Evaluation of pegylated lipid nanocapsules versus complement system activation and macrophage uptake
This work consisted in defining the in vitro behavior of pegylated lipid nanocapsules (LNC) toward the immune system. LNC were composed of an oily core surrounded by a shell of lecithin and polyethylene glycol (PEG) known to decrease the recognition of nanoparticles by the immune system. The “stealt...
Gespeichert in:
| Veröffentlicht in: | Journal of biomedical materials research. Part A 2006-09, Vol.78A (3), p.620-628 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 628 |
|---|---|
| container_issue | 3 |
| container_start_page | 620 |
| container_title | Journal of biomedical materials research. Part A |
| container_volume | 78A |
| creator | Vonarbourg, A. Passirani, C. Saulnier, P. Simard, P. Leroux, J.C. Benoit, J.P. |
| description | This work consisted in defining the in vitro behavior of pegylated lipid nanocapsules (LNC) toward the immune system. LNC were composed of an oily core surrounded by a shell of lecithin and polyethylene glycol (PEG) known to decrease the recognition of nanoparticles by the immune system. The “stealth” properties were evaluated by measuring complement activation (CH50 technique and crossed‐immunoelectrophoresis (C3 cleavage)) and macrophage uptake. These experiments were performed on 20‐, 50‐, and 100‐nm LNC before and after dialysis. A high density of PEG at the surface led to very low complement activation by LNC with a slight effect of size. This size effect, associated to a dialysis effect in macrophage uptake, was due to differences in density and flexibility of PEG chains related to LNC curvature radius. Thanks to a high density, 660‐Da PEG provided LNC a steric stabilization and a protective effect versus complement protein opsonization, but this protection decreased with the increase of LNC size, especially versus macrophage uptake. © 2006 Wiley Periodicals, Inc. J Biomed Mater Res, 2006 |
| doi_str_mv | 10.1002/jbm.a.30711 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_68791727</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>68791727</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4331-f0530663b658b7e5b049b30debe8795558c09d56147fb2e94e766fd77c7718293</originalsourceid><addsrcrecordid>eNp9kDlP9DAQQC0E4q7okSsalMWOY8-6BMQpjgYEorGcZAIB5yBOlm___WfIAh3VTPHmafQI2eFswhmLD17TamInggHnS2SdSxlHiVZy-XNPdCRirdbIhvevAVZMxqtkjSsADQrWSX4ys26wfdnUtCloi89zZ3vMqSvbMqe1rZvMtn5w6OkMOz94mjVV67DCuqd-7nusqM36cjY6bJ3TymZd077YZ6RD29s33CIrhXUetxdzk9yfntwdn0dXt2cXx4dXUZYIwaOCScGUEqmS0xRQpizRqWA5pjgFLaWcZkznUvEEijRGnSAoVeQAGQCfxlpskr3R23bN-4C-N1XpM3TO1tgM3qig4RBDAPdHMDzqfYeFabuyst3ccGY-o5oQ1VjzFTXQuwvtkFaY_7KLigHgI_BROpz_5TKXR9ff0mi8KUPCfz83tnszwQjSPNycGSkuH58e5bkB8R9pEpKc</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>68791727</pqid></control><display><type>article</type><title>Evaluation of pegylated lipid nanocapsules versus complement system activation and macrophage uptake</title><source>MEDLINE</source><source>Wiley Online Library All Journals</source><creator>Vonarbourg, A. ; Passirani, C. ; Saulnier, P. ; Simard, P. ; Leroux, J.C. ; Benoit, J.P.</creator><creatorcontrib>Vonarbourg, A. ; Passirani, C. ; Saulnier, P. ; Simard, P. ; Leroux, J.C. ; Benoit, J.P.</creatorcontrib><description>This work consisted in defining the in vitro behavior of pegylated lipid nanocapsules (LNC) toward the immune system. LNC were composed of an oily core surrounded by a shell of lecithin and polyethylene glycol (PEG) known to decrease the recognition of nanoparticles by the immune system. The “stealth” properties were evaluated by measuring complement activation (CH50 technique and crossed‐immunoelectrophoresis (C3 cleavage)) and macrophage uptake. These experiments were performed on 20‐, 50‐, and 100‐nm LNC before and after dialysis. A high density of PEG at the surface led to very low complement activation by LNC with a slight effect of size. This size effect, associated to a dialysis effect in macrophage uptake, was due to differences in density and flexibility of PEG chains related to LNC curvature radius. Thanks to a high density, 660‐Da PEG provided LNC a steric stabilization and a protective effect versus complement protein opsonization, but this protection decreased with the increase