K+ waves in brain cortex visualized using a long-wavelength K+-sensing fluorescent indicator
We synthesized a water-soluble, long-wavelength K + sensor, TAC-Red, consisting of triazacryptand coupled to 3,6-bis(dimethylamino)xanthylium, whose fluorescence increased 14-fold at 0–50 mM K + with K + -to-Na + selectivity >30. We visualized K + waves in TAC-Red–stained brain cortex in mice dur...
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| Veröffentlicht in: | Nature methods 2005-11, Vol.2 (11), p.825-827 |
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| creator | Padmawar, Prashant Yao, Xiaoming Bloch, Orin Manley, Geoffrey T Verkman, A S |
| description | We synthesized a water-soluble, long-wavelength K
+
sensor, TAC-Red, consisting of triazacryptand coupled to 3,6-bis(dimethylamino)xanthylium, whose fluorescence increased 14-fold at 0–50 mM K
+
with K
+
-to-Na
+
selectivity >30. We visualized K
+
waves in TAC-Red–stained brain cortex in mice during spreading depression, with velocity 4.4 ± 0.5 mm/min, and K
+
release and reuptake half-times (
t
1/2
) of 12 ± 2 and 32 ± 4 s, respectively. Aquaporin-4 (AQP4) deletion slowed K
+
reuptake about twofold, suggesting AQP4-dependent K
+
uptake by astroglia. |
| doi_str_mv | 10.1038/nmeth801 |
| format | Article |
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+
sensor, TAC-Red, consisting of triazacryptand coupled to 3,6-bis(dimethylamino)xanthylium, whose fluorescence increased 14-fold at 0–50 mM K
+
with K
+
-to-Na
+
selectivity >30. We visualized K
+
waves in TAC-Red–stained brain cortex in mice during spreading depression, with velocity 4.4 ± 0.5 mm/min, and K
+
release and reuptake half-times (
t
1/2
) of 12 ± 2 and 32 ± 4 s, respectively. Aquaporin-4 (AQP4) deletion slowed K
+
reuptake about twofold, suggesting AQP4-dependent K
+
uptake by astroglia.</description><identifier>ISSN: 1548-7091</identifier><identifier>EISSN: 1548-7105</identifier><identifier>DOI: 10.1038/nmeth801</identifier><identifier>PMID: 16278651</identifier><language>eng</language><publisher>New York: Nature Publishing Group US</publisher><subject>Analysis ; Animals ; Aquaporin 4 - genetics ; Aquaporin 4 - metabolism ; Astrocytes - metabolism ; Aza Compounds - analysis ; Aza Compounds - chemistry ; Bioinformatics ; Biological Microscopy ; Biological Techniques ; Biomedical and Life Sciences ; Biomedical Engineering/Biotechnology ; brief-communication ; Cerebral Cortex - metabolism ; Fluorescence ; Fluorescent antibody technique ; Fluorescent Dyes - analysis ; Fluorescent Dyes - chemistry ; Heterocyclic Compounds, 3-Ring - analysis ; Heterocyclic Compounds, 3-Ring - chemistry ; Immunofluorescence ; Life Sciences ; Mice ; Mice, Knockout ; Molecular Structure ; Potassium - analysis ; Potassium - metabolism ; Potassium channels ; Proteomics ; Solubility ; Time Factors</subject><ispartof>Nature methods, 2005-11, Vol.2 (11), p.825-827</ispartof><rights>Springer Nature America, Inc. 2005</rights><rights>COPYRIGHT 2005 Nature Publishing Group</rights><rights>Copyright Nature Publishing Group Nov 2005</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c447t-d1e432bcc301ff0e9483069079ea7bb8a056201a19f59681ecdfd0460a379953</citedby><cites>FETCH-LOGICAL-c447t-d1e432bcc301ff0e9483069079ea7bb8a056201a19f59681ecdfd0460a379953</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1038/nmeth801$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1038/nmeth801$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,777,781,27905,27906,41469,42538,51300</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16278651$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Padmawar, Prashant</creatorcontrib><creatorcontrib>Yao, Xiaoming</creatorcontrib><creatorcontrib>Bloch, Orin</creatorcontrib><creatorcontrib>Manley, Geoffrey T</creatorcontrib><creatorcontrib>Verkman, A S</creatorcontrib><title>K+ waves in brain cortex visualized using a long-wavelength K+-sensing fluorescent indicator</title><title>Nature methods</title><addtitle>Nat Methods</addtitle><addtitle>Nat Methods</addtitle><description>We synthesized a water-soluble, long-wavelength K
+
sensor, TAC-Red, consisting of triazacryptand coupled to 3,6-bis(dimethylamino)xanthylium, whose fluorescence increased 14-fold at 0–50 mM K
+
with K
+
-to-Na
+
selectivity >30. We visualized K
+
waves in TAC-Red–stained brain cortex in mice during spreading depression, with velocity 4.4 ± 0.5 mm/min, and K
+
release and reuptake half-times (
t
1/2
) of 12 ± 2 and 32 ± 4 s, respectively. Aquaporin-4 (AQP4) deletion slowed K
+
reuptake about twofold, suggesting AQP4-dependent K
+
