Dual regulatory effect of plant extracts of Forsythia suspense on RANTES and MCP-1 secretion in influenza A virus-infected human bronchial epithelial cells
In this study, we investigated the effects of 95% ethanol (FS-t1), 50% ethanol (FS-t2) and water (FS-w) extracts of Forsythia suspense Vahl (Oleaceae) on the production of regulated on activation, normal T cell expressed and secreted (RANTES) and macrophage chemotactic protein-1 (MCP-1) by influenza...
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| Veröffentlicht in: | Journal of ethnopharmacology 2005-12, Vol.102 (3), p.418-423 |
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| creator | Ko, Han-Chieh Wei, Bai-Luh Chiou, Wen-Fei |
| description | In this study, we investigated the effects of 95% ethanol (FS-t1), 50% ethanol (FS-t2) and water (FS-w) extracts of
Forsythia suspense Vahl (Oleaceae) on the production of regulated on activation, normal T cell expressed and secreted (RANTES) and macrophage chemotactic protein-1 (MCP-1) by influenza A virus (H1N1)-infected human bronchial epithelial cell line A549. Virus infection evoked a markedly enhanced production of RANTES from basal 16
±
4 to 1307
±
294
pg/ml after 72
h inoculation. At the non-cytotoxic doses (20, 100 and 200
μg/ml), FS-t1, FS-t2 and FS-w exhibited a consistent inhibitory effect on virus-stimulated RANTES secretion in a dose-dependent manner wilh IC
50 of 42
±
6, 117
±
15 and 232
±
28
μg/ml, respectively. H1N1 also stimulated MCP-1 production in A549 cells, however to a less degree, from basal 133
±
21 to 391
±
98
pg/ml after 72
h viral inoculation. The effects of three extracts on MCP-1 secretion were more complex. FS-t1 displayed both positive and negative effect on virus-stimulated MCP-1 production dependent on the concentrations used. On the other hand, FS-t2 increased virus-induced MCP-1 secretion by 1.4–3.3 times while the third fraction FO-w increased by 2.6–3.7 times. These results suggested that
Forsythia suspense consisted of both negative and positive regulatory components on RANTES and MCP-1 secretion in H1N1-infected A549 cells, respectively. |
| doi_str_mv | 10.1016/j.jep.2005.06.029 |
| format | Article |
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Forsythia suspense Vahl (Oleaceae) on the production of regulated on activation, normal T cell expressed and secreted (RANTES) and macrophage chemotactic protein-1 (MCP-1) by influenza A virus (H1N1)-infected human bronchial epithelial cell line A549. Virus infection evoked a markedly enhanced production of RANTES from basal 16
±
4 to 1307
±
294
pg/ml after 72
h inoculation. At the non-cytotoxic doses (20, 100 and 200
μg/ml), FS-t1, FS-t2 and FS-w exhibited a consistent inhibitory effect on virus-stimulated RANTES secretion in a dose-dependent manner wilh IC
50 of 42
±
6, 117
±
15 and 232
±
28
μg/ml, respectively. H1N1 also stimulated MCP-1 production in A549 cells, however to a less degree, from basal 133
±
21 to 391
±
98
pg/ml after 72
h viral inoculation. The effects of three extracts on MCP-1 secretion were more complex. FS-t1 displayed both positive and negative effect on virus-stimulated MCP-1 production dependent on the concentrations used. On the other hand, FS-t2 increased virus-induced MCP-1 secretion by 1.4–3.3 times while the third fraction FO-w increased by 2.6–3.7 times. These results suggested that
