Magnesium ion regulation of in vitro rubber biosynthesis by Parthenium argentatum Gray
The rate of rubber biosynthesis and the molecular weight of the rubber produced are both strongly affected by [Mg 2+] in Parthenium argentatum. Natural rubber is produced by a rubber transferase (a cis-prenyltransferase). Rubber transferase uses allylic pyrophosphate to initiate the rubber molecule...
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| Veröffentlicht in: | Phytochemistry (Oxford) 2006-08, Vol.67 (15), p.1621-1628 |
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| creator | da Costa, Bernardo M.T. Keasling, Jay D. McMahan, Colleen M. Cornish, Katrina |
| description | The rate of rubber biosynthesis and the molecular weight of the rubber produced are both strongly affected by [Mg
2+] in
Parthenium argentatum.
Natural rubber is produced by a rubber transferase (a
cis-prenyltransferase). Rubber transferase uses allylic pyrophosphate to initiate the rubber molecule and isopentenyl pyrophosphate (IPP) to form the polymer. Rubber biosynthesis also requires a divalent metal cation. Understanding how molecular weight is regulated is important because high molecular weight is required for high quality rubber. We characterized the in vitro effects of Mg
2+ on the biosynthetic rate of rubber produced by an alternative natural rubber crop,
Parthenium argentatum (guayule). The affinity of the rubber transferase from
P. argentatum for IPP
·
Mg was shown to depend on the Mg
2+ concentration in a similar fashion to the
H. brasiliensis rubber transferase, although to a less extreme degree. Also, in vitro Mg
2+ concentration significantly affects rubber molecular weight of both species, but molecular weight is less sensitive to Mg
2+ concentration in
P. argentatum than in
H. brasiliensis. |
| doi_str_mv | 10.1016/j.phytochem.2006.04.010 |
| format | Article |
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2+] in
Parthenium argentatum.
Natural rubber is produced by a rubber transferase (a
cis-prenyltransferase). Rubber transferase uses allylic pyrophosphate to initiate the rubber molecule and isopentenyl pyrophosphate (IPP) to form the polymer. Rubber biosynthesis also requires a divalent metal cation. Understanding how molecular weight is regulated is important because high molecular weight is required for high quality rubber. We characterized the in vitro effects of Mg
2+ on the biosynthetic rate of rubber produced by an alternative natural rubber crop,
Parthenium argentatum (guayule). The affinity of the rubber transferase from
P. argentatum for IPP
·
Mg was shown to depend on the Mg
2+ concentration in a similar fashion to the
H. brasiliensis rubber transferase, although to a less extreme degree. Also, in vitro Mg
2+ concentration significantly affects rubber molecular weight of both species, but molecular weight is less sensitive to Mg
2+ concentration in
P. argentatum than in
H. brasiliensis.</description><identifier>ISSN: 0031-9422</identifier><identifier>EISSN: 1873-3700</identifier><identifier>DOI: 10.1016/j.phytochem.2006.04.010</identifier><identifier>PMID: 16780905</identifier><language>eng</language><publisher>Amsterdam: Elsevier Ltd</publisher><subject>alkyl (aryl) transferases ; Asteraceae ; Asteraceae - metabolism ; Biological and medical sciences ; biosynthesis ; cis-prenyl transferase ; cis-Prenyltransferase ; Compositae ; enzyme activity ; Euphorbiaceae ; farnesyl pyrophosphate ; FPP ; Fundamental and applied biological sciences. Psychology ; Hevea brasiliensis ; Hevea brasiliensis Muell. Arg ; IPP ; isopentenyl pyrophosphate ; Kinetics ; Magnesium ; Magnesium - pharmacology ; Metabolism ; Metabolism. Physicochemical requirements ; metal ions ; Molecular Weight ; Parthenium argentatum ; Plant physiology and development ; Regulation ; Rubber ; rubber prenyltransferase ; Rubber transferase</subject><ispartof>Phytochemistry (Oxford), 2006-08, Vol.67 (15), p.1621-1628</ispartof><rights>2006 Elsevier Ltd</rights><rights>2006 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c423t-5030a0f7a25255ca84f7f3e7e4f6d7ebd79261553aeb8b809f90f5c8205f71013</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.phytochem.2006.04.010$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18074833$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16780905$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>da Costa, Bernardo M.T.</creatorcontrib><creatorcontrib>Keasling, Jay D.</creatorcontrib><creatorcontrib>McMahan, Colleen M.</creatorcontrib><creatorcontrib>Cornish, Katrina</creatorcontrib><title>Magnesium ion regulation of in vitro rubber biosynthesis by Parthenium argentatum Gray</title><title>Phytochemistry (Oxford)</title><addtitle>Phytochemistry</addtitle><description>The rate of rubber biosynthesis and the molecular weight of the rubber produced are both strongly affected by [Mg
2+] in
Parthenium argentatum.
