Evaluation of vascular endothelial growth factor addition in vitro on mouse inner ear progenitor cell cultures
We analyzed progenitor cell cultures of inner ear tissue from newborn mice, and found proliferating cells, morphologically differentiating cells and subpopulations of cells expressing either neuronal or glial markers. In addition, we observed the expression of fetal liver kinase-1, a receptor for th...
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| Veröffentlicht in: | Neuroreport 2006-09, Vol.17 (13), p.1369-1373 |
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| creator | Fraenzer, Juergen-Theodor Wachs, Frank-Peter Gleich, Otto Licht, Anna-Katharina Strutz, Jürgen |
| description | We analyzed progenitor cell cultures of inner ear tissue from newborn mice, and found proliferating cells, morphologically differentiating cells and subpopulations of cells expressing either neuronal or glial markers. In addition, we observed the expression of fetal liver kinase-1, a receptor for the vascular endothelial growth factor in a subpopulation of the cultured cells. Consistent with the expression of fetal liver kinase-1, addition of vascular endothelial growth factor at a dose of 10 ng/ml increased the expansion rate of inner ear-derived progenitor cells. Together with other published data, these results suggest that the vascular endothelial growth factor might be involved in inducing or supporting cochlear repair processes. |
| doi_str_mv | 10.1097/01.wnr.0000233101.05408.ad |
| format | Article |
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In addition, we observed the expression of fetal liver kinase-1, a receptor for the vascular endothelial growth factor in a subpopulation of the cultured cells. Consistent with the expression of fetal liver kinase-1, addition of vascular endothelial growth factor at a dose of 10 ng/ml increased the expansion rate of inner ear-derived progenitor cells. Together with other published data, these results suggest that the vascular endothelial growth factor might be involved in inducing or supporting cochlear repair processes.</description><identifier>ISSN: 0959-4965</identifier><identifier>EISSN: 1473-558X</identifier><identifier>DOI: 10.1097/01.wnr.0000233101.05408.ad</identifier><identifier>PMID: 16932141</identifier><language>eng</language><publisher>Hagerstown, MD: Lippincott Williams & Wilkins, Inc</publisher><subject>Alanine - analogs & derivatives ; Alanine - pharmacology ; Animals ; Animals, Newborn ; Biological and medical sciences ; Cell Count - methods ; Cell Differentiation - drug effects ; Cell Proliferation - drug effects ; Cells, Cultured ; Dose-Response Relationship, Drug ; Ear and associated structures. Auditory pathways and centers. Hearing. Vocal organ. Phonation. Sound production. Echolocation ; Ear, Inner - cytology ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation, Developmental - drug effects ; Gene Expression Regulation, Developmental - physiology ; Immunohistochemistry - methods ; Mice ; Reverse Transcriptase Polymerase Chain Reaction - methods ; RNA, Messenger - biosynthesis ; Stem Cells - drug effects ; Stem Cells - enzymology ; Vascular Endothelial Growth Factor A - pharmacology ; Vascular Endothelial Growth Factor Receptor-2 - metabolism ; Vertebrates: nervous system and sense organs</subject><ispartof>Neuroreport, 2006-09, Vol.17 (13), p.1369-1373</ispartof><rights>2006 Lippincott Williams & Wilkins, Inc.</rights><rights>2006 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4231-d841c7f66f9385e80890e3e0c65743e2da7435d3b00bf7577047b824aabcde343</citedby><cites>FETCH-LOGICAL-c4231-d841c7f66f9385e80890e3e0c65743e2da7435d3b00bf7577047b824aabcde343</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18083008$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16932141$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fraenzer, Juergen-Theodor</creatorcontrib><creatorcontrib>Wachs, Frank-Peter</creatorcontrib><creatorcontrib>Gleich, Otto</creatorcontrib><creatorcontrib>Licht, Anna-Katharina</creatorcontrib><creatorcontrib>Strutz, Jürgen</creatorcontrib><title>Evaluation of vascular endothelial growth factor addition in vitro on mouse inner ear progenitor cell cultures</title><title>Neuroreport</title><addtitle>Neuroreport</addtitle><description>We analyzed progenitor cell cultures of inner ear tissue from newborn mice, and found proliferating cells, morphologically differentiating cells and subpopulations of cells expressing either neuronal or glial markers. 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Together with other published data, these results suggest that the vascular endothelial growth factor might be involved in inducing or supporting cochlear repair processes.