2-D differential membrane proteome analysis of scarce protein samples

Proteome studies with small sample amounts are difficult to perform, especially when membrane proteins are the focus of interest. In our study a new method for the analysis of scarce membrane protein samples combining large gel 2‐D‐CTAB/SDS‐PAGE with fluorescence dye saturation labelling (satDIGE) w...

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Veröffentlicht in:	Proteomics (Weinheim) 2006-08, Vol.6 (16), p.4506-4513
Hauptverfasser:	Helling, Stefan, Schmitt, Edgar, Joppich, Cornelia, Schulenborg, Thomas, Müllner, Stefan, Felske-Müller, Stephanie, Wiebringhaus, Thomas, Becker, Gabriele, Linsenmann, Gudrun, Sitek, Barbara, Lutter, Petra, Meyer, Helmut E., Marcus, Katrin
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Analytical, structural and metabolic biochemistry Animals Biological and medical sciences Cells, Cultured Electrophoresis, Gel, Two-Dimensional Fluorescence two-dimensional difference gel electrophoresis Fluorescent Dyes - chemistry Fundamental and applied biological sciences. Psychology Humans Membrane proteins Membrane Proteins - analysis Membrane Proteins - isolation & purification Mice Miscellaneous Molecular Sequence Data Multiplexed proteomics technology Proteins Proteome Proteomics methods satDIGE Spectrometry, Mass, Electrospray Ionization
Online-Zugang:	Volltext
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