Natural rubber is produced by a rubber transferase (a
cis-prenyltransferase). Rubber transferase uses allylic pyrophosphate to initiate the rubber molecule and isopentenyl pyrophosphate (IPP) to form the polymer. Rubber biosynthesis also requires a divalent metal cation. Understanding how molecular weight is regulated is important because high molecular weight is required for high quality rubber. We characterized the in vitro effects of Mg
2+ on the biosynthetic rate of rubber produced by an alternative natural rubber crop,
Parthenium argentatum (guayule). The affinity of the rubber transferase from
P. argentatum for IPP
·
Mg was shown to depend on the Mg
2+ concentration in a similar fashion to the
H. brasiliensis rubber transferase, although to a less extreme degree. Also, in vitro Mg
2+ concentration significantly affects rubber molecular weight of both species, but molecular weight is less sensitive to Mg
2+ concentration in
P. argentatum than in
H. brasiliensis.</description><subject>alkyl (aryl) transferases</subject><subject>Asteraceae</subject><subject>Asteraceae - metabolism</subject><subject>Biological and medical sciences</subject><subject>biosynthesis</subject><subject>cis-prenyl transferase</subject><subject>cis-Prenyltransferase</subject><subject>Compositae</subject><subject>enzyme activity</subject><subject>Euphorbiaceae</subject><subject>farnesyl pyrophosphate</subject><subject>FPP</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hevea brasiliensis</subject><subject>Hevea brasiliensis Muell. Arg</subject><subject>IPP</subject><subject>isopentenyl pyrophosphate</subject><subject>Kinetics</subject><subject>Magnesium</subject><subject>Magnesium - pharmacology</subject><subject>Metabolism</subject><subject>Metabolism. Physicochemical requirements</subject><subject>metal ions</subject><subject>Molecular Weight</subject><subject>Parthenium argentatum</subject><subject>Plant physiology and development</subject><subject>Regulation</subject><subject>Rubber</subject><subject>rubber prenyltransferase</subject><subject>Rubber transferase</subject><issn>0031-9422</issn><issn>1873-3700</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkM1u1DAURi0EotPCK9BsYJdwbcdxZllVpVQqAgnK1nKc6xmPkniwnUp5exzNiC5Z-Vo63_05hFxTqCjQ5vOhOu6X5M0ex4oBNBXUFVB4RTa0lbzkEuA12QBwWm5rxi7IZYwHABCiad6SC9rIFrYgNuT3N72bMLp5LJyfioC7edBpLb0t3FQ8uxR8Eeauw1B0zsdlSvvMx6Jbih865M-0hnXY4ZR0yuV90Ms78sbqIeL783tFnr7c_br9Wj5-v3-4vXksTc14KgVw0GClZoIJYXRbW2k5Sqxt00vserllDRWCa-zaLq9st2CFaRkIK7MIfkU-nfoeg_8zY0xqdNHgMOgJ_RxV00pJOYcMyhNogo8xoFXH4EYdFkVBrUrVQf1TqlalCmqVlebkh_OIuRuxf8mdHWbg4xnQ0ejBBj0ZF1-4FmTdcp656xNntVd6FzLz9JPlI4BSqIVYR92cCMzKnh0GFY3DyWDvApqkeu_-u-5ftCWi4Q</recordid><startdate>20060801</startdate><enddate>20060801</enddate><creator>da Costa, Bernardo M.T.</creator><creator>Keasling, Jay D.</creator><creator>McMahan, Colleen M.</creator><creator>Cornish, Katrina</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20060801</creationdate><title>Magnesium ion regulation of in vitro rubber biosynthesis by Parthenium argentatum Gray</title><author>da Costa, Bernardo M.T. ; Keasling, Jay D. ; McMahan, Colleen M. ; Cornish, Katrina</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c423t-5030a0f7a25255ca84f7f3e7e4f6d7ebd79261553aeb8b809f90f5c8205f71013</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>alkyl (aryl) transferases</topic><topic>Asteraceae</topic><topic>Asteraceae - metabolism</topic><topic>Biological and medical sciences</topic><topic>biosynthesis</topic><topic>cis-prenyl transferase</topic><topic>cis-Prenyltransferase</topic><topic>Compositae</topic><topic>enzyme