Direct Role for Dual Oxidase in Drosophila Gut Immunity
Because the mucosal epithelia are in constant contact with large numbers of microorganisms, these surfaces must be armed with efficient microbial control systems. Here, we show that the Drosophila nicotinamide adenine dinucleotide phosphate (NADPH) oxidase enzyme, dual oxidase (dDuox), is indispensa...
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| Veröffentlicht in: | Science (American Association for the Advancement of Science) 2005-11, Vol.310 (5749), p.847-850 |
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| creator | Ha, Eun-Mi Oh, Chun-Taek Bae, Yun Soo Lee, Won-Jae |
| description | Because the mucosal epithelia are in constant contact with large numbers of microorganisms, these surfaces must be armed with efficient microbial control systems. Here, we show that the Drosophila nicotinamide adenine dinucleotide phosphate (NADPH) oxidase enzyme, dual oxidase (dDuox), is indispensable for gut antimicrobial activities. Adult flies in which dDuox expression is silenced showed a marked increase in mortality rate even after a minor infection through ingestion of microbe-contaminated food. This could be restored by the specific reintroduction of dDuox, demonstrating that this oxidase generates a unique epithelial oxidative burst that limits microbial proliferation in the gut. Thus, oxidant-mediated antimicrobial responses are not restricted to the phagocytes, but rather are used more broadly, including in mucosal barrier epithelia. |
| doi_str_mv | 10.1126/science.1117311 |
| format | Article |
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Here, we show that the Drosophila nicotinamide adenine dinucleotide phosphate (NADPH) oxidase enzyme, dual oxidase (dDuox), is indispensable for gut antimicrobial activities. Adult flies in which dDuox expression is silenced showed a marked increase in mortality rate even after a minor infection through ingestion of microbe-contaminated food. This could be restored by the specific reintroduction of dDuox, demonstrating that this oxidase generates a unique epithelial oxidative burst that limits microbial proliferation in the gut. Thus, oxidant-mediated antimicrobial responses are not restricted to the phagocytes, but rather are used more broadly, including in mucosal barrier epithelia.</description><identifier>ISSN: 0036-8075</identifier><identifier>EISSN: 1095-9203</identifier><identifier>DOI: 10.1126/science.1117311</identifier><identifier>PMID: 16272120</identifier><identifier>CODEN: SCIEAS</identifier><language>eng</language><publisher>Washington, DC: American Association for the Advancement of Science</publisher><subject>Animals ; Animals, Genetically Modified ; Attrition (Research Studies) ; Bacteria - growth & development ; Bacteria - immunology ; bacterial contamination ; bacterial infections ; Biochemistry. Physiology. Immunology ; Biological and medical sciences ; Catalase - genetics ; Catalase - metabolism ; Chlorides - metabolism ; Colony Count, Microbial ; Digestive System - enzymology ; Digestive System - immunology ; Digestive System - microbiology ; digestive tract ; disease resistance ; Drosophila ; Drosophila - enzymology ; Drosophila - immunology ; Drosophila - microbiology ; Drosophila melanogaster ; dual oxidase ; enzyme activity ; Enzymes ; Eukaryotes ; foods ; Fundamental and applied biological sciences. Psychology ; Immune system ; Immunity ; Immunity, Innate ; In Vitro Techniques ; Infections ; ingestion ; Innate immunity ; Insecta ; Insects ; Intestinal Mucosa - enzymology ; Intestinal Mucosa - immunology ; Intestinal Mucosa - microbiology ; Intestines ; Invertebrates ; Materials ; Microbiology ; Microorganisms ; Mortality Rate ; NADPH oxidase ; NADPH Oxidases - genetics ; NADPH Oxidases - metabolism ; Oxidases ; oxidoreductases ; Phagocytes ; Physiology. 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Here, we show that the Drosophila nicotinamide adenine dinucleotide phosphate (NADPH) oxidase enzyme, dual oxidase (dDuox), is indispensable for gut antimicrobial activities. Adult flies in which dDuox expression is silenced showed a marked increase in mortality rate even after a minor infection through ingestion of microbe-contaminated food. This could be restored by the specific reintroduction of dDuox, demonstrating that this oxidase generates a unique epithelial oxidative burst that limits microbial proliferation in the gut. Thus, oxidant-mediated antimicrobial responses are not restricted to the phagocytes, but rather are used more broadly, including in mucosal barrier epithelia.</description><subject>Animals</subject><subject>Animals, Genetically Modified</subject><subject>Attrition (Research Studies)</subject><subject>Bacteria - growth & development</subject><subject>Bacteria - immunology</subject><subject>bacterial contamination</subject><subject>bacterial infections</subject><subject>Biochemistry. Physiology. 