Forsythia suspense consisted of both negative and positive regulatory components on RANTES and MCP-1 secretion in H1N1-infected A549 cells, respectively.</description><identifier>ISSN: 0378-8741</identifier><identifier>EISSN: 1872-7573</identifier><identifier>DOI: 10.1016/j.jep.2005.06.029</identifier><identifier>PMID: 16054313</identifier><identifier>CODEN: JOETD7</identifier><language>eng</language><publisher>Shannon: Elsevier Ireland Ltd</publisher><subject>Animals ; antiviral agents ; Biological and medical sciences ; bronchi ; Bronchi - metabolism ; Bronchi - virology ; Cells, Cultured ; chemical constituents of plants ; Chemokine CCL2 - biosynthesis ; Chemokine CCL5 - biosynthesis ; chemotaxis ; Dogs ; epithelial cells ; Epithelial Cells - metabolism ; Epithelial Cells - virology ; ethnobotany ; Forsythia ; Forsythia suspensa ; Forsythia suspense Vahl (Oleaceae) ; fruits (plant anatomy) ; General pharmacology ; herbal medicines ; Humans ; Influenza A virus ; Influenza A Virus, H1N1 Subtype - pathogenicity ; Macrophage chemotactic protein-1 ; macrophages ; Medical sciences ; medicinal plants ; medicinal properties ; Normal T cell expressed and secreted ; Oriental traditional medicine ; Pharmacognosy. Homeopathy. Health food ; Pharmacology. Drug treatments ; plant extracts ; Plant Extracts - pharmacology ; protein secretion ; Regulated on activation</subject><ispartof>Journal of ethnopharmacology, 2005-12, Vol.102 (3), p.418-423</ispartof><rights>2005 Elsevier Ireland Ltd</rights><rights>2006 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c405t-d98cb5ea602a1bafad80446f545e76b3089af518c950f4f6fa63005b5a4cf07d3</citedby><cites>FETCH-LOGICAL-c405t-d98cb5ea602a1bafad80446f545e76b3089af518c950f4f6fa63005b5a4cf07d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jep.2005.06.029$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27923,27924,45994</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17270760$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16054313$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ko, Han-Chieh</creatorcontrib><creatorcontrib>Wei, Bai-Luh</creatorcontrib><creatorcontrib>Chiou, Wen-Fei</creatorcontrib><title>Dual regulatory effect of plant extracts of Forsythia suspense on RANTES and MCP-1 secretion in influenza A virus-infected human bronchial epithelial cells</title><title>Journal of ethnopharmacology</title><addtitle>J Ethnopharmacol</addtitle><description>In this study, we investigated the effects of 95% ethanol (FS-t1), 50% ethanol (FS-t2) and water (FS-w) extracts of
Forsythia suspense Vahl (Oleaceae) on the production of regulated on activation, normal T cell expressed and secreted (RANTES) and macrophage chemotactic protein-1 (MCP-1) by influenza A virus (H1N1)-infected human bronchial epithelial cell line A549. Virus infection evoked a markedly enhanced production of RANTES from basal 16
±
4 to 1307
±
294
pg/ml after 72
h inoculation. At the non-cytotoxic doses (20, 100 and 200
μg/ml), FS-t1, FS-t2 and FS-w exhibited a consistent inhibitory effect on virus-stimulated RANTES secretion in a dose-dependent manner wilh IC
50 of 42
±
6, 117
±
15 and 232
±
28
μg/ml, respectively. H1N1 also stimulated MCP-1 production in A549 cells, however to a less degree, from basal 133
±
21 to 391
±
98
pg/ml after 72
h viral inoculation. The effects of three extracts on MCP-1 secretion were more complex. FS-t1 displayed both positive and negative effect on virus-stimulated MCP-1 production dependent on the concentrations used. On the other hand, FS-t2 increased virus-induced MCP-1 secretion by 1.4–3.3 times while the third fraction FO-w increased by 2.6–3.7 times. These results suggested that
