Imitation of artificial membrane system via mobile phases with Tween-80 and cholic acid in biopartitioning micellar chromatography
The chromatographic behaviour of compounds of biomedical significance was studied using micellar mobile phases modified with polyoxyethylene (20) sorbitan monooleate (Tween‐80). The influence of the surfactant within the 0.75–4% concentration range on the retention factor of model compounds was inve...
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| Veröffentlicht in: | Biomedical chromatography 2006-08, Vol.20 (8), p.753-759 |
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| creator | Rukhadze, Marina D. Sebiskveradze, Maya V. Akhalkatsi, Tsaro G. Makharadze, Teona G. |
| description | The chromatographic behaviour of compounds of biomedical significance was studied using micellar mobile phases modified with polyoxyethylene (20) sorbitan monooleate (Tween‐80). The influence of the surfactant within the 0.75–4% concentration range on the retention factor of model compounds was investigated. The biological surfactant cholic acid was introduced into the mobile phases in order to approach to the structure of natural membranes, viz. erythrocyte and cytoplasmatic membranes. It was found that curves of dependence of retention factor vs concentration of Tween‐80 in the absence and presence of cholic acid in the mobile phase considerably diverge with one another, especially in the 2–3% concentration range of Tween‐80 using C18‐type support. Increasing the concentration of Tween‐80 resulted in the increase of retention factors using phenyl‐coated stationary phase. Copyright © 2005 John Wiley & Sons, Ltd. |
| doi_str_mv | 10.1002/bmc.592 |
| format | Article |
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The influence of the surfactant within the 0.75–4% concentration range on the retention factor of model compounds was investigated. The biological surfactant cholic acid was introduced into the mobile phases in order to approach to the structure of natural membranes, viz. erythrocyte and cytoplasmatic membranes. It was found that curves of dependence of retention factor vs concentration of Tween‐80 in the absence and presence of cholic acid in the mobile phase considerably diverge with one another, especially in the 2–3% concentration range of Tween‐80 using C18‐type support. Increasing the concentration of Tween‐80 resulted in the increase of retention factors using phenyl‐coated stationary phase. 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Chromatogr</addtitle><description>The chromatographic behaviour of compounds of biomedical significance was studied using micellar mobile phases modified with polyoxyethylene (20) sorbitan monooleate (Tween‐80). The influence of the surfactant within the 0.75–4% concentration range on the retention factor of model compounds was investigated. The biological surfactant cholic acid was introduced into the mobile phases in order to approach to the structure of natural membranes, viz. erythrocyte and cytoplasmatic membranes. It was found that curves of dependence of retention factor vs concentration of Tween‐80 in the absence and presence of cholic acid in the mobile phase considerably diverge with one another, especially in the 2–3% concentration range of Tween‐80 using C18‐type support. Increasing the concentration of Tween‐80 resulted in the increase of retention factors using phenyl‐coated stationary phase. Copyright © 2005 John Wiley & Sons, Ltd.</description><subject>biopartitioning micellar chromatography</subject><subject>cholic acid</subject><subject>Cholic Acid - chemistry</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Chromatography, Micellar Electrokinetic Capillary - methods</subject><subject>Hydrophobic and Hydrophilic Interactions</subject><subject>hydrophobicity</subject><subject>Membranes, Artificial</subject><subject>Micelles</subject><subject>Polysorbates - chemistry</subject><subject>Tween-80</subject><subject>Vitamins - isolation & purification</subject><issn>0269-3879</issn><issn>1099-0801</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kM1u1DAYRS0EokNBvAHyChYoxY7Hf0tIaanUwqYIdtYXx-kY4ji1Mwyz5cnxkBGsWN3N0dHVQeg5JWeUkPpNG-wZ1_UDtKJE64ooQh-iFamFrpiS-gQ9yfkbIUSLWj5GJ7SM4Jys0K-r4GeYfRxx7DGk2ffeehhwcKFNMDqc93l2Af_wgENs_eDwtIHsMt75eYNvd86NlSIYxg7bTRy8xWB9h_2IWx-ng_Fg9-MdDt66YYBUuBQDzPEuwbTZP0WPehiye3bcU_T54v1t86G6_nR51by9rizjrK4sONn3PXWWSSoYpbrma6UIWTMhiKBdR3nvKAhFrQKg3NF1q9dAJRe21YqdopeLd0rxfuvybILPfx6NLm6zEUpKUeoV8NUC2hRzTq43U_IB0t5QYg65TcltSu5Cvjgqt21w3T_u2LcArxdgV8Lt_-cx726aRVcttC_Jf_6lIX03QjLJzZePl-b868W5uFGNadhvq6qZFQ</recordid><startdate>200608</startdate><enddate>200608</enddate><creator>Rukhadze, Marina D.