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Physiology. Immunology</topic><topic>Biological and medical sciences</topic><topic>Catalase - genetics</topic><topic>Catalase - metabolism</topic><topic>Chlorides - metabolism</topic><topic>Colony Count, Microbial</topic><topic>Digestive System - enzymology</topic><topic>Digestive System - immunology</topic><topic>Digestive System - microbiology</topic><topic>digestive tract</topic><topic>disease resistance</topic><topic>Drosophila</topic><topic>Drosophila - enzymology</topic><topic>Drosophila - immunology</topic><topic>Drosophila - microbiology</topic><topic>Drosophila melanogaster</topic><topic>dual oxidase</topic><topic>enzyme activity</topic><topic>Enzymes</topic><topic>Eukaryotes</topic><topic>foods</topic><topic>Fundamental and applied biological sciences. 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Role for Dual Oxidase in Drosophila Gut Immunity</atitle><jtitle>Science (American Association for the Advancement of Science)</jtitle><addtitle>Science</addtitle><date>2005-11-04</date><risdate>2005</risdate><volume>310</volume><issue>5749</issue><spage>847</spage><epage>850</epage><pages>847-850</pages><issn>0036-8075</issn><eissn>1095-9203</eissn><coden>SCIEAS</coden><abstract>Because the mucosal epithelia are in constant contact with large numbers of microorganisms, these surfaces must be armed with efficient microbial control systems. Here, we show that the Drosophila nicotinamide adenine dinucleotide phosphate (NADPH) oxidase enzyme, dual oxidase (dDuox), is indispensable for gut antimicrobial activities. Adult flies in which dDuox expression is silenced showed a marked increase in mortality rate even after a minor infection through ingestion of microbe-contaminated food. This could be restored by the specific reintroduction of dDuox, demonstrating that this oxidase generates a unique epithelial oxidative burst that limits microbial proliferation in the gut. Thus, oxidant-mediated antimicrobial responses are not restricted to the phagocytes, but rather are used more broadly, including in mucosal barrier epithelia.</abstract><cop>Washington, DC</cop><pub>American Association for the Advancement of Science</pub><pmid>16272120</pmid><doi>10.1126/science.1117311</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0036-8075 |
| ispartof | Science (American Association for the Advancement of Science), 2005-11, Vol.310 (5749), p.847-850 |
| issn | 0036-8075 1095-9203 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_68770599 |
| source | American Association for the Advancement of Science; Jstor Complete Legacy; MEDLINE |
| subjects | Animals Animals, Genetically Modified Attrition (Research Studies) Bacteria - growth & development Bacteria - immunology bacterial contamination bacterial infections Biochemistry. Physiology. Immunology Biological and medical sciences Catalase - genetics Catalase - metabolism Chlorides - metabolism Colony Count, Microbial Digestive System - enzymology Digestive System - immunology Digestive System - microbiology digestive tract disease resistance Drosophila Drosophila - enzymology Drosophila - immunology Drosophila - microbiology Drosophila melanogaster dual oxidase enzyme activity Enzymes Eukaryotes foods Fundamental and applied biological sciences. Psychology Immune system Immunity Immunity, Innate In Vitro Techniques Infections ingestion Innate immunity Insecta Insects Intestinal Mucosa - enzymology Intestinal Mucosa - immunology Intestinal Mucosa - microbiology Intestines Invertebrates Materials Microbiology Microorganisms Mortality Rate NADPH oxidase NADPH Oxidases - genetics NADPH Oxidases - metabolism Oxidases oxidoreductases Phagocytes Physiology. Development Reactive Oxygen Species - metabolism Respiratory Burst RNA Interference Saccharomyces cerevisiae - growth & development Saccharomyces cerevisiae - immunology Superoxides Superoxides - metabolism T lymphocytes |
| title | Direct Role for Dual Oxidase in Drosophila Gut Immunity |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-21T19%3A47%3A18IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Direct%20Role%20for%20Dual%20Oxidase%20in%20Drosophila%20Gut%20Immunity&rft.jtitle=Science%20(American%20Association%20for%20the%20Advancement%20of%20Science)&rft.au=Ha,%20Eun-Mi&rft.date=2005-11-04&rft.volume=310&rft.issue=5749&rft.spage=847&rft.epage=850&rft.pages=847-850&rft.issn=0036-8075&rft.eissn=1095-9203&rft.coden=SCIEAS&rft_id=info:doi/10.1126/science.1117311&rft_dat=%3Cgale_proqu%3EA138859342%3C/gale_proqu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=213613103&rft_id=info:pmid/16272120&rft_galeid=A138859342&rft_jstor_id=3842764&rfr_iscdi=true |