Chain Conformations of Poly(γ-benzyl-L-glutamate) Pre and Post an Electrospinning Process
In order to reveal mechanisms for the electrospinning of proteins, this study focuses on the polymer chain conformation, which is considered to be a critical factor for successful electrospinning. Poly(γ‐benzyl‐L‐glutamate) (PBLG) is employed and the relationships between the chain conformations of...
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Veröffentlicht in: | Macromolecular bioscience 2006-07, Vol.6 (7), p.487-495 |
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description | In order to reveal mechanisms for the electrospinning of proteins, this study focuses on the polymer chain conformation, which is considered to be a critical factor for successful electrospinning. Poly(γ‐benzyl‐L‐glutamate) (PBLG) is employed and the relationships between the chain conformations of the pre‐spun PBLG molecules and the morphologies of the post‐spun PBLG fibers are investigated. By combining viscosity measurements, and circular dichroism and FT‐IR spectroscopies, chain conformations of the pre‐ and post‐spun PBLG are characterized. The chain conformations of the pre‐spun PBLG changes from an α‐helix to a random coil upon changing the solvent ratios of dichloromethane (CH2Cl2) and trifluoroacetic acid (CF3COOH) from 100:0 to 0:100. In an α‐helix conformation, the morphology of PBLG fibers is relatively thick, while that of the random coil is thin and homogenous. The mean fiber diameters decrease when the chain conformations change from an α‐helix to a random coil. FT‐IR spectroscopy and wide‐angle X‐ray diffraction measurements reveal that electrospinning predominantly induces an α‐helical conformation in post‐spun PBLG fibers, and more highly crystallized fibers are generated as the α‐helical content in the pre‐spun solution increases.
SEM images of the electrospun PBLG fibers ($\overline {DP}$ = 1 200) at DCM/TFA ratios of a) 100:0, b) 90:10, c) 80:20, and d) 0:100 (magnification: ×1 000, scale bars: 20 µm). |
doi_str_mv | 10.1002/mabi.200600066 |
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SEM images of the electrospun PBLG fibers ($\overline {DP}$ = 1 200) at DCM/TFA ratios of a) 100:0, b) 90:10, c) 80:20, and d) 0:100 (magnification: ×1 000, scale bars: 20 µm).</description><identifier>ISSN: 1616-5187</identifier><identifier>EISSN: 1616-5195</identifier><identifier>DOI: 10.1002/mabi.200600066</identifier><identifier>PMID: 16921536</identifier><language>eng</language><publisher>Weinheim: WILEY-VCH Verlag</publisher><subject>Aminoacid polymers ; Applied sciences ; conformational analysis ; Electrochemistry ; electrospinning ; Exact sciences and technology ; Machinery and processing ; Molecular Conformation ; Molecular Weight ; non-woven fabric ; Physicochemistry of polymers ; Plastics ; poly(amino acid)s ; poly(γ-benzyl-L-glutamate) ; Polyglutamic Acid - analogs & derivatives ; Polyglutamic Acid - chemistry ; Polymer industry, paints, wood ; Spectroscopy, Fourier Transform Infrared ; Spinning ; Synthetic biopolymers ; Technology of polymers</subject><ispartof>Macromolecular bioscience, 2006-07, Vol.6 (7), p.487-495</ispartof><rights>Copyright © 2006 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim</rights><rights>2006 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5086-da08be3bc7be2fd50c65edee15629537dc2bca259b9520961121d812577910f43</citedby><cites>FETCH-LOGICAL-c5086-da08be3bc7be2fd50c65edee15629537dc2bca259b9520961121d812577910f43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fmabi.200600066$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fmabi.200600066$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17948992$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16921536$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Minato, Ken-Ichi</creatorcontrib><creatorcontrib>Ohkawa, Kousaku</creatorcontrib><creatorcontrib>Yamamoto, Hiroyuki</creatorcontrib><title>Chain Conformations of Poly(γ-benzyl-L-glutamate) Pre and Post an Electrospinning Process</title><title>Macromolecular bioscience</title><addtitle>Macromol. Biosci</addtitle><description>In order to reveal mechanisms for the electrospinning of proteins, this study focuses on the polymer chain conformation, which is considered to be a critical factor for successful electrospinning. Poly(γ‐benzyl‐L‐glutamate) (PBLG) is employed and the relationships between the chain conformations of the pre‐spun PBLG molecules and the morphologies of the post‐spun PBLG fibers are investigated. By combining viscosity measurements, and circular dichroism and FT‐IR spectroscopies, chain conformations of the pre‐ and post‐spun PBLG are characterized. The chain conformations of the pre‐spun PBLG changes from an α‐helix to a random coil upon changing the solvent ratios of dichloromethane (CH2Cl2) and trifluoroacetic acid (CF3COOH) from 100:0 to 0:100. In an α‐helix conformation, the morphology of PBLG fibers is relatively thick, while that of the random coil is thin and homogenous. The mean fiber diameters decrease when the chain conformations change from an α‐helix to a random coil. FT‐IR spectroscopy and wide‐angle X‐ray diffraction measurements reveal that electrospinning predominantly induces an α‐helical conformation in post‐spun PBLG fibers, and more highly crystallized fibers are generated as the α‐helical content in the pre‐spun solution increases.
