N-Terminal protein modifications in an insect cell-free protein synthesis system and their identification by mass spectrometry

To evaluate the ability of an insect cell‐free protein synthesis system to generate proper N‐terminal cotranslational protein modifications such as removal of the initiating Met, N‐acetylation, and N‐myristoylation, several mutants were constructed using truncated human gelsolin (tGelsolin) as a mod...

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