Alternative splicing of delta-like 1 homolog ( DLK1) in the pig and human
Delta-like homolog 1 ( DLK1), a paternally imprinted gene with several alternative splicing isoforms, is an important regulator of fetal and postnatal development. We report the sequence of porcine DLK1 ( pDLK1) and examine the expression and alternative splicing isoforms in the pig ( Sus scrofa) an...
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| Veröffentlicht in: | Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology 2006-09, Vol.145 (1), p.50-59 |
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| container_title | Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology |
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| creator | Deiuliis, Jeffrey A. Li, Bing Lyvers-Peffer, Pasha A. Moeller, Steven J. Lee, Kichoon |
| description | Delta-like homolog 1 (
DLK1), a paternally imprinted gene with several alternative splicing isoforms, is an important regulator of fetal and postnatal development. We report the sequence of porcine
DLK1 (
pDLK1) and examine the expression and alternative splicing isoforms in the pig (
Sus scrofa) and human.
DLK1-A was the sole isoform identified in human tissues and has been shown to be present in mouse and cattle. Surprisingly,
DLK1-A was undetected in various tissues from fetal and postnatal pigs. Instead,
DLK1-C2 was the most abundant isoform while
DLK1-B was expressed to a lesser extent. In fractionated adipose tissue,
pDLK1 was most highly expressed in the stromal–vascular cell fraction. In addition, total
pDLK1 was highly expressed in fetal adipose tissue but dramatically decreased postnatally. Our data suggests that expression of
DLK1-B and -
C2 isoforms is sufficient for normal pig development. Furthermore, human and pig samples showed no alterations in species-specific splicing, but expression levels decreased with age, suggesting that regulation of expression, not splicing, is the most likely mechanism controlling the biological function of
DLK1. |
| doi_str_mv | 10.1016/j.cbpb.2006.06.003 |
| format | Article |
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DLK1), a paternally imprinted gene with several alternative splicing isoforms, is an important regulator of fetal and postnatal development. We report the sequence of porcine
DLK1 (
pDLK1) and examine the expression and alternative splicing isoforms in the pig (
Sus scrofa) and human.
DLK1-A was the sole isoform identified in human tissues and has been shown to be present in mouse and cattle. Surprisingly,
DLK1-A was undetected in various tissues from fetal and postnatal pigs. Instead,
DLK1-C2 was the most abundant isoform while
DLK1-B was expressed to a lesser extent. In fractionated adipose tissue,
pDLK1 was most highly expressed in the stromal–vascular cell fraction. In addition, total
pDLK1 was highly expressed in fetal adipose tissue but dramatically decreased postnatally. Our data suggests that expression of
DLK1-B and -
C2 isoforms is sufficient for normal pig development. Furthermore, human and pig samples showed no alterations in species-specific splicing, but expression levels decreased with age, suggesting that regulation of expression, not splicing, is the most likely mechanism controlling the biological function of
DLK1.</description><identifier>ISSN: 1096-4959</identifier><identifier>EISSN: 1879-1107</identifier><identifier>DOI: 10.1016/j.cbpb.2006.06.003</identifier><identifier>PMID: 16901742</identifier><language>eng</language><publisher>England: Elsevier Inc</publisher><subject>Adipocytes - metabolism ; Adipogenesis ; Adipose Tissue - cytology ; Adipose Tissue - metabolism ; Alternative Splicing ; Amino Acid Sequence ; Animals ; Base Sequence ; Callipyge ; Consensus Sequence ; DLK1 ; Humans ; Intercellular Signaling Peptides and Proteins - genetics ; Intercellular Signaling Peptides and Proteins - metabolism ; Membrane Proteins - genetics ; Membrane Proteins - metabolism ; Molecular Sequence Data ; Muscle hypertrophy ; Post-transcriptional processing ; Pref-1 ; Protein Isoforms - genetics ; Protein Isoforms - metabolism ; RNA isoforms ; RNA Splice Sites ; RNA-splicing ; Sequence Alignment ; Species Specificity ; Splice variant ; Sus scrofa ; Swine - genetics</subject><ispartof>Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 2006-09, Vol.145 (1), p.50-59</ispartof><rights>2006 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c385t-e3f6a26061d2f7c5de5fdce7db8a8416bf1815bd79f051b88fdfe9dd331eed8a3</citedby><cites>FETCH-LOGICAL-c385t-e3f6a26061d2f7c5de5fdce7db8a8416bf1815bd79f051b88fdfe9dd331eed8a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S1096495906001576$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16901742$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Deiuliis, Jeffrey A.</creatorcontrib><creatorcontrib>Li, Bing</creatorcontrib><creatorcontrib>Lyvers-Peffer, Pasha A.</creatorcontrib><creatorcontrib>Moeller, Steven J.</creatorcontrib><creatorcontrib>Lee, Kichoon</creatorcontrib><title>Alternative splicing of delta-like 1 homolog ( DLK1) in the pig and human</title><title>Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology</title><addtitle>Comp Biochem Physiol B Biochem Mol Biol</addtitle><description>Delta-like homolog 1 (
DLK1), a paternally imprinted gene with several alternative splicing isoforms, is an important regulator of fetal and postnatal development. We report the sequence of porcine
DLK1 (
pDLK1) and examine the expression and alternative splicing isoforms in the pig (
Sus scrofa) and human.
