Na+/K+ATPase as a Signaling Molecule During Bovine Sperm Capacitation
A heteromeric integral membrane protein, Na + /K + ATPase is composed of two polypeptides, alpha and beta, and is active in many cell types, including testis and spermatozoa. It is a well-known ion transporter, but binding of ouabain, a specific inhibitor of Na + /K + ATPase, to Na + /K + ATPase in...
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| Veröffentlicht in: | Biology of reproduction 2006-09, Vol.75 (3), p.308-317 |
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| description | A heteromeric integral membrane protein, Na + /K + ATPase is composed of two polypeptides, alpha and beta, and is active in many cell types, including testis and spermatozoa.
It is a well-known ion transporter, but binding of ouabain, a specific inhibitor of Na + /K + ATPase, to Na + /K + ATPase in somatic cells initiates responses that are similar to signaling events associated with bovine sperm capacitation.
The objectives of the present study were to demonstrate the presence of Na + /K + ATPase in bovine sperm and to investigate its role in the regulation of bovine sperm capacitation. The presence of Na + /K + ATPase in sperm from mature Holstein bulls was demonstrated by immunoblotting and immunocytochemistry using a monoclonal antibody
developed in mouse against the beta 1 polypeptide of Na + /K + ATPase. Binding of ouabain to Na + /K + ATPase inhibited motility (decreased progressive motility, average path velocity, and curvilinear velocity) and induced tyrosine
phosphorylation and capacitation but did not increase intracellular calcium levels in spermatozoa. Furthermore, binding of
ouabain to Na + /K + ATPase induced depolarization of sperm plasma membrane. Therefore, binding of ouabain to Na + /K + ATPase induced sperm capacitation through depolarization of sperm plasma membrane and signaling via the tyrosine phosphorylation
pathway without an appreciable increase in intracellular calcium. To our knowledge, this is the first report concerning the
signaling role of Na + /K + ATPase in mammalian sperm capacitation.
Abstract
Na + /K + ATPase is involved in the regulation of protein tyrosine phosphorylation and capacitation of bovine spermatozoa. |
| doi_str_mv | 10.1095/biolreprod.105.047852 |
| format | Article |
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It is a well-known ion transporter, but binding of ouabain, a specific inhibitor of Na + /K + ATPase, to Na + /K + ATPase in somatic cells initiates responses that are similar to signaling events associated with bovine sperm capacitation.
The objectives of the present study were to demonstrate the presence of Na + /K + ATPase in bovine sperm and to investigate its role in the regulation of bovine sperm capacitation. The presence of Na + /K + ATPase in sperm from mature Holstein bulls was demonstrated by immunoblotting and immunocytochemistry using a monoclonal antibody
developed in mouse against the beta 1 polypeptide of Na + /K + ATPase. Binding of ouabain to Na + /K + ATPase inhibited motility (decreased progressive motility, average path velocity, and curvilinear velocity) and induced tyrosine
phosphorylation and capacitation but did not increase intracellular calcium levels in spermatozoa. Furthermore, binding of
ouabain to Na + /K + ATPase induced depolarization of sperm plasma membrane. Therefore, binding of ouabain to Na + /K + ATPase induced sperm capacitation through depolarization of sperm plasma membrane and signaling via the tyrosine phosphorylation
pathway without an appreciable increase in intracellular calcium. To our knowledge, this is the first report concerning the
signaling role of Na + /K + ATPase in mammalian sperm capacitation.
Abstract
Na + /K + ATPase is involved in the regulation of protein tyrosine phosphorylation and capacitation of bovine spermatozoa.</description><identifier>ISSN: 0006-3363</identifier><identifier>EISSN: 1529-7268</identifier><identifier>DOI: 10.1095/biolreprod.105.047852</identifier><identifier>PMID: 16687652</identifier><identifier>CODEN: BIREBV</identifier><language>eng</language><publisher>Madison, WI: Society for the Study of Reproduction</publisher><subject>Animals ; Biological and medical sciences ; Calcium - metabolism ; Cattle ; Cell Membrane - physiology ; Enzyme Inhibitors - pharmacology ; Fundamental and applied biological sciences. Psychology ; Immunohistochemistry ; In Vitro Techniques ; Indicators and Reagents ; Male ; Mammalian male genital system ; Membrane Potentials - physiology ; Morphology. Physiology ; Ouabain - pharmacology ; Phosphorylation ; Signal Transduction - physiology ; Sodium-Potassium-Exchanging ATPase - antagonists & inhibitors ; Sodium-Potassium-Exchanging ATPase - metabolism ; Sodium-Potassium-Exchanging ATPase - physiology ; Sperm Capacitation - physiology ; Sperm Motility - physiology ; Spermatozoa - enzymology ; Tyrosine - metabolism ; Vertebrates: reproduction</subject><ispartof>Biology of reproduction, 2006-09, Vol.75 (3), p.308-317</ispartof><rights>2006 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18058810$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16687652$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>THUNDATHIL, Jacob C</creatorcontrib><creatorcontrib>ANZAR, Muhammad</creatorcontrib><creatorcontrib>BUHR, Mary M</creatorcontrib><title>Na+/K+ATPase as a Signaling Molecule During Bovine Sperm Capacitation</title><title>Biology of reproduction</title><addtitle>Biol Reprod</addtitle><description>A heteromeric integral membrane protein, Na + /K + ATPase is composed of two polypeptides, alpha and beta, and is active in many cell types, including testis and spermatozoa.