uptake by astroglia.</description><subject>Analysis</subject><subject>Animals</subject><subject>Aquaporin 4 - genetics</subject><subject>Aquaporin 4 - metabolism</subject><subject>Astrocytes - metabolism</subject><subject>Aza Compounds - analysis</subject><subject>Aza Compounds - chemistry</subject><subject>Bioinformatics</subject><subject>Biological Microscopy</subject><subject>Biological Techniques</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedical Engineering/Biotechnology</subject><subject>brief-communication</subject><subject>Cerebral Cortex - metabolism</subject><subject>Fluorescence</subject><subject>Fluorescent antibody technique</subject><subject>Fluorescent Dyes - analysis</subject><subject>Fluorescent Dyes - chemistry</subject><subject>Heterocyclic Compounds, 3-Ring - analysis</subject><subject>Heterocyclic Compounds, 3-Ring - chemistry</subject><subject>Immunofluorescence</subject><subject>Life Sciences</subject><subject>Mice</subject><subject>Mice, Knockout</subject><subject>Molecular Structure</subject><subject>Potassium - analysis</subject><subject>Potassium - metabolism</subject><subject>Potassium channels</subject><subject>Proteomics</subject><subject>Solubility</subject><subject>Time 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metabolism</topic><topic>Fluorescence</topic><topic>Fluorescent antibody technique</topic><topic>Fluorescent Dyes - analysis</topic><topic>Fluorescent Dyes - chemistry</topic><topic>Heterocyclic Compounds, 3-Ring - analysis</topic><topic>Heterocyclic Compounds, 3-Ring - chemistry</topic><topic>Immunofluorescence</topic><topic>Life Sciences</topic><topic>Mice</topic><topic>Mice, Knockout</topic><topic>Molecular Structure</topic><topic>Potassium - analysis</topic><topic>Potassium - metabolism</topic><topic>Potassium channels</topic><topic>Proteomics</topic><topic>Solubility</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Padmawar, Prashant</creatorcontrib><creatorcontrib>Yao, Xiaoming</creatorcontrib><creatorcontrib>Bloch, Orin</creatorcontrib><creatorcontrib>Manley, Geoffrey T</creatorcontrib><creatorcontrib>Verkman, A 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Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Nature methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Padmawar, Prashant</au><au>Yao, Xiaoming</au><au>Bloch, Orin</au><au>Manley, Geoffrey T</au><au>Verkman, A S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>K+ waves in brain cortex visualized using a long-wavelength K+-sensing fluorescent indicator</atitle><jtitle>Nature methods</jtitle><stitle>Nat Methods</stitle><addtitle>Nat Methods</addtitle><date>2005-11-01</date><risdate>2005</risdate><volume>2</volume><issue>11</issue><spage>825</spage><epage>827</epage><pages>825-827</pages><issn>1548-7091</issn><eissn>1548-7105</eissn><abstract>We synthesized a water-soluble, long-wavelength K
+
sensor, TAC-Red, consisting of triazacryptand coupled to 3,6-bis(dimethylamino)xanthylium, whose fluorescence increased 14-fold at 0–50 mM K
+
with K
+
-to-Na
+
selectivity >30. We visualized K
+
waves in TAC-Red–stained brain cortex in mice during spreading depression, with velocity 4.4 ± 0.5 mm/min, and K
+
release and reuptake half-times (
t
1/2
) of 12 ± 2 and 32 ± 4 s, respectively. Aquaporin-4 (AQP4) deletion slowed K
+
reuptake about twofold, suggesting AQP4-dependent K
+
uptake by astroglia.</abstract><cop>New York</cop><pub>Nature Publishing Group US</pub><pmid>16278651</pmid><doi>10.1038/nmeth801</doi><tpages>3</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1548-7091 |
| ispartof | Nature methods, 2005-11, Vol.2 (11), p.825-827 |
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| language | eng |
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| source | MEDLINE; Nature Journals Online; SpringerLink Journals - AutoHoldings |
| subjects | Analysis Animals Aquaporin 4 - genetics Aquaporin 4 - metabolism Astrocytes - metabolism Aza Compounds - analysis Aza Compounds - chemistry Bioinformatics Biological Microscopy Biological Techniques Biomedical and Life Sciences Biomedical Engineering/Biotechnology brief-communication Cerebral Cortex - metabolism Fluorescence Fluorescent antibody technique Fluorescent Dyes - analysis Fluorescent Dyes - chemistry Heterocyclic Compounds, 3-Ring - analysis Heterocyclic Compounds, 3-Ring - chemistry Immunofluorescence Life Sciences Mice Mice, Knockout Molecular Structure Potassium - analysis Potassium - metabolism Potassium channels Proteomics Solubility Time Factors |
| title | K+ waves in brain cortex visualized using a long-wavelength K+-sensing fluorescent indicator |
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