High-frequency homologous recombination in plants mediated by zinc-finger nucleases

Homologous recombination offers great promise for plant genome engineering. This promise has not been realized, however, because when DNA enters plant cells homologous recombination occurs infrequently and random integration predominates. Using a tobacco test system, we demonstrate that chromosome b...

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Veröffentlicht in:	The Plant journal : for cell and molecular biology 2005-11, Vol.44 (4), p.693-705
Hauptverfasser:	Wright, D.A, Townsend, J.A, Winfrey, R.J. Jr, Irwin, P.A, Rajagopal, J, Lonosky, P.M, Hall, B.D, Jondle, M.D, Voytas, D.F
Format:	Artikel
Sprache:	eng
Schlagworte:	Base Sequence beta-glucuronidase Biological and medical sciences chromosome breakage Deoxyribonucleases, Type II Site-Specific - genetics Deoxyribonucleases, Type II Site-Specific - metabolism DNA, Plant - genetics DNA, Plant - metabolism double‐strand breaks Fundamental and applied biological sciences. Psychology gene targeting Genes, Reporter Genetic Engineering genetic recombination Genic rearrangement. Recombination. Transposable element genome engineering Models, Genetic Molecular and cellular biology Molecular genetics molecular sequence data Nicotiana - enzymology Nicotiana - genetics Nicotiana tabacum non‐homologous end joining nucleases nucleotide sequences plant genetics Plants - enzymology Plants - genetics Plants, Genetically Modified protoplast Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - metabolism Recombination, Genetic reporter genes tobacco transgenic plants zinc finger motif Zinc Fingers - genetics
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