DLK1-A was the sole isoform identified in human tissues and has been shown to be present in mouse and cattle. Surprisingly,
DLK1-A was undetected in various tissues from fetal and postnatal pigs. Instead,
DLK1-C2 was the most abundant isoform while
DLK1-B was expressed to a lesser extent. In fractionated adipose tissue,
pDLK1 was most highly expressed in the stromal–vascular cell fraction. In addition, total
pDLK1 was highly expressed in fetal adipose tissue but dramatically decreased postnatally. Our data suggests that expression of
DLK1-B and -
C2 isoforms is sufficient for normal pig development. Furthermore, human and pig samples showed no alterations in species-specific splicing, but expression levels decreased with age, suggesting that regulation of expression, not splicing, is the most likely mechanism controlling the biological function of
DLK1.</description><subject>Adipocytes - metabolism</subject><subject>Adipogenesis</subject><subject>Adipose Tissue - cytology</subject><subject>Adipose Tissue - metabolism</subject><subject>Alternative Splicing</subject><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Callipyge</subject><subject>Consensus Sequence</subject><subject>DLK1</subject><subject>Humans</subject><subject>Intercellular Signaling Peptides and Proteins - genetics</subject><subject>Intercellular Signaling Peptides and Proteins - metabolism</subject><subject>Membrane Proteins - genetics</subject><subject>Membrane Proteins - metabolism</subject><subject>Molecular Sequence Data</subject><subject>Muscle hypertrophy</subject><subject>Post-transcriptional processing</subject><subject>Pref-1</subject><subject>Protein Isoforms - genetics</subject><subject>Protein Isoforms - metabolism</subject><subject>RNA isoforms</subject><subject>RNA Splice Sites</subject><subject>RNA-splicing</subject><subject>Sequence Alignment</subject><subject>Species Specificity</subject><subject>Splice variant</subject><subject>Sus scrofa</subject><subject>Swine - genetics</subject><issn>1096-4959</issn><issn>1879-1107</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtLxDAUhYMoPkb_gAvJSnTRMbeZpgm4Ed844EbXIU1uZjL2ZdMR_Pe2zIA7hQP3Lr5zFh8hp8CmwEBcraa2aItpypiYjmF8hxyCzFUCwPLd4WdKJDOVqQNyFONqACRw2CcHIBSDfJYekuebsseuNn34QhrbMthQL2jjqcOyN0kZPpACXTZVUzYLekHv5i9wSUNN-yXSNiyoqR1dritTH5M9b8qIJ9s7Ie8P92-3T8n89fH59maeWC6zPkHuhUkFE-BSn9vMYeadxdwV0sgZiMKDhKxwufIsg0JK7zwq5zgHRCcNn5DzzW7bNZ9rjL2uQrRYlqbGZh21GARACvAvmDLOlVByANMNaLsmxg69brtQme5bA9Ojab3So2k9mtZjhuaEnG3X10WF7reyVTsA1xsABxlfATsdbcDaogsd2l67Jvy1_wPGxo30</recordid><startdate>20060901</startdate><enddate>20060901</enddate><creator>Deiuliis, Jeffrey A.</creator><creator>Li, Bing</creator><creator>Lyvers-Peffer, Pasha A.</creator><creator>Moeller, Steven J.</creator><creator>Lee, Kichoon</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20060901</creationdate><title>Alternative splicing of delta-like 1 homolog ( DLK1) in the pig and human</title><author>Deiuliis, Jeffrey A. ; Li, Bing ; Lyvers-Peffer, Pasha A. ; Moeller, Steven J. ; Lee, Kichoon</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c385t-e3f6a26061d2f7c5de5fdce7db8a8416bf1815bd79f051b88fdfe9dd331eed8a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Adipocytes - metabolism</topic><topic>Adipogenesis</topic><topic>Adipose Tissue - cytology</topic><topic>Adipose Tissue - metabolism</topic><topic>Alternative Splicing</topic><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Callipyge</topic><topic>Consensus Sequence</topic><topic>DLK1</topic><topic>Humans</topic><topic>Intercellular Signaling Peptides and Proteins - genetics</topic><topic>Intercellular Signaling Peptides and Proteins - metabolism</topic><topic>Membrane Proteins - genetics</topic><topic>Membrane Proteins - metabolism</topic><topic>Molecular Sequence Data</topic><topic>Muscle hypertrophy</topic><topic>Post-transcriptional processing</topic><topic>Pref-1</topic><topic>Protein Isoforms - genetics</topic><topic>Protein Isoforms - metabolism</topic><topic>RNA isoforms</topic><topic>RNA Splice Sites</topic><topic>RNA-splicing</topic><topic>Sequence Alignment</topic><topic>Species Specificity</topic><topic>Splice variant</topic><topic>Sus scrofa</topic><topic>Swine - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Deiuliis, Jeffrey A.</creatorcontrib><creatorcontrib>Li, Bing</creatorcontrib><creatorcontrib>Lyvers-Peffer, Pasha A.</creatorcontrib><creatorcontrib>Moeller, Steven J.</creatorcontrib><creatorcontrib>Lee, Kichoon</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Deiuliis, Jeffrey A.</au><au>Li, Bing</au><au>Lyvers-Peffer, Pasha A.</au><au>Moeller, Steven J.</au><au>Lee, Kichoon</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Alternative splicing of delta-like 1 homolog ( DLK1) in the pig and human</atitle><jtitle>Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology</jtitle><addtitle>Comp Biochem Physiol B Biochem Mol Biol</addtitle><date>2006-09-01</date><risdate>2006</risdate><volume>145</volume><issue>1</issue><spage>50</spage><epage>59</epage><pages>50-59</pages><issn>1096-4959</issn><eissn>1879-1107</eissn><abstract>Delta-like homolog 1 (
DLK1), a paternally imprinted gene with several alternative splicing isoforms, is an important regulator of fetal and postnatal development. We report the sequence of porcine
DLK1 (
pDLK1) and examine the expression and alternative splicing isoforms in the pig (
Sus scrofa) and human.
DLK1-A was the sole isoform identified in human tissues and has been shown to be present in mouse and cattle. Surprisingly,
DLK1-A was undetected in various tissues from fetal and postnatal pigs. Instead,
DLK1-C2 was the most abundant isoform while
DLK1-B was expressed to a lesser extent. In fractionated adipose tissue,
pDLK1 was most highly expressed in the stromal–vascular cell fraction. In addition, total
pDLK1 was highly expressed in fetal adipose tissue but dramatically decreased postnatally. Our data suggests that expression of
DLK1-B and -
C2 isoforms is sufficient for normal pig development. Furthermore, human and pig samples showed no alterations in species-specific splicing, but expression levels decreased with age, suggesting that regulation of expression, not splicing, is the most likely mechanism controlling the biological function of
DLK1.</abstract><cop>England</cop><pub>Elsevier Inc</pub><pmid>16901742</pmid><doi>10.1016/j.cbpb.2006.06.003</doi><tpages>10</tpages></addata></record> |
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| ispartof | Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 2006-09, Vol.145 (1), p.50-59 |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Adipocytes - metabolism Adipogenesis Adipose Tissue - cytology Adipose Tissue - metabolism Alternative Splicing Amino Acid Sequence Animals Base Sequence Callipyge Consensus Sequence DLK1 Humans Intercellular Signaling Peptides and Proteins - genetics Intercellular Signaling Peptides and Proteins - metabolism Membrane Proteins - genetics Membrane Proteins - metabolism Molecular Sequence Data Muscle hypertrophy Post-transcriptional processing Pref-1 Protein Isoforms - genetics Protein Isoforms - metabolism RNA isoforms RNA Splice Sites RNA-splicing Sequence Alignment Species Specificity Splice variant Sus scrofa Swine - genetics |
| title | Alternative splicing of delta-like 1 homolog ( DLK1) in the pig and human |
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