of LNC size, especially versus macrophage uptake. © 2006 Wiley Periodicals, Inc. J Biomed Mater Res, 2006</description><identifier>ISSN: 1549-3296</identifier><identifier>EISSN: 1552-4965</identifier><identifier>DOI: 10.1002/jbm.a.30711</identifier><identifier>PMID: 16779767</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Animals ; Capsules ; Cell Line ; complement ; Complement Activation ; Complement System Proteins - metabolism ; lipid nanocapsules ; Lipids ; macrophage ; Macrophages - metabolism ; Mice ; Nanotechnology ; polyethylene glycol ; Polyethylene Glycols ; protein adsorption</subject><ispartof>Journal of biomedical materials research. Part A, 2006-09, Vol.78A (3), p.620-628</ispartof><rights>Copyright © 2006 Wiley Periodicals, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4331-f0530663b658b7e5b049b30debe8795558c09d56147fb2e94e766fd77c7718293</citedby><cites>FETCH-LOGICAL-c4331-f0530663b658b7e5b049b30debe8795558c09d56147fb2e94e766fd77c7718293</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjbm.a.30711$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjbm.a.30711$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16779767$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Vonarbourg, A.</creatorcontrib><creatorcontrib>Passirani, C.</creatorcontrib><creatorcontrib>Saulnier, P.</creatorcontrib><creatorcontrib>Simard, P.</creatorcontrib><creatorcontrib>Leroux, J.C.</creatorcontrib><creatorcontrib>Benoit, J.P.</creatorcontrib><title>Evaluation of pegylated lipid nanocapsules versus complement system activation and macrophage uptake</title><title>Journal of biomedical materials research. Part A</title><addtitle>J. Biomed. Mater. Res</addtitle><description>This work consisted in defining the in vitro behavior of pegylated lipid nanocapsules (LNC) toward the immune system. LNC were composed of an oily core surrounded by a shell of lecithin and polyethylene glycol (PEG) known to decrease the recognition of nanoparticles by the immune system. The “stealth” properties were evaluated by measuring complement activation (CH50 technique and crossed‐immunoelectrophoresis (C3 cleavage)) and macrophage uptake. These experiments were performed on 20‐, 50‐, and 100‐nm LNC before and after dialysis. A high density of PEG at the surface led to very low complement activation by LNC with a slight effect of size. This size effect, associated to a dialysis effect in macrophage uptake, was due to differences in density and flexibility of PEG chains related to LNC curvature radius. Thanks to a high density, 660‐Da PEG provided LNC a steric stabilization and a protective effect versus complement protein opsonization, but this protection decreased with the increase of LNC size, especially versus macrophage uptake. © 2006 Wiley Periodicals, Inc. J Biomed Mater Res, 2006</description><subject>Animals</subject><subject>Capsules</subject><subject>Cell Line</subject><subject>complement</subject><subject>Complement Activation</subject><subject>Complement System Proteins - metabolism</subject><subject>lipid nanocapsules</subject><subject>Lipids</subject><subject>macrophage</subject><subject>Macrophages - metabolism</subject><subject>Mice</subject><subject>Nanotechnology</subject><subject>polyethylene glycol</subject><subject>Polyethylene Glycols</subject><subject>protein adsorption</subject><issn>1549-3296</issn><issn>1552-4965</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kDlP9DAQQC0E4q7okSsalMWOY8-6BMQpjgYEorGcZAIB5yBOlm___WfIAh3VTPHmafQI2eFswhmLD17TamInggHnS2SdSxlHiVZy-XNPdCRirdbIhvevAVZMxqtkjSsADQrWSX4ys26wfdnUtCloi89zZ3vMqSvbMqe1rZvMtn5w6OkMOz94mjVV67DCuqd-7nusqM36cjY6bJ3TymZd077YZ6RD29s33CIrhXUetxdzk9yfntwdn0dXt2cXx4dXUZYIwaOCScGUEqmS0xRQpizRqWA5pjgFLaWcZkznUvEEijRGnSAoVeQAGQCfxlpskr3R23bN-4C-N1XpM3TO1tgM3qig4RBDAPdHMDzqfYeFabuyst3ccGY-o5oQ1VjzFTXQuwvtkFaY_7KLigHgI_BROpz_5TKXR9ff0mi8KUPCfz83tnszwQjSPNycGSkuH58e5bkB8R9pEpKc</recordid><startdate>20060901</startdate><enddate>20060901</enddate><creator>Vonarbourg, A.</creator><creator>Passirani, C.</creator><creator>Saulnier, P.</creator><creator>Simard, P.</creator><creator>Leroux, J.C.</creator><creator>Benoit, J.P.