</creator><creator>Sebiskveradze, Maya V.</creator><creator>Akhalkatsi, Tsaro G.</creator><creator>Makharadze, Teona G.</creator><general>John Wiley & Sons, Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200608</creationdate><title>Imitation of artificial membrane system via mobile phases with Tween-80 and cholic acid in biopartitioning micellar chromatography</title><author>Rukhadze, Marina D. ; Sebiskveradze, Maya V. ; Akhalkatsi, Tsaro G. ; Makharadze, Teona G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3532-cae7fff1ec3716311925488004366061dd15fe1a681c8aa15e14b94a1756cb983</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>biopartitioning micellar chromatography</topic><topic>cholic acid</topic><topic>Cholic Acid - chemistry</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Chromatography, Micellar Electrokinetic Capillary - methods</topic><topic>Hydrophobic and Hydrophilic Interactions</topic><topic>hydrophobicity</topic><topic>Membranes, Artificial</topic><topic>Micelles</topic><topic>Polysorbates - chemistry</topic><topic>Tween-80</topic><topic>Vitamins - isolation & purification</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rukhadze, Marina D.</creatorcontrib><creatorcontrib>Sebiskveradze, Maya V.</creatorcontrib><creatorcontrib>Akhalkatsi, Tsaro G.</creatorcontrib><creatorcontrib>Makharadze, Teona G.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biomedical chromatography</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rukhadze, Marina D.</au><au>Sebiskveradze, Maya V.</au><au>Akhalkatsi, Tsaro G.</au><au>Makharadze, Teona G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Imitation of artificial membrane system via mobile phases with Tween-80 and cholic acid in biopartitioning micellar chromatography</atitle><jtitle>Biomedical chromatography</jtitle><addtitle>Biomed. Chromatogr</addtitle><date>2006-08</date><risdate>2006</risdate><volume>20</volume><issue>8</issue><spage>753</spage><epage>759</epage><pages>753-759</pages><issn>0269-3879</issn><eissn>1099-0801</eissn><abstract>The chromatographic behaviour of compounds of biomedical significance was studied using micellar mobile phases modified with polyoxyethylene (20) sorbitan monooleate (Tween‐80). The influence of the surfactant within the 0.75–4% concentration range on the retention factor of model compounds was investigated. The biological surfactant cholic acid was introduced into the mobile phases in order to approach to the structure of natural membranes, viz. erythrocyte and cytoplasmatic membranes. It was found that curves of dependence of retention factor vs concentration of Tween‐80 in the absence and presence of cholic acid in the mobile phase considerably diverge with one another, especially in the 2–3% concentration range of Tween‐80 using C18‐type support. Increasing the concentration of Tween‐80 resulted in the increase of retention factors using phenyl‐coated stationary phase. Copyright © 2005 John Wiley & Sons, Ltd.</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><pmid>16276550</pmid><doi>10.1002/bmc.592</doi><tpages>7</tpages></addata></record> |
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| ispartof | Biomedical chromatography, 2006-08, Vol.20 (8), p.753-759 |
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| language | eng |
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| source | MEDLINE; Access via Wiley Online Library |
| subjects | biopartitioning micellar chromatography cholic acid Cholic Acid - chemistry Chromatography, High Pressure Liquid Chromatography, Micellar Electrokinetic Capillary - methods Hydrophobic and Hydrophilic Interactions hydrophobicity Membranes, Artificial Micelles Polysorbates - chemistry Tween-80 Vitamins - isolation & purification |
| title | Imitation of artificial membrane system via mobile phases with Tween-80 and cholic acid in biopartitioning micellar chromatography |
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