SEM images of the electrospun PBLG fibers ($\overline {DP}$ = 1 200) at DCM/TFA ratios of a) 100:0, b) 90:10, c) 80:20, and d) 0:100 (magnification: ×1 000, scale bars: 20 µm).</description><subject>Aminoacid polymers</subject><subject>Applied sciences</subject><subject>conformational analysis</subject><subject>Electrochemistry</subject><subject>electrospinning</subject><subject>Exact sciences and technology</subject><subject>Machinery and processing</subject><subject>Molecular Conformation</subject><subject>Molecular Weight</subject><subject>non-woven fabric</subject><subject>Physicochemistry of polymers</subject><subject>Plastics</subject><subject>poly(amino acid)s</subject><subject>poly(γ-benzyl-L-glutamate)</subject><subject>Polyglutamic Acid - analogs & derivatives</subject><subject>Polyglutamic Acid - chemistry</subject><subject>Polymer industry, paints, wood</subject><subject>Spectroscopy, Fourier Transform Infrared</subject><subject>Spinning</subject><subject>Synthetic biopolymers</subject><subject>Technology of polymers</subject><issn>1616-5187</issn><issn>1616-5195</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0M9PFDEUB_CGaATRK0cyF40eZmk7219HXBFIVuSgSLw0nc4bKHbatZ2Nrv-W_4d_kyW7WbxxaNqkn_f6-kXogOAJwZgeDaZ1E4oxx2XxHbRHOOE1I4o92Z6l2EXPc77DmAip6DO0S7iihDV8D32b3RoXqlkMfUyDGV0MuYp9dRn96s3fP3UL4ffK1_P6xi9HUwC8rS4TVCZ0xeSxHKoTD3ZMMS9cCC7clPtoIecX6GlvfIaXm30ffflw8nl2Vs8_nZ7Pjue1ZVjyujNYttC0VrRA-45hyxl0AIRxqlgjOktbayhTrWIUK04IJZ0klAmhCO6nzT56ve67SPHHEvKoB5cteG8CxGXWXAquplg-ComalokaXuBkDW35VU7Q60Vyg0krTbC-j13fx663sZeCw03nZTtA98A3ORfwagNMtsb3yQTr8oMTaiqVosWptfvpPKweeVZ_PH53_v8Q9brW5RF-bWtN-q65aATTXy9O9ftrIcnV2bUWzT-f16qq</recordid><startdate>20060714</startdate><enddate>20060714</enddate><creator>Minato, Ken-Ichi</creator><creator>Ohkawa, Kousaku</creator><creator>Yamamoto, Hiroyuki</creator><general>WILEY-VCH Verlag</general><general>WILEY‐VCH Verlag</general><general>Wiley-VCH</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20060714</creationdate><title>Chain Conformations of Poly(γ-benzyl-L-glutamate) Pre and Post an Electrospinning Process</title><author>Minato, Ken-Ichi ; Ohkawa, Kousaku ; Yamamoto, Hiroyuki</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5086-da08be3bc7be2fd50c65edee15629537dc2bca259b9520961121d812577910f43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Aminoacid polymers</topic><topic>Applied sciences</topic><topic>conformational analysis</topic><topic>Electrochemistry</topic><topic>electrospinning</topic><topic>Exact sciences and technology</topic><topic>Machinery and processing</topic><topic>Molecular Conformation</topic><topic>Molecular Weight</topic><topic>non-woven fabric</topic><topic>Physicochemistry of polymers</topic><topic>Plastics</topic><topic>poly(amino acid)s</topic><topic>poly(γ-benzyl-L-glutamate)</topic><topic>Polyglutamic Acid - analogs & derivatives</topic><topic>Polyglutamic Acid - chemistry</topic><topic>Polymer industry, paints, wood</topic><topic>Spectroscopy, Fourier Transform Infrared</topic><topic>Spinning</topic><topic>Synthetic biopolymers</topic><topic>Technology of