</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20060901</creationdate><title>Evaluation of pegylated lipid nanocapsules versus complement system activation and macrophage uptake</title><author>Vonarbourg, A. ; Passirani, C. ; Saulnier, P. ; Simard, P. ; Leroux, J.C. ; Benoit, J.P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4331-f0530663b658b7e5b049b30debe8795558c09d56147fb2e94e766fd77c7718293</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Animals</topic><topic>Capsules</topic><topic>Cell Line</topic><topic>complement</topic><topic>Complement Activation</topic><topic>Complement System Proteins - metabolism</topic><topic>lipid nanocapsules</topic><topic>Lipids</topic><topic>macrophage</topic><topic>Macrophages - metabolism</topic><topic>Mice</topic><topic>Nanotechnology</topic><topic>polyethylene glycol</topic><topic>Polyethylene Glycols</topic><topic>protein adsorption</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Vonarbourg, A.</creatorcontrib><creatorcontrib>Passirani, C.</creatorcontrib><creatorcontrib>Saulnier, P.</creatorcontrib><creatorcontrib>Simard, P.</creatorcontrib><creatorcontrib>Leroux, J.C.</creatorcontrib><creatorcontrib>Benoit, J.P.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of biomedical materials research. Part A</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Vonarbourg, A.</au><au>Passirani, C.</au><au>Saulnier, P.</au><au>Simard, P.</au><au>Leroux, J.C.</au><au>Benoit, J.P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of pegylated lipid nanocapsules versus complement system activation and macrophage uptake</atitle><jtitle>Journal of biomedical materials research. Part A</jtitle><addtitle>J. Biomed. Mater. Res</addtitle><date>2006-09-01</date><risdate>2006</risdate><volume>78A</volume><issue>3</issue><spage>620</spage><epage>628</epage><pages>620-628</pages><issn>1549-3296</issn><eissn>1552-4965</eissn><abstract>This work consisted in defining the in vitro behavior of pegylated lipid nanocapsules (LNC) toward the immune system. LNC were composed of an oily core surrounded by a shell of lecithin and polyethylene glycol (PEG) known to decrease the recognition of nanoparticles by the immune system. The “stealth” properties were evaluated by measuring complement activation (CH50 technique and crossed‐immunoelectrophoresis (C3 cleavage)) and macrophage uptake. These experiments were performed on 20‐, 50‐, and 100‐nm LNC before and after dialysis. A high density of PEG at the surface led to very low complement activation by LNC with a slight effect of size. This size effect, associated to a dialysis effect in macrophage uptake, was due to differences in density and flexibility of PEG chains related to LNC curvature radius. Thanks to a high density, 660‐Da PEG provided LNC a steric stabilization and a protective effect versus complement protein opsonization, but this protection decreased with the increase of LNC size, especially versus macrophage uptake. © 2006 Wiley Periodicals, Inc. J Biomed Mater Res, 2006</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>16779767</pmid><doi>10.1002/jbm.a.30711</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1549-3296 |
| ispartof | Journal of biomedical materials research. Part A, 2006-09, Vol.78A (3), p.620-628 |
| issn | 1549-3296 1552-4965 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_68791727 |
| source | MEDLINE; Wiley Online Library All Journals |
| subjects | Animals Capsules Cell Line complement Complement Activation Complement System Proteins - metabolism lipid nanocapsules Lipids macrophage Macrophages - metabolism Mice Nanotechnology polyethylene glycol Polyethylene Glycols protein adsorption |
| title | Evaluation of pegylated lipid nanocapsules versus complement system activation and macrophage uptake |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-20T17%3A43%3A14IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Evaluation%20of%20pegylated%20lipid%20nanocapsules%20versus%20complement%20system%20activation%20and%20macrophage%20uptake&rft.jtitle=Journal%20of%20biomedical%20materials%20research.%20Part%20A&rft.au=Vonarbourg,%20A.&rft.date=2006-09-01&rft.volume=78A&rft.issue=3&rft.spage=620&rft.epage=628&rft.pages=620-628&rft.issn=1549-3296&rft.eissn=1552-4965&rft_id=info:doi/10.1002/jbm.a.30711&rft_dat=%3Cproquest_cross%3E68791727%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=68791727&rft_id=info:pmid/16779767&rfr_iscdi=true |