Molecular Architecture of Axonemes Revealed by Cryoelectron Tomography

Eukaryotic flagella and cilia are built on a 9 + 2 array of microtubules plus >250 accessory proteins, forming a biological machine called the axoneme. Here we describe the three-dimensional structure of rapidly frozen axonemes from Chlamydomonas and sea urchin sperm, using cryoelectron tomograph...

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Veröffentlicht in:	Science (American Association for the Advancement of Science) 2006-08, Vol.313 (5789), p.944-948
Hauptverfasser:	Nicastro, Daniela, Schwartz, Cindi, Pierson, Jason, Gaudette, Richard, Porter, Mary E, McIntosh, J. Richard
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Atoms Biological and medical sciences Carrier Proteins - chemistry Carrier Proteins - ultrastructure Cell motility Cell structures and functions Chlamydomonas Chlamydomonas reinhardtii - ultrastructure Cryoelectron Microscopy Dyneins - chemistry Dyneins - physiology Dyneins - ultrastructure Echinoidea Electrons Enzymes Eukaryotes Flagella Flagella - chemistry Flagella - physiology Flagella - ultrastructure Freezing Fundamental and applied biological sciences. Psychology Image Processing, Computer-Assisted Imaging, Three-Dimensional Male Materials Microtubule-Associated Proteins Microtubules Microtubules - chemistry Microtubules - physiology Microtubules - ultrastructure Miscellaneous Models, Biological Molecular and cellular biology Molecular Motor Proteins - chemistry Molecular Motor Proteins - ultrastructure Molecular structure Periodicity Protein Structure, Tertiary Proteins Sea Urchins Sperm Tail - chemistry Sperm Tail - physiology Sperm Tail - ultrastructure Spermatozoa Tomography
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