polymers</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Minato, Ken-Ichi</creatorcontrib><creatorcontrib>Ohkawa, Kousaku</creatorcontrib><creatorcontrib>Yamamoto, Hiroyuki</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Macromolecular bioscience</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Minato, Ken-Ichi</au><au>Ohkawa, Kousaku</au><au>Yamamoto, Hiroyuki</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Chain Conformations of Poly(γ-benzyl-L-glutamate) Pre and Post an Electrospinning Process</atitle><jtitle>Macromolecular bioscience</jtitle><addtitle>Macromol. Biosci</addtitle><date>2006-07-14</date><risdate>2006</risdate><volume>6</volume><issue>7</issue><spage>487</spage><epage>495</epage><pages>487-495</pages><issn>1616-5187</issn><eissn>1616-5195</eissn><abstract>In order to reveal mechanisms for the electrospinning of proteins, this study focuses on the polymer chain conformation, which is considered to be a critical factor for successful electrospinning. Poly(γ‐benzyl‐L‐glutamate) (PBLG) is employed and the relationships between the chain conformations of the pre‐spun PBLG molecules and the morphologies of the post‐spun PBLG fibers are investigated. By combining viscosity measurements, and circular dichroism and FT‐IR spectroscopies, chain conformations of the pre‐ and post‐spun PBLG are characterized. The chain conformations of the pre‐spun PBLG changes from an α‐helix to a random coil upon changing the solvent ratios of dichloromethane (CH2Cl2) and trifluoroacetic acid (CF3COOH) from 100:0 to 0:100. In an α‐helix conformation, the morphology of PBLG fibers is relatively thick, while that of the random coil is thin and homogenous. The mean fiber diameters decrease when the chain conformations change from an α‐helix to a random coil. FT‐IR spectroscopy and wide‐angle X‐ray diffraction measurements reveal that electrospinning predominantly induces an α‐helical conformation in post‐spun PBLG fibers, and more highly crystallized fibers are generated as the α‐helical content in the pre‐spun solution increases.
SEM images of the electrospun PBLG fibers ($\overline {DP}$ = 1 200) at DCM/TFA ratios of a) 100:0, b) 90:10, c) 80:20, and d) 0:100 (magnification: ×1 000, scale bars: 20 µm).</abstract><cop>Weinheim</cop><pub>WILEY-VCH Verlag</pub><pmid>16921536</pmid><doi>10.1002/mabi.200600066</doi><tpages>9</tpages></addata></record> |
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subjects | Aminoacid polymers Applied sciences conformational analysis Electrochemistry electrospinning Exact sciences and technology Machinery and processing Molecular Conformation Molecular Weight non-woven fabric Physicochemistry of polymers Plastics poly(amino acid)s poly(γ-benzyl-L-glutamate) Polyglutamic Acid - analogs & derivatives Polyglutamic Acid - chemistry Polymer industry, paints, wood Spectroscopy, Fourier Transform Infrared Spinning Synthetic biopolymers Technology of polymers |
title | Chain Conformations of Poly(γ-benzyl-L-glutamate) Pre and Post an Electrospinning Process |
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