</description><subject>Alanine - analogs & derivatives</subject><subject>Alanine - pharmacology</subject><subject>Animals</subject><subject>Animals, Newborn</subject><subject>Biological and medical sciences</subject><subject>Cell Count - methods</subject><subject>Cell Differentiation - drug effects</subject><subject>Cell Proliferation - drug effects</subject><subject>Cells, Cultured</subject><subject>Dose-Response Relationship, Drug</subject><subject>Ear and associated structures. Auditory pathways and centers. Hearing. Vocal organ. Phonation. Sound production. Echolocation</subject><subject>Ear, Inner - cytology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation, Developmental - drug effects</subject><subject>Gene Expression Regulation, Developmental - physiology</subject><subject>Immunohistochemistry - methods</subject><subject>Mice</subject><subject>Reverse Transcriptase Polymerase Chain Reaction - methods</subject><subject>RNA, Messenger - biosynthesis</subject><subject>Stem Cells - drug effects</subject><subject>Stem Cells - enzymology</subject><subject>Vascular Endothelial Growth Factor A - pharmacology</subject><subject>Vascular Endothelial Growth Factor Receptor-2 - metabolism</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0959-4965</issn><issn>1473-558X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU2LFDEQhoMo7jj6F6QR9NZjpZN00t5kWT9gwYuCt5DuVO-0Zjprkp7Bf2_NB8zRQKhK8bxVRV7G3nDYcOj0e-Cbw5w2QKcRgtMTlASzcf4JW3GpRa2U-fmUraBTXS27Vt2wFzn_Ir4Dbp6zG952ouGSr9h8t3dhcWWKcxXHau_ysASXKpx9LFsMkwvVQ4qHsq1GN5SYKuf9dMKnudpPJcWK8l1cMlJlRpKS_DHFB5ynIz9gCBU1LUvC_JI9G13I-OoS1-zHp7vvt1_q-2-fv95-vK8H2QheeyP5oMe2HTthFBowHaBAGFqlpcDGOwrKix6gH7XSGqTuTSOd6wePQoo1e3fuS4v8WTAXu5vycRM3I61qW6NNy6X6L9iAMsoYTuCHMzikmHPC0T6maefSX8vBHm2xwC3ZYq-22JMt1nkSv75MWfod-qv04gMBby8AGeDCmNw8TPnK0Q8IoLtm8swdYiiY8u-wHDDZLbpQtqfRXKu2bgBa6EhVn0riHzIiqGM</recordid><startdate>20060918</startdate><enddate>20060918</enddate><creator>Fraenzer, Juergen-Theodor</creator><creator>Wachs, Frank-Peter</creator><creator>Gleich, Otto</creator><creator>Licht, Anna-Katharina</creator><creator>Strutz, Jürgen</creator><general>Lippincott Williams & Wilkins, Inc</general><general>Lippincott Williams and Wilkins</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7TK</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20060918</creationdate><title>Evaluation of vascular endothelial growth factor addition in vitro on mouse inner ear progenitor cell cultures</title><author>Fraenzer, Juergen-Theodor ; Wachs, Frank-Peter ; Gleich, Otto ; Licht, Anna-Katharina ; Strutz, Jürgen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4231-d841c7f66f9385e80890e3e0c65743e2da7435d3b00bf7577047b824aabcde343</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Alanine - analogs & derivatives</topic><topic>Alanine - pharmacology</topic><topic>Animals</topic><topic>Animals, Newborn</topic><topic>Biological and medical sciences</topic><topic>Cell Count - methods</topic><topic>Cell Differentiation - drug effects</topic><topic>Cell Proliferation - drug effects</topic><topic>Cells, Cultured</topic><topic>Dose-Response Relationship, Drug</topic><topic>Ear and associated structures. Auditory pathways and centers. Hearing. Vocal organ. Phonation. Sound production. Echolocation</topic><topic>Ear, Inner - cytology</topic><topic>Fundamental and applied biological sciences. 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In addition, we observed the expression of fetal liver kinase-1, a receptor for the vascular endothelial growth factor in a subpopulation of the cultured cells. Consistent with the expression of fetal liver kinase-1, addition of vascular endothelial growth factor at a dose of 10 ng/ml increased the expansion rate of inner ear-derived progenitor cells. Together with other published data, these results suggest that the vascular endothelial growth factor might be involved in inducing or supporting cochlear repair processes.</abstract><cop>Hagerstown, MD</cop><pub>Lippincott Williams & Wilkins, Inc</pub><pmid>16932141</pmid><doi>10.1097/01.wnr.0000233101.05408.ad</doi><tpages>5</tpages></addata></record> |
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| subjects | Alanine - analogs & derivatives Alanine - pharmacology Animals Animals, Newborn Biological and medical sciences Cell Count - methods Cell Differentiation - drug effects Cell Proliferation - drug effects Cells, Cultured Dose-Response Relationship, Drug Ear and associated structures. Auditory pathways and centers. Hearing. Vocal organ. Phonation. Sound production. Echolocation Ear, Inner - cytology Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Developmental - drug effects Gene Expression Regulation, Developmental - physiology Immunohistochemistry - methods Mice Reverse Transcriptase Polymerase Chain Reaction - methods RNA, Messenger - biosynthesis Stem Cells - drug effects Stem Cells - enzymology Vascular Endothelial Growth Factor A - pharmacology Vascular Endothelial Growth Factor Receptor-2 - metabolism Vertebrates: nervous system and sense organs |
| title | Evaluation of vascular endothelial growth factor addition in vitro on mouse inner ear progenitor cell cultures |
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