Use of a novel serum ELISA method and the tonsil-carrier state for evaluation of Mycoplasma hyosynoviae distributions in pig herds with or without clinical arthritis
A novel indirect ELISA test using deoxycholate extracted antigens coated onto a hydrophobic polystyrene surface for measurement of serum antibodies specific for Mycoplasma hyosynoviae was developed. Sensitivity and specificity of the test were found to be superior to previous ELISAs as tested on por...
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| Veröffentlicht in: | Veterinary microbiology 2005-11, Vol.111 (1-2), p.41-50 |
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| creator | Nielsen, Elisabeth Okholm Lauritsen, Klara Tølbøll Friis, Niels Filskov Enøe, Claes Hagedorn-Olsen, Tine Jungersen, Gregers |
| description | A novel indirect ELISA test using deoxycholate extracted antigens coated onto a hydrophobic polystyrene surface for measurement of serum antibodies specific for Mycoplasma hyosynoviae was developed. Sensitivity and specificity of the test were found to be superior to previous ELISAs as tested on porcine serum following experimental Mycoplasma infections as well as by analysis of samples from one Danish herd known to be free of M. hyosynoviae and samples from two Norwegian herds without clinical suspicion of M. hyosynoviae infections since their establishment. The epidemiology of M. hyosynoviae infection was then investigated in Danish pig herds with evidence of clinical M. hyosynoviae arthritis (MhA herds, n=4) and in herds with M. hyosynoviae-carriers among slaughter pigs, but with limited clinical lameness (MhC herds, n=4). M. hyosynoviae bacteriaemia and tonsil-carrier state were determined by culture of cross-sectional samples of whole-blood (n=238) and tonsil scrapings (n=322), respectively. Levels of serum antibodies (n=396) were measured by the novel indirect ELISA test.
There was no significant difference in the ELISA results between the MhA and the MhC herds. Pigs that were tonsil-carriers had a significantly higher response in the ELISA test (P |
| doi_str_mv | 10.1016/j.vetmic.2005.08.009 |
| format | Article |
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There was no significant difference in the ELISA results between the MhA and the MhC herds. Pigs that were tonsil-carriers had a significantly higher response in the ELISA test (P<0.001) than non-carriers. Slaughter pigs showed higher ELISA values (P<0.001) and they were more prone to be tonsil-carriers (P<0.001). The most critical period for spread of the infection seems to be the nursery period (4–12 weeks).
The results indicate that M. hyosynoviae infection progresses similarly in herds irrespective of the presence of clinical arthritis. Thus, clinical arthritis due to M. hyosynoviae is probably triggered by other host or herd factors than low levels of serum antibodies or by differences in virulence factors between M. hyosynoviae strains.</description><identifier>ISSN: 0378-1135</identifier><identifier>EISSN: 1873-2542</identifier><identifier>DOI: 10.1016/j.vetmic.2005.08.009</identifier><identifier>PMID: 16171955</identifier><identifier>CODEN: VMICDQ</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Animals ; Animals, Newborn ; Antibodies, Bacterial - blood ; Arthritis ; Arthritis, Infectious - diagnosis ; Arthritis, Infectious - epidemiology ; Arthritis, Infectious - veterinary ; Bacteriology ; Biological and medical sciences ; Carrier State - diagnosis ; Carrier State - epidemiology ; Carrier State - veterinary ; Cross-Sectional Studies ; Denmark - epidemiology ; ELISA ; Enzyme-Linked Immunosorbent Assay - methods ; Enzyme-Linked Immunosorbent Assay - veterinary ; Fundamental and applied biological sciences. Psychology ; M. hyosynoviae ; Microbiology ; Miscellaneous ; Mycoplasma hyosynoviae ; Mycoplasma hyosynoviae - immunology ; Mycoplasma Infections - diagnosis ; Mycoplasma Infections - epidemiology ; Mycoplasma Infections - veterinary ; Norway - epidemiology ; Palatine Tonsil - microbiology ; Pigs ; Sensitivity and Specificity ; Seroepidemiologic Studies ; Swine ; Swine Diseases - diagnosis ; Swine Diseases - epidemiology ; Tonsil-carrier state ; Virulence Factors</subject><ispartof>Veterinary microbiology, 2005-11, Vol.111 (1-2), p.41-50</ispartof><rights>2005 Elsevier B.V.