Forsythia suspense consisted of both negative and positive regulatory components on RANTES and MCP-1 secretion in H1N1-infected A549 cells, respectively.</description><subject>Animals</subject><subject>antiviral agents</subject><subject>Biological and medical sciences</subject><subject>bronchi</subject><subject>Bronchi - metabolism</subject><subject>Bronchi - virology</subject><subject>Cells, Cultured</subject><subject>chemical constituents of plants</subject><subject>Chemokine CCL2 - biosynthesis</subject><subject>Chemokine CCL5 - biosynthesis</subject><subject>chemotaxis</subject><subject>Dogs</subject><subject>epithelial cells</subject><subject>Epithelial Cells - metabolism</subject><subject>Epithelial Cells - virology</subject><subject>ethnobotany</subject><subject>Forsythia</subject><subject>Forsythia suspensa</subject><subject>Forsythia suspense Vahl (Oleaceae)</subject><subject>fruits (plant anatomy)</subject><subject>General pharmacology</subject><subject>herbal medicines</subject><subject>Humans</subject><subject>Influenza A virus</subject><subject>Influenza A Virus, H1N1 Subtype - pathogenicity</subject><subject>Macrophage chemotactic protein-1</subject><subject>macrophages</subject><subject>Medical sciences</subject><subject>medicinal plants</subject><subject>medicinal properties</subject><subject>Normal T cell expressed and secreted</subject><subject>Oriental traditional medicine</subject><subject>Pharmacognosy. Homeopathy. Health food</subject><subject>Pharmacology. Drug treatments</subject><subject>plant extracts</subject><subject>Plant Extracts - pharmacology</subject><subject>protein secretion</subject><subject>Regulated on activation</subject><issn>0378-8741</issn><issn>1872-7573</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kd1u1DAQhSMEokvhAbgB38BdlnHin6y4Wi0tIJUf0fbamjjjrlfZJLWTiuVVeFkc7Uq9Q7Jka_zNmdE5Wfaaw5IDVx92yx0NywJALkEtoVg9yRa80kWupS6fZgsodZVXWvCz7EWMOwDQXMDz7IwrkKLk5SL7-2nClgW6m1oc-3Bg5BzZkfWODS12I6PfY0A7xrly2Yd4GLceWZziQF0k1nfs1_r7zcU1w65h3zY_c84i2UCjT19-Pq6dqPuDbM0efJhinippAjVsO-2xY3XoO5s0W0aDH7fUzk9LbRtfZs8ctpFene7z7Pby4mbzJb_68fnrZn2VWwFyzJtVZWtJqKBAXqPDpgIhlJNCklZ1CdUKneSVXUlwwimHqkyW1RKFdaCb8jx7f9QdQn8_URzN3sd5A-yon6JRldYrrUQC-RG0oY8xkDND8HsMB8PBzImYnUmJmDkRA8qkRFLPm5P4VO-peew4RZCAdycAo8XWBeysj4-cLjRoBYl7e-Qc9gbvQmJurwvgJXAQUmmViI9HgpJZD56CidZTZ6nxITlumt7_Z9F_hGm0Mg</recordid><startdate>20051201</startdate><enddate>20051201</enddate><creator>Ko, Han-Chieh</creator><creator>Wei, Bai-Luh</creator><creator>Chiou, Wen-Fei</creator><general>Elsevier Ireland Ltd</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20051201</creationdate><title>Dual regulatory effect of plant extracts of Forsythia suspense on RANTES and MCP-1 secretion in influenza A virus-infected human bronchial epithelial cells</title><author>Ko, Han-Chieh ; Wei, Bai-Luh ; Chiou, Wen-Fei</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c405t-d98cb5ea602a1bafad80446f545e76b3089af518c950f4f6fa63005b5a4cf07d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Animals</topic><topic>antiviral agents</topic><topic>Biological and medical sciences</topic><topic>bronchi</topic><topic>Bronchi - metabolism</topic><topic>Bronchi - virology</topic><topic>Cells, Cultured</topic><topic>chemical constituents of plants</topic><topic>Chemokine CCL2 - biosynthesis</topic><topic>Chemokine CCL5 - biosynthesis</topic><topic>chemotaxis</topic><topic>Dogs</topic><topic>epithelial cells</topic><topic>Epithelial Cells - metabolism</topic><topic>Epithelial Cells - virology</topic><topic>ethnobotany</topic><topic>Forsythia</topic><topic>Forsythia suspensa</topic><topic>Forsythia suspense Vahl (Oleaceae)</topic><topic>fruits (plant anatomy)</topic><topic>General pharmacology</topic><topic>herbal medicines</topic><topic>Humans</topic><topic>Influenza A virus</topic><topic>Influenza A Virus, H1N1 Subtype - pathogenicity</topic><topic>Macrophage chemotactic protein-1</topic><topic>macrophages</topic><topic>Medical sciences</topic><topic>medicinal plants</topic><topic>medicinal properties</topic><topic>Normal T cell expressed and secreted</topic><topic>Oriental traditional medicine</topic><topic>Pharmacognosy. Homeopathy. Health food</topic><topic>Pharmacology. Drug treatments</topic><topic>plant