activity</topic><topic>Euphorbiaceae</topic><topic>farnesyl pyrophosphate</topic><topic>FPP</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hevea brasiliensis</topic><topic>Hevea brasiliensis Muell. Arg</topic><topic>IPP</topic><topic>isopentenyl pyrophosphate</topic><topic>Kinetics</topic><topic>Magnesium</topic><topic>Magnesium - pharmacology</topic><topic>Metabolism</topic><topic>Metabolism. Physicochemical requirements</topic><topic>metal ions</topic><topic>Molecular Weight</topic><topic>Parthenium argentatum</topic><topic>Plant physiology and development</topic><topic>Regulation</topic><topic>Rubber</topic><topic>rubber prenyltransferase</topic><topic>Rubber transferase</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>da Costa, Bernardo M.T.</creatorcontrib><creatorcontrib>Keasling, Jay D.</creatorcontrib><creatorcontrib>McMahan, Colleen M.</creatorcontrib><creatorcontrib>Cornish, Katrina</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Phytochemistry (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>da Costa, Bernardo M.T.</au><au>Keasling, Jay D.</au><au>McMahan, Colleen M.</au><au>Cornish, Katrina</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Magnesium ion regulation of in vitro rubber biosynthesis by Parthenium argentatum Gray</atitle><jtitle>Phytochemistry (Oxford)</jtitle><addtitle>Phytochemistry</addtitle><date>2006-08-01</date><risdate>2006</risdate><volume>67</volume><issue>15</issue><spage>1621</spage><epage>1628</epage><pages>1621-1628</pages><issn>0031-9422</issn><eissn>1873-3700</eissn><abstract>The rate of rubber biosynthesis and the molecular weight of the rubber produced are both strongly affected by [Mg
2+] in
Parthenium argentatum.
Natural rubber is produced by a rubber transferase (a
cis-prenyltransferase). Rubber transferase uses allylic pyrophosphate to initiate the rubber molecule and isopentenyl pyrophosphate (IPP) to form the polymer. Rubber biosynthesis also requires a divalent metal cation. Understanding how molecular weight is regulated is important because high molecular weight is required for high quality rubber. We characterized the in vitro effects of Mg
2+ on the biosynthetic rate of rubber produced by an alternative natural rubber crop,
Parthenium argentatum (guayule). The affinity of the rubber transferase from
P. argentatum for IPP
·
Mg was shown to depend on the Mg
2+ concentration in a similar fashion to the
H. brasiliensis rubber transferase, although to a less extreme degree. Also, in vitro Mg
2+ concentration significantly affects rubber molecular weight of both species, but molecular weight is less sensitive to Mg
2+ concentration in
P. argentatum than in
H. brasiliensis.</abstract><cop>Amsterdam</cop><pub>Elsevier Ltd</pub><pmid>16780905</pmid><doi>10.1016/j.phytochem.2006.04.010</doi><tpages>8</tpages></addata></record> |
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| subjects | alkyl (aryl) transferases Asteraceae Asteraceae - metabolism Biological and medical sciences biosynthesis cis-prenyl transferase cis-Prenyltransferase Compositae enzyme activity Euphorbiaceae farnesyl pyrophosphate FPP Fundamental and applied biological sciences. Psychology Hevea brasiliensis Hevea brasiliensis Muell. Arg IPP isopentenyl pyrophosphate Kinetics Magnesium Magnesium - pharmacology Metabolism Metabolism. Physicochemical requirements metal ions Molecular Weight Parthenium argentatum Plant physiology and development Regulation Rubber rubber prenyltransferase Rubber transferase |
| title | Magnesium ion regulation of in vitro rubber biosynthesis by Parthenium argentatum Gray |
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