Number and Function of Endothelial Progenitor Cells as a Marker of Severity for Diabetic Vasculopathy
OBJECTIVE—Peripheral arterial disease (PAD) is a threatening complication of diabetes. As endothelial progenitor cells (EPCs) are involved in neovasculogenesis and maintenance of vascular homeostasis, their impairment may have a role in the pathogenesis of diabetic vasculopathy. This study aimed to...
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| Veröffentlicht in: | Arteriosclerosis, thrombosis, and vascular biology thrombosis, and vascular biology, 2006-09, Vol.26 (9), p.2140-2146 |
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| creator | Fadini, Gian Paolo Sartore, Saverio Albiero, Mattia Baesso, Ilenia Murphy, Ellen Menegolo, Mirko Grego, Franco de Kreutzenberg, Saula Vigili Tiengo, Antonio Agostini, Carlo Avogaro, Angelo |
| description | OBJECTIVE—Peripheral arterial disease (PAD) is a threatening complication of diabetes. As endothelial progenitor cells (EPCs) are involved in neovasculogenesis and maintenance of vascular homeostasis, their impairment may have a role in the pathogenesis of diabetic vasculopathy. This study aimed to establish whether number and function of EPCs correlate with PAD severity in type 2 diabetic patients.
METHODS AND RESULTS—EPCs were defined by the expression of CD34, CD133 and KDR, and quantified by flow cytometry in 127 diabetic patients with and without PAD. PAD severity has been assessed as carotid atherosclerosis and clinical stage of leg atherosclerosis obliterans. Diabetic patients with PAD displayed a significant 53% reduction in circulating EPCs versus non-PAD patients, and EPC levels were negatively correlated with the degree of carotid stenosis and the stage of leg claudication. Moreover, the clonogenic and adhesion capacity of cultured EPCs were significantly lower in diabetic patients with PAD versus patients without.
CONCLUSIONS—This study demonstrates that EPC decrease is related to PAD severity and that EPC function is altered in diabetic subjects with PAD, strengthening the pathogenetic role of EPC dysregulation in diabetic vasculopathy. EPC count may be considered a novel biological marker of peripheral atherosclerosis in diabetes. |
| doi_str_mv | 10.1161/01.ATV.0000237750.44469.88 |
| format | Article |
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METHODS AND RESULTS—EPCs were defined by the expression of CD34, CD133 and KDR, and quantified by flow cytometry in 127 diabetic patients with and without PAD. PAD severity has been assessed as carotid atherosclerosis and clinical stage of leg atherosclerosis obliterans. Diabetic patients with PAD displayed a significant 53% reduction in circulating EPCs versus non-PAD patients, and EPC levels were negatively correlated with the degree of carotid stenosis and the stage of leg claudication. Moreover, the clonogenic and adhesion capacity of cultured EPCs were significantly lower in diabetic patients with PAD versus patients without.
CONCLUSIONS—This study demonstrates that EPC decrease is related to PAD severity and that EPC function is altered in diabetic subjects with PAD, strengthening the pathogenetic role of EPC dysregulation in diabetic vasculopathy. EPC count may be considered a novel biological marker of peripheral atherosclerosis in diabetes.</description><identifier>ISSN: 1079-5642</identifier><identifier>EISSN: 1524-4636</identifier><identifier>DOI: 10.1161/01.ATV.0000237750.44469.88</identifier><identifier>PMID: 16857948</identifier><identifier>CODEN: ATVBFA</identifier><language>eng</language><publisher>Philadelphia, PA: American Heart Association, Inc</publisher><subject>Aged ; Arteries ; Associated diseases and complications ; Atherosclerosis (general aspects, experimental research) ; Biological and medical sciences ; Blood and lymphatic vessels ; Blood Cells - pathology ; Blood vessels and receptors ; Cardiology. Vascular system ; Carotid Stenosis - pathology ; Carotid Stenosis - physiopathology ; Case-Control Studies ; Cell Adhesion ; Cell Count ; Cells, Cultured ; Colony-Forming Units Assay ; Diabetes Mellitus, Type 2 ; Diabetes. Impaired glucose tolerance ; Diabetic Angiopathies - pathology ; Diabetic Angiopathies - physiopathology ; Diseases of the peripheral vessels. Diseases of the vena cava. Miscellaneous ; Endocrine pancreas. Apud cells (diseases) ; Endocrinopathies ; Endothelial Cells - pathology ; Female ; Flow Cytometry ; Fundamental and applied biological sciences. Psychology ; Humans ; Intermittent Claudication - pathology ; Intermittent Claudication - physiopathology ; Leg - blood supply ; Male ; Medical sciences ; Peripheral Vascular Diseases - pathology ; Peripheral Vascular Diseases - physiopathology ; Severity of Illness Index ; Stem Cells - pathology ; Vertebrates: cardiovascular system</subject><ispartof>Arteriosclerosis, thrombosis, and vascular biology, 2006-09, Vol.26 (9), p.2140-2146</ispartof><rights>2006 American Heart Association, Inc.</rights><rights>2006 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c6012-3afc7dc7cdd21f5cd95df071bf4c781d64e1e90fdb77b3aefdda17446c09a2793</citedby><cites>FETCH-LOGICAL-c6012-3afc7dc7cdd21f5cd95df071bf4c781d64e1e90fdb77b3aefdda17446c09a2793</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18066789$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16857948$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fadini, Gian Paolo</creatorcontrib><creatorcontrib>Sartore, Saverio</creatorcontrib><creatorcontrib>Albiero, Mattia</creatorcontrib><creatorcontrib>Baesso, Ilenia</creatorcontrib><creatorcontrib>Murphy, Ellen</creatorcontrib><creatorcontrib>Menegolo, Mirko</creatorcontrib><creatorcontrib>Grego, Franco</creatorcontrib><creatorcontrib>de Kreutzenberg, Saula Vigili</creatorcontrib><creatorcontrib>Tiengo, Antonio</creatorcontrib><creatorcontrib>Agostini, Carlo</creatorcontrib><creatorcontrib>Avogaro, Angelo</creatorcontrib><title>Number and Function of Endothelial Progenitor Cells as a Marker of Severity for Diabetic Vasculopathy</title><title>Arteriosclerosis, thrombosis, and vascular biology</title><addtitle>Arterioscler Thromb Vasc Biol</addtitle><description>OBJECTIVE—Peripheral arterial disease (PAD) is a threatening complication of diabetes. As endothelial progenitor cells (EPCs) are involved in neovasculogenesis and maintenance of vascular homeostasis, their impairment may have a role in the pathogenesis of diabetic vasculopathy. This study aimed to establish whether number and function of EPCs correlate with PAD severity in type 2 diabetic patients.
METHODS AND RESULTS—EPCs were defined by the expression of CD34, CD133 and KDR, and quantified by flow cytometry in 127 diabetic patients with and without PAD. PAD severity has been assessed as carotid atherosclerosis and clinical stage of leg atherosclerosis obliterans. Diabetic patients with PAD displayed a significant 53% reduction in circulating EPCs versus non-PAD patients, and EPC levels were negatively correlated with the degree of carotid stenosis and the stage of leg claudication. Moreover, the clonogenic and adhesion capacity of cultured EPCs were significantly lower in diabetic patients with PAD versus patients without.
CONCLUSIONS—This study demonstrates that EPC decrease is related to PAD severity and that EPC function is altered in diabetic subjects with PAD, strengthening the pathogenetic role of EPC dysregulation in diabetic vasculopathy. EPC count may be considered a novel biological marker of peripheral atherosclerosis in diabetes.</description><subject>Aged</subject><subject>Arteries</subject><subject>Associated diseases and complications</subject><subject>Atherosclerosis (general aspects, experimental research)</subject><subject>Biological and medical sciences</subject><subject>Blood and lymphatic vessels</subject><subject>Blood Cells - pathology</subject><subject>Blood vessels and receptors</subject><subject>Cardiology. Vascular system</subject><subject>Carotid Stenosis - pathology</subject><subject>Carotid Stenosis - physiopathology</subject><subject>Case-Control Studies</subject><subject>Cell Adhesion</subject><subject>Cell Count</subject><subject>Cells, Cultured</subject><subject>Colony-Forming Units Assay</subject><subject>Diabetes Mellitus, Type 2</subject><subject>Diabetes. Impaired glucose tolerance</subject><subject>Diabetic Angiopathies - pathology</subject><subject>Diabetic Angiopathies - physiopathology</subject><subject>Diseases