extracts</topic><topic>Plant Extracts - pharmacology</topic><topic>protein secretion</topic><topic>Regulated on activation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ko, Han-Chieh</creatorcontrib><creatorcontrib>Wei, Bai-Luh</creatorcontrib><creatorcontrib>Chiou, Wen-Fei</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of ethnopharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ko, Han-Chieh</au><au>Wei, Bai-Luh</au><au>Chiou, Wen-Fei</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Dual regulatory effect of plant extracts of Forsythia suspense on RANTES and MCP-1 secretion in influenza A virus-infected human bronchial epithelial cells</atitle><jtitle>Journal of ethnopharmacology</jtitle><addtitle>J Ethnopharmacol</addtitle><date>2005-12-01</date><risdate>2005</risdate><volume>102</volume><issue>3</issue><spage>418</spage><epage>423</epage><pages>418-423</pages><issn>0378-8741</issn><eissn>1872-7573</eissn><coden>JOETD7</coden><abstract>In this study, we investigated the effects of 95% ethanol (FS-t1), 50% ethanol (FS-t2) and water (FS-w) extracts of
Forsythia suspense Vahl (Oleaceae) on the production of regulated on activation, normal T cell expressed and secreted (RANTES) and macrophage chemotactic protein-1 (MCP-1) by influenza A virus (H1N1)-infected human bronchial epithelial cell line A549. Virus infection evoked a markedly enhanced production of RANTES from basal 16
±
4 to 1307
±
294
pg/ml after 72
h inoculation. At the non-cytotoxic doses (20, 100 and 200
μg/ml), FS-t1, FS-t2 and FS-w exhibited a consistent inhibitory effect on virus-stimulated RANTES secretion in a dose-dependent manner wilh IC
50 of 42
±
6, 117
±
15 and 232
±
28
μg/ml, respectively. H1N1 also stimulated MCP-1 production in A549 cells, however to a less degree, from basal 133
±
21 to 391
±
98
pg/ml after 72
h viral inoculation. The effects of three extracts on MCP-1 secretion were more complex. FS-t1 displayed both positive and negative effect on virus-stimulated MCP-1 production dependent on the concentrations used. On the other hand, FS-t2 increased virus-induced MCP-1 secretion by 1.4–3.3 times while the third fraction FO-w increased by 2.6–3.7 times. These results suggested that
Forsythia suspense consisted of both negative and positive regulatory components on RANTES and MCP-1 secretion in H1N1-infected A549 cells, respectively.</abstract><cop>Shannon</cop><pub>Elsevier Ireland Ltd</pub><pmid>16054313</pmid><doi>10.1016/j.jep.2005.06.029</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Journal of ethnopharmacology, 2005-12, Vol.102 (3), p.418-423 |
| issn | 0378-8741 1872-7573 |
| language | eng |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Animals antiviral agents Biological and medical sciences bronchi Bronchi - metabolism Bronchi - virology Cells, Cultured chemical constituents of plants Chemokine CCL2 - biosynthesis Chemokine CCL5 - biosynthesis chemotaxis Dogs epithelial cells Epithelial Cells - metabolism Epithelial Cells - virology ethnobotany Forsythia Forsythia suspensa Forsythia suspense Vahl (Oleaceae) fruits (plant anatomy) General pharmacology herbal medicines Humans Influenza A virus Influenza A Virus, H1N1 Subtype - pathogenicity Macrophage chemotactic protein-1 macrophages Medical sciences medicinal plants medicinal properties Normal T cell expressed and secreted Oriental traditional medicine Pharmacognosy. Homeopathy. Health food Pharmacology. Drug treatments plant extracts Plant Extracts - pharmacology protein secretion Regulated on activation |
| title | Dual regulatory effect of plant extracts of Forsythia suspense on RANTES and MCP-1 secretion in influenza A virus-infected human bronchial epithelial cells |
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