Factor I-Mediated Processing of Complement Fragments on HIV Immune Complexes Targets HIV to CR2-Expressing B Cells and Facilitates B Cell-Mediated Transmission of Opsonized HIV to T Cells
Our study demonstrates that binding of complement-opsonized HIV to complement receptor type 1 on human erythrocytes (E) via C3b fragments is followed by a rapid normal human serum-mediated detachment of HIV from E. The release was dependent on the presence of factor I indicating a conversion of C3b...
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| Veröffentlicht in: | Journal of Immunology 2006-09, Vol.177 (5), p.3469-3476 |
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| creator | Banki, Zoltan Wilflingseder, Doris Ammann, Christoph G Pruenster, Monika Mullauer, Brigitte Hollander, Karoline Meyer, Martina Sprinzl, Georg M van Lunzen, Jan Stellbrink, Hans-Jurgen Dierich, Manfred P Stoiber, Heribert |
| description | Our study demonstrates that binding of complement-opsonized HIV to complement receptor type 1 on human erythrocytes (E) via C3b fragments is followed by a rapid normal human serum-mediated detachment of HIV from E. The release was dependent on the presence of factor I indicating a conversion of C3b fragments to iC3b and C3d on the viral surface. This in turn resulted in an efficient binding of opsonized HIV to CR2-expressing B cells, thus facilitating B cell-mediated transmission of HIV to T cells. These data provide a new dynamic view of complement opsonization of HIV, suggesting that association of virus with E might be a transient phenomenon and the factor I-mediated processing of C3b to iC3b and C3d on HIV targets the virus to complement receptor type 2-expressing cells. Thus, factor I in concert with CR1 on E and factor H in serum due to their cofactor activity are likely to be important contributors for the generation of C3d-opsonized infectious HIV reservoirs on follicular dendritic cells and/or B cells in HIV-infected individuals. |
| doi_str_mv | 10.4049/jimmunol.177.5.3469 |
| format | Article |
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| subjects | B-Lymphocytes - immunology B-Lymphocytes - metabolism B-Lymphocytes - virology Complement System Proteins - chemistry Complement System Proteins - immunology Complement System Proteins - metabolism Fibrinogen - metabolism HIV - immunology HIV Infections - immunology HIV Infections - virology Human immunodeficiency virus Humans Kinetics Receptors, Complement 3d - immunology Serum T-Lymphocytes - immunology T-Lymphocytes - virology |
| title | Factor I-Mediated Processing of Complement Fragments on HIV Immune Complexes Targets HIV to CR2-Expressing B Cells and Facilitates B Cell-Mediated Transmission of Opsonized HIV to T Cells |
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