of the peripheral vessels. Diseases of the vena cava. Miscellaneous</subject><subject>Endocrine pancreas. Apud cells (diseases)</subject><subject>Endocrinopathies</subject><subject>Endothelial Cells - pathology</subject><subject>Female</subject><subject>Flow Cytometry</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Intermittent Claudication - pathology</subject><subject>Intermittent Claudication - physiopathology</subject><subject>Leg - blood supply</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Peripheral Vascular Diseases - pathology</subject><subject>Peripheral Vascular Diseases - physiopathology</subject><subject>Severity of Illness Index</subject><subject>Stem Cells - pathology</subject><subject>Vertebrates: cardiovascular system</subject><issn>1079-5642</issn><issn>1524-4636</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkF1vFCEUhonR2Fr9C4aY6N2MfA0w3jVrqyb1I7H2ljBwcMeywxZmbPbfy7qbLIEAyfNyDg9CbyhpKZX0PaHt5e1dS-pgXKmOtEII2bdaP0HntGOiEZLLp_VMVN90UrAz9KKUP5UXjJHn6IxK3ale6HME35bNABnbyePrZXLzmCacAr6afJrXEEcb8Y-cfsM0zinjFcRYsK0Tf7X5vgYr-xP-Qh7nHQ6V-DjaAebR4Ttb3BLT1s7r3Uv0LNhY4NVxv0C_rq9uV5-bm--fvqwubxonCWUNt8Ep75TzntHQOd93PhBFhyCc0tRLARR6Evyg1MAtBO8tVfXvjvSWqZ5foHeHd7c5PSxQZrMZi6s92wnSUozUShJNeQU_HECXUykZgtnmcWPzzlBi9pINoaZKNifJ5r9ko3UNvz5WWYYN-FP0aLUCb49AdWBjyHZyYzlxmkip9L5dceAeU5whl_u4PEI2a7BxXu9LCy5J1zBCJOnrtamLM_4PnoaWfQ</recordid><startdate>200609</startdate><enddate>200609</enddate><creator>Fadini, Gian Paolo</creator><creator>Sartore, Saverio</creator><creator>Albiero, Mattia</creator><creator>Baesso, Ilenia</creator><creator>Murphy, Ellen</creator><creator>Menegolo, Mirko</creator><creator>Grego, Franco</creator><creator>de Kreutzenberg, Saula Vigili</creator><creator>Tiengo, Antonio</creator><creator>Agostini, Carlo</creator><creator>Avogaro, Angelo</creator><general>American Heart Association, Inc</general><general>Lippincott</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200609</creationdate><title>Number and Function of Endothelial Progenitor Cells as a Marker of Severity for Diabetic Vasculopathy</title><author>Fadini, Gian Paolo ; Sartore, Saverio ; Albiero, Mattia ; Baesso, Ilenia ; Murphy, Ellen ; Menegolo, Mirko ; Grego, Franco ; de Kreutzenberg, Saula Vigili ; Tiengo, Antonio ; Agostini, Carlo ; Avogaro, Angelo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c6012-3afc7dc7cdd21f5cd95df071bf4c781d64e1e90fdb77b3aefdda17446c09a2793</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Aged</topic><topic>Arteries</topic><topic>Associated diseases and complications</topic><topic>Atherosclerosis (general aspects, experimental research)</topic><topic>Biological and medical sciences</topic><topic>Blood and lymphatic vessels</topic><topic>Blood Cells - pathology</topic><topic>Blood vessels and receptors</topic><topic>Cardiology. Vascular system</topic><topic>Carotid Stenosis - pathology</topic><topic>Carotid Stenosis - physiopathology</topic><topic>Case-Control Studies</topic><topic>Cell Adhesion</topic><topic>Cell Count</topic><topic>Cells, Cultured</topic><topic>Colony-Forming Units Assay</topic><topic>Diabetes Mellitus, Type 2</topic><topic>Diabetes. Impaired glucose tolerance</topic><topic>Diabetic Angiopathies - pathology</topic><topic>Diabetic Angiopathies - physiopathology</topic><topic>Diseases of the peripheral vessels. Diseases of the vena cava. Miscellaneous</topic><topic>Endocrine pancreas. Apud cells (diseases)</topic><topic>Endocrinopathies</topic><topic>Endothelial Cells - pathology</topic><topic>Female</topic><topic>Flow Cytometry</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Intermittent Claudication - pathology</topic><topic>Intermittent Claudication - physiopathology</topic><topic>Leg - blood supply</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Peripheral Vascular Diseases - pathology</topic><topic>Peripheral Vascular Diseases - physiopathology</topic><topic>Severity of Illness Index</topic><topic>Stem Cells - pathology</topic><topic>Vertebrates: cardiovascular system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fadini, Gian