Molecular Architecture of Axonemes Revealed by Cryoelectron Tomography
Eukaryotic flagella and cilia are built on a 9 + 2 array of microtubules plus >250 accessory proteins, forming a biological machine called the axoneme. Here we describe the three-dimensional structure of rapidly frozen axonemes from Chlamydomonas and sea urchin sperm, using cryoelectron tomograph...
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| Veröffentlicht in: | Science (American Association for the Advancement of Science) 2006-08, Vol.313 (5789), p.944-948 |
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| creator | Nicastro, Daniela Schwartz, Cindi Pierson, Jason Gaudette, Richard Porter, Mary E McIntosh, J. Richard |
| description | Eukaryotic flagella and cilia are built on a 9 + 2 array of microtubules plus >250 accessory proteins, forming a biological machine called the axoneme. Here we describe the three-dimensional structure of rapidly frozen axonemes from Chlamydomonas and sea urchin sperm, using cryoelectron tomography and image processing to focus on the motor enzyme dynein. Our images suggest a model for the way dynein generates force to slide microtubules. They also reveal two dynein linkers that may provide "hard-wiring" to coordinate motor enzyme action, both circumferentially and along the axoneme. Periodic densities were also observed inside doublet microtubules; these may contribute to doublet stability. |
| doi_str_mv | 10.1126/science.1128618 |
| format | Article |
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Periodic densities were also observed inside doublet microtubules; these may contribute to doublet stability.</description><identifier>ISSN: 0036-8075</identifier><identifier>EISSN: 1095-9203</identifier><identifier>DOI: 10.1126/science.1128618</identifier><identifier>PMID: 16917055</identifier><identifier>CODEN: SCIEAS</identifier><language>eng</language><publisher>Washington, DC: American Association for the Advancement of Science</publisher><subject>Animals ; Atoms ; Biological and medical sciences ; Carrier Proteins - chemistry ; Carrier Proteins - ultrastructure ; Cell motility ; Cell structures and functions ; Chlamydomonas ; Chlamydomonas reinhardtii - ultrastructure ; Cryoelectron Microscopy ; Dyneins - chemistry ; Dyneins - physiology ; Dyneins - ultrastructure ; Echinoidea ; Electrons ; Enzymes ; Eukaryotes ; Flagella ; Flagella - chemistry ; Flagella - physiology ; Flagella - ultrastructure ; Freezing ; Fundamental and applied biological sciences. 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Richard</creatorcontrib><title>Molecular Architecture of Axonemes Revealed by Cryoelectron Tomography</title><title>Science (American Association for the Advancement of Science)</title><addtitle>Science</addtitle><description>Eukaryotic flagella and cilia are built on a 9 + 2 array of microtubules plus >250 accessory proteins, forming a biological machine called the axoneme. Here we describe the three-dimensional structure of rapidly frozen axonemes from Chlamydomonas and sea urchin sperm, using cryoelectron tomography and image processing to focus on the motor enzyme dynein. Our images suggest a model for the way dynein generates force to slide microtubules. They also reveal two dynein linkers that may provide "hard-wiring" to coordinate motor enzyme action, both circumferentially and along the axoneme. Periodic densities were also observed inside doublet microtubules; these may contribute to doublet stability.</description><subject>Animals</subject><subject>Atoms</subject><subject>Biological and medical sciences</subject><subject>Carrier Proteins - chemistry</subject><subject>Carrier Proteins - ultrastructure</subject><subject>Cell motility</subject><subject>Cell structures and functions</subject><subject>Chlamydomonas</subject><subject>Chlamydomonas reinhardtii - ultrastructure</subject><subject>Cryoelectron Microscopy</subject><subject>Dyneins - chemistry</subject><subject>Dyneins - physiology</subject><subject>Dyneins - ultrastructure</subject><subject>Echinoidea</subject><subject>Electrons</subject><subject>Enzymes</subject><subject>Eukaryotes</subject><subject>Flagella</subject><subject>Flagella - chemistry</subject><subject>Flagella - physiology</subject><subject>Flagella - ultrastructure</subject><subject>Freezing</subject><subject>Fundamental and applied biological sciences. 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| fulltext | fulltext |
| identifier | ISSN: 0036-8075 |
| ispartof | Science (American Association for the Advancement of Science), 2006-08, Vol.313 (5789), p.944-948 |
| issn | 0036-8075 1095-9203 |
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| source | MEDLINE; Science Magazine; Jstor Complete Legacy |
| subjects | Animals Atoms Biological and medical sciences Carrier Proteins - chemistry Carrier Proteins - ultrastructure Cell motility Cell structures and functions Chlamydomonas Chlamydomonas reinhardtii - ultrastructure Cryoelectron Microscopy Dyneins - chemistry Dyneins - physiology Dyneins - ultrastructure Echinoidea Electrons Enzymes Eukaryotes Flagella Flagella - chemistry Flagella - physiology Flagella - ultrastructure Freezing Fundamental and applied biological sciences. Psychology Image Processing, Computer-Assisted Imaging, Three-Dimensional Male Materials Microtubule-Associated Proteins Microtubules Microtubules - chemistry Microtubules - physiology Microtubules - ultrastructure Miscellaneous Models, Biological Molecular and cellular biology Molecular Motor Proteins - chemistry Molecular Motor Proteins - ultrastructure Molecular structure Periodicity Protein Structure, Tertiary Proteins Sea Urchins Sperm Tail - chemistry Sperm Tail - physiology Sperm Tail - ultrastructure Spermatozoa Tomography |
| title | Molecular Architecture of Axonemes Revealed by Cryoelectron Tomography |
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