Identification of eel ghrelin in plasma and stomach by radioimmunoassay and histochemistry

The structure of ghrelin has been determined in the Japanese eel, Anguilla japonica. In this study, we identified immunoreactive ghrelin in extracts from plasma and stomach of the eel by radioimmunoassay (RIA) using an antiserum against octanoylated rat ghrelin [1–11]. Using the antiserum, we examin...

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Veröffentlicht in:	General and comparative endocrinology 2006-09, Vol.148 (3), p.375-382
Hauptverfasser:	Kaiya, Hiroyuki, Tsukada, Takehiro, Yuge, Shinya, Mondo, Hiroko, Kangawa, Kenji, Takei, Yoshio
Format:	Artikel
Sprache:	eng
Schlagworte:	Acclimatization - physiology Anguilla japonica Animals Brackish Eel Eels - anatomy & histology Eels - blood Eels - metabolism Female Fresh Water Freshwater Ghrelin Histocytochemistry - methods Immunohistochemistry In situ hybridization Male Marine Osmolar Concentration Peptide Hormones - metabolism Polymerase Chain Reaction Radioimmunoassay - methods RIA RNA, Complementary - metabolism Seawater - adverse effects Stomach - metabolism
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