Paolo</creatorcontrib><creatorcontrib>Sartore, Saverio</creatorcontrib><creatorcontrib>Albiero, Mattia</creatorcontrib><creatorcontrib>Baesso, Ilenia</creatorcontrib><creatorcontrib>Murphy, Ellen</creatorcontrib><creatorcontrib>Menegolo, Mirko</creatorcontrib><creatorcontrib>Grego, Franco</creatorcontrib><creatorcontrib>de Kreutzenberg, Saula Vigili</creatorcontrib><creatorcontrib>Tiengo, Antonio</creatorcontrib><creatorcontrib>Agostini, Carlo</creatorcontrib><creatorcontrib>Avogaro, Angelo</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Arteriosclerosis, thrombosis, and vascular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fadini, Gian Paolo</au><au>Sartore, Saverio</au><au>Albiero, Mattia</au><au>Baesso, Ilenia</au><au>Murphy, Ellen</au><au>Menegolo, Mirko</au><au>Grego, Franco</au><au>de Kreutzenberg, Saula Vigili</au><au>Tiengo, Antonio</au><au>Agostini, Carlo</au><au>Avogaro, Angelo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Number and Function of Endothelial Progenitor Cells as a Marker of Severity for Diabetic Vasculopathy</atitle><jtitle>Arteriosclerosis, thrombosis, and vascular biology</jtitle><addtitle>Arterioscler Thromb Vasc Biol</addtitle><date>2006-09</date><risdate>2006</risdate><volume>26</volume><issue>9</issue><spage>2140</spage><epage>2146</epage><pages>2140-2146</pages><issn>1079-5642</issn><eissn>1524-4636</eissn><coden>ATVBFA</coden><abstract>OBJECTIVE—Peripheral arterial disease (PAD) is a threatening complication of diabetes. As endothelial progenitor cells (EPCs) are involved in neovasculogenesis and maintenance of vascular homeostasis, their impairment may have a role in the pathogenesis of diabetic vasculopathy. This study aimed to establish whether number and function of EPCs correlate with PAD severity in type 2 diabetic patients.
METHODS AND RESULTS—EPCs were defined by the expression of CD34, CD133 and KDR, and quantified by flow cytometry in 127 diabetic patients with and without PAD. PAD severity has been assessed as carotid atherosclerosis and clinical stage of leg atherosclerosis obliterans. Diabetic patients with PAD displayed a significant 53% reduction in circulating EPCs versus non-PAD patients, and EPC levels were negatively correlated with the degree of carotid stenosis and the stage of leg claudication. Moreover, the clonogenic and adhesion capacity of cultured EPCs were significantly lower in diabetic patients with PAD versus patients without.
CONCLUSIONS—This study demonstrates that EPC decrease is related to PAD severity and that EPC function is altered in diabetic subjects with PAD, strengthening the pathogenetic role of EPC dysregulation in diabetic vasculopathy. EPC count may be considered a novel biological marker of peripheral atherosclerosis in diabetes.</abstract><cop>Philadelphia, PA</cop><cop>Hagerstown, MD</cop><pub>American Heart Association, Inc</pub><pmid>16857948</pmid><doi>10.1161/01.ATV.0000237750.44469.88</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Arteriosclerosis, thrombosis, and vascular biology, 2006-09, Vol.26 (9), p.2140-2146 |
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| subjects | Aged Arteries Associated diseases and complications Atherosclerosis (general aspects, experimental research) Biological and medical sciences Blood and lymphatic vessels Blood Cells - pathology Blood vessels and receptors Cardiology. Vascular system Carotid Stenosis - pathology Carotid Stenosis - physiopathology Case-Control Studies Cell Adhesion Cell Count Cells, Cultured Colony-Forming Units Assay Diabetes Mellitus, Type 2 Diabetes. Impaired glucose tolerance Diabetic Angiopathies - pathology Diabetic Angiopathies - physiopathology Diseases of the peripheral vessels. Diseases of the vena cava. Miscellaneous Endocrine pancreas. Apud cells (diseases) Endocrinopathies Endothelial Cells - pathology Female Flow Cytometry Fundamental and applied biological sciences. Psychology Humans Intermittent Claudication - pathology Intermittent Claudication - physiopathology Leg - blood supply Male Medical sciences Peripheral Vascular Diseases - pathology Peripheral Vascular Diseases - physiopathology Severity of Illness Index Stem Cells - pathology Vertebrates: cardiovascular system |
| title | Number and Function of Endothelial Progenitor Cells as a Marker of Severity for Diabetic Vasculopathy |
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