorcontrib>Wunder, Jay S.</creatorcontrib><creatorcontrib>Gokgoz, Nalan</creatorcontrib><creatorcontrib>Seto, Kelly K.Y.</creatorcontrib><creatorcontrib>Bell, Robert S.</creatorcontrib><creatorcontrib>Andrulis, Irene L.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Cancer genetics and cytogenetics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yan, Taiqiang</au><au>Wunder, Jay S.</au><au>Gokgoz, Nalan</au><au>Seto, Kelly K.Y.</au><au>Bell, Robert S.</au><au>Andrulis, Irene L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>hCDC4 variation in osteosarcoma</atitle><jtitle>Cancer genetics and cytogenetics</jtitle><addtitle>Cancer Genet Cytogenet</addtitle><date>2006-09-01</date><risdate>2006</risdate><volume>169</volume><issue>2</issue><spage>138</spage><epage>142</epage><pages>138-142</pages><issn>0165-4608</issn><eissn>1873-4456</eissn><abstract>The
hCDC4 gene (also known as
Fbw7 or Archipelago) encodes an F-box protein that is responsible for targeting cyclin E for Skp1-cullin-F box protein (SCF) ubiquitination and proteosomal degradation. Disruption of this pathway has been associated with chromosomal instability and aneuploidy in several cancer cell lines and primary tumors. This study aimed to examine whether
hCDC4 mutations contribute to aneuploidy in osteosarcoma. We analyzed 147 primary high-grade osteosarcoma specimens and 6 osteosarcoma cell lines. The protein truncation test (PTT) and single-strand conformation polymorphism (SSCP) analysis with subsequent sequencing were performed to detect alterations of the
hCDC4 gene. All specimens exhibited the same PTT pattern of normal bands with less intense common bands. Two shifts were detected by SSCP, and subsequent DNA analysis identified one in-frame three-base GAG (424-426) deletion and one silent nucleotide substitution (C1261T). We conclude that somatic
hCDC4 mutations are infrequent in osteosarcoma, and are unlikely to play an important role in aneuploidy of this tumor.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>16938571</pmid><doi>10.1016/j.cancergencyto.2006.04.007</doi><tpages>5</tpages></addata></record> |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Aneuploidy Cell Cycle Proteins - genetics Cell Line, Tumor F-Box Proteins - genetics F-Box-WD Repeat-Containing Protein 7 Humans Mutation Osteosarcoma - genetics Polymorphism, Single-Stranded Conformational Sequence Analysis, DNA - methods Ubiquitin-Protein Ligases - genetics |
| title | hCDC4 variation in osteosarcoma |
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