Phosphorylation of the Brn-3a transcription factor is modulated during differentiation and regulates its functional activity
Brn-3a is a transcription factor expressed in a subset of neurons of the peripheral nervous system. Its role encompasses the activation of genes involved in neuronal differentiation and survival. While a lot of data have been produced on Brn-3a target promoters, very little is known about the upstre...
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| Veröffentlicht in: | Brain research. Molecular brain research. 2005-11, Vol.141 (1), p.10-18 |
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| container_title | Brain research. Molecular brain research. |
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| creator | Calissano, Mattia Faulkes, David Latchman, David S. |
| description | Brn-3a is a transcription factor expressed in a subset of neurons of the peripheral nervous system. Its role encompasses the activation of genes involved in neuronal differentiation and survival. While a lot of data have been produced on Brn-3a target promoters, very little is known about the upstream regulatory signals that mediate its activation in response to differentiation. In this work, we describe for the first time that Brn-3a is phosphorylated in IMR-32 neuroblastoma cells in response to differentiation induced by retinoic acid treatment and that its post-translational modification is potentially mediated by the activation of the MAPK/ERK pathway. Furthermore, we show that the mutation of a putative phosphorylated amino acid strongly reduces the ability of Brn-3a to mediate the differentiation of IMR-32 cells. |
| doi_str_mv | 10.1016/j.molbrainres.2005.07.013 |
| format | Article |
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Its role encompasses the activation of genes involved in neuronal differentiation and survival. While a lot of data have been produced on Brn-3a target promoters, very little is known about the upstream regulatory signals that mediate its activation in response to differentiation. In this work, we describe for the first time that Brn-3a is phosphorylated in IMR-32 neuroblastoma cells in response to differentiation induced by retinoic acid treatment and that its post-translational modification is potentially mediated by the activation of the MAPK/ERK pathway. 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Molecular brain research.</title><addtitle>Brain Res Mol Brain Res</addtitle><description>Brn-3a is a transcription factor expressed in a subset of neurons of the peripheral nervous system. Its role encompasses the activation of genes involved in neuronal differentiation and survival. While a lot of data have been produced on Brn-3a target promoters, very little is known about the upstream regulatory signals that mediate its activation in response to differentiation. In this work, we describe for the first time that Brn-3a is phosphorylated in IMR-32 neuroblastoma cells in response to differentiation induced by retinoic acid treatment and that its post-translational modification is potentially mediated by the activation of the MAPK/ERK pathway. 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Psychology</subject><subject>Gene Expression Regulation, Developmental</subject><subject>Genes, Reporter</subject><subject>Humans</subject><subject>MAP Kinase Signaling System - physiology</subject><subject>Mice</subject><subject>Molecular Sequence Data</subject><subject>Neurons - cytology</subject><subject>Neurons - drug effects</subject><subject>Neurons - physiology</subject><subject>Phosphorylation</subject><subject>Retinoic acid</subject><subject>Sequence Alignment</subject><subject>Transcription factor</subject><subject>Transcription Factor Brn-3A - metabolism</subject><subject>Tretinoin - pharmacology</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0169-328X</issn><issn>1872-6941</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc2KFDEUhYMoTjv6ChIXuqsyf5VULbXxDwZ0oeAupFI302mqkjZJDTT48KanG8adrgK53zkJ90PoFSUtJVS-3bdLnMdkfEiQW0ZI1xLVEsofoQ3tFWvkIOhjtKns0HDW_7xCz3LeE0JoT-lTdEUlZZITukG_v-1iPuxiOs6m-BhwdLjsAL9PoeEGl2RCtskf7mfO2BIT9hkvcVprACY8rcmHWzx55yBBKP5cY8KEE9zeQxn7krFbgz2NzIxrjb_z5fgcPXFmzvDicl6jHx8_fN9-bm6-fvqyfXfTWMFlaSTruRyMYdIKQYd6OXIrO2GZHccBOkUZKCkGJwZmnaDAjBFESGU6Yut2-DV6c-49pPhrhVz04rOFeTYB4pq17JWUZBD_BKkSHR86XsHhDNoUc07g9CH5xaSjpkSfHOm9_suRPjnSROnqqGZfXh5ZxwWmh-RFSgVeXwCTrZlddWB9fuAU67qOq8ptzxzU3d15SDpbD8HC5BPYoqfo_-M7fwBznbgf</recordid><startdate>20051118</startdate><enddate>20051118</enddate><creator>Calissano, Mattia</creator><creator>Faulkes, David</creator><creator>Latchman, David S.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20051118</creationdate><title>Phosphorylation of the Brn-3a transcription factor is modulated during differentiation and regulates its functional activity</title><author>Calissano, Mattia ; Faulkes, David ; Latchman, David S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c436t-628369aa26c4419c43b3c654c2cbb9e5712e7649f492cf41e2aa40467a50c2003</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Brn-3a</topic><topic>Cell Differentiation - physiology</topic><topic>Cell Line, Tumor</topic><topic>Differentiation</topic><topic>Enzyme Activation</topic><topic>Fundamental and applied biological sciences. 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Molecular brain research.</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Calissano, Mattia</au><au>Faulkes, David</au><au>Latchman, David S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Phosphorylation of the Brn-3a transcription factor is modulated during differentiation and regulates its functional activity</atitle><jtitle>Brain research. Molecular brain research.</jtitle><addtitle>Brain Res Mol Brain Res</addtitle><date>2005-11-18</date><risdate>2005</risdate><volume>141</volume><issue>1</issue><spage>10</spage><epage>18</epage><pages>10-18</pages><issn>0169-328X</issn><eissn>1872-6941</eissn><abstract>Brn-3a is a transcription factor expressed in a subset of neurons of the peripheral nervous system. Its role encompasses the activation of genes involved in neuronal differentiation and survival. 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| ispartof | Brain research. Molecular brain research., 2005-11, Vol.141 (1), p.10-18 |
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| subjects | Amino Acid Sequence Animals Biological and medical sciences Brn-3a Cell Differentiation - physiology Cell Line, Tumor Differentiation Enzyme Activation Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Developmental Genes, Reporter Humans MAP Kinase Signaling System - physiology Mice Molecular Sequence Data Neurons - cytology Neurons - drug effects Neurons - physiology Phosphorylation Retinoic acid Sequence Alignment Transcription factor Transcription Factor Brn-3A - metabolism Tretinoin - pharmacology Vertebrates: nervous system and sense organs |
| title | Phosphorylation of the Brn-3a transcription factor is modulated during differentiation and regulates its functional activity |
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