</rights><rights>2006 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c421t-6305ca862fc2f180eb898c78d24a1de7c2cef5755084a86b7759ed54ebbb67773</citedby><cites>FETCH-LOGICAL-c421t-6305ca862fc2f180eb898c78d24a1de7c2cef5755084a86b7759ed54ebbb67773</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0378113505002713$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17258520$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16171955$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nielsen, Elisabeth Okholm</creatorcontrib><creatorcontrib>Lauritsen, Klara Tølbøll</creatorcontrib><creatorcontrib>Friis, Niels Filskov</creatorcontrib><creatorcontrib>Enøe, Claes</creatorcontrib><creatorcontrib>Hagedorn-Olsen, Tine</creatorcontrib><creatorcontrib>Jungersen, Gregers</creatorcontrib><title>Use of a novel serum ELISA method and the tonsil-carrier state for evaluation of Mycoplasma hyosynoviae distributions in pig herds with or without clinical arthritis</title><title>Veterinary microbiology</title><addtitle>Vet Microbiol</addtitle><description>A novel indirect ELISA test using deoxycholate extracted antigens coated onto a hydrophobic polystyrene surface for measurement of serum antibodies specific for Mycoplasma hyosynoviae was developed. Sensitivity and specificity of the test were found to be superior to previous ELISAs as tested on porcine serum following experimental Mycoplasma infections as well as by analysis of samples from one Danish herd known to be free of M. hyosynoviae and samples from two Norwegian herds without clinical suspicion of M. hyosynoviae infections since their establishment. The epidemiology of M. hyosynoviae infection was then investigated in Danish pig herds with evidence of clinical M. hyosynoviae arthritis (MhA herds, n=4) and in herds with M. hyosynoviae-carriers among slaughter pigs, but with limited clinical lameness (MhC herds, n=4). M. hyosynoviae bacteriaemia and tonsil-carrier state were determined by culture of cross-sectional samples of whole-blood (n=238) and tonsil scrapings (n=322), respectively. Levels of serum antibodies (n=396) were measured by the novel indirect ELISA test.
There was no significant difference in the ELISA results between the MhA and the MhC herds. Pigs that were tonsil-carriers had a significantly higher response in the ELISA test (P<0.001) than non-carriers. Slaughter pigs showed higher ELISA values (P<0.001) and they were more prone to be tonsil-carriers (P<0.001). The most critical period for spread of the infection seems to be the nursery period (4–12 weeks).
The results indicate that M. hyosynoviae infection progresses similarly in herds irrespective of the presence of clinical arthritis. Thus, clinical arthritis due to M. hyosynoviae is probably triggered by other host or herd factors than low levels of serum antibodies or by differences in virulence factors between M. hyosynoviae strains.</description><subject>Animals</subject><subject>Animals, Newborn</subject><subject>Antibodies, Bacterial - blood</subject><subject>Arthritis</subject><subject>Arthritis, Infectious - diagnosis</subject><subject>Arthritis, Infectious - epidemiology</subject><subject>Arthritis, Infectious - veterinary</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Carrier State - diagnosis</subject><subject>Carrier State - epidemiology</subject><subject>Carrier State - veterinary</subject><subject>Cross-Sectional Studies</subject><subject>Denmark - epidemiology</subject><subject>ELISA</subject><subject>Enzyme-Linked Immunosorbent Assay - methods</subject><subject>Enzyme-Linked Immunosorbent Assay - veterinary</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>M. hyosynoviae</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>Mycoplasma hyosynoviae</subject><subject>Mycoplasma hyosynoviae - immunology</subject><subject>Mycoplasma Infections - diagnosis</subject><subject>Mycoplasma Infections - epidemiology</subject><subject>Mycoplasma Infections - veterinary</subject><subject>Norway - epidemiology</subject><subject>Palatine Tonsil - microbiology</subject><subject>Pigs</subject><subject>Sensitivity and Specificity</subject><subject>Seroepidemiologic Studies</subject><subject>Swine</subject><subject>Swine Diseases - diagnosis</subject><subject>Swine Diseases - epidemiology</subject><subject>Tonsil-carrier state</subject><subject>Virulence Factors</subject><issn>0378-1135</issn><issn>1873-2542</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcGO0zAQQCMEYsvCHyA0F7il2G4cOxek1WqBlYo4wJ4tx5kQV0lcPE5X_SD-E1ettDc4zeXN02heUbzlbM0Zrz_u1gdMk3drwZhcM71mrHlWrLhWm1LISjwvVmyjdMn5Rl4Vr4h2jLGqqdnL4orXXPFGylXx54EQQg8W5nDAEQjjMsHd9v7HDUyYhtCBnTtIA0IKM_mxdDZGjxEo2YTQhwh4sONikw_zyfTt6MJ-tDRZGI6BjtnrLULnKUXfLieMwM-w979gwNgRPPo0QPacZlgSuNHP3tkRbExD9MnT6-JFb0fCN5d5XTx8vvt5-7Xcfv9yf3uzLV0leCrrDZPO6lr0TvRcM2x1o53Snags71A54bCXSkqmq4y1SskGO1lh27a1UmpzXXw4e_cx_F6Qkpk8ORxHO2NYyNRaaVGJ5r8gb4Ru6kpnsDqDLgaiiL3ZRz_ZeDScmVNHszPnjubU0TBtcse89u7iX9oJu6elS7gMvL8AlvKr-mhn5-mJU0JqKVjmPp05zG875G6GnMfZYecjumS64P99yV8N0cEb</recordid><startdate>20051130</startdate><enddate>20051130</enddate><creator>Nielsen, Elisabeth Okholm</creator><creator>Lauritsen, Klara Tølbøll</creator><creator>Friis, Niels Filskov</creator><creator>Enøe, Claes</creator><creator>Hagedorn-Olsen, Tine</creator><creator>Jungersen, Gregers</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20051130</creationdate><title>Use of a novel serum ELISA method and the tonsil-carrier state for evaluation of Mycoplasma hyosynoviae distributions in pig herds with or without clinical arthritis</title><author>Nielsen, Elisabeth Okholm ; Lauritsen, Klara Tølbøll ; Friis, Niels Filskov ; Enøe, Claes ; Hagedorn-Olsen, Tine ; Jungersen, Gregers</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c421t-6305ca862fc2f180eb898c78d24a1de7c2cef5755084a86b7759ed54ebbb67773</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Animals</topic><topic>Animals, Newborn</topic><topic>Antibodies, Bacterial - blood</topic><topic>Arthritis</topic><topic>Arthritis, Infectious - diagnosis</topic><topic>Arthritis, Infectious - epidemiology</topic><topic>Arthritis, Infectious - veterinary</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Carrier State - diagnosis</topic><topic>Carrier State - epidemiology</topic><topic>Carrier State - veterinary</topic><topic>Cross-Sectional Studies</topic><topic>Denmark - epidemiology</topic><topic>ELISA</topic><topic>Enzyme-Linked Immunosorbent Assay - methods</topic><topic>Enzyme-Linked Immunosorbent Assay - veterinary</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>M. hyosynoviae</topic><topic>Microbiology</topic><topic>Miscellaneous</topic><topic>Mycoplasma hyosynoviae</topic><topic>Mycoplasma hyosynoviae - immunology</topic><topic>Mycoplasma Infections - diagnosis</topic><topic>Mycoplasma Infections - epidemiology</topic><topic>Mycoplasma Infections - veterinary</topic><topic>Norway - epidemiology</topic><topic>Palatine Tonsil - microbiology</topic><topic>Pigs</topic><topic>Sensitivity and Specificity</topic><topic>Seroepidemiologic Studies</topic><topic>Swine</topic><topic>Swine Diseases - diagnosis</topic><topic>Swine Diseases - epidemiology</topic><topic>Tonsil-carrier state</topic><topic>Virulence Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nielsen, Elisabeth Okholm</creatorcontrib><creatorcontrib>Lauritsen, Klara Tølbøll</creatorcontrib><creatorcontrib>Friis, Niels Filskov</creatorcontrib><creatorcontrib>Enøe, Claes</creatorcontrib><creatorcontrib>Hagedorn-Olsen, Tine</creatorcontrib><creatorcontrib>Jungersen, Gregers</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Veterinary microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nielsen, Elisabeth Okholm</au><au>Lauritsen, Klara Tølbøll</au><au>Friis, Niels Filskov</au><au>Enøe, Claes</au><au>Hagedorn-Olsen, Tine</au><au>Jungersen, Gregers</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Use of a novel serum ELISA method and the tonsil-carrier state for evaluation of Mycoplasma hyosynoviae distributions in pig herds with or without clinical arthritis</atitle><jtitle>Veterinary microbiology</jtitle><addtitle>Vet Microbiol</addtitle><date>2005-11-30</date><risdate>2005</risdate><volume>111</volume><issue>1-2</issue><spage>41</spage><epage>50</epage><pages>41-50</pages><issn>0378-1135</issn><eissn>1873-2542</eissn><coden>VMICDQ</coden><abstract>A novel indirect ELISA test using deoxycholate extracted antigens coated onto a hydrophobic polystyrene surface for measurement of serum antibodies specific for Mycoplasma hyosynoviae was developed. Sensitivity and specificity of the test were found to be superior to previous ELISAs as tested on porcine serum following experimental Mycoplasma infections as well as by analysis of samples from one Danish herd known to be free of M. hyosynoviae and samples from two Norwegian herds without clinical suspicion of M. hyosynoviae infections since their establishment. The epidemiology of M. hyosynoviae infection was then investigated in Danish pig herds with evidence of clinical M. hyosynoviae arthritis (MhA herds, n=4) and in herds with M. hyosynoviae-carriers among slaughter pigs, but with limited clinical lameness (MhC herds, n=4). M. hyosynoviae bacteriaemia and tonsil-carrier state were determined by culture of cross-sectional samples of whole-blood (n=238) and tonsil scrapings (n=322), respectively. Levels of serum antibodies (n=396) were measured by the novel indirect ELISA test.
There was no significant difference in the ELISA results between the MhA and the MhC herds. Pigs that were tonsil-carriers had a significantly higher response in the ELISA test (P<0.001) than non-carriers. Slaughter pigs showed higher ELISA values (P<0.001) and they were more prone to be tonsil-carriers (P<0.001). The most critical period for spread of the infection seems to be the nursery period (4–12 weeks).
The results indicate that M. hyosynoviae infection progresses similarly in herds irrespective of the presence of clinical arthritis. Thus, clinical arthritis due to M. hyosynoviae is probably triggered by other host or herd factors than low levels of serum antibodies or by differences in virulence factors between M. hyosynoviae strains.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>16171955</pmid><doi>10.1016/j.vetmic.2005.08.009</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Veterinary microbiology, 2005-11, Vol.111 (1-2), p.41-50 |
| issn | 0378-1135 1873-2542 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Animals, Newborn Antibodies, Bacterial - blood Arthritis Arthritis, Infectious - diagnosis Arthritis, Infectious - epidemiology Arthritis, Infectious - veterinary Bacteriology Biological and medical sciences Carrier State - diagnosis Carrier State - epidemiology Carrier State - veterinary Cross-Sectional Studies Denmark - epidemiology ELISA Enzyme-Linked Immunosorbent Assay - methods Enzyme-Linked Immunosorbent Assay - veterinary Fundamental and applied biological sciences. Psychology M. hyosynoviae Microbiology Miscellaneous Mycoplasma hyosynoviae Mycoplasma hyosynoviae - immunology Mycoplasma Infections - diagnosis Mycoplasma Infections - epidemiology Mycoplasma Infections - veterinary Norway - epidemiology Palatine Tonsil - microbiology Pigs Sensitivity and Specificity Seroepidemiologic Studies Swine Swine Diseases - diagnosis Swine Diseases - epidemiology Tonsil-carrier state Virulence Factors |
| title | Use of a novel serum ELISA method and the tonsil-carrier state for evaluation of Mycoplasma hyosynoviae distributions in pig herds with or without clinical arthritis |
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