It is a well-known ion transporter, but binding of ouabain, a specific inhibitor of Na + /K + ATPase, to Na + /K + ATPase in somatic cells initiates responses that are similar to signaling events associated with bovine sperm capacitation.
The objectives of the present study were to demonstrate the presence of Na + /K + ATPase in bovine sperm and to investigate its role in the regulation of bovine sperm capacitation. The presence of Na + /K + ATPase in sperm from mature Holstein bulls was demonstrated by immunoblotting and immunocytochemistry using a monoclonal antibody
developed in mouse against the beta 1 polypeptide of Na + /K + ATPase. Binding of ouabain to Na + /K + ATPase inhibited motility (decreased progressive motility, average path velocity, and curvilinear velocity) and induced tyrosine
phosphorylation and capacitation but did not increase intracellular calcium levels in spermatozoa. Furthermore, binding of
ouabain to Na + /K + ATPase induced depolarization of sperm plasma membrane. Therefore, binding of ouabain to Na + /K + ATPase induced sperm capacitation through depolarization of sperm plasma membrane and signaling via the tyrosine phosphorylation
pathway without an appreciable increase in intracellular calcium. To our knowledge, this is the first report concerning the
signaling role of Na + /K + ATPase in mammalian sperm capacitation.
Abstract
Na + /K + ATPase is involved in the regulation of protein tyrosine phosphorylation and capacitation of bovine spermatozoa.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Calcium - metabolism</subject><subject>Cattle</subject><subject>Cell Membrane - physiology</subject><subject>Enzyme Inhibitors - pharmacology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Immunohistochemistry</subject><subject>In Vitro Techniques</subject><subject>Indicators and Reagents</subject><subject>Male</subject><subject>Mammalian male genital system</subject><subject>Membrane Potentials - physiology</subject><subject>Morphology. Physiology</subject><subject>Ouabain - pharmacology</subject><subject>Phosphorylation</subject><subject>Signal Transduction - physiology</subject><subject>Sodium-Potassium-Exchanging ATPase - antagonists & inhibitors</subject><subject>Sodium-Potassium-Exchanging ATPase - metabolism</subject><subject>Sodium-Potassium-Exchanging ATPase - physiology</subject><subject>Sperm Capacitation - physiology</subject><subject>Sperm Motility - physiology</subject><subject>Spermatozoa - enzymology</subject><subject>Tyrosine - metabolism</subject><subject>Vertebrates: reproduction</subject><issn>0006-3363</issn><issn>1529-7268</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFj8lOwzAURS0EoqXwCaBsYFOl9RAPWZZSBlEGqWUdvcZOa-QMxA0Vf48RRazeu9LR1T0InRM8Ijjl45WtXWuattYh8xFOpOL0APUJp2ksqVCHqI8xFjFjgvXQiffvGJOEUXaMekQIJQWnfTR7huH4cThZvoI3EfgIooVdV-BstY6eamfyzpnopmt_8nX9aSsTLRrTltEUGsjtFra2rk7RUQHOm7P9HaC329lyeh_PX-4eppN5vKEi3cYp0VwpSCCn2uiEUhweUgijw-hcE01IIZkGLCTmeaopJ9IQSYiiKWdasQG6-u0N3h-d8dustD43zkFl6s5nwUqmQvEAXuzBblUanTWtLaH9yv7EA3C5B8Dn4IoWqtz6f07hsJTgf25j15udbU3mS3Au1LJst9tJnrGMYcW-AWOPdQw</recordid><startdate>20060901</startdate><enddate>20060901</enddate><creator>THUNDATHIL, Jacob C</creator><creator>ANZAR, Muhammad</creator><creator>BUHR, Mary M</creator><general>Society for the Study of Reproduction</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20060901</creationdate><title>Na+/K+ATPase as a Signaling Molecule During Bovine Sperm Capacitation</title><author>THUNDATHIL, Jacob C ; ANZAR, Muhammad ; BUHR, Mary M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h269t-91d588a4ac2ded4220c2d1f6ed529cd1d11f73da06705c9d2517e171182953d83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Calcium - metabolism</topic><topic>Cattle</topic><topic>Cell Membrane - physiology</topic><topic>Enzyme Inhibitors - pharmacology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Immunohistochemistry</topic><topic>In Vitro Techniques</topic><topic>Indicators and Reagents</topic><topic>Male</topic><topic>Mammalian male genital system</topic><topic>Membrane Potentials - physiology</topic><topic>Morphology. Physiology</topic><topic>Ouabain - pharmacology</topic><topic>Phosphorylation</topic><topic>Signal Transduction - physiology</topic><topic>Sodium-Potassium-Exchanging ATPase - antagonists & inhibitors</topic><topic>Sodium-Potassium-Exchanging ATPase - metabolism</topic><topic>Sodium-Potassium-Exchanging ATPase - physiology</topic><topic>Sperm Capacitation - physiology</topic><topic>Sperm Motility - physiology</topic><topic>Spermatozoa - enzymology</topic><topic>Tyrosine - metabolism</topic><topic>Vertebrates: reproduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>THUNDATHIL, Jacob C</creatorcontrib><creatorcontrib>ANZAR, Muhammad</creatorcontrib><creatorcontrib>BUHR, Mary M</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Biology of reproduction</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>THUNDATHIL, Jacob C</au><au>ANZAR, Muhammad</au><au>BUHR, Mary M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Na+/K+ATPase as a Signaling Molecule During Bovine Sperm Capacitation</atitle><jtitle>Biology of reproduction</jtitle><addtitle>Biol Reprod</addtitle><date>2006-09-01</date><risdate>2006</risdate><volume>75</volume><issue>3</issue><spage>308</spage><epage>317</epage><pages>308-317</pages><issn>0006-3363</issn><eissn>1529-7268</eissn><coden>BIREBV</coden><abstract>A heteromeric integral membrane protein, Na + /K + ATPase is composed of two polypeptides, alpha and beta, and is active in many cell types, including testis and spermatozoa.
It is a well-known ion transporter, but binding of ouabain, a specific inhibitor of Na + /K + ATPase, to Na + /K + ATPase in somatic cells initiates responses that are similar to signaling events associated with bovine sperm capacitation.
The objectives of the present study were to demonstrate the presence of Na + /K + ATPase in bovine sperm and to investigate its role in the regulation of bovine sperm capacitation. The presence of Na + /K + ATPase in sperm from mature Holstein bulls was demonstrated by immunoblotting and immunocytochemistry using a monoclonal antibody
developed in mouse against the beta 1 polypeptide of Na + /K + ATPase. Binding of ouabain to Na + /K + ATPase inhibited motility (decreased progressive motility, average path velocity, and curvilinear velocity) and induced tyrosine
phosphorylation and capacitation but did not increase intracellular calcium levels in spermatozoa. Furthermore, binding of
ouabain to Na + /K + ATPase induced depolarization of sperm plasma membrane. Therefore, binding of ouabain to Na + /K + ATPase induced sperm capacitation through depolarization of sperm plasma membrane and signaling via the tyrosine phosphorylation
pathway without an appreciable increase in intracellular calcium. To our knowledge, this is the first report concerning the
signaling role of Na + /K + ATPase in mammalian sperm capacitation.
Abstract
Na + /K + ATPase is involved in the regulation of protein tyrosine phosphorylation and capacitation of bovine spermatozoa.</abstract><cop>Madison, WI</cop><pub>Society for the Study of Reproduction</pub><pmid>16687652</pmid><doi>10.1095/biolreprod.105.047852</doi><tpages>10</tpages></addata></record> |
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| source | MEDLINE; BioOne Complete; Oxford University Press Journals All Titles (1996-Current); EZB-FREE-00999 freely available EZB journals |
| subjects | Animals Biological and medical sciences Calcium - metabolism Cattle Cell Membrane - physiology Enzyme Inhibitors - pharmacology Fundamental and applied biological sciences. Psychology Immunohistochemistry In Vitro Techniques Indicators and Reagents Male Mammalian male genital system Membrane Potentials - physiology Morphology. Physiology Ouabain - pharmacology Phosphorylation Signal Transduction - physiology Sodium-Potassium-Exchanging ATPase - antagonists & inhibitors Sodium-Potassium-Exchanging ATPase - metabolism Sodium-Potassium-Exchanging ATPase - physiology Sperm Capacitation - physiology Sperm Motility - physiology Spermatozoa - enzymology Tyrosine - metabolism Vertebrates: reproduction |
| title | Na+/K+ATPase as a Signaling Molecule During Bovine Sperm Capacitation |
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